Abstract：
:Flow visualization using fluorescent microparticles and cell viability investigations are carried out to examine the mechanisms by which cells are seeded into scaffolds driven by surface acoustic waves. The former consists of observing both the external flow prior to the entry of the suspension into the scaffold and the internal flow within the scaffold pores. The latter involves micro-CT (computed tomography) scans of the particle distributions within the seeded scaffolds and visual and quantitative methods to examine the morphology and proliferation ability of the irradiated cells. The results of these investigations elucidate the mechanisms by which particles are seeded, and hence provide valuable information that form the basis for optimizing this recently discovered method for rapid, efficient, and uniform scaffold cell seeding. Yeast cells are observed to maintain their size and morphology as well as their proliferation ability over 14 days after they are irradiated. The mammalian primary osteoblast cells tested also show little difference in their viability when exposed to the surface acoustic wave irradiation compared to a control set. Together, these provide initial feasibility results that demonstrate the surface acoustic wave technology as a viable seeding method without risk of denaturing the cells.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Bok M,Li H,Yeo LY,Friend JRdoi
10.1002/bit.22243subject
Has Abstractpub_date
2009-06-01 00:00:00pages
387-401issue
2eissn
0006-3592issn
1097-0290journal_volume
103pub_type
杂志文章abstract：:The removal of substate molecules from aerobic microbial cultures is due to both consumption by microorganisms and stripping by the air stream. The air stripping component can be described by a constant parameter for low concentrations of volatile substrates. This air stripping parameter was found to have a value of 0...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260300409
更新日期：1987-09-01 00:00:00
abstract：:Short-chain carboxylic acids generated by various mixed- or pure-culture fermentation processes have been considered valuable precursors for production of bioalcohols. While conversion of carboxylic acids into alcohols is routinely performed with catalytic hydrogenation or with strong chemical reducing agents, here, a...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.24786
更新日期：2013-04-01 00:00:00
abstract：:Anerobic biofilms with dominantly acidogenic bacteria were grown in fixed-bed recycle reactors. The influence of calcium concentration in the culture medium on biofilm mass accumulation, immobilized calcium concentration, and biofilm-specific activity was investigated. The results indicate that the biofilm mass accumu...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260450305
更新日期：1995-02-05 00:00:00
abstract：:In addition to the role of maintaining the pH, buffers can also facilitate the transport of H(+) ions in acid-generating systems. The role of this facilitation in proton transport in porous pellets on acid-generating immobilized enzymic reactions is examined. The activity in these systems can be maximized by a proper ...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260270609
更新日期：1985-06-01 00:00:00
abstract：:Acid phosphatase thermal deactivation follows a complex path: an initial decay toward an equilibrium distribution of at least two intermediate structures, mutually at the equilibrium, followed by a final breakdown toward a completely inactive enzyme configuration. The results obtained in the presence of sorbitol have ...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260381006
更新日期：1991-12-05 00:00:00
abstract：:To initiate an infection human immunodeficiency virus type 1 (HIV-1) particles must first bind to receptors on the surface of their host cells, a process that eventually leads to fusion of viral and cellular membranes and release of the viral genome into the cytoplasm. Understanding the molecular mechanisms of these p...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.20680
更新日期：2006-02-05 00:00:00
abstract：:A gene for ethionine resistance isolated from the yeast Saccharomyces cerevisiae DKD-5D-H conferred on the yeast cells resistance to seleno-L-methionine and capability to produce S-adenosyl-L-methionine in the cells. An enzymatic study of the L-methionine synthetic pathway of L-methionine proto- and auxotrophs and in ...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260351107
更新日期：1990-05-01 00:00:00
abstract：:This article develops and utilizes an in situ technique to estimate the Monod half-maximum rate concentration, K(s) and the maximum specific utilization rate constant, k, for biofilms. The technique employs a curve-matching method with kinetic results from several short-term experiments with completely mixed biofilm r...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260281120
更新日期：1986-11-01 00:00:00
abstract：:The feasibility of continuous ester synthesis in a membrane bioreactor (MBR) by a recombinant cutinase from Fusarium solani pisi was investigated, using the optimal conditions previously attained by medium engineering. The objective was to analyze the MBR behavior as a differential or an integral reactor. The main com...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/1097-0290(20010120)72:2<127::aid-bit1>3.0.
