Abstract:
:The enzymatic hydrolysis of olive oil using Chromobacterium viscosum lipase B encapsulated in reversed micelles of dioctyl sodium sulfosuccinate (AOT) in isooctane was investigated in an ultrafiltration ceramic membrane reactor of tubular type, operating in a batch mode. Water concentration was found to be a critical parameter in the enzyme kinetics and hydrolysis yield of the reaction. The size of micelles, recirculation rate, and substrate concentration were found to be the major factors affecting the separation process. A correlation that enables the prediction of final conversion degrees in this bioreactor from the initial reaction conditions was established.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Prazers DM,Garcia FA,Cabral JMdoi
10.1002/bit.260410802subject
Has Abstractpub_date
1993-04-05 00:00:00pages
761-70issue
8eissn
0006-3592issn
1097-0290journal_volume
41pub_type
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journal_title:Biotechnology and bioengineering
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