Abstract：
:The power of fluorescence microscopy to study cellular structures and macromolecular complexes spans a wide range of size scales, from studies of cell behavior and function in physiological 3D environments to understanding the molecular architecture of organelles. At each length scale, the challenge in 3D imaging is to extract the most spatial and temporal resolution possible while limiting photodamage/bleaching to living cells. Several advances in 3D fluorescence microscopy now offer higher resolution, improved speed, and reduced photobleaching relative to traditional point-scanning microscopy methods. We discuss a few specific microscopy modalities that we believe will be particularly advantageous in imaging cells and subcellular structures in physiologically relevant 3D environments.
journal_name
Trends Cell Bioljournal_title
Trends in cell biologyauthors
Fischer RS,Wu Y,Kanchanawong P,Shroff H,Waterman CMdoi
10.1016/j.tcb.2011.09.008subject
Has Abstractpub_date
2011-12-01 00:00:00pages
682-91issue
12eissn
0962-8924issn
1879-3088pii
S0962-8924(11)00198-Xjournal_volume
21pub_type
杂志文章,评审abstract：:Virtually all cancers show metabolic changes that result in upregulation of glycolysis and glucose consumption. Although discovered in the 1920s, how this glycolytic switch happens, and whether it is a cause or a consequence of the malignant process, has remained a matter of debate. The p53 tumor suppressor gene, disc...
journal_title：Trends in cell biology
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abstract：:Ciliary movement is powered by axonemal dynein. This article considers how a signal transduction cascade initiated at the cell membrane may activate outer dynein arms to change the velocity of microtubule sliding and the swimming speed of ciliated cells. For Paramecium, a critical event in the cascade is the cAMP-depe...
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journal_title：Trends in cell biology
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abstract：:The basement membrane (BM), a specialized network of extracellular matrix macromolecules, surrounds epithelial, endothelial, muscle, fat and nerve cells. During development, immune surveillance and disease states ranging from cancer to fibrosis, host cells penetrate the BM by engaging tissue-invasive programs, the ide...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
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abstract：:It is well established that the target of rapamycin (TOR) protein kinase has pivotal roles in controlling cell functions (including protein synthesis, cell growth and cell proliferation) and is implicated in numerous human diseases. Mammalian TOR complex 1 (mTORC1) signalling is activated by hormones and growth factor...
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journal_title：Trends in cell biology
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journal_title：Trends in cell biology
pub_type： 杂志文章,评审
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journal_title：Trends in cell biology
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journal_title：Trends in cell biology
pub_type： 杂志文章,评审
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journal_title：Trends in cell biology
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journal_title：Trends in cell biology
pub_type： 杂志文章,评审
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journal_title：Trends in cell biology
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journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0962-8924(01)02173-0
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journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2016.08.004
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journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2016.05.003
更新日期：2016-09-01 00:00:00
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journal_title：Trends in cell biology
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journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2016.12.001
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journal_title：Trends in cell biology
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journal_title：Trends in cell biology
pub_type： 杂志文章,评审
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journal_title：Trends in cell biology
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journal_title：Trends in cell biology
pub_type： 杂志文章,评审
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journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2007.04.002
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journal_title：Trends in cell biology
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journal_title：Trends in cell biology
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journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/0962-8924(96)10028-3
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journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2009.09.009
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abstract：:Mitochondria possess a complex architecture with two membranes. The inner membrane is divided into two domains: the inner boundary membrane, which is adjacent to the outer membrane, and membrane invaginations termed cristae. Both domains are connected by tubular openings, the crista junctions. Recent studies led to th...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/j.tcb.2012.01.004
更新日期：2012-04-01 00:00:00
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journal_title：Trends in cell biology
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abstract：:The role o f iron in cell division, cell death and human disease has recently gained increased attention. The best studied process for iron uptake into mammalian cells involves traps ferrin and its receptor. This review discusses evidence supporting the existence of other routes by which iron can enter mammalian cells...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/0962-8924(96)10019-2
更新日期：1996-06-01 00:00:00
