Molecular characterization of the pina gene in einkorn wheat.

Abstract:

:Fifty-six sequences encoding the pina protein were characterized from three species or subspecies of einkorn wheat. These sequences contained 1,595 nucleotides, including 1,270 conserved sites, 21 single nucleotide polymorphisms (SNPs), and 16 indels. The average frequency of SNPs and indels was one out of 76.1 and 99.9 bases, respectively. Five SNPs and no indels were found in the translated sequences. Fourteen haplotypes were defined, and the accessions in each haplotype ranged from 1 to 18. There were nine haplotypes in Triticum monococcum ssp. aegilopoides, eight in T. monococcum ssp. monococcum, and two in T. urartu. Phylogenetic analysis showed that pina genes from different species or subspecies could be clearly differentiated based on the open reading frame. Genes from T. urartu grouped together, whereas genes from T. monococcum ssp. aegilopoides and T. monococcum ssp. monococcum were shared by three and two clusters, respectively. Both the haplotype and phylogenetic analyses indicated that T. monococcum ssp. aegilopoides was more diverse. These results would contribute to the understanding of functional aspects and efficient utilization of pina genes.

journal_name

Biochem Genet

journal_title

Biochemical genetics

authors

Chen Q,Qi PF,Wei YM,Wang JR,Zheng YL

doi

10.1007/s10528-009-9239-1

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

384-96

issue

5-6

eissn

0006-2928

issn

1573-4927

journal_volume

47

pub_type

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