Abstract：
:Soluble starch synthase and branching enzyme were purified from 18-day-old cotyledons of the smooth-seeded pea cultivar Alaska (RR) and wrinkled-seeded pea cultivar Progress #9 (rr) by DEAE-cellulose chromatography. Two coeluting peaks of primed and citrate-stimulated starch synthase activity and a major and minor peak of branching enzyme activity were observed in Alaska. However, in Progress #9, only one peak of synthase activity was found. When crude extracts of Progress #9 were centrifuged, over 70% of the starch synthase activity was recovered in the pelleted fraction, and additional washings of the pellet released no further activity. The addition of purified starch granules to Alaska crude extracts also resulted in the recovery of a greater proportion of synthase activity in pelleted fractions. The two peaks of branching enzyme activity in Alaska differed in their stimulation of phosphorylase, amylose branching activity, and activity in various buffers. The DEAE-cellulose profile of Progress #9 showed no distinct peak of branching enzyme and less than 10% of the total activity found in Alaska. The association of one form of soluble starch synthase with the pelleted fraction and the greatly reduced levels of branching enzyme provide a partial explanation for the appearance of high-amylose starch in Progress #9 cotyledons.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Matters GL,Boyer CDdoi
10.1007/BF00484061subject
Has Abstractpub_date
1982-10-01 00:00:00pages
833-48issue
9-10eissn
0006-2928issn
1573-4927journal_volume
20pub_type
杂志文章abstract：:A survey of laboratory strains of Anopheles stephensi for nonspecific esterases by polyacrylamide gel electrophoresis revealed 10 zones of esterase activity. In 3 of the 10 zones, three electromorphs were observed. Genetic analysis revealed that these three zones are controlled by three loci, viz., Est-3, Est-4, and E...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484518
更新日期：1984-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00486123
更新日期：1976-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02395516
更新日期：1988-12-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499290
更新日期：1984-02-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499081
更新日期：1985-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-009-9274-y
更新日期：2009-12-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1018781026170
更新日期：1998-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s10528-007-9109-7
更新日期：2007-10-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1020918411532
更新日期：2003-02-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484877
更新日期：1976-02-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484568
更新日期：1981-12-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-009-9316-5
更新日期：2010-04-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499114
更新日期：1985-02-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00485850
更新日期：1984-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00570122
更新日期：1996-12-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484929
更新日期：1975-10-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/b:bigi.0000007777.87407.1b
更新日期：2003-12-01 00:00:00
abstract：:The East Himalaya-Hengduan Mountains region is the center of diversity of the genus Primula, and P. sikkimensis is one of the most common members of the genus in the region. In this study, the genetic diversity and structure of P. sikkimensis populations in China were assessed using inter-simple sequence repeat (ISSR)...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-007-9131-9
更新日期：2008-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s10528-016-9778-1
更新日期：2017-04-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484525
更新日期：1978-12-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00556214
更新日期：1987-10-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554598
更新日期：1995-12-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484206
更新日期：1978-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499943
更新日期：1985-12-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1015841424598
更新日期：2002-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-020-09968-9
更新日期：2020-10-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1002797219182
更新日期：2001-02-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-010-9408-2
更新日期：2011-06-01 00:00:00
abstract：:Gli-D1-encoded omega gliadins of bread wheats show little variation; their electrophoretic patterns can be classified into two main groups which broadly resemble the patterns found in the cultivars Chinese Spring and in Cheyenne. B and D subunits of low molecular weight glutenin encoded by the chromosome 1D loci Glu-D...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554146
更新日期：1991-08-01 00:00:00
