Esterification reactions of lipase in reverse micelles.

abstract：:While many pretreatments attempt to improve the enzymatic digestibility of biomass by removing lignin, this study shows that improving the surface area accessible to cellulase is a more important factor for achieving a high sugar yield. Here we compared the pretreatment of switchgrass by two methods, cellulose solvent...

journal_title：Biotechnology and bioengineering

authors： Rollin JA,Zhu Z,Sathitsuksanoh N,Zhang YH

更新日期：2011-01-01 00:00:00

abstract：:The Chinese hamster ovary (CHO) cell line producing interferon-gamma (IFN-gamma) exhibits a 2-fold increase in specific productivity when grown at 32 degrees C compared to 37 degrees C. Low temperature also causes growth arrest, meaning that the cell density is significantly lower at 32 degrees C, nutrients are consum...

journal_title：Biotechnology and bioengineering

authors： Fox SR,Patel UA,Yap MG,Wang DI

更新日期：2004-01-20 00:00:00

abstract：:Many sites of environmental concern contain groundwater contaminated with nonaqueous phase liquids (NAPL). In such sites interfacial processes may affect both the equilibrium and kinetic behavior of the system. In particular, insoluble hydrocarbon partitioning and microbial biodegradation of insoluble hydrocarbon are ...

journal_title：Biotechnology and bioengineering

authors： Sekelsky AM,Shreve GS

更新日期：1999-05-20 00:00:00

abstract：:Phage Φ80 can infect Escherichia coli in a stealthy manner and persist by forming lysogens. Such Φ80 lysogens are fairly common and often go undetected unless the host is grown at temperatures below 37°C. Since low growth temperatures are required for growing temperature-sensitive mutants and often preferred for large...

journal_title：Biotechnology and bioengineering

authors： Srinivas S,Cronan JE

更新日期：2019-07-01 00:00:00

abstract：:Utilising whole cells of recombinant Escherichia coli K27 (pCYP102, pGEc47) containing active cytochrome P450BM-3 monooxygenase [E.C. 1. 14.14.1], multiple oxidations of saturated and unsaturated fatty acids were performed by the enzyme under conditions of excess oxygen. The amount of oxygen dissolved in the culture m...

journal_title：Biotechnology and bioengineering

authors： Schneider S,Wubbolts MG,Oesterhelt G,Sanglard D,Witholt B

更新日期：1999-08-05 00:00:00

abstract：:The laminate consisted of several polymer layers, aluminium, and one cellulose-based layer containing the active enzymatic system (e.g., glucose oxidase, catalase, glucose, and CaCO3). During the industrial lamination process, the enzyme layer was exposed to three temperature spikes up to 325 degrees C without signifi...

journal_title：Biotechnology and bioengineering

authors： Andersson M,Andersson T,Adlercreutz P,Nielsen T,Hörnsten EG

更新日期：2002-07-05 00:00:00

abstract：:The main processes involved in enhanced biological phosphorus removal (EBPR) under anaerobic and subsequently aerobic conditions are widely described in the literature. Polyphosphate accumulating organisms (PAO) are the organisms responsible for this process. However, the mechanisms of PAO are not fully established ye...

journal_title：Biotechnology and bioengineering

authors： Guisasola A,Pijuan M,Baeza JA,Carrera J,Casas C,Lafuente J

更新日期：2004-03-30 00:00:00

abstract：:Catalytic activity of lipases (from Rhizopus arrhizus, Canadida rugosa, and Pseudomonas sp. was studied in organic media, mainly diisopropyl ether. The effect of water activity (a(w)) on V(max) showed that the enzyme activity in general increased with increasing amounts of water for the three enzymes. This was shown b...

journal_title：Biotechnology and bioengineering

authors： Wehtje E,Adlercreutz P

更新日期：1997-09-05 00:00:00

abstract：:A biotechnological process is described to remove hydrogen sulfide (H(2)S) from high-pressure natural gas and sour gases produced in the petrochemical industry. The process operates at halo-alkaline conditions and combines an aerobic sulfide-oxidizing reactor with an anaerobic sulfate (SO(4) (2-)) and thiosulfate (S(2...

journal_title：Biotechnology and bioengineering

authors： van den Bosch PL,van Beusekom OC,Buisman CJ,Janssen AJ

更新日期：2007-08-01 00:00:00

abstract：:The biotransformation of benzaldehyde and glucose to L-phenylacetylcarbinol (PAC) using Candida utilis was demonstrated in a solid-liquid two-phase partitioning bioreactor (TPPB) with the aim of reducing substrate, product, and by-product toxicity via sequestration. Previous work in the field had used octanol as the s...

