Abstract：
:The genetic linkage relationships of the human glycosphingolipid beta-galactosidases were determined using human--mouse somatic cell hybrids. A new method was devised for the estimation of human galactosylceramide, lactosylceramide, and GMI-ganglioside beta-galactosidase activities in the presence of their mouse counterparts, which takes advantage of the reproducible specific activity of lysosomal hydrolases under a given set of culture conditions and is based on differences in both pH optima and sensitivity to chloride ion. Human and mouse chromosomes were identified by their characteristic banding patterns obtained after quinacrine staining, and the optimum glycolipid beta-galactosidase activity was determined for three different substrates. A ratio was defined for each activity which was the specific activity at the human pH optimum divided by the specific activity at the mouse pH optimum. Linear regression analysis was used to test for concordant segregation between pH ratios for each enzyme and the frequency of occurrence of different human chromosomes in the man--mouse somatic hybrid clones. The results obtained from two independent series of hybrid clones indicated that human beta-galactosidase activities consistently segregated with human chromosome 12 in these somatic cell hybrids.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Rushton AR,Dawson Gdoi
10.1007/BF00484498subject
Has Abstractpub_date
1977-12-01 00:00:00pages
1071-82issue
11-12eissn
0006-2928issn
1573-4927journal_volume
15pub_type
杂志文章abstract：:A mouse mutant with pyruvate kinase (PK) hyperactivity has been found in offspring of 1-ethyl-1-nitrosourea (ENU)-treated male mice. The activity alteration was detected in the blood and could also be found in the liver but not in the muscle, kidney, heart, spleen, lung, or brain. Heterozygous mice have erythrocyte PK...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499635
更新日期：1984-12-01 00:00:00
abstract：:Among the European fauna, the Sardinian hare (Lepus sp.) is peculiar in that it differs from all other hares inhabiting the continent. Here, we report on the variation of a 461 bp sequence of hypervariable domain 1 of the mitochondrial control region, examined in 42 hares collected throughout Sardinia and compared to ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-007-9076-z
更新日期：2007-04-01 00:00:00
abstract：:DNA damage repair elements accumulate at DNA damage sites to form ionizing radiation-induced foci (IRIF) for damage repair. IRIF, which represent direct evidence of DNA damage response activity, which are conveniently to be observed via immunofluorescence staining. Protein ubiquitination plays an important role in ini...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-015-9707-8
更新日期：2016-04-01 00:00:00
abstract：:In flowers of Dianthus caryophyllus (carnation), the gene I is concerned with a discrete step in flavonoid biosynthesis, Genotypes with recessive (ii) alleles produce yellow flowers, which contain the chalcone isosalipurposide (naringenin-chalcone-2'-glucoside) as the major petal pigment, but in genotypes with wild-ty...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484399
更新日期：1980-06-01 00:00:00
abstract：:The murine submandibular gland (SMG) produces a novel class of highly acidic salivary proteins encoded by one or more highly abundant mRNA transcripts. In inbred mice, these transcripts are encoded by members of a multigene family comprising approximately 8-12 homologues. Most, and probably all, of these homologues ar...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02396156
更新日期：1989-10-01 00:00:00
abstract：:The genetic differentiation among the populations of the European abalone Haliotis tuberculata was investigated using different markers to better understand the evolutionary history and exchanges between populations. Three markers were used: mitochondrial cytochrome oxidase I (COI), the sperm lysin nuclear gene, and e...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-016-9778-1
更新日期：2017-04-01 00:00:00
abstract：:In order to clarify the genetic linkage groups of the Japanese brown frog Rana japonica, and compare them with those of other vertebrates, the inheritance of 10 enzyme and blood protein loci was examined in 267 offspring derived from 18 crosses using 10 males heterozygous at these loci. Most of the segregation tests e...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554413
更新日期：1996-10-01 00:00:00
