A new bioproduction route for a novel antimicrobial peptide.

abstract：:The utilization and conversion of D-xylose, D-xylulose, L-arabinose, and xylitol by yeast strains have been investigated with the following results: (1) The majority of yeasts tested utilize D-xylose and produce polyols, ethanol, and organic acids. The type and amount of products formed varies with the yeast strains u...

journal_title：Biotechnology and bioengineering

authors： Gong CS,Claypool TA,McCracken LD,Maun CM,Ueng PP,Tsao GT

更新日期：1983-01-01 00:00:00

abstract：:We demonstrate a novel application of (13)C pulsed field gradient (PFG) NMR to monitor mass transfer, due to both flow and diffusion, in a 3D complex porous support structure modified by biofilm growth. This enables timescales an order of magnitude larger than previously possible to be accessed with respect to displac...

journal_title：Biotechnology and bioengineering

authors： Graf von der Schulenburg DA,Akpa BS,Gladden LF,Johns ML

更新日期：2008-10-15 00:00:00

abstract：:Protein partitioning in aqueous two-phase systems based on phase-forming polymers is strongly affected by the net charge of the protein, but a thermodynamic description of the charge effects has been hindered by conflicting results. Many of the difficulties could be because of problems in isolating electrochemical eff...

journal_title：Biotechnology and bioengineering

authors： Fan W,Bakir U,Glatz CE

更新日期：1998-08-20 00:00:00

abstract：:Control of cell shape and behavior through the micropattern technique by spatial immobilization of adhesive proteins on a surface has provided novel insights in several aspects of cell biology, such as tissue morphogenesis, cell growth and cell differentiation, and apoptosis. In this work, we present the use of poly(e...

journal_title：Biotechnology and bioengineering

authors： Racine J,Cheradame H,Chu YS,Thiery JP,Rodriguez I

更新日期：2011-04-01 00:00:00

abstract：:Two strains of Pseudomonas able to grow on phenol or p-nitrophenol (PNP) were isolated from sewage. Pseudomonas sp. PN101 mineralized and formed nitrite from PNP but did not mineralize phenol, and Pseudomonas sp. PH111 mineralized phenol but not PNP. Phenol increased the lag period before Pseudomonas sp. PN101 grew on...

journal_title：Biotechnology and bioengineering

authors： Murakami Y,Alexander M

更新日期：1989-02-20 00:00:00

abstract：:Increasing demand for petroleum has stimulated industry to develop sustainable production of chemicals and biofuels using microbial cell factories. Fatty acids of chain lengths from C6 to C16 are propitious intermediates for the catalytic synthesis of industrial chemicals and diesel-like biofuels. The abundance of gen...

journal_title：Biotechnology and bioengineering

authors： Tee TW,Chowdhury A,Maranas CD,Shanks JV

更新日期：2014-05-01 00:00:00

abstract：:Chlorella sp. strain VJ79 was isolated from a total heterotrophic count of a wastewater collector. It grows autotrophically, heterotrophically, and mixotrophically on a variety of organic substrates. Glucose and serine promote a mixotrophic growth from which the yield is higher than the sum of autotrophic and heterotr...

journal_title：Biotechnology and bioengineering

authors： Lalucat J,Imperial J,Parés R

更新日期：1984-07-01 00:00:00

abstract：:Nutrient utilization and byproduct accumulation were monitored in Spodoptera frugiperda Sf-9 and Trichoplusia ni BTI-Tn-5B1-4 (High Five) cell lines during growth and following viral infection in suspension cultures in order to develop a better understanding of cell metabolism and to acquire information relevant to la...

journal_title：Biotechnology and bioengineering

authors： Rhiel M,Mitchell-Logean CM,Murhammer DW

更新日期：1997-09-20 00:00:00

abstract：:A complete, integrated process for the production of an innovative formulation of penicillin G acylase from Providencia rettgeri(rPAC(P.rett))of industrial applicability is reported. In order to improve the yield of rPAC, the clone LN5.5, carrying four copies of pac gene integrated into the genome of Pichia pastoris, ...

journal_title：Biotechnology and bioengineering

authors： Senerovic L,Stankovic N,Spizzo P,Basso A,Gardossi L,Vasiljevic B,Ljubijankic G,Tisminetzky S,Degrassi G

更新日期：2006-02-05 00:00:00

abstract：:The thermo-responsive polymer poly(N-isopropylacrylamide) has received widespread attention for its in vitro application in the non-invasive, non-destructive release of adherent cells on two dimensional surfaces. In this study, 3D non-woven scaffolds fabricated from poly(propylene) (PP), poly(ethylene terephthalate) (...

