Abstract:
:Investigations of an euploid series of castor bean, Ricinus communis L., Consisting of haploid, diploid, and tetraploid individuals, was performed to determine the value of such a series in studying the biochemical consequences of genome multiplication. The effects of euploidization of the nuclear genome on the biosynthesis of cellular proteins were examined. Extracts of total soluble proteins from 10-day-old leaves of all three ploidy levels examined by electrophoresis on polyacrylamide gels revealed no difference in the complement of proteins present; however, differences in intensity of several protein bands were detected. Analysis of esterases isozyme activity by isoelectric focusing revealed both increases and decreases in the activity levels of individual isozyme variants in responses to changes in ploidy levels. Results from this analysis are discussed in terms of possible regulatory mechanisms active in the regulation of duplicated genes.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Timko MP,Vasconcelos AC,Fairbrothers DEdoi
10.1007/BF00504367subject
Has Abstractpub_date
1980-02-01 00:00:00pages
171-83issue
1-2eissn
0006-2928issn
1573-4927journal_volume
18pub_type
杂志文章abstract::The molecular nature of chicken fetal antigen (CFA) and quail fetal antigen (QFA) was studied on embryonic red blood cells (RBCs) of the chicken, the Japanese quail, and the quail-chicken hybrid. Specific immunoprecipitation of radiolabeled membrane proteins followed by electrophoretic separation and autoradiography w...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00499476
更新日期:1984-10-01 00:00:00
abstract::A bioautographic procedure has been developed for the visualization of the isozymes of dihydrofolate reductase (DHFR, E.C. 1.5.1.3). In addition to detecting electrophoretically separated enzymes, bioautography was utilized to visualize DHFR after isoelectric focusing on polyacrylamide gels. Both zone electrophoresis ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00504369
更新日期:1980-02-01 00:00:00
abstract::Because alternative RNA splicing regulation in the testis is prevalent, we explored testes of Sprague-Dawley rats for existence of alternatively spliced colony-stimulating factor 1 receptor (CSF-1R) mRNA. Using RT-PCR and sequencing, we identified a variant of CSF-1R mRNA that was 284 bp shorter than the full-length C...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-014-9649-6
更新日期:2014-06-01 00:00:00
abstract::Fourteen myoglobins of known sequence were examined by isoelectric focusing with and without urea. The 14 sequences formed six distinct mobility classes on gels without urea and three classes on those with urea. For these proteins, isoelectric focusing provides no advantage over single, nonequilibrium, nondenaturing g...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00504335
更新日期:1986-08-01 00:00:00
abstract::Potassium (K+) transport and channel systems play vital roles in plant growth, development and responses to various stresses. In this study, 44 putative K+-transport-related genes (18K+ uptake permease (KUP)/high-affinity K+ (HAK)/K+ transporter (KT) family genes and 26 channel genes, including 18 Shaker family genes ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-020-10004-z
更新日期:2020-09-29 00:00:00
abstract::The murine polycystic kidney disease gene, pcy, is an autosomal recessive trait located on chromosome 9. To determine the genetic locus of pcy, 222 intraspecific backcross mice were obtained by mating C57BL/6FG-pcy and Mus molossinus. Restriction fragment length polymorphism analysis of 70 of the 222 backcross progeny...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00554598
更新日期:1995-12-01 00:00:00
abstract::The alpha-amylase in Drosophila melanogaster is a highly polymorphic enzyme, at both the allozyme level and the specific activity level. This enzyme changes its specific activity drastically depending on both food conditions and developmental stages, and it has been suggested that the ability to change its activity de...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/a:1018715528413
更新日期:1999-12-01 00:00:00
abstract::Imprinted genes are characterized by monoallelic expression that is dependent on parental origin. Comparative analysis of imprinted genes between species is a powerful tool for understanding the biological significance of genomic imprinting. The slc38a4 gene encodes a neutral amino acid transporter and is identified a...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-018-9866-5
更新日期:2018-12-01 00:00:00
abstract::The genetic linkage relationships of the human glycosphingolipid beta-galactosidases were determined using human--mouse somatic cell hybrids. A new method was devised for the estimation of human galactosylceramide, lactosylceramide, and GMI-ganglioside beta-galactosidase activities in the presence of their mouse count...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484498
更新日期:1977-12-01 00:00:00
abstract::Congenital heart disease is the most common congenital anomaly, representing an important cause of infant morbidity and mortality. Congenital heart disease represents a group of heart anomalies that include septal defects, valve defects, and outflow tract anomalies. The exact genetic, epigenetic, or environmental basi...
