Coping with inadvertent lysis of Escherichia coli cultures: Strains resistant to lysogeny and infection by the stealthy lysogenic phage Φ80.

abstract：:A desirable feature of many therapeutic glycoprotein production processes is to maximize the final sialic acid content. In this study, the effect of applying a novel chemical analog of the sialic acid precursor N-acetylmannosamine (ManNAc) on the sialic acid content of cellular proteins and a model recombinant glycopr...

journal_title：Biotechnology and bioengineering

authors： Yin B,Wang Q,Chung CY,Bhattacharya R,Ren X,Tang J,Yarema KJ,Betenbaugh MJ

更新日期：2017-08-01 00:00:00

abstract：:Regenerative medicine has emerged as an important discipline that aims to repair injury or replace damaged tissues or organs by introducing living cells or functioning tissues. Successful regenerative medicine strategies will likely depend upon a simultaneous optimization strategy for the design of biomaterials, cell-...

journal_title：Biotechnology and bioengineering

authors： Tang Q,Piard C,Lin J,Nan K,Guo T,Caccamese J,Fisher J,Chen Y

更新日期：2018-01-01 00:00:00

abstract：:This article reports a study of the performance of membrane-attached biofilms grown in a single tube extractive membrane bioreactor (STEMS) used for the treatment of a synthetic wastewater containing a toxic VOC (1,2-dichloroethane [DCE]). Mass balances show that complete mineralization of DCE was achieved, and that t...

journal_title：Biotechnology and bioengineering

authors： Dos Santos LM,Livingston AG

更新日期：1995-07-05 00:00:00

abstract：:An approach is presented for the stable covalent immobilization of proteins with a high retention of biological activity. First, chemical modification studies were used to establish enzyme structural and functional properties relevant to the covalent immobilization of an enzyme to agarose based supports. Heparinase wa...

journal_title：Biotechnology and bioengineering

authors： Leckband D,Langer R

更新日期：1991-02-05 00:00:00

abstract：:The production of Bacillus thuringiensis spores was investigated in a bioreactor incorporating a ceramic membrane filter to improve spore concentration and volumetric productivity. Two cultivation methods were used in this study: a total cell retention culture (TCRC), and a two-stage continuous culture with partial ce...

journal_title：Biotechnology and bioengineering

authors： Kang BC,Lee SY,Chang HN

更新日期：1993-11-05 00:00:00

abstract：:We report on a simple method to rapidly generate very large libraries of genes encoding mutant proteins without the use of DNA amplification, and the application of this methodology in the construction of synthetic immunoglobulin variable heavy (V(H)) and light (V(kappa)) libraries. Four high quality, chemically synth...

journal_title：Biotechnology and bioengineering

authors： Ge X,Mazor Y,Hunicke-Smith SP,Ellington AD,Georgiou G

更新日期：2010-06-15 00:00:00

abstract：:Plasmid gene product accumulation in a cell population depends on the fraction of plasmid-containing cells and the distribution of single-cell plasmid content. These important population properties have been related to plasmid replication regulation and kinetics and to plasmid segregation rules at the single-cell leve...

journal_title：Biotechnology and bioengineering

authors： Hjortso MA,Bailey JE

更新日期：1984-05-01 00:00:00

abstract：:Pseudomonads are serious candidates for siderophore production applied to toxic metal (TM) solubilization. The bioaugmentation of contaminated soils by these TM-solubilizing bacteria combined with phytoextraction is an emerging clean-up technology. Unfortunately, siderophore synthesis may be drastically reduced by sol...

journal_title：Biotechnology and bioengineering

authors： Braud A,Jézéquel K,Léger MA,Lebeau T

更新日期：2006-08-20 00:00:00

abstract：:Activation of lignin peroxidase (LIP) in an organic solvent by reversed micelles was investigated. Bis(2-ethylhexyl)sulfosuccinate sodium salt (AOT) was used as a surfactant to form a reversed micelle. Lyophilized LIP from an optimized aqueous solution exhibited no enzymatic activity in any organic solvents examined i...

journal_title：Biotechnology and bioengineering

authors： Kimura M,Michizoe J,Oakazaki SY,Furusaki S,Goto M,Tanaka H,Wariishi H

更新日期：2004-11-20 00:00:00

abstract：:Despite wide applications of l-tyrosine in the market, microbial overproduction of l-tyrosine has been a great challenge due to the complex gene regulations involved in its biosynthetic pathway. To this end, effects of knocking out tyrR on the l-tyrosine production were further explored during the strain development. ...