journal_title：Biotechnology and bioengineering

authors： Rossouw CL,Chetty A,Moolman FS,Birkholtz LM,Hoppe H,Mancama DT

更新日期：2012-08-01 00:00:00

abstract：:Flow visualization using fluorescent microparticles and cell viability investigations are carried out to examine the mechanisms by which cells are seeded into scaffolds driven by surface acoustic waves. The former consists of observing both the external flow prior to the entry of the suspension into the scaffold and t...

journal_title：Biotechnology and bioengineering

authors： Bok M,Li H,Yeo LY,Friend JR

更新日期：2009-06-01 00:00:00

abstract：:Exchange of enzyme-bound H(2)O with T(2)O in aqueous solution followed by freeze drying provided tritiated water bound to chymotrypsin, subtilisin Carlsberg, and horseradish peroxidase. The desorption of T(2)O from these enzymes suspended in various organic solvents showed that all three enzymes lost enzyme-bound wate...

journal_title：Biotechnology and bioengineering

authors： Gorman LA,Dordick JS

更新日期：1992-02-20 00:00:00

abstract：:Measurement of the surface roughness and thickness of biological films is laborious and usually destructive, thus hampering research in this area. We developed a laser triangulation sensor (LTS) set-up for the fast and nondestructive measurement of these biofilm parameters during growth. Using LTS measurements, the mo...

journal_title：Biotechnology and bioengineering

authors： Okkerse WJ,Ottengraf SP,Osinga-Kuipers B

更新日期：2000-12-20 00:00:00

abstract：:Non-invasive methods for online monitoring of biotechnological processes without compromising the integrity of the reactor system are very important to generate continuous data. Even though calorimetry has been used in conventional biochemical analysis for decades, it has not yet been specifically applied for online d...

journal_title：Biotechnology and bioengineering

authors： Regestein L,Maskow T,Tack A,Knabben I,Wunderlich M,Lerchner J,Büchs J

更新日期：2013-05-01 00:00:00

abstract：:The activities of lipase from Candida cylindracea and Rhizopus delemar have been investigated in water/AOT/iso-octane reverse micellar media through the use of two esterification reactions: fatty acid-alcohol esterification and glyceride synthesis. Such media promotes the occurrence of these two lipase-catalyzed react...

journal_title：Biotechnology and bioengineering

authors： Hayes DG,Gulari E

更新日期：1990-04-05 00:00:00

abstract：:Embryonic stem cells (ESCs) are an important source of cardiomyocytes for regenerating injured myocardium. The successful use of ESC-derived cardiomyocytes in cardiac tissue engineering requires an understanding of the important scaffold properties and culture conditions to promote cell attachment, differentiation, or...

journal_title：Biotechnology and bioengineering

authors： Parrag IC,Zandstra PW,Woodhouse KA

更新日期：2012-03-01 00:00:00

abstract：:Dielectric spectroscopy was applied to two industrial high cell density culture processes and used to determine on-line the concentration of CHO cells immobilized on macroporous microcarriers in a stirred bioreactor and in a packed-bed of disk carriers. The cell concentration predicted from the spectroscopic data was ...

journal_title：Biotechnology and bioengineering

authors： Ducommun P,Kadouri A,von Stockar U,Marison IW

更新日期：2002-02-05 00:00:00

abstract：:Novel amphoteric particles are prepared by an interfacial polyaddition reaction of ethylene glycol diglycidyl ether (EGDGE) and L-lysine in a water-in-oil (W/O) miniemulsion system. The zeta-potential of the copolymer particle as a function of pH indicates a sigmoid curve with an isoelectric point at pH 8.7 due to the...

journal_title：Biotechnology and bioengineering

authors： Taira S,Du YZ,Kodaka M

更新日期：2006-02-05 00:00:00

abstract：:The study reports on the development of a bioreactor for the production of alpha-keto acids from D,L- or D-amino acids using Rhodotorula gracilis D-amino acid oxidase. D-amino acid oxidase was co-immobilized with catalase on Affi-Gel 10 matrix, and the reactor was operated as a continuous-stirred tank reactor (CSTR) o...

journal_title：Biotechnology and bioengineering

authors： Butò S,Pollegioni L,D'Angiuro L,Pilone MS

更新日期：1994-12-01 00:00:00

abstract：:The regeneration of functional, clinically viable, tissues from acellular ex vivo tissues has been problematic largely due to poor nutrient transport conditions that limit cell migration and integration. Compounding these issues are subcellular pore sizes that necessarily requires extracellular matrix (ECM) remodeling...

