Abstract:
:Nicotiana rustica L. HZNH, a native Chinese tobacco germplasm, displays a hypersensitive response (HR) and systemic acquired resistance following infection with tobacco mosaic virus (TMV). A resistance gene, CN, cloned from HZNH plants, was homologous to the N and NH genes identified in other Nicotiana species. The CN coding region (3423 bp) shares 93.63% and 86.50% nucleotide identity with N and NH, respectively. Whereas the five CN exon sequences are highly homologous with those of N and NH, the four introns differ significantly in length and sequence. Sequence analysis revealed that CN belongs to the TIR/NBS/LRR gene class. Expression of CN was up-regulated after TMV infection and was temperature sensitive. Organ-specific expression analysis suggested that CN transcripts accumulated at high levels in leaves, low levels in stems, and minimal levels in roots. When CN was inserted into TMV-susceptible N. tabacum cv. K326 plants by Agrobacterium-mediated transformation, the transgenic plants displayed HR and systemic HR due to uninhibited movement of the virus.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Zhang GY,Chen M,Guo JM,Xu TW,Li LC,Xu ZS,Ma YZ,Chen XPdoi
10.1007/s10528-009-9229-3subject
Has Abstractpub_date
2009-04-01 00:00:00pages
301-14issue
3-4eissn
0006-2928issn
1573-4927journal_volume
47pub_type
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