Abstract：
:High levels of nuclease activities were identified in filtrates of Aspergillus cultures after growth in low-but not in high-phosphate media. Deoxyribonuclease activities, characterized extensively by column chromatography, showed a coincident single peak for ss- and ds-DNase which was distinct from the peak for RNase. Both ss-DNase and ds-DNase are endonucleolytic and showed the highest activity in the presence of Ca2+ and Mn2+ (at pH 8.0). They also showed identical heat sensitivities suggesting that a single, phosphate-repressible DNase was secreted. This enzyme, therefore, corresponds to the well-characterized extracellular DNase A of Neurospora. However, the Aspergillus DNase A did not cross-react with antisera to secreted Neurospora nucleases and showed different chromatographic properties, and active peptides of different sizes were visualized on DNA activity gels. The increasing derepression of Aspergillus DNase A by decreasing phosphate levels was similar to that of secreted alkaline phosphatase and these increases were both abolished by the regulatory mutant palcA.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Käfer E,Tittler A,Fraser MJdoi
10.1007/BF02401798subject
Has Abstractpub_date
1989-04-01 00:00:00pages
153-66issue
3-4eissn
0006-2928issn
1573-4927journal_volume
27pub_type
杂志文章abstract：:A major category of mutant hematopoietic phenotypes in Drosophila is melanotic tumors or nodules, which consist of abnormal and overproliferated blood cells, similar to granulomas. Our analyses of the melanotic mutant dappled have revealed a novel type of gene involved in blood cell regulation. The dappled gene is an ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-010-9411-7
更新日期：2011-06-01 00:00:00
abstract：:The murine submandibular gland (SMG) produces a novel class of highly acidic salivary proteins encoded by one or more highly abundant mRNA transcripts. In inbred mice, these transcripts are encoded by members of a multigene family comprising approximately 8-12 homologues. Most, and probably all, of these homologues ar...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02396156
更新日期：1989-10-01 00:00:00
abstract：:Phenol sulfotransferase (PST) catalyzes the sulfate conjugation of catechol and phenolic drugs and xenobiotic compounds. Platelets and other tissues contain at least two forms of PST, forms that have been designated the "TL" and the "TS" forms. We measured the thermal stability of platelet TS PST in blood samples from...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499627
更新日期：1984-12-01 00:00:00
abstract：:Two types of alleles exist in the human alcohol dehydrogenase-2 (ADH2) locus. The usual ADH12 allele is common in Caucasians, while the atypical ADH22 allele is predominant in Orientals. The ADH22 produces the beta 2 subunit, which is catalytically far more active than the beta 1 subunit produced by the ADH12 gene. Th...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02399419
更新日期：1988-08-01 00:00:00
abstract：:Stable reciprocal hybrids between Flaveria pringlei (C3) and F. brownii (C4-like) have been produced by standard breeding techniques. There are no differences in the isoelectric focusing patterns of the catalytic subunits of the ribulose-1,5-bisphosphate carboxylase/oxygenase from F. pringlei, F. brownii, or the recip...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02396147
更新日期：1989-10-01 00:00:00
abstract：:Non-alcoholic fatty liver disease is one of the main causes of chronic liver disease and therefore is currently considered a major public health problem. Sirtuin 1 (SIRT1) is an NAD-dependent deacetylase enzyme that contributes in the regulation of metabolic processes and protects against lipid accumulation in hepatoc...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-019-09905-5
更新日期：2019-08-01 00:00:00
abstract：:Patterns of lactate dehydrogenase (LDH) isozymes of tissues from various mouse strains were examined. An interstrain polymorphism for LDH isozymes of liver was established. One phenotype (CBA/Lac and AKR/J mice) yielded a five-banded LDH pattern, another phenotype (DBA/1J, DBA/2J, C57BL/6J and C3H/He) showed a three-b...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00500133
更新日期：1980-10-01 00:00:00
abstract：:Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes in long-term callus tissue culture of Cereus peruvianus were studied in starch gel electrophoresis to investigate the control of differential Mdh gene expression under sugar and temperature stress. While two cytosol MDH isozymes showed an unchanged phenotype when the ca...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1021950008544
