Translation of endogenous message in embryonic chick erythroid cell polysomes in a cell-free protein-synthesizing system.

Abstract:

:Polysomes from 5.5-day and 6.5-day embryonic chick erythroid cells contain messenger RNA (mRNA) which can be translated into products in a cell-free protein-synthesizing system. The products of the cell-free system coelectrophoresed with carrier globin chains from the cells from which the polysomal pellet was isolated. Wheat germ S30 fraction increases by 1.5--2.0 fold [3H] leucine incorporation into trichloroacetic acid-precipitable material directed by the endogenous mRNA on chick erythroid cell polysomes. The wheat germ probably provides a specific factor or factors absent in a shortage in the incubation medium.

journal_name

Biochem Genet

journal_title

Biochemical genetics

authors

Zagris N

doi

10.1007/BF00483979

subject

Has Abstract

pub_date

1977-10-01 00:00:00

pages

825-32

issue

9-10

eissn

0006-2928

issn

1573-4927

journal_volume

15

pub_type

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