journal_title：Biotechnology and bioengineering

authors： Khan TR,Daugulis AJ

更新日期：2010-11-01 00:00:00

abstract：:The enzymatic hydrolysis of olive oil using Chromobacterium viscosum lipase B encapsulated in reversed micelles of dioctyl sodium sulfosuccinate (AOT) in isooctane was investigated in an ultrafiltration ceramic membrane reactor of tubular type, operating in a batch mode. Water concentration was found to be a critical ...

journal_title：Biotechnology and bioengineering

authors： Prazers DM,Garcia FA,Cabral JM

更新日期：1993-04-05 00:00:00

abstract：:The mouse mammary tumor virus (MMTV) promoter is induced by the addition of a glucocorticoid hormone or analog such as dexamethasone. The hormone binds to its specific transcription factor, glucocorticoid receptor (GR), and the activated complex then binds to the glucocorticoid response elements (GREs) in the enhancer...

journal_title：Biotechnology and bioengineering

authors： James RI,Elton JP,Todd P,Kompala DS

更新日期：2000-01-20 00:00:00

abstract：:The United States produces more than 10 million tons of waste oils and fats each year. This paper aims to establish a new biomanufacturing platform that converts waste oils or fats into a series of value-added products. Our research employs the oleaginous yeast Yarrowia lipolytica as a case study for citric acid (CA) ...

journal_title：Biotechnology and bioengineering

authors： Liu N,Soong YV,Mirzaee I,Olsen A,Yu P,Wong HW,Xie D

更新日期：2021-01-20 00:00:00

abstract：:A facile methodology has been developed for covalently derivatizing the surfaces of common materials with a designed antibacterial polycation, poly(vinyl-N-pyridinium bromide), wherein the first, key step involves surface coating with a nanolayer of silica. Various commercial synthetic polymers derivatized in this man...

journal_title：Biotechnology and bioengineering

authors： Tiller JC,Lee SB,Lewis K,Klibanov AM

更新日期：2002-08-20 00:00:00

abstract：:Trehalase is the enzyme which hydrolyzes the disaccharide trehalose into two alpha-D-glucose molecules. In this article, we present the immobilization of trehalase on aminopropyl glass particles. The enzyme was extracted from Escherichia coli Mph2, a strain harboring the pTRE11 plasmid, which contains the trehalase ge...

journal_title：Biotechnology and bioengineering

authors： Bachinski N,Martins AS,Paschoalin VM,Panek AD,Paiva CL

更新日期：1997-04-05 00:00:00

abstract：:Cellulase (Cellulosin AC-8) was immobilized on poly-L-glutamic acid. This immobilized cellulase (IC) is water soluble in the neutral and alkaline solutions, where IC has the activity, while IC can be made insoluble by lowering the pH so that it can be recovered from the reaction mixture with its activity. The optimum ...

journal_title：Biotechnology and bioengineering

authors： Takeuchi T,Makino K

更新日期：1987-02-01 00:00:00

abstract：:One potential advantage of an extremely thermophilic metabolic engineering host (T opt ≥ 70°C) is facilitated recovery of volatile chemicals from the vapor phase of an active fermenting culture. This process would reduce purification costs and concomitantly alleviate toxicity to the cells by continuously removing solv...

journal_title：Biotechnology and bioengineering

authors： Zeldes BM,Straub CT,Otten JK,Adams MWW,Kelly RM

更新日期：2018-12-01 00:00:00

abstract：:Extractive work-up of whole-cell biotransformations generally suffers from the formation of stable gels and slimes upon addition of the organic solvent to the cell suspension and the cell-free solution, respectively. This problem has been overcome by enzymatic lysis of emulsifying agents present in the medium through ...

journal_title：Biotechnology and bioengineering

authors： Jörg G,Leppchen K,Daussmann T,Bertau M

更新日期：2004-08-20 00:00:00

abstract：:Cell morphology is an important macroscopic indicator of cellular physiology and is increasingly used as a mean of probing culture state in vitro. Phase contrast microscopy (PCM) is a valuable tool for observing live cells morphology over long periods of time with minimal culture artifact. Two general approaches are c...

journal_title：Biotechnology and bioengineering

authors： Juneau PM,Garnier A,Duchesne C

更新日期：2017-01-01 00:00:00

abstract：:Polymeric membranes are increasingly used as supports for the immobilization of enzymes in bioreactors. One of the more common reactor types employed in lipase-catalyzed hydrolysis of oils, contains modified cellulose as a membrane material. We found that this type of material is readily attacked by cellulase present ...

