Bioartificial kidney. I. Theoretical analysis of convective flow in hollow fiber modules: application to a bioartificial hemofilter.


:Analytical expressions describing convective flow in a continuous arteriovenous hollow fiber hemofilter were developed. In the lumen of the hollow fiber membrane, existing analytical expressions were applied to describe velocity profiles and pressure. For flow in the shell (the extracapillary space separating the fibers), analytical expressions for the radial and axial velocity profiles and pressure distribution were derived by first finding the stream function. The expressions are based on a similarity solution. Previous analyses of ultrafiltration have either ignored osmotic pressure or assumed constant shell pressure. In this paper, the axial variation in lumen pressure, shell pressure, and osmotic pressure were accounted for. The predicted filtration rates agree well with the experimental results. This flow model is general enough to describe flow in hollow fiber membrane systems employed as bioreactors (e.g., for cell cultures and as bioartificial organs) and as separators (e.g., ultrafiltration and microfiltration) operating in the open-shell mode. The results were applied to determine the design of an optimally functioning bioartificial hemofilter for use ex vivo or in vivo.


Biotechnol Bioeng


Moussy Y


Has Abstract


2000-04-20 00:00:00














  • Dynamic simulator for anaerobic digestion processes.

    abstract::A transient, two-culture model simulating methane production from biomass has been developed. The simulator, based partially on the work by Andrews and McCarty, is capable of calculating the hydrolysis products of several common organic materials, accommodating various substrate feeding modes, and simulating the trans...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kleinstreuer C,Poweigha T

    更新日期:1982-09-01 00:00:00

  • Biocatalytic polyester synthesis: analysis of the evolution of molecular weight and end group functionality.

    abstract::The enzymatic synthesis of polyesters from activated diesters and diols has been investigated. Differences between enzymatic synthesis and traditional chemical condensation processes are discussed. The disappearance of monomers during the initial phase of reaction indicates that enzyme has a higher specificity for tra...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chaudhary AK,Beckman EJ,Russell AJ

    更新日期:1997-07-05 00:00:00

  • The mechanism of uranium biosorption by Rhizopus arrhizus.

    abstract::Biosorption of elements is a little understood phenomenon exhibited by some types of even nonliving microbial biomass. A common fungus Rhizopus arrhizus has been reported to take up uranium from aqueous solutions to the extent of 180 mg U(6+)/g. The mechanism of uranium sequestering by this type of biomass was studied...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Tsezos M,Volesky B

    更新日期:1982-02-01 00:00:00

  • Biocatalytic process optimization based on mechanistic modeling of cholic acid oxidation with cofactor regeneration.

    abstract::Reduction and oxidation of steroids in the human gut are catalyzed by hydroxysteroid dehydrogenases of microorganisms. For the production of 12-ketochenodeoxycholic acid (12-Keto-CDCA) from cholic acid the biocatalytic application of the 12α-hydroxysteroid dehydrogenase of Clostridium group P, strain C 48-50 (HSDH) is...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Braun M,Link H,Liu L,Schmid RD,Weuster-Botz D

    更新日期:2011-06-01 00:00:00

  • Growth dynamics of a methylotroph (Methylomonas L3) in continuous cultures. II. Growth inhibition and comparison against an unstructured model.

    abstract::High methanol concentrations have a negative effect on the growth rate and the biomass yield of growth transients induced by methanol pulses in continuous cultures of Methylomonas L3. The physiological basis of this effect is investigated by measuring the effect of the methanol pulse on the cell energy charge (EC) and...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chu IM,Papoutsakis ET

    更新日期:1987-01-01 00:00:00

  • Mechanical load increase-induced changes in cytoskeletal structure and cellular barrier function in human cerebral endothelial cells.

    abstract::Globally, approximately a billion patients are estimated to suffer from neurological disorders. Although there are many therapeutic candidates for the central nervous system, treatment of brain disorders is restricted by the blood-brain barrier (BBB), which is a highly selective membrane that protects the brain from e...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kim D,Kwon S

