Abstract：
:We describe a simple method for locating tryptophanyl-tRNA synthetase (E.C. 6.1.1.2) on cellulose acetate gels (Cellogel) following electrophoresis. Employing electrophoretic conditions which result in the separation of mouse and human tryptophanyl-tRNA synthetase, we have analyzed extracts of a number of independently derived mouse-human somatic cell hybrids and subclones derived from these hybrids for the presence of human tryptophanyl-tRNA synthetase. Electrophoretic patterns of hybrid extracts which contain human tryptophanyl-tRNA synthetase exhibit three bands. This is consistent with published evidence that the enzyme for mammalian cells is a homologous dimer. The electrophoretic patterns derived from some hybrids are unusual in that the human and hybrid bands of activity are more intense than the mouse band from the same hybrid. An analysis of hybrid cells and extracts indicates that human tryptophanyl-tRNA synthetase segregates with human chromosome 14 and with the only enzyme marker which has previously been assigned to this chromosome, nucleoside phosphorylase.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Denney RM,Craig IWdoi
10.1007/BF00484877subject
Has Abstractpub_date
1976-02-01 00:00:00pages
99-117issue
1-2eissn
0006-2928issn
1573-4927journal_volume
14pub_type
杂志文章abstract：:Imprinted genes are characterized by monoallelic expression that is dependent on parental origin. Comparative analysis of imprinted genes between species is a powerful tool for understanding the biological significance of genomic imprinting. The slc38a4 gene encodes a neutral amino acid transporter and is identified a...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-018-9866-5
更新日期：2018-12-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-020-09968-9
更新日期：2020-10-01 00:00:00
abstract：:When the 4-bp Dam recognition sequence was placed between two d(GA)7 tracts, it became severely undermethylated in JM101 Escherichia coli cells compared to other Dam sequences in the same plasmid DNA. This site specific undermethylation was also detected on supercoiled molecules in vitro. Mutational analysis indicated...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02407017
更新日期：1996-06-01 00:00:00
abstract：:Mitochondrial DNA (mtDNA) hypervariable segment I sequences (HVSI, 471 bp) of the control region and partial cytochrome b sequences (Cytb, 403 bp) were analyzed in three tentative species of the genus Mystacoleucus in China (M. chilopterus, M. marginatus, and M. lepturus). Not more than two mutations were found in bot...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/b:bigi.0000006031.20967.08
更新日期：2003-10-01 00:00:00
abstract：:The retinal pigment epithelium (RPE) plays a key role in the maintenance of the normal functions of the retina. Tissue engineering using amniotic membrane as a substrate to culture RPE cells may provide a promising new strategy to replace damaged RPE. We established a method of culturing RPE cells over the amniotic me...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-010-9409-1
更新日期：2011-06-01 00:00:00
abstract：:The rice cultivar Hikarishinseiki, a semidwarf isogenotype of Koshihikari carrying the Green Revolution sd1 gene, is increasingly grown in both Japan and the United States. Here, we report DNA diagnosis for Hikarishinseiki targeting its Jukkoku-type sd1 locus, which codes for a defective gibberellin 20-oxidase, with a...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-013-9584-y
更新日期：2013-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00486123
更新日期：1976-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-008-9145-y
更新日期：2008-04-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554162
更新日期：1989-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499101
更新日期：1986-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484591
更新日期：1980-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-012-9546-9
更新日期：2013-02-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499476
更新日期：1984-10-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484206
更新日期：1978-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-009-9239-1
更新日期：2009-06-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554349
更新日期：1987-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02426878
更新日期：1993-12-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-011-9426-8
更新日期：2011-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499111
更新日期：1985-02-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-005-8170-3
更新日期：2005-10-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00485135
更新日期：1976-12-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02395516
更新日期：1988-12-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-013-9607-8
更新日期：2013-10-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554142
更新日期：1991-08-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-009-9229-3
更新日期：2009-04-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02401798
更新日期：1989-04-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00557950
更新日期：1995-04-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02399686
更新日期：1991-10-01 00:00:00
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journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484724
更新日期：1978-08-01 00:00:00
abstract：:Polymorphic esterase and acetylcholinesterase alleles in the whitefly Bemisia tabaci were studied using electrophoretic and colorimetric assays. The segregation of these alleles between parental and F1 generations provided unequivocal evidence of haplodiploidy in this pest species. Unmated females, heterozygous at a p...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