journal_title：Biotechnology and bioengineering

authors： Tosun Z,McFetridge PS

更新日期：2013-07-01 00:00:00

abstract：:A method was developed for obtaining high signal-to-noise 13C NMR spectra of intracellular compounds in metabolically active cultured cells. The method allows TCA cycle labeling kinetics to be determined in real time without significant oxygen transport limitations. Cells were immobilized on the surface of nonporous m...

journal_title：Biotechnology and bioengineering

authors： Mancuso A,Beardsley NJ,Wehrli S,Pickup S,Matschinsky FM,Glickson JD

更新日期：2004-09-30 00:00:00

abstract：:The specific molecular interactions of alpha-helical peptide, human glucagon (i.e., intermolecular self-association and specific receptor-binding affinity) provided a rationale for using the glucagon as the fusion expression partner to achieve high productivity of foreign proteins both in vivo (in bacterial fusion-exp...

journal_title：Biotechnology and bioengineering

authors： Kim DY,Lee J,Saraswat V,Park YH

更新日期：2000-08-20 00:00:00

abstract：:The performance of an industrial pharmaceutical process (production of an active pharmaceutical ingredient by fermentation, API) was modeled by multiblock partial least squares (MBPLS). The most important process stages are inoculum production and API production fermentation. Thirty batches (runs) were produced accord...

journal_title：Biotechnology and bioengineering

authors： Lopes JA,Menezes JC,Westerhuis JA,Smilde AK

更新日期：2002-11-20 00:00:00

abstract：:A computer-controlled headspace gas chromatograph was used to monitor the progress of ethanol production from both aerobic batch and anaerobic continuous fermentations. Using an automatic, electropneumatic sampling system, aliquots of fermentation headspace gas were injected directly onto the column for quantitative e...

journal_title：Biotechnology and bioengineering

authors： Comberbach DM,Bu'lock JD

更新日期：1983-11-01 00:00:00

abstract：:A biosensor detecting estrogens, progestogens, and androgens in complex samples and in a single step is described. Three Arxula adeninivorans yeast strains were created, each strain producing a different recombinant human hormone receptor and a different fluorescent reporter protein. These strains were then mixed to c...

journal_title：Biotechnology and bioengineering

authors： Chamas A,Pham HTM,Jähne M,Hettwer K,Uhlig S,Simon K,Einspanier A,Baronian K,Kunze G

更新日期：2017-07-01 00:00:00

abstract：:There has been a recent drive in commercial large-scale production of biotechnology products to convert current batch mode processing to continuous processing manufacturing. There have been reports of model systems capable of adapting and linking upstream and downstream technologies into a continuous manufacturing pip...

journal_title：Biotechnology and bioengineering

authors： Johnson SA,Brown MR,Lute SC,Brorson KA

更新日期：2017-01-01 00:00:00

abstract：:An alkali stable polyamidase was isolated from a new strain of Nocardia farcinica. The enzyme consists of four subunits with a total molecular weight of 190 kDa. The polyamidase cleaved amide and ester bonds of water insoluble model substrates like adipic acid bishexylamide and bis(benzoyloxyethyl)terephthalate and hy...

journal_title：Biotechnology and bioengineering

authors： Heumann S,Eberl A,Fischer-Colbrie G,Pobeheim H,Kaufmann F,Ribitsch D,Cavaco-Paulo A,Guebitz GM

更新日期：2009-03-01 00:00:00

abstract：:We originally investigated the suitability of chitosan scaffolds loaded with bone morphogenetic protein 6 (BMP-6) in both stationary and dynamic conditions for cartilage tissue engineering. In the first part of the present study, ATDC5 murine chondrogenic cells were seeded in chitosan and BMP-6 loaded chitosan scaffol...

journal_title：Biotechnology and bioengineering

authors： Tiğli RS,Gümüşderelioğlu M

更新日期：2009-10-15 00:00:00

abstract：:Pretreatment of rice straw by using renewable cholinium amino acids ionic liquids ([Ch][AA] ILs)-water mixtures and the subsequent enzymatic hydrolysis of the residues were conducted in the present work. Of the eight mixtures composed of ILs and water, most were found to be effective for rice straw pretreatment. After...

journal_title：Biotechnology and bioengineering

authors： Hou XD,Li N,Zong MH

更新日期：2013-07-01 00:00:00

abstract：:This reports on the determination of the overall oxygen transfer coefficient in a mechanically agitated vessel using a randomly pulsed dynamic method. This method consists in exciting the system by randomly switching the inlet gas stream with air or nitrogen with an identical volumetric flow rate. A pseudo-random bina...

journal_title：Biotechnology and bioengineering

authors： Gauthier L,Thibault J,LeDuy A

更新日期：1991-04-15 00:00:00