更新日期：2001-01-20 00:00:00
abstract：:Hydrogen production by Rhodobacter capsulatus is an anaerobic, photobiological process requiring specific mixing conditions. In this study, an innovative design of a photobioreactor is proposed. The design is based on a plate-type photobioreactor with an interconnected meandering channel to allow culture mixing and H2...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.27656
更新日期：2020-12-15 00:00:00
abstract：:Animal-derived serum is an essential media supplement for mammalian cells in cell culture. For a number of reasons including cost, regulatory concerns, lot inconsistency, potential contamination with adventitious agents, and down-stream processing it is desirable to eliminate the use of serum. Existing protocols desig...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.21707
更新日期：2008-04-15 00:00:00
abstract：:Genome-scale flux analysis of Escherichia coli DH5alpha growth in a complex medium was performed to investigate the relationship between the uptake of various nutrients and their metabolic outcomes. During the exponential growth phase, we observed a sequential consumption order of serine, aspartate and glutamate in th...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.22119
更新日期：2009-02-15 00:00:00
abstract：:Capitalizing on components evolved to metabolize ethanol in Aspergillus nidulans, we previously designed the first molecular gas-gene expression interface using gaseous acetaldehyde as the major inducer. This fungus-derived acetaldehyde-inducible gene regulation (AIR) system operated perfectly and enabled precise and ...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.20482
更新日期：2005-06-30 00:00:00
abstract：:In this work, novel conductive composite nanofiber mesh possessing reactive groups was electrospun from solutions containing poly(acrylonitrile-co-acrylic acid) (PANCAA) and multi-walled carbon nanotubes (MWCNTs) for redoxase immobilization, assuming that the incorporated MWCNTs could behave as electrons transferor du...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.21280
更新日期：2007-07-01 00:00:00
abstract：:The thermophilic fungus Sporotrichum cellulophilum was immobilized with nonwoven materials for cellulase production. The cellulose powder concentration in the medium was an important factor controlling cellulase production. When the cellulose powder concentration in the nonwoven materials was more than 4%, cellulase p...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260280814
更新日期：1986-08-01 00:00:00
abstract：:Chlorella sp. strain VJ79 was isolated from a total heterotrophic count of a wastewater collector. It grows autotrophically, heterotrophically, and mixotrophically on a variety of organic substrates. Glucose and serine promote a mixotrophic growth from which the yield is higher than the sum of autotrophic and heterotr...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260260707
更新日期：1984-07-01 00:00:00
abstract：:Analytical expressions describing convective flow in a continuous arteriovenous hollow fiber hemofilter were developed. In the lumen of the hollow fiber membrane, existing analytical expressions were applied to describe velocity profiles and pressure. For flow in the shell (the extracapillary space separating the fibe...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：
更新日期：2000-04-20 00:00:00
abstract：:A novel reaction system was developed to maximize the catalytic efficiency of chloroperoxidase (CPO, from Caldariomyces fumago) toward the oxidation of hydrocarbons. The reaction system consisted of an organic/aqueous emulsion comprising pure substrate and aqueous buffer supplemented with the surfactant dioctyl sulfos...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.20769
更新日期：2006-05-05 00:00:00
abstract：:Proteolysis associated with recombinant protein expression in Chinese Hamster Ovary (CHO) cells has hindered the development of biologics including HIV vaccines. When expressed in CHO cells, the recombinant HIV envelope protein, gp120, undergoes proteolytic clipping by a serine protease at a key epitope recognized by ...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.27016
更新日期：2019-09-01 00:00:00
abstract：:A biosensor detecting estrogens, progestogens, and androgens in complex samples and in a single step is described. Three Arxula adeninivorans yeast strains were created, each strain producing a different recombinant human hormone receptor and a different fluorescent reporter protein. These strains were then mixed to c...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.26249
更新日期：2017-07-01 00:00:00
abstract：:A limited life cycle assessment (LCA) was performed on a combined biological and chemical process for the production of adipic acid, which was compared to the traditional petrochemical process. The LCA comprises the biological conversion of the aromatic feedstocks benzoic acid, impure aromatics, toluene, or phenol fro...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.23074
更新日期：2011-06-01 00:00:00
abstract：:The solution structure of plasmid DNA is known to be a strong function of solution conditions due to intramolecular electrostatic interactions between the charged phosphate groups along the DNA backbone. The objective of this work was to determine whether it was possible to enhance the use of ultrafiltration for separ...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.25836
更新日期：2016-04-01 00:00:00
abstract：:One of the major bottlenecks in the bioconversion of lignocelluosic feedstocks to liquid ethanol is the recalcitrance of residue following pretreatment, specifically softwood derived residues. Peroxide delignification has previously been shown to effectively aid in the removal of condensed lignaceous moieties from sub...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.22768
更新日期：2010-08-15 00:00:00
abstract：:The dissociation constant, K(d) , is an important parameter for characterizing protein-protein interaction affinities. SUMOylation is one of the important protein post-translational modifications and it involves a multi-step enzymatic cascade reaction, resulting in peptide activation and substrate conjugation. Multipl...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.24564
更新日期：2012-11-01 00:00:00
abstract：:A chimeric Fab was expressed in Chinese hamster ovary cells under the control of the CMV promoter in a two-stage production process. Cells were first grown to 90% confluence at 37 degrees C in a proliferation phase, followed by a production phase at either 37 degrees C or 28 degrees C. Medium supplemented with serum a...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.10793
更新日期：2003-11-20 00:00:00
abstract：:In this study, a non-sterile (open) continuous fermentation (OCF) process with no-carbon loss was developed to improve lactic acid (LA) productivity and operational stability from the co-utilization of lignocellulose-derived sugars by thermophilic Enterococcus faecium QU 50. The effects of different sugar mixtures on ...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.27313
更新日期：2020-06-01 00:00:00
abstract：:The effect of the water content on the lipase-catalyzed (Candida rugosa) interesterification (acidolysis) of menhaden oil with conjugated linoleic acid was studied for amounts of added water ranging from 0-4% (w/w). The rate of the acidolysis reaction increased with increasing water content, but the corresponding perc...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.10223
更新日期：2002-06-05 00:00:00
abstract：:This research focused on how the extent and type of primary solid-liquid separation can affect the performance of guard filtration and chromatography, in this instance hydrophobic interaction chromatography. The system used in the study was yeast (Saccharomyces cerevisiae) with the target molecule being an intracellul...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.21823
更新日期：2008-08-01 00:00:00
abstract：:A fuzzy-logic light attenuation model was developed and validated for a stirred-tank photobioreactor. Based on this model, local light intensities were used to calculate local specific growth rates of the cyanobacteria Synechococcus PCC 7942. The light regime for maximization of biomass space-time yield in a batch pro...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.21086
更新日期：2006-12-20 00:00:00
abstract：:Enzymatic hydrolysis of cellulose for sugar production offers advantages of higher conversion, minimal by-product formation, low energy requirements, and mild operating conditions over other chemical conversions. The development of a kinetic model, based on observable, macroscopic properties of the overall system, is ...
journal_title：Biotechnology and bioengineering
pub_type： 杂志文章
doi：10.1002/bit.260260305
更新日期：1984-03-01 00:00:00
