Abstract:
:Potassium (K+) transport and channel systems play vital roles in plant growth, development and responses to various stresses. In this study, 44 putative K+-transport-related genes (18K+ uptake permease (KUP)/high-affinity K+ (HAK)/K+ transporter (KT) family genes and 26 channel genes, including 18 Shaker family genes and 8K+ channel outward (KCO) family genes) were identified in the genome of Chinese cabbage (Brassica rapa ssp. pekinensis). To clarify the molecular evolution of each family in Chinese cabbage, phylogenetic analysis and assessments of the gene structures, conserved motifs, chromosomal locations, gene duplications, expression patterns and cis-acting elements of the 44 putative K+-transport-related genes were performed. The phylogenetic analysis showed that these genes could be classified into five clades [KUP/HAK/KTs, KCOs, Kout, Kin (KAT) and Kin (AKT)] and that the members of a given clade shared conserved exon-intron distributions and motif compositions. These K+-transport-related genes were unevenly distributed over all ten chromosomes, including four duplicated gene pairs that implied an expansion of K+-transport-related genes in Chinese cabbage. Analyses of Illumina RNA-seq data for these 44K+-transport-related genes indicated tissue-/organ-specific expression patterns. In addition, an overall evaluation showed that the expression levels of KUP/HAK/KT genes were significantly higher than those of potassium channel genes in six tissues. Promoter cis-acting element analysis revealed that these 44K+-transport-related genes may be associated with responses to 10 abiotic stresses, primarily light, methyl jasmonate (MeJA) and abscisic acid (ABA). Our results provide a systematic and comprehensive overview of K+-transport-related gene families in Chinese cabbage and establish a foundation for further research on K+ absorption and transport functions.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Shen C,Yuan Jdoi
10.1007/s10528-020-10004-zsubject
Has Abstractpub_date
2020-09-29 00:00:00eissn
0006-2928issn
1573-4927pii
10.1007/s10528-020-10004-zpub_type
杂志文章abstract::An enzyme catalyzing the transfer of the glucosyl moiety of UDP-glucose to the 3-hydroxyl group of cyanidin has been demonstrated in petal extracts of Silene dioica mutants with cyanidin-3-O-glucoside in the petals. This transferase activity was also present in young rosette leaves and calyces of these plants. The hig...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484525
更新日期:1978-12-01 00:00:00
abstract::We extracted the yellow melanin (phaeomelanin), black melanin (eumelanin), and mixed type of melanin from dorsal hair of dominant yellow (Ay/a), non-agouti (a/a), and agouti (A/A) mice, respectively. Spectrophotometric and fluorescence spectrophotometric analysis demonstrated that the yellow melanin was qualitatively ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484724
更新日期:1978-08-01 00:00:00
abstract::ZFY-like genes have been observed in a variety of vertebrate species. Although originally implicated as the primary testis-determining gene in humans and other placental mammals, more recent evidence indicates a role(s) outside that of testis determination. In this study, DNA from five species of fish, Carasius auratu...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::The single-strand conformation polymorphism (SSCP) procedure has been applied in routine testing for hereditary diseases. Temperature, running buffer, gel composition, and fragment length can influence its sensitivity. Mutation detection in the clinical setting depends on the development of automated technology, espec...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-007-9109-7
更新日期:2007-10-01 00:00:00
abstract::In this study, we identified porcine single nucleotide polymorphisms (SNPs) by aligning eight sequences generated with two approaches: amplification of 665 intronic regions using one sample from each of eight breeds, including three East Asian pigs, and amplification of 289 3'-UTR regions using two samples from each o...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-011-9486-9
更新日期:2012-06-01 00:00:00
abstract::Fifty-six sequences encoding the pina protein were characterized from three species or subspecies of einkorn wheat. These sequences contained 1,595 nucleotides, including 1,270 conserved sites, 21 single nucleotide polymorphisms (SNPs), and 16 indels. The average frequency of SNPs and indels was one out of 76.1 and 99...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-009-9239-1
更新日期:2009-06-01 00:00:00
abstract::The locus for sorbitol dehydrogenase (SORD, E.C. 1.1.1.14) has been shown to segregate with hexosaminidase A and mannose phosphate isomerase in a series of human-Chinese hamster somatic cell hybrids. Cytogenetic analysis supports the assignment to chromosome 15 and further defines the gene locus to the region 15pter l...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484391