journal_title：Biotechnology and bioengineering

authors： Kim B,Binkley R,Kim HU,Lee SY

更新日期：2018-10-01 00:00:00

abstract：:A mathematical model is developed to analyze the influence of chemical reaction and diffusion processes on the intracellular organization of mitochondria in skeletal muscle cells. The mathematical modeling approach uses a reaction-diffusion analysis of oxygen, ATP, and ADP involved in energy metabolism and mitochondri...

journal_title：Biotechnology and bioengineering

authors： Pathi B,Kinsey ST,Locke BR

更新日期：2011-08-01 00:00:00

abstract：:Animal cell cultivation in vitro has been studied for more than 40 years. However, the culture medium composition has not been designed on the basis of the stoichiometric nutritional demands for animal cell growth. In this article, a model was developed to study the stoichiometric demands for nutrients (including gluc...

journal_title：Biotechnology and bioengineering

authors： Xie L,Wang DI

更新日期：1994-05-01 00:00:00

abstract：:The effects of ZnCl2 additions on a mercuric reductase, merA, ammonia monooxygenase, amoA, and hydroxylamine (NH2OH) oxidoreductase, hao, gene expression were examined in continuously cultured Nitrosomonas europaea cells. The reactor was operated for 85 days with a 6.9 d hydraulic retention time and with four successi...

journal_title：Biotechnology and bioengineering

authors： Radniecki TS,Semprini L,Dolan ME

更新日期：2009-02-01 00:00:00

abstract：:Intracellular events that take place during influenza virus replication in animal cells are well understood qualitatively. However, to better understand the complex interaction of the virus with its host cell and to quantitatively analyze the use of cellular resources for virion formation or the overall dynamic for th...

journal_title：Biotechnology and bioengineering

authors： Sidorenko Y,Reichl U

更新日期：2004-10-05 00:00:00

abstract：:The methanotrophic bacterium Methylococcus capsulatus is capable of assimilating methane and oxygen into protein-rich biomass, however, the diverse metabolism of the microorganism also allows for several undesired cometabolic side-reactions to occur. In this study, the ammonia cometabolism in Methylococcus capsulatus ...

journal_title：Biotechnology and bioengineering

authors： Petersen LAH,Lieven C,Nandy SK,Villadsen J,Jørgensen SB,Christensen I,Gernaey KV

更新日期：2019-11-01 00:00:00

abstract：:A model for the prediction of protein partition coefficients in aqueous two-phase systems has been developed. This model accounts for both charge-independent and electrostatic effects. The determination of nonelectrostatic effects was based on the model of Eiteman and Gainer for uncharged solutes while the electrostat...

journal_title：Biotechnology and bioengineering

authors： Sebastião MJ,Martel P,Baptista A,Petersen SB,Cabral JM,Aires-Barros MR

更新日期：1997-11-05 00:00:00

abstract：:Hydrophobins are small highly surface-active fungal proteins with potential as biosurfactants in a wide array of applications. However, practical implementation of hydrophobins at large scale has been hindered by low recombinant yields. In this study, the effects of increasing hydrophobin gene copy number and overexpr...

journal_title：Biotechnology and bioengineering

authors： Sallada ND,Harkins LE,Berger BW

更新日期：2019-08-01 00:00:00

abstract：:Alcaligenes Eutrophus H-16 was grown in continuous culture under conditions which induced hydrogenase production. The hydrogenase enzyme was extracted, partially purified and immobilized on porous glass. This enzyme was then studied both in solution and in immobilized form as a possible candidate for a number of indus...

journal_title：Biotechnology and bioengineering

authors： Deloggio TJ,Graves DJ

更新日期：1988-07-20 00:00:00

abstract：:Liquid membrane bilayers, generated by bacteriorhodopsin on a supporting membrane, exhibit photo osmosis. The phenomenon has been shown to be a consequence of light-induced electrical potential differences which develop across the liquid membrane bilayer due to the light-driven proton pumping action of bacteriorhodops...

journal_title：Biotechnology and bioengineering

authors： Srivastava RC,Tandon A

更新日期：1988-04-20 00:00:00

abstract：:Utilization of renewable feedstocks for the production of bio-based chemicals such as d-lactic acid by engineering metabolic pathways in the yeast Saccharomyces cerevisiae has recently become an attractive option. In this study, to realize efficient d-lactic acid production by S. cerevisiae, the expression of 12 glyco...

