Comparative immunochemical studies of primate hemoglobins.

Abstract:

:The antigenic properties of a number of chromatographically purified primate hemoglobins were compared to those of normal human hemoglobin using a sensitive radioimmunochemical procedure. The degree of inhibition of the antigen-antibody reaction with heterologous hemoglobins appeared to be related to the structural similarity of these proteins to the normal human hemoglobin immunogen. With the exception of the baboon hemoglobin, the antigenicity of the hemoglobins paralleled the phylogeny of the primates. The gorilla and chimpanzee hemoglobins were antigenically identical to normal human hemoglobin, whereas the gibbon and orangutan hemoglobins were substantially more variable. Of the Old World monkey hemoglobins examined, the baboon produced lower inhibition values, suggesting a greater degree of structural dissimilarity than other Cercopithecoidea hemoglobins, which is compatible with a greater rate of evolutionary change occurring in this protein. Using the known amino acid sequences of human and other primate hemoglobins, we have attempted to identify antigenic determinant areas of the proteins.

journal_name

Biochem Genet

journal_title

Biochemical genetics

authors

Garver FA,Talmage DW

doi

10.1007/BF00484406

subject

Has Abstract

pub_date

1975-12-01 00:00:00

pages

743-57

issue

11-12

eissn

0006-2928

issn

1573-4927

journal_volume

13

pub_type

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