Abstract:
:Polymorphic esterase and acetylcholinesterase alleles in the whitefly Bemisia tabaci were studied using electrophoretic and colorimetric assays. The segregation of these alleles between parental and F1 generations provided unequivocal evidence of haplodiploidy in this pest species. Unmated females, heterozygous at a polymorphic locus, produced a 1:1 ratio of haploid males expressing either of the maternal alleles. Although male offspring were produced by both virgin and mated females, the segregation of alleles showed they were always haploid (hemizygous) for the marker enzymes. Females only arose from fertilized eggs and invariably expressed paternal and maternal alleles.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Byrne FJ,Devonshire ALsubject
Has Abstractpub_date
1996-04-01 00:00:00pages
93-107issue
3-4eissn
0006-2928issn
1573-4927journal_volume
34pub_type
杂志文章abstract::Ade-H and ade-I are two auxotrophic mutants of Chinese hamster ovary (CHO-K1) cells which specifically require adenine as the purine source to grow. The enzymatic defects of these mutants were examined in cell-free extracts. It was found that ade-H did not have any detectable adenylosuccinate synthetase activity and a...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484561
更新日期:1977-02-01 00:00:00
abstract::A survey of laboratory strains of Anopheles stephensi for nonspecific esterases by polyacrylamide gel electrophoresis revealed 10 zones of esterase activity. In 3 of the 10 zones, three electromorphs were observed. Genetic analysis revealed that these three zones are controlled by three loci, viz., Est-3, Est-4, and E...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484518
更新日期:1984-06-01 00:00:00
abstract::Three serum transferrin phenotypes of Praomys (Mastomys) natalensis, and African rodent having a high incidence of many varieties of spontaneous neoplasms, were detected by electrophoresis and autoradiography. On electrophoresis, transferrin of Trf-K was found to be a protein that migrates slowly toward the cathode, w...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00485334
更新日期:1976-10-01 00:00:00
abstract::Animal melanin has an important role in the formation of animal fur and skin, which is determined by its quantities, character, and distribution. To identify the effect of melanin on the formation of multi-colored Rex rabbits (Black, Chinchilla, Beaver, Protein cyan, Protein yellow, White), the structure of hair folli...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-019-09929-x
更新日期:2019-10-01 00:00:00
abstract::The genetic divergence, population genetic structure, and possible speciation of the Korean firefly, Pyrocoelia rufa, were investigated on the midsouthern Korean mainland, coastal islets, a remote offshore island, Jedu-do, and Tsushima Island in Japan. Analysis of DNA sequences from the mitochondrial COI protein-codin...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/b:bigi.0000007777.87407.1b
更新日期:2003-12-01 00:00:00
abstract::The variable numbers of tandem repeats (VNTR) locus D1S80, located on chromosome 1 (1p35-36), has a repeat unit 16 bp in length, and different numbers of these repeat units have been observed for populations of different origins and ethnicity. We used a molecular identification method based on capillary electrophoresi...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-009-9250-6
更新日期:2009-08-01 00:00:00
abstract::Three dipeptidases in Drosophila melanogaster are under independent genetic control and their structural genes have been localized, Dip-A to 2R and Dip-B and Dip-C to 3R (Voelker and Langley, 1978; Ohnishi and Voelker, 1981). These enzymes were characterized with respect to their substrate specificities, genetic varia...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484692
更新日期:1982-06-01 00:00:00
abstract::Salvia miltiorrhiza is a well-known medicinal plant and functional food in Asia. The range of quality among its germplasms is irregular, which inevitably affects its health and economic benefits. We established a system comprising phenotypic characteristics, ISSR markers, and active constituents to facilitate the comp...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-013-9600-2
更新日期:2013-10-01 00:00:00
abstract::Eukaryotic translation initiation factors (eIFs) have been shown to be critical in the initiation of protein synthesis. Here, we report the cloning and characterization of a novel gene, LceIF1, from a potentially interesting forage grass, Leymus chinensis (Trin.). The expression results show that LceIF1 is expressed i...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-012-9546-9
更新日期:2013-02-01 00:00:00
abstract::Starch gel electrophoresis and histochemical staining with L-leucylglycyl-glycine revealed genetic polymorphism in peptidase B in cattle erythrocytes. Genetic analysis of 164 full families indicated that the three phenotypes observed are the product of an autosomal locus with two codominant alleles, PepB1 and PepB2. A...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
abstract::The concentration of mouse kidney histidine decarboxylase (HDC) is modulated by estrogen, testosterone, and thyroxine in a tissue-specific manner. Variation in HDC levels between strains of mice can be used to investigate the genetic regulation of enzyme structure, tissue specific expression, and induction and repress...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00485850
更新日期:1984-08-01 00:00:00
abstract::Stable reciprocal hybrids between Flaveria pringlei (C3) and F. brownii (C4-like) have been produced by standard breeding techniques. There are no differences in the isoelectric focusing patterns of the catalytic subunits of the ribulose-1,5-bisphosphate carboxylase/oxygenase from F. pringlei, F. brownii, or the recip...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF02396147
更新日期:1989-10-01 00:00:00
abstract::We have isolated the Drosophila melanogaster locus coding for L-glutamate dehydrogenase (EC 1.4.1.3) by virtue of its similarity to the corresponding human gene. There is only one copy of this gene in the fruit fly genome, located on the right arm of chromosome 3 (95D1-4). The transcript includes at least one large in...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::Nineteen inbred strains of the laboratory rat (Rattus norvegicus) were investigated for genetic differences by starch and agarose gel electrophoretic techniques. For six out of 21 loci, interstrain differences were observed: Tf, 6PGD, Es-1, Es-4, Es-5, and Svp. The variants are briefly described and the allelic distri...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484206
更新日期:1978-06-01 00:00:00