journal_title：Biotechnology and bioengineering

authors： Yamada R,Wakita K,Mitsui R,Ogino H

更新日期：2017-09-01 00:00:00

abstract：:The thickness variability of biofilms of Pseudomonas aeruginosa, Klebsiella pneumoniae, and the binary population combination of these two species was quantified. The experimental method involved cryoembedding biofilms with a commercial tissue embedding agent, sectioning, and applying image analysis to construct thick...

journal_title：Biotechnology and bioengineering

authors： Murga R,Stewart PS,Daly D

更新日期：1995-03-20 00:00:00

abstract：:The evolution of microbial populations involved in simulated-heap leaching of a polymetallic black schist sulfide ore (from the recently-commissioned Talvivaara mine, Finland) was monitored in aerated packed bed column reactors over a period of 40 weeks. The influence of ore particle size (2-6.5 mm and 6.5-12 mm) on c...

journal_title：Biotechnology and bioengineering

authors： Wakeman K,Auvinen H,Johnson DB

更新日期：2008-11-01 00:00:00

abstract：:Lipase from Candida cylindracea (CCL) was incorporated into polymerizable positively charged dialkylammonium bromide surfactant vesicles. The enzyme was incorporated by the use of the dehydration-rehydration method or by incubation. In the latter case, trapping efficiencies of up to 100% could be obtained. Activities ...

journal_title：Biotechnology and bioengineering

authors： Mosmuller EW,Franssen MC,Engbersen JF

更新日期：1993-06-20 00:00:00

abstract：:The kinetics of cellular reproduction and the rate and extent of synthesis of extracellular polymeric substances (EPS) were investigated for P. aeruginosa growing in glucose-limited chemostats. mu(max) and K(s) estimates of 0.4 h(-1) and 2 mg glucose C/L, respectively, at 25 degrees C were obtained for this bacterium....

journal_title：Biotechnology and bioengineering

authors： Robinson JA,Trulear MG,Characklis WG

更新日期：1984-12-01 00:00:00

abstract：:The stoichiometry and kinetics of the spontaneous, chemical reaction between pyrite and ferric iron was studied at 30, 45, and 70 degrees C in shake flasks at pH 1.5 by monitoring the ferrous iron, total iron, elemental sulfur, and sulfate concentration profiles in time. It was found that the sulfur moiety of pyrite w...

journal_title：Biotechnology and bioengineering

authors： Boogerd FC,van den Beemd C,Stoelwinder T,Bos P,Kuenen JG

更新日期：1991-06-20 00:00:00

abstract：:Peroxidase-catalyzed asymmetric sulfoxidations, while synthetically attractive, suffer from relatively low reaction rates due to poor substrate solubilities in water and from appreciable spontaneous oxidation of substrates (especially aryl alkyl sulfides) with H(2)O(2). In this work, we found that both of these shortc...

journal_title：Biotechnology and bioengineering

authors： Dai L,Klibanov AM

更新日期：2000-11-05 00:00:00

abstract：:Dielectric spectroscopy was applied to two industrial high cell density culture processes and used to determine on-line the concentration of CHO cells immobilized on macroporous microcarriers in a stirred bioreactor and in a packed-bed of disk carriers. The cell concentration predicted from the spectroscopic data was ...

journal_title：Biotechnology and bioengineering

authors： Ducommun P,Kadouri A,von Stockar U,Marison IW

更新日期：2002-02-05 00:00:00

abstract：:The reaction kinetics for phenol biodegradation at low substrate concentrations can be estimated based on the analysis of changes in the dissolved oxygen concentration in the bulk liquid during biodegradation. The measured oxygen concentration changes with an interesting behavior as biodegradation proceeds. The oxygen...

journal_title：Biotechnology and bioengineering

authors： Shishido M,Toda M

更新日期：1996-06-20 00:00:00

abstract：:Continuous virus inactivation (VI) has received little attention in the efforts to realize fully continuous biomanufacturing in the future. Implementation of continuous VI must assure a specific minimum incubation time, typically 60 min. To guarantee the minimum incubation time, we implemented a packed bed continuous ...

journal_title：Biotechnology and bioengineering

authors： Martins DL,Sencar J,Hammerschmidt N,Flicker A,Kindermann J,Kreil TR,Jungbauer A

更新日期：2020-05-01 00:00:00

abstract：:Conventional wet chemistry methods to determine biomass composition are labor- and time-intensive and require larger amounts of biomass (300 mg) than is often available. To overcome these limitations and to support a high-throughput pretreatment and hydrolysis (HTPH) screening system, this article reports on the devel...

journal_title：Biotechnology and bioengineering

authors： DeMartini JD,Studer MH,Wyman CE

更新日期：2011-02-01 00:00:00