更新日期:1980-06-01 00:00:00
abstract::Studies on hybridization, inheritance, and population genetics of brown planthoppers that infest rice and weeds were undertaken using starch gel electrophoresis to determine whether the weed-infesting population represents a biological race or a species. F(1) and F(2) generations were produced by crosses between paren...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-009-9316-5
更新日期:2010-04-01 00:00:00
abstract::Larval serum protein-1 (LSP-1) and LSP-2 are the major proteins of Drosophila larval serum. The amount of LSP-1 synthesized is strictly proportional to the number of LSP-1 genes present within the range 1-10. The normal number in female flies is 6. Flies with extreme amounts of LSP-1 were, by our criteria, as fit as t...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00499473
更新日期:1984-10-01 00:00:00
abstract::It is shown that the gene controlling the synthesis of the organ-specific S-esterase of Drosophila virilis ejaculatory bulbs is located on the second chromosome (at approximate position 192.1 +/- map units). The cells of the genital imaginal disks are determined for the synthesis of S-esterase 10-12 hr after the secon...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484728
更新日期:1978-08-01 00:00:00
abstract::Hippophae rhamnoides ssp. sinensis is endemic to China, and it is a dioecious, outcrossing plant. Although many studies have been undertaken mainly on its agricultural, nutritional, medical, and ornamental value, little is known about its population genetics. This study uses random amplified polymorphic DNA to investi...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-006-9025-2
更新日期:2006-06-01 00:00:00
abstract::The primary structures of the alpha globins from CE/J, DBA/2J, and a stock of Potter's mice were determined to identify the amino acid substitutions associated with the unique isoelectric focusing patterns of these hemoglobins. In addition, the primary structures of the alpha globins from MOL III and PERU mice were st...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484946
更新日期:1982-02-01 00:00:00
abstract::Differences in Zn-induced levels of hepatic metallothionein (MT) in inbred strains of the mouse are described. Three low-producing strains, C57 BL/6, C57BL/10, and NIH, are identified, while C3H and CBA display the highest levels of hepatic MT following Zn treatment. These interstrain differences affect not only the l...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF02401801
更新日期:1989-04-01 00:00:00
abstract::Polymorphism of the mtDNA ND5 gene was detected by PCR-SSCP and DNA sequencing methods in 714 individuals from six Chinese cattle breeds. The results showed that at the P2 locus, two kinds of haplotypes, named A and B, with three SNPs (T12900C, A12923T, C12924T) were detected. The frequencies of haplotype A in six bre...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-008-9152-z
更新日期:2008-06-01 00:00:00
abstract::During a large-scale screen of a human fetal brain cDNA library, a full-length cDNA encoding a novel Rap2 interacting protein was isolated and sequenced. The cDNA is 3397bp long and has a predicted open reading frame encoding a protein of 329 aa. The predicted protein shows high homology to mouse and human RPIP8, and ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/a:1020918411532
更新日期:2003-02-01 00:00:00
abstract::A major category of mutant hematopoietic phenotypes in Drosophila is melanotic tumors or nodules, which consist of abnormal and overproliferated blood cells, similar to granulomas. Our analyses of the melanotic mutant dappled have revealed a novel type of gene involved in blood cell regulation. The dappled gene is an ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-010-9411-7
更新日期:2011-06-01 00:00:00
abstract::Restriction endonuclease analyses of DNA from one Black G gamma A gamma-HPFH homozygote and four Black and one Indian G gamma A gamma-HPFH heterozygotes have identified three different HPFH types which are the result of large deletions including the delta and beta genes. Two of the types are comparable to those charac...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00499284
更新日期:1984-02-01 00:00:00
abstract::Considering that the genetically modified soybean lectin gene is affected by the gene type, to improve the stability of the genetically modified soybean lectin gene, a method based on fluorescence quantitative PCR to analyze the genetic characteristics of the genetically modified soybean lectin was proposed. The commo...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-020-09998-3
更新日期:2020-09-26 00:00:00
