Abstract:
:This paper presents a new formulation for the analysis of growth kinetics on multiple nutrients. The baseline for the theory is the concept that a noninteractive growth process occurs among perfectly substitutable nutrients if the locus of points of the substrate concentrations producing equal growth rate is linear. A deviation function is then defined with respect to this base case, and several models for this function are suggested. The underlying theory is taken by analogy with mixture thermodynamics. The proposed formulation is tested against data in the literature on growth under substitutable and complementary substrate mixtures. (c) 1994 John Wiley & Sons, Inc.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Haas CNdoi
10.1002/bit.260440203subject
Has Abstractpub_date
1994-06-20 00:00:00pages
154-64issue
2eissn
0006-3592issn
1097-0290journal_volume
44pub_type
杂志文章abstract::The selectivity of protein extraction by reversed micellar solutions can be improved by the addition of affinity cosurfactants bearing ligands which bind strongly to the target protein. The interactions between cosurfactant and protein, as well as the interfacial activity of both the free cosurfactant and the protein-...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260421011
更新日期:1993-11-20 00:00:00
abstract::Light-responsive bioconjugates are synthesized by a two-step protocol calling first for cotelomerization (chain-transfer polymerization) of N-isopropylacrylamide and N-acryloxysuccinimide. The desired bioligand (biotin) is used in modified form as chain-transfer agent in this step. As a consequence, 100% of the produc...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20479
更新日期:2005-09-05 00:00:00
abstract::A refolding strategy was described for on-column refolding of recombinant human interferon-gamma (rhIFN-gamma) inclusion bodies by expanded bed adsorption (EBA) chromatography. After the denatured rhIFN-gamma protein bound onto the cation exchanger of STREAMLINE SP, the refolding process was performed in expanded bed ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20763
更新日期:2006-03-05 00:00:00
abstract::A thermodynamic framework (UNIQUAC model with temperature dependent parameters) is applied to model the salt-induced protein crystallization equilibrium, i.e., protein solubility. The framework introduces a term for the solubility product describing protein transfer between the liquid and solid phase and a term for th...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990720)64:2<144::aid-bit
更新日期:1999-07-20 00:00:00
abstract::Maintenance of high cell viability was the main characteristic of our new strains of thermotolerant Saccharomyces. Total sugar conversion to ethanol was observed for sugarcane juice fermentation at 38-40 degrees C in less than 10 h and without continuous aeration of the culture. Invertase activity differed among the s...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260370606
更新日期:1991-03-15 00:00:00
abstract::Biofilm growth in porous media is difficult to study non-invasively due to the opaqueness and heterogeneity of the systems. Magnetic resonance is utilized to non-invasively study water dynamics within porous media. Displacement-relaxation correlation experiments were performed on fluid flow during biofilm growth in a ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24803
更新日期:2013-05-01 00:00:00
abstract::A technique has been developed for characterizing the in vivo behavior of key enzymes from intermediate measurements. The technique is based on the identification of characteristic reaction paths, and it depends on the time scale separation characteristics of the systems. It is shown that useful information can be obt...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260310813
更新日期:1988-05-20 00:00:00
abstract::A new ultrafiltration technique based on a multimembrane stack has been developed to fractionate solutes closer in size than conventionally possible. The technique is illustrated here by obtaining a pure protein product from a binary protein mixture. By employing membranes in series without any gaskets or spacers in-b...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20069
更新日期:2004-06-20 00:00:00
abstract::A model is proposed that accounts for the decreases in yield which occur in chemostat cultures of mesophilic yeasts at superoptimal growth temperatures. Two yield depressing effects were identified, one due to increased maintenance requirements by the viable fraction of the population, the other due to energy substrat...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260180603
更新日期:1976-06-01 00:00:00
abstract::Poly lactic acid (PLA) based plastics is renewable, bio-based, and biodegradable. Although present day PLA is composed of mainly L-LA, an L- and D- LA copolymer is expected to improve the quality of PLA and expand its use. To increase the number of thermotolerant microbial biocatalysts that produce D-LA, a derivative ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26472
更新日期:2018-02-01 00:00:00
abstract::Mass balances were performed on an alkaline lysis operation for the primary recovery of supercoiled plasmid DNA as part of a process for plasmid gene preparation. Escherichia coli DH5alpha/pSVbeta was cultured in defined medium by fed-batch fermentation and harvested at the end of the exponential phase. Alkaline lysis...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10166
更新日期:2002-03-30 00:00:00
abstract::The attrition bioreactor (ABR) combines wet ball milling and enzymatic hydrolysis in one process step. It was found that the ABR did not accelerate enzyme deactivation. Interfacial forces, not shear forces, caused the most deactivation. Elimination of the air-liquid interface by covering the reactor substantially incr...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260310106
更新日期:1988-01-01 00:00:00
abstract::Exchange of enzyme-bound H(2)O with T(2)O in aqueous solution followed by freeze drying provided tritiated water bound to chymotrypsin, subtilisin Carlsberg, and horseradish peroxidase. The desorption of T(2)O from these enzymes suspended in various organic solvents showed that all three enzymes lost enzyme-bound wate...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390405
更新日期:1992-02-20 00:00:00
abstract::In order to eliminate organic pollutants in waste gases, a biological filter bed technique has been developed, with a high self-regenerating capacity and a low pressure drop. The bed consists of an appropriate filling material (mainly peat compost), in order to let the microorganisms grow on the solid surface and to s...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260251222