    更新日期:2018-10-01 00:00:00

  • Biomass/adsorbent electrostatic interactions in expanded bed adsorption: a zeta potential study.

    abstract::Expanded bed adsorption is an integrated technology that allows the introduction of particle-containing feedstock without the risk of blocking the bed. The biomass particles contained in the feedstock have to be treated as an integral part of the process and potential interactions between suspended biomass and the ads...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Lin DQ,Brixius PJ,Hubbuch JJ,Thömmes J,Kula MR

    更新日期:2003-07-20 00:00:00

  • Countercurrent gradient chromatography: A continuous focusing technique.

    abstract::The continuous separation of proteins was performed in a countercurrent gradient chromatography (CGC) system. A magnetically stabilized fluidized bed (MSFB) was used to establish true countercurrent contact of a solid resin with a liquid buffer. STable pH gradients were formed in the system in less than 10 min and rem...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Evans LL,Burns MA

    更新日期:1995-12-05 00:00:00

  • Fluorescence-based sensing system for copper using genetically engineered living yeast cells.

    abstract::A whole cell-based optical sensing system for copper was developed based on Saccharomyces cerevisiae cells harboring plasmid pYEX-GFPuv. The basis of this system was the ability of the transcriptional activator protein Ace1 present in S. cerevisiae to control the expression of the reporter protein, GFPuv. When copper ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Shetty RS,Deo SK,Liu Y,Daunert S

    更新日期:2004-12-05 00:00:00

  • Fluorescent silica particles for monitoring oxygen levels in three-dimensional heterogeneous cellular structures.

    abstract::Bacterial biofilms are a major obstacle challenging the development of more effective therapies to treat implant infections. Oxygen availability to bacterial cells has been implicated in biofilm formation and planktonic cell detachment; however, there are insufficient tools available to measure oxygen concentrations w...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Acosta MA,Velasquez M,Williams K,Ross JM,Leach JB

    更新日期:2012-10-01 00:00:00

  • Comparative metabolite analysis to understand lactate metabolism shift in Chinese hamster ovary cell culture process.

    abstract::A metabolic shift from lactate production (LP) to net lactate consumption (LC) phenotype was observed in certain Chinese hamster ovary (CHO) cell lines during the implementation of a new chemically defined medium (CDM) formulation for antibody production. In addition, this metabolic shift typically leads to process pe...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Luo J,Vijayasankaran N,Autsen J,Santuray R,Hudson T,Amanullah A,Li F

    更新日期:2012-01-01 00:00:00

  • Dynamic behavior of adsorber membranes for protein recovery.

    abstract::In recent years there has been a considerable interest in developing membrane chromatography systems that function as a short, wide chromatographic column in which the adsorptive packing consists of one or more microporous membranes. This study reports the use of new adsorber membranes prepared by the incorporation of...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Avramescu ME,Borneman Z,Wessling M

    更新日期:2003-12-05 00:00:00

  • N-removal in a granular sludge sequencing batch airlift reactor.

    abstract::The removal of N-compounds in the sequencing batch airlift reactor (SBAR) containing granular sludge was studied under conditions of decreased dissolved oxygen (DO). A simulation model was developed to describe and evaluate the effects of several process conditions in the SBAR on N-removal performance. The model descr...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Beun JJ,Heijnen JJ,van Loosdrecht MC

    更新日期:2001-10-05 00:00:00

  • Green fluorescent protein in Saccharomyces cerevisiae: real-time studies of the GAL1 promoter.

    abstract::Green fluorescent protein (GFP) was used to study the regulation of the galactose-inducible GAL1 promoter in yeast Saccharomyces cerevisiae strains. GFP was cloned into the pGAL110 vector and transformed into the yeast strains. Time course studies comparing culture fluorescence intensity and GFP concentration were con...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Li J,Wang S,VanDusen WJ,Schultz LD,George HA,Herber WK,Chae HJ,Bentley WE,Rao G

    更新日期:2000-10-20 00:00:00

  • Metabolic modeling for predicting VFA production from protein-rich substrates by mixed-culture fermentation.

