Secondary follicle growth and oocyte maturation by culture in alginate hydrogel following cryopreservation of the ovary or individual follicles.

abstract：:Exchange of enzyme-bound H(2)O with T(2)O in aqueous solution followed by freeze drying provided tritiated water bound to chymotrypsin, subtilisin Carlsberg, and horseradish peroxidase. The desorption of T(2)O from these enzymes suspended in various organic solvents showed that all three enzymes lost enzyme-bound wate...

journal_title：Biotechnology and bioengineering

authors： Gorman LA,Dordick JS

更新日期：1992-02-20 00:00:00

abstract：:Two strains of Pseudomonas able to grow on phenol or p-nitrophenol (PNP) were isolated from sewage. Pseudomonas sp. PN101 mineralized and formed nitrite from PNP but did not mineralize phenol, and Pseudomonas sp. PH111 mineralized phenol but not PNP. Phenol increased the lag period before Pseudomonas sp. PN101 grew on...

journal_title：Biotechnology and bioengineering

authors： Murakami Y,Alexander M

更新日期：1989-02-20 00:00:00

abstract：:Dilute aqueous solutions of polymers released by marine phytoplankton (microalgae) were shown to effectively reduce drag in capillary pipe flow. Tests were performed in a capillary turbulent flow viscometer which extruded small samples under high pressures. In all, 22 species were screened, and the products of one chl...

journal_title：Biotechnology and bioengineering

authors： Ramus J,Kenney BE,Shaughnessy EJ

更新日期：1989-01-25 00:00:00

abstract：:The influence of biofilm areal cell density, species composition, and the presence of abiotic particles on the disinfection and removal of bacterial biofilms by monochloramine was investigated. Mono- and binary population biofilms of Pseudomonas aeruginosa and Klebsiella pneumoniae were grown on stainless-steel slides...

journal_title：Biotechnology and bioengineering

authors： Srinivasan R,Stewart PS,Griebe T,Chen CI,Xu X

更新日期：1995-06-20 00:00:00

abstract：:Perfusion bioreactors have been used in different tissue engineering applications because of their consistent distribution of nutrients and flow-induced shear stress within the tissue-engineering scaffold. A widely used configuration uses a scaffold with a circular cross-section enclosed within a cylindrical chamber a...

journal_title：Biotechnology and bioengineering

authors： Hidalgo-Bastida LA,Thirunavukkarasu S,Griffiths S,Cartmell SH,Naire S

更新日期：2012-04-01 00:00:00

abstract：:The effective diffusivity of oxygen, D(e), in Ca-alginate and PVA-SbQ gels was measured using a two-chamber vessel with a membrane between the two chambers. The effect of cell density, C(c), on D(e) in Ca-alginate gels was studied. The effective diffusivity of oxygen decreased with increasing cell density, to C(c) = 1...

journal_title：Biotechnology and bioengineering

authors： Sun Y,Furusaki S,Yamauchi A,Ichimura K

更新日期：1989-06-05 00:00:00

abstract：:Cytochrome c-oxidase, used as a model membrane-bound enzyme, was embedded in phospholipid bilayer membranes, attached to nanometer-sized Fe(3)O(4) colloids (so-called magnetoliposomes). In comparison with the lipid-depleted free enzyme, both the activity and the enzymatic stability of the complexes, stored at 4 degree...

journal_title：Biotechnology and bioengineering

authors： De Cuyper M,De Meulenaer B,Van der Meeren P,Vanderdeelen J

更新日期：1996-03-20 00:00:00

abstract：:The production of acetate by aerobically growing E. coli is examined. The problem is formulated in terms of a flow network that has as its objective maximal ATP synthesis. It is found that when loads are imposed and flux constraints exist either at the level of NADH turnover rate or the activity of a key Krebs cycle e...

journal_title：Biotechnology and bioengineering

authors： Majewski RA,Domach MM

更新日期：1990-03-25 00:00:00

abstract：:Pretreatment methods were compared with steam explosion, and differing views on the relative importance of mechanical and chemical effects were outlined. Hydrolysis was desirable; pyrolysis was undesirable. The effects of initial moisture content on steam consumption, mechanism and rate of heat transfer, pentosan solu...

journal_title：Biotechnology and bioengineering

authors： Brownell HH,Saddler JN

更新日期：1987-02-01 00:00:00

abstract：:The secretion of heterologous IgG proteins in the baculovirus-insect cell expression system is accompanied by substantial insoluble immunoglobulin in the infected cells. The accumulation of these insoluble forms suggests a limitation in the processing and secretory pathway of the infected cells. As a result, cytosolic...