journal_title:Biochemical genetics
pub_type: 杂志文章,评审
doi:10.1007/s10528-016-9780-7
更新日期:2017-04-01 00:00:00
abstract::Fifty-six sequences encoding the pina protein were characterized from three species or subspecies of einkorn wheat. These sequences contained 1,595 nucleotides, including 1,270 conserved sites, 21 single nucleotide polymorphisms (SNPs), and 16 indels. The average frequency of SNPs and indels was one out of 76.1 and 99...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-009-9239-1
更新日期:2009-06-01 00:00:00
abstract::Markhors (Capra falconeri) are among the most endangered mammal species, and several conservation measures, including ex situ breeding, are implemented to prevent their extinction. We studied sequence diversity and differentiation of the first hypervariable segment of the mitochondrial DNA control region among C. f. h...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-008-9145-y
更新日期:2008-04-01 00:00:00
abstract::Evidence is presented to show that the Sh locus specifies sucrose synthetase in the developing endosperm of maize. The sh/sh/sh endosperm possesses less than 10% sucrose synthetase activity as compared to the normal Sh/sh/sh endosperm. The residual enzyme activity in five independently derived mutant genotypes is attr...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00485135
更新日期:1976-12-01 00:00:00
abstract::Species in the genus Oryza (Poaceae) contain 10 genomic types and are distributed in pan-tropics of the world. To explore phylogenetic relationships of Oryza species having the AA-genome, DNA sequences of the chloroplast trnL intron and trnL-trnF spacer, mitochondrial nad1 intron 2, and nuclear internal transcribed sp...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-006-9062-x
更新日期:2007-02-01 00:00:00
abstract::Random amplified polymorphic DNA was used to assess the level of genetic diversity and genetic structure of Stipa krylovii (Gramineae), an important dominant species in the northern grasslands of China. Genetic diversity was low within S. krylovii populations, and diversity at the population level was associated with ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-006-9054-x
更新日期:2006-12-01 00:00:00
abstract::Myocardial infarction (MI) is common in India and the disease occurs at a relatively younger age. We wanted to look for association of Angiotensin I-converting enzyme (ACE) gene with MI in North East India. We also wanted to examine possible environmental interaction of ACE gene with established cardiovascular risk fa...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-015-9706-9
更新日期:2016-04-01 00:00:00
abstract::Basal activity of submaxillary gland (SMG) renin is high in female mice that carry the Rnrs allele and is induced to higher levels by treatment with dihydrotestosterone (DHT). To determine whether the difference in basal activity between high (Rnrs/Rnrs) and low (Rnrb/Rnrb) strains is due to enhanced sensitivity of Rn...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484623
更新日期:1981-06-01 00:00:00
abstract::Chronic myeloid leukemia (CML) is a hematopoietic stem cell malignancy characterized by the expression of the BCR-ABL1 fusion gene with different chimeric transcripts. Despite the crucial impact of constitutively active tyrosine kinase in CML pathogenesis, aberrant DNA methylation of certain genes plays an important r...
