Abstract:
:Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-targeting T-cells from RRMS patients and controls. CDR3 region analysis of TCRβ chains revealed biased use of specific TCRBV-bearing CD4+ clones. CD8+ clones showed homology to published TCR from CNS-infiltrating T-cells in MS lesions. These studies are the first description of TCR usage of CNS-specific CD8+ T-cells and provide insights into their potential regulatory role in disease.
journal_name
J Neuroimmunoljournal_title
Journal of neuroimmunologyauthors
Biegler BW,Yan SX,Ortega SB,Tennakoon DK,Racke MK,Karandikar NJdoi
10.1016/j.jneuroim.2011.02.001subject
Has Abstractpub_date
2011-05-01 00:00:00pages
131-40issue
1-2eissn
0165-5728issn
1872-8421pii
S0165-5728(11)00025-7journal_volume
234pub_type
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