    abstract::Proteinaceous organic wastes are suitable substrates to produce high added-value products in anaerobic mixed-culture fermentations. In these processes, the stoichiometry of the biotransformation depends highly on operational conditions such as pH or feeding characteristics and there are still no tools that allow the p...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Regueira A,Lema JM,Carballa M,Mauricio-Iglesias M

    更新日期:2020-01-01 00:00:00

  • Synthesis of polyketides from low cost substrates by the thermotolerant yeast Kluyveromyces marxianus.

    abstract::Kluyveromyces marxianus is a promising nonconventional yeast for biobased chemical production due to its rapid growth rate, high TCA cycle flux, and tolerance to low pH and high temperature. Unlike Saccharomyces cerevisiae, K. marxianus grows on low-cost substrates to cell densities that equal or surpass densities in ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: McTaggart TL,Bever D,Bassett S,Da Silva NA

    更新日期:2019-07-01 00:00:00

  • Invertase biosynthesis by Saccharomyces carlsbergensis in batch and continuous cultures.

    abstract::The biosynthesis of invertase by Saccharomyces carlsbergensis LAM 1068 was studied in relation to its glucose effect at both unsteady and steady states of growth. Experimental correlations between the dilution rate and invertase specific activity (E/X) in chemostat cultures led to an optimum for the enzyme synthesis a...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Toda K

    更新日期:1976-08-01 00:00:00

  • Process parameter shifting: Part I. Effect of DOT, pH, and temperature on the performance of Epo-Fc expressing CHO cells cultivated in controlled batch bioreactors.

    abstract::The impact of process environment changes on process performance is one of the most crucial process safety issues when cultivating mammalian cells in a bioreactor. In contrast, directed shifting of process parameters can also be used as an optimization tool providing higher cell and product yields. Compared to other s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Trummer E,Fauland K,Seidinger S,Schriebl K,Lattenmayer C,Kunert R,Vorauer-Uhl K,Weik R,Borth N,Katinger H,Müller D

    更新日期:2006-08-20 00:00:00

  • Saccharification, fermentation, and protein recovery from low-temperature AFEX-treated coastal bermudagrass.

    abstract::Coastal bermudagrass was pretreated by a low-temperature ammonia fiber explosion (AFEX) process, which soaked the grass in liquid ammonia and then explosively released the pressure. Saccharifying enzymes were systematically applied to the AFEX-treated grass corresponding to low, medium, and high loadings of cellulase/...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Holtzapple MT,Ripley EP,Nikolaou M

    更新日期:1994-11-05 00:00:00

  • Kinetic studies on the aggregation of Aspergillus niger conidia.

    abstract::Morphology has a crucial effect on productivity and the supply of substrate for cultures of filamentous fungi. However, cultivation parameters leading to the desired morphology are often chosen empirically as the mechanisms governing the processes involved are usually unknown. For coagulating microorganisms like Asper...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Grimm LH,Kelly S,Hengstler J,Göbel A,Krull R,Hempel DC

    更新日期:2004-07-20 00:00:00

  • Enzymatic acylation of hydroxypropyl cellulose in organic media and determination of ester formation by diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy.

    abstract::Esters were prepared by acylation of hydroxypropyl cellulose with fatty acid catalyzed by immobilized lipase from Candida antarctica in tert-butanol. The nature of the substrates used, the initial water activity of the system, and the molecular weight of the hydroxypropyl cellulose were investigated. Moreover, Fourier...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Sereti V,Stamatis H,Pappas C,Polissiou M,Kolisis F

    更新日期:2001-02-20 00:00:00

  • Live cell imaging compatible immobilization of Chlamydomonas reinhardtii in microfluidic platform for biodiesel research.

    abstract::This paper describes a novel surface immobilization method for live-cell imaging of Chlamydomonas reinhardtii for continuous monitoring of lipid droplet accumulation. Microfluidics allows high-throughput manipulation and analysis of single cells in precisely controlled microenvironment. Fluorescence imaging based quan...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Park JW,Na SC,Nguyen TQ,Paik SM,Kang M,Hong D,Choi IS,Lee JH,Jeon NL