journal_title：Biotechnology and bioengineering

authors： Ailor E,Betenbaugh MJ

更新日期：1998-04-20 00:00:00

abstract：:Identification of conditions for guided and specific differentiation of human stem cell and progenitor cells is important for continued development and engineering of in vitro cell culture systems for use in regenerative medicine, drug discovery, and human toxicology. Three-dimensional (3D) and organotypic cell cultur...

journal_title：Biotechnology and bioengineering

authors： Nierode GJ,Gopal S,Kwon P,Clark DS,Schaffer DV,Dordick JS

更新日期：2019-01-01 00:00:00

abstract：:The fim inversion system of Escherichia coli (E. coli) can behave as a unidirectional switch in an efficient manner. We have developed a new expression system for E. coli, comprising the arabinose-inducible fimE gene and the fim invertible DNA segment containing a constitutively active promoter. In this system, the ta...

journal_title：Biotechnology and bioengineering

authors： Ham TS,Lee SK,Keasling JD,Arkin AP

更新日期：2006-05-05 00:00:00

abstract：:Numerous biotechnological production processes are based on the submerse cultivation of filamentous fungi. Process design, however, is often hampered by the complex growth pattern of these organisms. In the morphologic development of coagulating filamentous fungi, like Aspergillus niger, conidial aggregation is the fi...

journal_title：Biotechnology and bioengineering

authors： Lin PJ,Grimm LH,Wulkow M,Hempel DC,Krull R

更新日期：2008-02-01 00:00:00

abstract：:Intracellular events that take place during influenza virus replication in animal cells are well understood qualitatively. However, to better understand the complex interaction of the virus with its host cell and to quantitatively analyze the use of cellular resources for virion formation or the overall dynamic for th...

journal_title：Biotechnology and bioengineering

authors： Sidorenko Y,Reichl U

更新日期：2004-10-05 00:00:00

abstract：:Transfection with polyethylenimine (PEI) was evaluated as a method for the generation of recombinant Chinese hamster ovary (CHO DG44) cell lines by direct comparison with calcium phosphate-DNA coprecipitation (CaPO4) using both green fluorescent protein (GFP) and a monoclonal antibody as reporter proteins. Following t...

journal_title：Biotechnology and bioengineering

authors： Chenuet S,Martinet D,Besuchet-Schmutz N,Wicht M,Jaccard N,Bon AC,Derouazi M,Hacker DL,Beckmann JS,Wurm FM

更新日期：2008-12-01 00:00:00

abstract：:The specific molecular interactions of alpha-helical peptide, human glucagon (i.e., intermolecular self-association and specific receptor-binding affinity) provided a rationale for using the glucagon as the fusion expression partner to achieve high productivity of foreign proteins both in vivo (in bacterial fusion-exp...

journal_title：Biotechnology and bioengineering

authors： Kim DY,Lee J,Saraswat V,Park YH

更新日期：2000-08-20 00:00:00

abstract：:The patterning of cardiac myocytes on a micron scale ( approximately 5 microm) was achieved by microcontact printing of fibronectin onto a hydrophobically pretreated glass substrate. The patterned cardiac myocytes conjugated with each other by forming a gap junction, as judged from the synchronized Ca(2+) transition o...

journal_title：Biotechnology and bioengineering

authors： Kaji H,Takoh K,Nishizawa M,Matsue T

更新日期：2003-03-20 00:00:00

abstract：:The paper reviews the state-of-the-art in computational modeling of thrombus formation and growth and related phenomena including platelet margination, activation, adhesion, and embolization. Presently, there is a high degree of empiricism in the modeling of thrombus formation. Based on the experimentally observed phy...

journal_title：Biotechnology and bioengineering

authors： Hosseinzadegan H,Tafti DK

更新日期：2017-10-01 00:00:00

abstract：:Fibrous proteins, such as silk, elastin and collagen are finding broad impact in biomaterial systems for a range of biomedical and industrial applications. Some of the key advantages of biosynthetic fibrous proteins compared to synthetic polymers include the tailorability of sequence, protein size, degradation pattern...

journal_title：Biotechnology and bioengineering

authors： Yigit S,Dinjaski N,Kaplan DL

更新日期：2016-05-01 00:00:00

abstract：:We demonstrate a novel application of (13)C pulsed field gradient (PFG) NMR to monitor mass transfer, due to both flow and diffusion, in a 3D complex porous support structure modified by biofilm growth. This enables timescales an order of magnitude larger than previously possible to be accessed with respect to displac...