abstract::Type 2 diabetes mellitus (T2DM) is characterized by chronic hyperglycemia associated with insulin resistance and relative insulin deficiency. T2DM is believed to be attributable to the combined effect of genetic and environmental factors. Peroxisome proliferator-activated receptor gamma 2 (PPARγ2) is one of the main c...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-016-9765-6
更新日期:2017-02-01 00:00:00
abstract::DNA polymorphisms in the psi beta 1-beta-globin gene region in nine Asian macaques (Macaca fuscata, M. mulatta, M. nemestrina, M. cyclopis, M. fascicularis, M. arctoides, M. radiata, M. maura, and M. assamensis) were examined using several restriction endonucleasese and the human psi beta 1, delta IVS2, and beta IVS2 ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF02401811
更新日期:1991-04-01 00:00:00
abstract::The retinal pigment epithelium (RPE) plays a key role in the maintenance of the normal functions of the retina. Tissue engineering using amniotic membrane as a substrate to culture RPE cells may provide a promising new strategy to replace damaged RPE. We established a method of culturing RPE cells over the amniotic me...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-010-9409-1
更新日期:2011-06-01 00:00:00
abstract::Alcohol dehydrogenase from Drosophila funebris and D. immigrans is evident at all developmental stages. The highest activity level appears in third-instar larvae and declines to a lower level at all later stages of development. Both species are monomorphic. The enzyme is a dimer consisting of two identical subunits wi...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00499474
更新日期:1984-10-01 00:00:00
abstract::The genetic diversity of the Turkish native chicken breeds Denizli and Gerze was evaluated with 10 microsatellite markers. We genotyped a total of 125 individuals from five subpopulations. Among loci, the mean number of alleles was 7.5, expected heterozygosity (H (e)) was 0.665, PIC value was 0.610, and Wright's fixat...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-008-9164-8
更新日期:2008-08-01 00:00:00
abstract::Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes in long-term callus tissue culture of Cereus peruvianus were studied in starch gel electrophoresis to investigate the control of differential Mdh gene expression under sugar and temperature stress. While two cytosol MDH isozymes showed an unchanged phenotype when the ca...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/a:1021950008544
更新日期:1997-06-01 00:00:00
abstract::A bioautographic procedure has been developed for the visualization of the isozymes of dihydrofolate reductase (DHFR, E.C. 1.5.1.3). In addition to detecting electrophoretically separated enzymes, bioautography was utilized to visualize DHFR after isoelectric focusing on polyacrylamide gels. Both zone electrophoresis ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00504369
更新日期:1980-02-01 00:00:00
abstract::Following starch gel electrophoresis and a modified staining method, three distinct groups of acid phosphatase isozymes (AP-1, AP-2, and AP-3) have been identified in scutellum extracts of different inbred maize lines. The AP-2 group includes three electrophoretic variants: a fast (F), an intermediate (I), and a slow ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00483996
更新日期:1981-08-01 00:00:00
abstract::An electrophoretical analysis of blood plasma proteins of eight Anser and two Branta species was performed. Ten polymorphic proteins in blood plasma pattern were distinguished and described: four prealbumin proteins, albumin, three postalbumin proteins, transferrin, and a single posttransferrin protein. Genus-specific...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00557950
更新日期:1995-04-01 00:00:00
abstract::High levels of nuclease activities were identified in filtrates of Aspergillus cultures after growth in low-but not in high-phosphate media. Deoxyribonuclease activities, characterized extensively by column chromatography, showed a coincident single peak for ss- and ds-DNase which was distinct from the peak for RNase....
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF02401798
更新日期:1989-04-01 00:00:00
abstract::Maternal embryo leucine zipper kinase (MELK) has a higher expression level in a variety of cancers and involved in progression of colorectal cancer. The MELK expression levels in colorectal cancer tissues and cells were detected by RT-qPCR. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and transwe...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-020-09974-x
更新日期:2020-10-01 00:00:00
abstract::This haplotype-based case-control study investigated whether the aldosterone synthase gene (CYP11B2) might be implicated in the pathogenesis of essential hypertension in Yi (226 individuals) and Hani (296 individuals) minorities of China. Four tag SNPs (rs4536, rs4545, rs3097, and rs3802230) and the K173R polymorphism...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-010-9393-5
更新日期:2011-02-01 00:00:00
abstract::Single-chain variable fragment (scFv) is one of the most common antibody forms. This report describes the expression of the scFv gene as a soluble protein in Origami DE3 cytoplasm. The purified scFv recognized the epidermal growth factor receptor (EGFRvIII) on the surface of MCF-7 cells. The scFv protein was purified ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-012-9506-4
更新日期:2012-08-01 00:00:00
abstract::Studies on hybridization, inheritance, and population genetics of brown planthoppers that infest rice and weeds were undertaken using starch gel electrophoresis to determine whether the weed-infesting population represents a biological race or a species. F(1) and F(2) generations were produced by crosses between paren...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-009-9316-5
更新日期:2010-04-01 00:00:00
abstract::Fourteen myoglobins of known sequence were examined by isoelectric focusing with and without urea. The 14 sequences formed six distinct mobility classes on gels without urea and three classes on those with urea. For these proteins, isoelectric focusing provides no advantage over single, nonequilibrium, nondenaturing g...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00504335
更新日期:1986-08-01 00:00:00
abstract::The quantitative and qualitative distributions of gangliosides were determined in the cerebrum, cerebellum, and brain stem of five inbred strains (C57BL/6J, DBA/2J, LG/J, C3H/HeJ, BALB/cJ) of mice at 21 days of age. Genetic differences were found among the strains for wet weight, absolute amount of gangliosides per re...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484473
更新日期:1979-02-01 00:00:00