abstract：:Neurospora crassa can utilize numerous compounds including certain amino acids as a sole nitrogen source. Mutants of the nit-2 locus, a regulatory gene which is postulated to mediate nitrogen catabolite repression, are deficient in the ability to utilize several amino acids as well as other nitrogen sources used by wi...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484090
更新日期：1978-04-01 00:00:00
abstract：:Arylsulfatase A (ASA) activity was studied from five blood fractions, leukocytes plus platelets (LKPL), leukocytes (LK), neutrophils (N), lymphocytes (LYM), and platelets (PL). No significant difference was found among the mean ASA specific activity values for the fractions. Electrophoretic examination revealed four d...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484068
更新日期：1982-10-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00483979
更新日期：1977-10-01 00:00:00
abstract：:A new variant of hereditary hemolytic anemia in a family due to high adenosine triphosphate (ATP) is reported. The increase in ATP levels varied from 83 to 105% in the family members. Low 2,3-diphosphoglycerate levels and low 2,3-diphosphoglyceromutase activity were observed in three family members, with normal glucos...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00504264
更新日期：1981-10-01 00:00:00
abstract：:Electrophoretic variation is described for malic enzyme (ME) for the first time in brook trout (Salvelinus fontinalis). Since the quaternary structure of ME was not clear from examination of banding patterns in brook trout alone, ME phenotypes in rainbow trout (Salmo gairdneri)X brook trout hybrids as wel as in esoci...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00502121
更新日期：1979-08-01 00:00:00
abstract：:Maternal embryo leucine zipper kinase (MELK) has a higher expression level in a variety of cancers and involved in progression of colorectal cancer. The MELK expression levels in colorectal cancer tissues and cells were detected by RT-qPCR. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and transwe...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-020-09974-x
更新日期：2020-10-01 00:00:00
abstract：:The single-strand conformation polymorphism (SSCP) procedure has been applied in routine testing for hereditary diseases. Temperature, running buffer, gel composition, and fragment length can influence its sensitivity. Mutation detection in the clinical setting depends on the development of automated technology, espec...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-007-9109-7
更新日期：2007-10-01 00:00:00
abstract：:Alcohol dehydrogenase class IV (ADH4) participates in retinol metabolism and is expressed primarily in ocular, digestive, and reproductive tissues of the mouse. A naturally occurring genetic variant in C57BL/6J mice results in a faster migrating ADH4 enzyme during electrophoresis when compared to other non-C57BJ/6J st...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1010278631535
更新日期：2001-08-01 00:00:00
abstract：:beta-Glucosidase (beta-D-glucoside glucohydrolase, E.C. 3.2.1.21, beta-Glu) isozyme variants were studied in a large number of inbred lines, crosses, and races of maize (Zea mays L.). The pattern of Mendelian inheritance demonstrated for beta-GLU variants indicated that they are under nuclear gene control. Twenty-two ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484468
更新日期：1977-04-01 00:00:00
abstract：:Mitochondrial DNA (mtDNA) hypervariable segment I sequences (HVSI, 471 bp) of the control region and partial cytochrome b sequences (Cytb, 403 bp) were analyzed in three tentative species of the genus Mystacoleucus in China (M. chilopterus, M. marginatus, and M. lepturus). Not more than two mutations were found in bot...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/b:bigi.0000006031.20967.08
更新日期：2003-10-01 00:00:00
abstract：:A bioautographic procedure has been developed for the visualization of the isozymes of dihydrofolate reductase (DHFR, E.C. 1.5.1.3). In addition to detecting electrophoretically separated enzymes, bioautography was utilized to visualize DHFR after isoelectric focusing on polyacrylamide gels. Both zone electrophoresis ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00504369
更新日期：1980-02-01 00:00:00
abstract：:The ratio of cellular RNA and protein content is about 1:1 between phylogenetically diploid and tetraploid species of the teleost family Cyprinidae, but is roughly in proportion to ploidy in species of the teleost order Isospondyli. Enzyme activities do not unequivocally comply with this scheme. These findings are dis...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00485129
