Abstract：
:Cellular membranes differ in their molecular organisation, shape, and dynamics. Knowing how these properties of membrane architecture relate to the presence and function of specific membrane components is fundamental for understanding membrane-associated cellular processes. Correlative light and electron microscopy (CLEM) is ideally poised to address such problems. Fluorescence microscopy allows identification of cellular membranes through labelled components and can provide temporal information, while electron microscopy allows visualisation of the structure of the same membranes at high resolution. In recent years, various CLEM protocols have been applied to gain insights into cellular membrane architecture. Here, we review conceptually novel approaches by which CLEM has provided insights on membrane reshaping, subcellular localisation of components, host-pathogen interactions, and has answered longstanding mechanistic questions.
journal_name
Trends Cell Bioljournal_title
Trends in cell biologyauthors
Ganeva I,Kukulski Wdoi
10.1016/j.tcb.2020.04.003subject
Has Abstractpub_date
2020-07-01 00:00:00pages
577-587issue
7eissn
0962-8924issn
1879-3088pii
S0962-8924(20)30074-Xjournal_volume
30pub_type
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