更新日期:1983-12-01 00:00:00
abstract::Dihydrofolate reductase (DHFR) system is used to amplify the product gene to multiple copies in Chinese Hamster Ovary (CHO) cells for generating cell lines which produce the recombinant protein at high levels. The physiological changes accompanying the transformation of the non-protein secreting host cells to a high p...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25117
更新日期:2014-03-01 00:00:00
abstract::Foam separation may have potential for protein recovery. However, for foam separation to be a viable protein recovery technique it is important to demonstrate, not only that high enrichments and recoveries can be achieved for single proteins, but also that high enrichments and recoveries, together with selectivity of ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990205)62:3<291::aid-bit
更新日期:1999-02-05 00:00:00
abstract::Urea, ammonium, and free amino acid contents were quantified in biological aging of a young wine under two flor film forming yeast strains, Saccharomyces cerevisiae race capensis and S. cerevisiae race bayanus, and compared. Cell viability in the film was different for the two yeast strains. Thus, capensis maintained ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19970120)53:2<159::AID-BIT
更新日期:1997-01-20 00:00:00
abstract::CO(2) partial pressure (pCO(2)) in industrial cell culture reactors may reach 150-200 mm Hg, which can significantly inhibit cell growth and recombinant protein production. The inhibitory effects of elevated pCO(2) at constant pH are due to a combination of the increases in pCO(2) and [HCO(-) (3)], per se, and the ass...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10176
更新日期:2002-02-15 00:00:00
abstract::Surface modified bioactive glass with surface properties akin to those of the bone mineral phase is an attractive candidate for use as a microcarrier material for 3-D growth of bone-like tissue in rotating wall vessel bioreactors (RWVs). The critical surface properties of this material are the result of reaction in so...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10023
更新日期:2001-11-05 00:00:00
abstract::A kinetic model describing the cephalosporin biosynthesis in Streptomyces clavuligerus was developed. Using previously reported kinetic data of biosynthetic enzymes, we examined the kinetics of cephalosporin production. The predicted time profile of the specific production rate during a batch culture parallels that of...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260380815
更新日期:1991-10-20 00:00:00
abstract::The Chinese hamster ovary (CHO) cell line producing interferon-gamma (IFN-gamma) exhibits a 2-fold increase in specific productivity when grown at 32 degrees C compared to 37 degrees C. Low temperature also causes growth arrest, meaning that the cell density is significantly lower at 32 degrees C, nutrients are consum...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10861
更新日期:2004-01-20 00:00:00
abstract::Recently it has been established that low molecular weight displacers can be successfully employed for the purification of proteins in hydrophobic interaction chromatography (HIC) systems. This work investigates the utility of this technique for the purification of an industrial protein mixture. The study involved the...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10582
更新日期:2003-05-05 00:00:00
abstract::A generic "system on a plate" modular multicompartmental bioreactor array which enables microwell protocols to be transferred directly to the bioreactor modules, without redesign of cell culture experiments or protocols is described. The modular bioreactors are simple to assemble and use and can be easily compared wit...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22671
更新日期:2010-05-01 00:00:00
abstract::Vascular leak is a key driver of organ injury in diseases, and strategies that reduce enhanced permeability and vascular inflammation are promising therapeutic targets. Activation of the angiopoietin-1 (ANG1)-Tie2 tyrosine kinase signaling pathway is an important regulator of vascular quiescence. Here we describe the ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27580
更新日期:2021-01-01 00:00:00
abstract::DNA/polyethylenimine (PEI) polyplexes are an important class of nonviral vectors. Although the conventional preparation method, bulk mixing, is straightforward, the formation of the DNA/PEI polyplexes is not well controlled. This work explores coaxial electrohydrodynamic spraying (EHDS) as a novel, alternative method ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22583
更新日期:2010-03-01 00:00:00
abstract::Small interfering RNAs (siRNAs) are invaluable research tools for studying gene functions in mammalian cells. siRNAs are mainly produced by chemical synthesis or by enzymatic digestion of double-stranded RNA (dsRNA) produced in vitro. Recently, bacterial cells, engineered with ectopic plant viral siRNA binding protein...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26740
更新日期:2018-09-01 00:00:00
abstract::Mammalian cell lines for recombinant protein production need to maintain productivity over extended cultivation times. Long-term stability studies are time and resource intensive, but are widely performed to identify and eliminate unstable candidates during cell line development. Production instability of manufacturin...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23216
更新日期:2011-11-01 00:00:00
abstract::Overexpression of the cyclin-dependent kinase inhibitor p27 and exposure to low temperature (30 degrees C) represent two strategies to establish controlled proliferation processes for production of therapeutic proteins using Chinese hamster ovary (CHO) cells. Here we analyze the effect of growth inhibition on the qual...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:2001-03-20 00:00:00
abstract::As photoautotrophic prokaryotes, cyanobacteria are promising platforms for producing value-added bioproducts. However, few regulatory genetic parts and devices (e.g., inducible promoters and regulatory circuits) have been developed for these potential hosts. Furthermore, the devices that have been created respond only...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25722
更新日期:2016-02-01 00:00:00
abstract::Metabolite accumulation has pleiotropic, toxic, or beneficial effects on cell physiology, but such effects are not well understood at the molecular level. Cells respond and adapt to metabolite stress by mechanisms largely unexplored, especially in the context of multiple and simultaneous stresses. Solventogenic and re...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22628
更新日期:2010-04-15 00:00:00