更新日期：1976-12-01 00:00:00
abstract：:Oral cavity cancer belongs to head-and-neck squamous cell carcinoma group. The purpose of the study was to assess the levels of certain proteins in a tumour and surgical margin in a group of patients with oral cavity cancer. The levels of DAPK1, MGMT, CDH1, SFRP1, SFRP2, RORA, TIMP3, p16, APC and RASSF1 proteins were ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-017-9799-4
更新日期：2017-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-008-9153-y
更新日期：2008-06-01 00:00:00
abstract：:Starch gel electrophoresis and histochemical staining with L-leucylglycyl-glycine revealed genetic polymorphism in peptidase B in cattle erythrocytes. Genetic analysis of 164 full families indicated that the three phenotypes observed are the product of an autosomal locus with two codominant alleles, PepB1 and PepB2. A...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：:Animal melanin has an important role in the formation of animal fur and skin, which is determined by its quantities, character, and distribution. To identify the effect of melanin on the formation of multi-colored Rex rabbits (Black, Chinchilla, Beaver, Protein cyan, Protein yellow, White), the structure of hair folli...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-019-09929-x
更新日期：2019-10-01 00:00:00
abstract：:Studies have investigated the association between angiotensin-converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism and cardiometabolic risk factors (CMRFs), however with varying results, which could be due to ethnicity differences. Therefore, the present study was conducted among Bhil tribal population (...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-018-9845-x
更新日期：2018-08-01 00:00:00
abstract：:A variant of fetal hemoglobin (Hb F-Malta-I) has been detected and quantitated in adult blood with a sensitive radioimmunoassay employing monospecific antisera. The concentration of Hb-F-Malta-I was 0.002-0.05%, with an average value of 0.011%. The ratio of Hb F-Malta-I/Hb F in adults was about 4.8%, compared to a rat...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00483988
更新日期：1977-10-01 00:00:00
abstract：:Akt isoforms have critical roles in the cause and regulation of cancer cells invasive, migration, and metastatic dissemination. In the present study, the association between Akt1 polymorphisms and endometrial cancer was investigated in patients with endometrial cancer and controls. Thirty premenopaused patients diagno...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-015-9690-0
更新日期：2015-12-01 00:00:00
abstract：:Preeclampsia leads to maternal and perinatal morbidity and mortality. The literature in English was reviewed to summarize recent advances in understanding the global gene expression changes in the pathogenesis of preeclampsia. We identified at least eight consistently enriched categories, relating to pregnancy mainten...
journal_title：Biochemical genetics
pub_type： 杂志文章,评审
doi：10.1007/s10528-015-9684-y
更新日期：2015-10-01 00:00:00
abstract：:Phosphoglucomutase (PGM) of adult stage in Drosophilia melanogaster has been characterized by gel filtration, ion-exchange chromatography, and isoelectric focusing. The two common electrophoretic variants, PGMA and PGMB, differ with respect to their kinetic and stability parameters. PGMA is more thermostable than PGMB...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00504306
更新日期：1979-10-01 00:00:00
abstract：:The rice cultivar Hikarishinseiki, a semidwarf isogenotype of Koshihikari carrying the Green Revolution sd1 gene, is increasingly grown in both Japan and the United States. Here, we report DNA diagnosis for Hikarishinseiki targeting its Jukkoku-type sd1 locus, which codes for a defective gibberellin 20-oxidase, with a...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-013-9584-y
更新日期：2013-08-01 00:00:00
abstract：:Myocardial infarction (MI) is common in India and the disease occurs at a relatively younger age. We wanted to look for association of Angiotensin I-converting enzyme (ACE) gene with MI in North East India. We also wanted to examine possible environmental interaction of ACE gene with established cardiovascular risk fa...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-015-9706-9
更新日期：2016-04-01 00:00:00