    更新日期:2015-03-01 00:00:00

  • Two-stage carbon distribution and cofactor generation for improving l-threonine production of Escherichia coli.

    abstract::L-Threonine, a kind of essential amino acid, has numerous applications in food, pharmaceutical, and aquaculture industries. Fermentative l-threonine production from glucose has been achieved in Escherichia coli. However, there are still several limiting factors hindering further improvement of l-threonine productivity...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Liu J,Li H,Xiong H,Xie X,Chen N,Zhao G,Caiyin Q,Zhu H,Qiao J

    更新日期:2019-01-01 00:00:00

  • Two-stage fermentation with bacteriophage lambda as an expression vector in Escherachia coli.

    abstract::The potential of bacteriophage lambda as an expression vector for a large scale production of cloned-gene proteins was evaluated in batch and continuous bioreactors using a temperature-sensitive mutant in the cl gene, which allows a simple manipulation of temperature as a means to control the phage in the lysogenic or...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Park TH,Seo JH,Lim HC

    更新日期:1991-02-20 00:00:00

  • Affinity purification of plasmid DNA directly from crude bacterial cell lysates.

    abstract::We have shown previously that a sequence-specific DNA-binding protein based on the Lac repressor protein can isolate pre-purified DNA efficiently from simple buffer solution but our attempts to purify plasmids directly from crude starting materials were disappointing with impractically low DNA yields. We have optimize...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Darby RA,Forde GM,Slater NK,Hine AV

    更新日期:2007-12-01 00:00:00

  • Use of the abiotic proton balance for determining linear relations among net conversion rates of primary metabolites in fermentation processes.

    abstract::Linear relations among net conversion rates of primary metabolites in fermentation processes can be determined by using the macroscopic or pathway balancing methods. It is shown that the process observability can be improved by incorporating both the abiotic proton balance and the pH control reagent addition rate meas...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Siano SA

    更新日期:1996-12-20 00:00:00

  • Insulin receptor antibody-iduronate 2-sulfatase fusion protein: pharmacokinetics, anti-drug antibody, and safety pharmacology in Rhesus monkeys.

    abstract::Mucopolysaccharidosis (MPS) Type II is caused by mutations in the gene encoding the lysosomal enzyme, iduronate 2-sulfatase (IDS). The majority of MPSII cases affect the brain. However, enzyme replacement therapy with recombinant IDS does not treat the brain, because IDS is a large molecule drug that does not cross th...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Boado RJ,Ka-Wai Hui E,Zhiqiang Lu J,Pardridge WM

    更新日期:2014-11-01 00:00:00

  • Modeling and analysis of radial flow mammalian cell culture.

    abstract::A model for radial flow mammalian cell culture has been developed. Two situations, one with permeable hollow fibers and the other with a porous matrix in the annulus, were considered. The hollow fibers were modeled as continuously distributed sinks. Numerical solutions are presented for the complete model as well as l...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Tharakan JP,Chau PC

    更新日期:1987-04-01 00:00:00

  • Dynamic tradeoffs in the raft-mediated entry of human immunodeficiency virus type 1 into cells.

    abstract::To initiate an infection human immunodeficiency virus type 1 (HIV-1) particles must first bind to receptors on the surface of their host cells, a process that eventually leads to fusion of viral and cellular membranes and release of the viral genome into the cytoplasm. Understanding the molecular mechanisms of these p...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Lim KI,Yin J

    更新日期:2006-02-05 00:00:00

  • Microbial utilization of levoglucosan in wood pyrolysate as a carbon and energy source.

    abstract::The Waterloo Fast Pyrolysis Process (WFPP) can produce an organic liquid high in levoglucosan (1, 6-anhydro-beta-D-glucopyranose) content from suitably pretreated lignocellulosics. A variety of fungi and yeasts were screened for their ability to utilize and ferment this organic liquid. To enhance its fermentability, t...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Prosen EM,Radlein D,Piskorz J,Scott DS,Legge RL

    更新日期:1993-08-05 00:00:00