journal_title:Biochemical genetics
pub_type: 杂志文章,评审
doi:10.1007/s10528-018-9841-1
更新日期:2018-06-01 00:00:00
abstract::Patterns of lactate dehydrogenase (LDH) isozymes of tissues from various mouse strains were examined. An interstrain polymorphism for LDH isozymes of liver was established. One phenotype (CBA/Lac and AKR/J mice) yielded a five-banded LDH pattern, another phenotype (DBA/1J, DBA/2J, C57BL/6J and C3H/He) showed a three-b...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00500133
更新日期:1980-10-01 00:00:00
abstract::A null mutation at the phosphoglucomutase locus (Pgm-1) was discovered by electrophoretic analysis of the inbred mouse strain C57BL/6J. The null allele (Pgm-1n) was shown to segregate as a Mendelian unit alternative to the Pgm-1a and Pgm-1b alleles. Mice expressing the Pgm-1n allele, either in the heterozygous or homo...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484629
更新日期:1981-06-01 00:00:00
abstract::Electrophoretic variation is described for malic enzyme (ME) for the first time in brook trout (Salvelinus fontinalis). Since the quaternary structure of ME was not clear from examination of banding patterns in brook trout alone, ME phenotypes in rainbow trout (Salmo gairdneri)X brook trout hybrids as wel as in esoci...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00502121
更新日期:1979-08-01 00:00:00
abstract::Phosphoglucomutase (PGM) of adult stage in Drosophilia melanogaster has been characterized by gel filtration, ion-exchange chromatography, and isoelectric focusing. The two common electrophoretic variants, PGMA and PGMB, differ with respect to their kinetic and stability parameters. PGMA is more thermostable than PGMB...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00504306
更新日期:1979-10-01 00:00:00
abstract::Preeclampsia leads to maternal and perinatal morbidity and mortality. The literature in English was reviewed to summarize recent advances in understanding the global gene expression changes in the pathogenesis of preeclampsia. We identified at least eight consistently enriched categories, relating to pregnancy mainten...
journal_title:Biochemical genetics
pub_type: 杂志文章,评审
doi:10.1007/s10528-015-9684-y
更新日期:2015-10-01 00:00:00
abstract::To elucidate the phylogeny of the genus Paramesotriton (Caudata: Salamandridae), we investigated three mitochondrial DNA gene fragments (1207 bp in total) of cytochrome b, ND2, and ND4 for its six recognized species. The phylogenetic relationships within Paramesotriton were reconstructed by maximum parsimony (MP) and ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/b:bigi.0000026630.78770.fb
更新日期:2004-06-01 00:00:00
abstract::Site-specific gene modifications in cells are initiated by the introduction of exogenous DNA. We used a recently established cell assay to compare the ability of DNA donors to induce a single point mutation that converts a target gene encoding blue fluorescent protein (BFP) into expressing green fluorescent protein (G...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-010-9370-z
更新日期:2010-12-01 00:00:00
abstract::Scirpophaga incertulas, commonly referred to as yellow stem borer, is a predominant pest of rice causing serious losses in its yield. Genetic variation among populations of Scirpophaga incertulas collected from 28 hotspot locations in India was examined using the randomly amplified polymorphic DNA-polymerase chain rea...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/a:1002797219182
更新日期:2001-02-01 00:00:00
abstract::Genetic variation and differentiation of 12 populations of Picea jezoensis from the Russian Far East were studied using 20 allozyme loci. The mean number of alleles per locus was 2.63, the percent of polymorphic loci was 88.1%, the observed heterozygosity was 0.181, and the mean value of expected heterozygosity amount...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-006-9075-5
更新日期:2007-04-01 00:00:00
abstract::Polysomes from 5.5-day and 6.5-day embryonic chick erythroid cells contain messenger RNA (mRNA) which can be translated into products in a cell-free protein-synthesizing system. The products of the cell-free system coelectrophoresed with carrier globin chains from the cells from which the polysomal pellet was isolated...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00483979
更新日期:1977-10-01 00:00:00
abstract::Animal melanin has an important role in the formation of animal fur and skin, which is determined by its quantities, character, and distribution. To identify the effect of melanin on the formation of multi-colored Rex rabbits (Black, Chinchilla, Beaver, Protein cyan, Protein yellow, White), the structure of hair folli...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-019-09929-x
更新日期:2019-10-01 00:00:00
abstract::Ubiquitin-specific protease 9, Y-linked (USP9Y), is a protein encoded by the Y chromosome. Its precise function in the cell is unknown, although a role in the regulation of protein turnover has been postulated. Nonetheless, mutations in this gene could result in the over- or under-abundance of proteins involved in the...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-007-9139-1
更新日期:2008-04-01 00:00:00