Apparent zero-order kinetics of phenol biodegradation by substrate-inhibited microbes at low substrate concentrations.

abstract：:The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that constitutive secretion...

journal_title：Biotechnology and bioengineering

authors： Love KR,Panagiotou V,Jiang B,Stadheim TA,Love JC

更新日期：2010-06-01 00:00:00

abstract：:An acetate-rich wastewater, containing 170 mg/L of total organic carbon (TOC), 13 mg/L of N, and 15 mg/L of P, was treated using the enhanced biological phosphate removal (EBPR) process operated in a sequencing batch reactor. A slight change of pH of the mixed liquor from 7.0 to 6.5 led to a complete loss of phosphate...

journal_title：Biotechnology and bioengineering

authors： Zhang T,Liu Y,Fang HH

更新日期：2005-10-20 00:00:00

abstract：:The mixed-culture anaerobic conversion of lactose to organic acids in a bench-scale continuous-flow stirredtank fermentor is considered. The major acidogenic end-product distribution with respect to the dilution rate are presented. A Monod chemostat model is employed to describe a microbial growth, and the influence o...

journal_title：Biotechnology and bioengineering

authors： Kissalita WS,Lo KV,Pinder KL

更新日期：1989-01-25 00:00:00

abstract：:Relative levels of many individual proteins in Escherichia coli HB101 strains with 0, 37, 56, and 240 plasmids per chromosome were determined by computer image analysis of two-dimensional gel electrophoresis patterns. The plasmids investigated had very similar sequences except for small domains encoding the repressor ...

journal_title：Biotechnology and bioengineering

authors： Birnbaum S,Bailey JE

更新日期：1991-04-05 00:00:00

abstract：:A novel technique for immobilization of Candida rugosa lipase onto anionic colloidal gas aphrons (CGAs) is described. CGAs are spherical microbubbles (10-100 microm) composed of an inner gas core surrounded by a surfactant shell. In this initial study, greater than 80% lipase (w/w) was effectively retained on the CGAs...

journal_title：Biotechnology and bioengineering

authors： O'Connell PJ,Varley J

更新日期：2001-08-05 00:00:00

abstract：:During continuous lipase-catalyzed oleic acid esterification by ethanol in n-hexane, the oleic acid conversion, initially at 95%, decreases to 20% after 2 h. This decrease is caused by the accumulation of the water produced in the course of the reaction in the packed-bed reactor (PBR). In order to improve the PBR effi...

journal_title：Biotechnology and bioengineering

authors： Colombié S,Tweddell RJ,Condoret JS,Marty A

更新日期：1998-11-05 00:00:00

abstract：:Seven different supports were compared in solid-phase S(N)Ar and S(N)2 macrocyclization reactions. Product purities were assayed for a relatively facile ring-closure process to give products 1 and 3. Some less-facile ring-closure reactions give the undesired dimeric macrocyclization by-products 2; some of these more-d...

journal_title：Biotechnology and bioengineering

authors： Feng Y,Burgess K

更新日期：2000-01-01 00:00:00

abstract：:Continuous virus inactivation (VI) has received little attention in the efforts to realize fully continuous biomanufacturing in the future. Implementation of continuous VI must assure a specific minimum incubation time, typically 60 min. To guarantee the minimum incubation time, we implemented a packed bed continuous ...

journal_title：Biotechnology and bioengineering

authors： Martins DL,Sencar J,Hammerschmidt N,Flicker A,Kindermann J,Kreil TR,Jungbauer A

更新日期：2020-05-01 00:00:00

abstract：:A modified simulation procedure based on a statistical approach was investigated. The procedure predicts the time course of fed-batch culture for glutamic acid production by a temperature-sensitive strain of Brevibacterium flavum. The statistical approach requires only a data base of state points obtained in experimen...