更新日期：1997-06-01 00:00:00
abstract：:Cleavage patterns of mitochondrial DNAs (mtDNAs) by 15 restriction endonucleases were analyzed for 10 swamp and 13 river types of domestic water buffaloes. Digestions with nine enzymes exhibited polymorphisms giving two or three kinds of cleavage patterns. Five mtDNA types were identified, three types in the swamp buf...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554726
更新日期：1995-06-01 00:00:00
abstract：:An electrophoretical analysis of blood plasma proteins of eight Anser and two Branta species was performed. Ten polymorphic proteins in blood plasma pattern were distinguished and described: four prealbumin proteins, albumin, three postalbumin proteins, transferrin, and a single posttransferrin protein. Genus-specific...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00557950
更新日期：1995-04-01 00:00:00
abstract：:An enzyme catalyzing the transfer of the glucosyl moiety of UDP-glucose to the 3-hydroxyl group of cyanidin has been demonstrated in petal extracts of Silene dioica mutants with cyanidin-3-O-glucoside in the petals. This transferase activity was also present in young rosette leaves and calyces of these plants. The hig...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484525
更新日期：1978-12-01 00:00:00
abstract：:Variations at three flight-related enzyme loci, delta-glycerophosphate dehydrogenase (delta-Gpdh), glucose-6-phosphate dehydrogenase (G6pd), and phosphoglucomutase (Pgm), of Epiphyas postvittana (Walker) moths were investigated using starch gel electrophoresis. Among the three enzyme loci, delta-Gpdh and G6pd were fou...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554142
更新日期：1991-08-01 00:00:00
abstract：:Sequence divergence of mitochondrial COII was analyzed in 50 specimens belonging to five subspecies of Polyura eudamippus (Lepidoptera: Nymphalidae) collected from southern China. There were nine haplotypes in the 405 bp of partial COII sequence. Distribution of the five subspecies was primarily consistent with the sp...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-006-9035-0
更新日期：2006-10-01 00:00:00
abstract：:Isoenzyme electrophoresis was used as a method to determine genetic diversity in various M. esculenta cultivars collected in the Southwestern (SW) and Northwestern (NW) regions of the State of Parana, in the South region of Brazil, and in cultivars produced at the Agronomic Institute of Campinas (IAC), São Paulo State...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1001963319965
更新日期：2000-08-01 00:00:00
abstract：:The molecular nature of chicken fetal antigen (CFA) and quail fetal antigen (QFA) was studied on embryonic red blood cells (RBCs) of the chicken, the Japanese quail, and the quail-chicken hybrid. Specific immunoprecipitation of radiolabeled membrane proteins followed by electrophoretic separation and autoradiography w...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499476
更新日期：1984-10-01 00:00:00
abstract：:Arylsulfatase A (ASA) activity was studied from five blood fractions, leukocytes plus platelets (LKPL), leukocytes (LK), neutrophils (N), lymphocytes (LYM), and platelets (PL). No significant difference was found among the mean ASA specific activity values for the fractions. Electrophoretic examination revealed four d...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484068
更新日期：1982-10-01 00:00:00
abstract：:The genetic linkage relationships of the human glycosphingolipid beta-galactosidases were determined using human--mouse somatic cell hybrids. A new method was devised for the estimation of human galactosylceramide, lactosylceramide, and GMI-ganglioside beta-galactosidase activities in the presence of their mouse count...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484498
更新日期：1977-12-01 00:00:00
abstract：:This study deals with the characterization of 10 populations of M. musculus from different geographical locations in India. The genetics of Indian wild mice has been completely obscure and this is the first report on allozyme variations in the natural population. We have used a set of 24 biochemical genetic markers to...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1018781026170
更新日期：1998-08-01 00:00:00
abstract：:Examination of eight Atlantic species of the genus Littorina by starch gel electrophoresis of purine nucleoside phosphorylase revealed extensive polymorphism within the L. saxatilis complex. In this group, four alleles have been identified. Heterozygotes are four banded, and thus, as in vertebrates, the enzyme is like...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00499096
更新日期：1986-06-01 00:00:00