journal_title：Biotechnology and bioengineering

authors： Graf von der Schulenburg DA,Akpa BS,Gladden LF,Johns ML

更新日期：2008-10-15 00:00:00

abstract：:Increases in our understanding of gene function have greatly expanded the repertoire of possible genetic interventions at our disposal with the consequence that many genetic engineering applications require multiple manipulations in which target genes can be both overexpressed and silenced in a simple and co-ordinated...

journal_title：Biotechnology and bioengineering

authors： Greber D,Fussenegger M

更新日期：2007-04-01 00:00:00

abstract：:Using (13)C-labeled glucose fed to a penicillin-overproducing strain of Penicillium chrysogenum, the intracellular fluxes were quantified, and the presence of two new pathways, not previously described in this organism, is suggested. Thus, glycine was synthesized not only by serine hydroxymethyltransferase, but also b...

journal_title：Biotechnology and bioengineering

authors： Christensen B,Nielsen J

更新日期：2000-06-20 00:00:00

abstract：:Beta defensins are antimicrobial peptides (AMPs) with a broad spectrum antimicrobial behavior against pathogens while having minimal tendency to incur pathogen resistance. Human β-defensin 28 (hBD28) is a strongly cationic AMP and hence hypothesized to be highly effective in permeabilizing negatively-charged pathogen ...

journal_title：Biotechnology and bioengineering

authors： Tay DK,Rajagopalan G,Li X,Chen Y,Lua LH,Leong SS

更新日期：2011-03-01 00:00:00

abstract：:Batch fermentations of glucose to ethanol by Z. Mobilis.(ATCC 10988) were examined in several semidefined nutrient media. The measurement of acid produced by the microorganism was used to study its transient fermentation characteristics. Limitation of nitrogen source in the semidefined medium of Rogers and coworkers(2...

journal_title：Biotechnology and bioengineering

authors： Veeramallu U,Agrawal P

更新日期：1988-05-20 00:00:00

abstract：:For in vitro liver replacement devices, such as packed bed bioreactors, to maintain the essential functions of the liver, they must at least successfully support hepatocytes, the parenchymal cell of the liver. In vivo, the liver is a major consumer of oxygen. Hence it is unsurprising that the limited transport distanc...

journal_title：Biotechnology and bioengineering

authors： Niu M,Clemens MG,Coger RN

更新日期：2008-04-15 00:00:00

abstract：:Membrane adsorbers may be a viable alternative to the packed-bed chromatography for clearance of virus, host cell proteins, DNA, and other trace impurities. However, incorporation of membrane adsorbers into manufacturing processes has been slow due to the significant cost associated with obtaining regulatory approval ...

journal_title：Biotechnology and bioengineering

authors： Weaver J,Husson SM,Murphy L,Wickramasinghe SR

更新日期：2013-02-01 00:00:00

abstract：:Tumor-targeted Salmonella VNP20009 preferentially replicate within tumor tissue and partially suppress tumor growth in murine tumor models. These Salmonella have the ability to locally induce apoptosis when they are in direct contact with cancer cells but they lack significant bystander killing, which may correlate wi...

journal_title：Biotechnology and bioengineering

authors： Quintero D,Carrafa J,Vincent L,Bermudes D

更新日期：2016-12-01 00:00:00

abstract：:A framework using material balances in metabolic pathways to study cellular metabolism is examined and the results are discussed. Rate measurements on extracellular compounds alone were found to be not always sufficient to validate proposed unique intracellular mechanisms. The conditions to delineate among candidate m...

journal_title：Biotechnology and bioengineering

authors： Niranjan SC,San KY

更新日期：1989-08-05 00:00:00

abstract：:Cancer is the leading cause of death in industrialized countries. Cancer therapy often involves monoclonal antibodies or small-molecule drugs, but carbohydrate-binding lectins such as mistletoe (Viscum album) viscumin offer a potential alternative treatment strategy. Viscumin is toxic in mammalian cells, ruling them o...

journal_title：Biotechnology and bioengineering

authors： Gengenbach BB,Keil LL,Opdensteinen P,Müschen CR,Melmer G,Lentzen H,Bührmann J,Buyel JF

更新日期：2019-09-01 00:00:00

abstract：:The S-System formalism provides a popular, versatile and mathematically tractable representation of metabolic pathways. At steady-state, after a logarithmic transformation, the S-System representation reduces into a system of linear equations. Thus, the maximization of a particular metabolite concentration or a flux s...

journal_title：Biotechnology and bioengineering

authors： Petkov SB,Maranas CD

更新日期：1997-10-20 00:00:00