abstract::The molecular weight forms of kynurenine formamidase were studied both gentically and biochemically. Formamidase I (native molecular weight 60,000) was purified using (NH4)2SO4 and pH fractionation, DEAE-cellulose chromatography at two different pH's, hydroxylapatite chromatography, and Sephadex G-100 gel filtration. ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484718
更新日期:1978-08-01 00:00:00
abstract::Arylsulfatase A (ASA) activity was studied from five blood fractions, leukocytes plus platelets (LKPL), leukocytes (LK), neutrophils (N), lymphocytes (LYM), and platelets (PL). No significant difference was found among the mean ASA specific activity values for the fractions. Electrophoretic examination revealed four d...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484068
更新日期:1982-10-01 00:00:00
abstract::Two types of alleles exist in the human alcohol dehydrogenase-2 (ADH2) locus. The usual ADH12 allele is common in Caucasians, while the atypical ADH22 allele is predominant in Orientals. The ADH22 produces the beta 2 subunit, which is catalytically far more active than the beta 1 subunit produced by the ADH12 gene. Th...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF02399419
更新日期:1988-08-01 00:00:00
abstract::The concentration of D-beta-aminoisobutyric acid (D-BAIB) in the liver and kidney was twice as high and dropped more slowly in the female mouse than in the male after an intraperitoneal injection of thymine. The concentration of beta-alanine, formed from uracil by the same enzyme system catalyzing formation of D-BAIB ...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00502136
更新日期:1979-08-01 00:00:00
abstract::The soluble proteins of the normal and male-sterile stamenless-2 (sl-2/sl-2) mutant of tomato (Lycopersicon esculentum) grown in different temperatures were analyzed by one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The normal and mutant stamens had some common proteins, but certai...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00556214
更新日期:1987-10-01 00:00:00
abstract::CCR5 is a seven-transmembrane G-protein-coupled receptor that binds the CC-chemokines including RANTES, eotaxin, MIP-1alpha and beta. CCR5 serves as an essential coreceptor for cell entry of R5 (macrophage-tropic, nonsyncytium-inducing) strains of HIV-1. To date, four deletions have been found in human and primate ccr...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:
更新日期:2005-06-01 00:00:00
abstract::A method for detecting two alleles at Np-1 (nucleoside phosphorylase) and three alleles at Es-10 (esterase 10) from mouse blood by cellulose acetate electrophoresis is described. The allelic constitution at these loci for 44 inbred strains and stocks was determined. The location of Np-1 on chromosome 14 was establishe...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484465
更新日期:1977-04-01 00:00:00
abstract::The rice cultivar Hikarishinseiki, a semidwarf isogenotype of Koshihikari carrying the Green Revolution sd1 gene, is increasingly grown in both Japan and the United States. Here, we report DNA diagnosis for Hikarishinseiki targeting its Jukkoku-type sd1 locus, which codes for a defective gibberellin 20-oxidase, with a...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/s10528-013-9584-y
更新日期:2013-08-01 00:00:00
abstract::A survey of 37 inbred strains for erythrocyte purine nucleoside phosphorylase activity showed a greater than threefold range. Six of these strains had significantly greater activity than the others, and all of the high-activity strains had the Np-2 electrophoretic band. The high-purine nucleoside phosphorylase activit...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00499142
更新日期:1983-04-01 00:00:00
abstract::Male and female wild Norway rats (Rattus norvegicus Erxleben) and male and female albino outbred rats (Ipf:RIZ) were crossbred. These animals were the control, noninbred group (0% inbred). By systematic full-sib mating, two experimental groups (50 and 91% of inbred) were raised. Half of each group (both males and fema...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00569326
更新日期:1992-08-01 00:00:00
abstract::Using megagametophyte (maternal haploid) and embryo (diploid) tissues of incense-cedar seeds, the expression of one of three malate dehydrogenase (MDH) loci was found to be influenced by a second, unlinked, modifier locus. Whereas alleles of the affected structural locus are codominant, the modifier alleles show domin...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1007/BF00484435
更新日期:1983-06-01 00:00:00
abstract::A novel cDNA fragment was identified from a human fetal brain cDNA library by using the coding sequence of human BRI3 gene (Accession No. NM015379) as bait in a yeast two-hybrid screening. Then by 5'-RACE (rapid amplification of cDNA end) and electronic hybridization, we obtained a 1.9 kb contig which consists of a no...
journal_title:Biochemical genetics
pub_type: 杂志文章
doi:10.1023/a:1013807518791
更新日期:2001-12-01 00:00:00