abstract：:Genetic analysis of a polymorphic tissue esterase revealed a new locus (Est-6) with two alleles (Est-6a and Est-6b) on linkage group VI of the rabbit. Est-6 is closely linked to the Est-1,2,4 cluster. Esterase of Est-6 is found in many organs, particularly in liver and small intestine, but not in erythrocytes and seru...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00554543
更新日期：1987-06-01 00:00:00
abstract：:Random amplified polymorphic DNA was used to assess the level of genetic diversity and genetic structure of Stipa krylovii (Gramineae), an important dominant species in the northern grasslands of China. Genetic diversity was low within S. krylovii populations, and diversity at the population level was associated with ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-006-9054-x
更新日期：2006-12-01 00:00:00
abstract：:The heat stability at 56 C of xanthine oxidase (Xox) from mouse intestine was found to be affected by two factors: (1) Xox which had been partially digested by trypsin was less heat stable than Xox which was protected from digestion by the presence of soybean trypsin inhibitor and phenylmethylsulfonyl fluoride; (2) Xo...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484927
更新日期：1975-10-01 00:00:00
abstract：:CCR5 is a seven-transmembrane G-protein-coupled receptor that binds the CC-chemokines including RANTES, eotaxin, MIP-1alpha and beta. CCR5 serves as an essential coreceptor for cell entry of R5 (macrophage-tropic, nonsyncytium-inducing) strains of HIV-1. To date, four deletions have been found in human and primate ccr...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：
更新日期：2005-06-01 00:00:00
abstract：:To investigate the sex differences of crossing-over in Rana japonica, the recombination frequencies between two linked loci, LDH-B and MPI, were examined in offspring obtained from 29 matings involving either females or males heterozygous at both loci. No recombinants were found among 303 offspring bred from 6 heteroz...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF02426898
更新日期：1994-10-01 00:00:00
abstract：:Segregation and linkage relationship of nine allozyme loci, which are fixed for alternative alleles in the European fire-bellied toads, Bombinal bombina and B. variegata, were studied using artificial F1 hybrids to obtain backcross and F2 progeny. Alleles coding for electromorphs at nine loci (Ldh-1, Mdh-1, Idh-1, Ck,...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1023/a:1002032005853
更新日期：2000-08-01 00:00:00
abstract：:Prolamin mixtures were isolated from oats, rice, normal and high-lysine sorghum, two varieties of pearl millet, two strains of teosinte, and gamma grass and subjected to NH2-terminal amino acid sequence determinations. In each case (except for rice, whose prolamins apparently have blocked or unavailable NH2-terminal r...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00498931
更新日期：1982-12-01 00:00:00
abstract：:The molecular weight forms of kynurenine formamidase were studied both gentically and biochemically. Formamidase I (native molecular weight 60,000) was purified using (NH4)2SO4 and pH fractionation, DEAE-cellulose chromatography at two different pH's, hydroxylapatite chromatography, and Sephadex G-100 gel filtration. ...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484718
更新日期：1978-08-01 00:00:00
abstract：:A survey of laboratory strains of Anopheles stephensi for nonspecific esterases by polyacrylamide gel electrophoresis revealed 10 zones of esterase activity. In 3 of the 10 zones, three electromorphs were observed. Genetic analysis revealed that these three zones are controlled by three loci, viz., Est-3, Est-4, and E...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/BF00484518
更新日期：1984-06-01 00:00:00
abstract：:The nuclear factor of activated T lymphocytes (NFATc1) plays a critical role during valvular and septal development. Genetic variants may influence the biological function of the protein and thus play a role in susceptibility to valvuloseptal defects. Tandem repeat polymorphisms and a common nonsynonymous polymorphism...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-011-9434-8
更新日期：2011-10-01 00:00:00
abstract：:In this study, we identified porcine single nucleotide polymorphisms (SNPs) by aligning eight sequences generated with two approaches: amplification of 665 intronic regions using one sample from each of eight breeds, including three East Asian pigs, and amplification of 289 3'-UTR regions using two samples from each o...
journal_title：Biochemical genetics
pub_type： 杂志文章
doi：10.1007/s10528-011-9486-9
更新日期：2012-06-01 00:00:00