journal_title：Biotechnology and bioengineering

authors： Derksen JT,Boswinkel G,Cuperus FP

更新日期：1992-10-05 00:00:00

abstract：:The biological molecule responsible for the suppression of pyritic sulfur in fine coal simulated froth flotation treated with bacteria was identified. Protein was found to be the most effective agent in pyrite suppression of the three cell components (protein, lipid, and carbohydrate) assayed. Coal recovery and ash re...

journal_title：Biotechnology and bioengineering

authors： Stainthorpe AC

更新日期：1989-02-05 00:00:00

abstract：:A balance between proliferation and cell death is critical for achieving desirable high cell densities in mammalian cell culture. In this study, we evaluate a recently discovered anti-apoptotic gene, aven, and examine its effectiveness alone and in combination with a member of the Bcl-2 family, bcl-xL. The commerciall...

journal_title：Biotechnology and bioengineering

authors： Figueroa B Jr,Chen S,Oyler GA,Hardwick JM,Betenbaugh MJ

更新日期：2004-03-20 00:00:00

abstract：:The hypothesis that frequency and amplitude response can be used in a complicated metabolic pathway kinetics model for optimal parameter estimation, as speculated by its successful prior usage for a mechanical oscillator and a heterogeneous chemical system, is tested here. Given the complexity of the glycolysis model ...

journal_title：Biotechnology and bioengineering

authors： Zimmerman WB

更新日期：2005-10-05 00:00:00

abstract：:Melanoma is now the fifth most common type of cancer in North America. At present, there is no optimal treatment for this cancer. However, the lowering of the tyrosine level can inhibit the growth of melanoma. Unfortunately, this diet restriction cannot be humanly tolerated and causes vomiting, nausea, and severe body...

journal_title：Biotechnology and bioengineering

authors： Yu B,Chang TM

更新日期：2004-06-30 00:00:00

abstract：:This paper presents a new formulation for the analysis of growth kinetics on multiple nutrients. The baseline for the theory is the concept that a noninteractive growth process occurs among perfectly substitutable nutrients if the locus of points of the substrate concentrations producing equal growth rate is linear. A...

journal_title：Biotechnology and bioengineering

authors： Haas CN

更新日期：1994-06-20 00:00:00

abstract：:Pathway refactoring serves as an invaluable synthetic biology tool for natural product discovery, characterization, and engineering. However, the complicated and laborious molecular biology techniques largely hinder its application in natural product research, especially in a high-throughput manner. Here we report a p...

journal_title：Biotechnology and bioengineering

authors： Ren H,Hu P,Zhao H

更新日期：2017-08-01 00:00:00

abstract：:In crude extract-based cell-free protein synthesis (CFPS), DNA templates are transcribed and translated into functional proteins. Although linear expression templates (LETs) are less laborious and expensive to generate, plasmid templates are often desired over polymerase chain reaction-generated LETs due to increased ...

journal_title：Biotechnology and bioengineering

authors： Zhu B,Gan R,Cabezas MD,Kojima T,Nicol R,Jewett MC,Nakano H

更新日期：2020-08-20 00:00:00

abstract：:We have used a novel approach in conjunction with flow cytometry to quantify the biological heterogeneity of populations of the ciliate Tetrahymena pyriformis. It was found that the rate of particle uptake of exponentially growing cells is not uniform among cells and partially correlated with cell size. The physiologi...

journal_title：Biotechnology and bioengineering

authors： Hatzis C,Srienc F,Fredrickson AG

更新日期：1994-03-05 00:00:00

abstract：:A biosensor model was developed for a commercial NADH fluorescence probe to describe the single-frequency excitation and emission fluorescence behavior of an aqueous mixture of fluorophores. This model is essential in correlating the measured signals to the concentrations of fluorescent compounds in a bioreactor. In a...

journal_title：Biotechnology and bioengineering

authors： Wang NS,Simmons MB

更新日期：1991-12-20 00:00:00

abstract：:A method was developed for obtaining high signal-to-noise 13C NMR spectra of intracellular compounds in metabolically active cultured cells. The method allows TCA cycle labeling kinetics to be determined in real time without significant oxygen transport limitations. Cells were immobilized on the surface of nonporous m...

journal_title：Biotechnology and bioengineering

authors： Mancuso A,Beardsley NJ,Wehrli S,Pickup S,Matschinsky FM,Glickson JD

更新日期：2004-09-30 00:00:00