journal_title：Biotechnology and bioengineering

authors： Kishimoto M,Alfafara CG,Nakajima M,Yoshida T,Taguchi H

更新日期：1989-01-10 00:00:00

abstract：:The mouse mammary tumor virus (MMTV) promoter is induced by the addition of a glucocorticoid hormone or analog such as dexamethasone. The hormone binds to its specific transcription factor, glucocorticoid receptor (GR), and the activated complex then binds to the glucocorticoid response elements (GREs) in the enhancer...

journal_title：Biotechnology and bioengineering

authors： James RI,Elton JP,Todd P,Kompala DS

更新日期：2000-01-20 00:00:00

abstract：:The possibility of biologically detoxifying a contaminated soil from an Italian dump site containing about 1500 mg/kg (in dry soil) of polychlorinated biphenyls was studied in the laboratory in this work. The soil, which contained indigenous aerobic bacteria capable of growing on biphenyl or on monochlorobenzoic acids...

journal_title：Biotechnology and bioengineering

authors： Fava F,Bertin L

更新日期：1999-07-20 00:00:00

abstract：:An approach is presented for the stable covalent immobilization of proteins with a high retention of biological activity. First, chemical modification studies were used to establish enzyme structural and functional properties relevant to the covalent immobilization of an enzyme to agarose based supports. Heparinase wa...

journal_title：Biotechnology and bioengineering

authors： Leckband D,Langer R

更新日期：1991-02-05 00:00:00

abstract：:The long hydrocarbon fatty acyl chain is energy rich, making it an ideal precursor for liquid transportation fuels and high-value oleo chemicals. As Saccharomyces cerevisiae has many advantages for industrial production compared to Escherichia coli. Here, we attempted to engineer Saccharomyces cerevisiae for overprodu...

journal_title：Biotechnology and bioengineering

authors： Li X,Guo D,Cheng Y,Zhu F,Deng Z,Liu T

更新日期：2014-09-01 00:00:00

abstract：:Phosphite dehydrogenase represents a new enzymatic system for regenerating reduced nicotinamide cofactors for industrial biocatalysis. We previously engineered a variant of phosphite dehydrogenase with relaxed cofactor specificity and significantly increased activity and stability. Here we performed one round of rando...

journal_title：Biotechnology and bioengineering

authors： McLachlan MJ,Johannes TW,Zhao H

更新日期：2008-02-01 00:00:00

abstract：:In vivo mesenchymal stem cell (MSC) survival is relevant to therapeutic applications requiring engraftment and potentially to nonengraftment applications as well. MSCs are a mixture of progenitors at different stages of cellular aging, but the contribution of this heterogeneity to the survival of MSC implants is unkno...

journal_title：Biotechnology and bioengineering

authors： Madsen SD,Jones SH,Tucker HA,Giler MK,Muller DC,Discher CT,Russell KC,Dobek GL,Sammarco MC,Bunnell BA,O'Connor KC

更新日期：2020-01-01 00:00:00

abstract：:The DeltaSf of one unit carbon formula weight of Escherichia coli K-12 cells, when grown on succinic acid, was calculated to be -80.13 J/deg. This value could then be used to calculate the entropy change accompanying the anabolism and metabolism of succinic acid to be 30.82 J/deg and 32.40 J/mol deg, respectively. The...

journal_title：Biotechnology and bioengineering

authors： Battley EH

更新日期：1993-02-20 00:00:00

abstract：:Extracellular beta-xylosidase (1,4-beta-D-xylan xylohydrolase, EC 3.2.1.37) from culture filtrates of Neurospora crassa was purified to homogeneity by preparative isoelectric focusing followed by gel electrophoresis. The molecular weight of the purified xylosidase was 83,000 D and the K(m) on p-nitrophenyl-beta-D-xylo...

journal_title：Biotechnology and bioengineering

authors： Deshpande V,Lachke A,Mishra C,Keskar S,Rao M

更新日期：1986-12-01 00:00:00

abstract：:Equilibrium adsorption studies on Escherichia coli and Saccharomyces sp. revealed the capacity and affinity of these organisms for the surfaces of powdered charcoal and nickel. In simple salt solutions both organisms readily adsorbed to each solid with an affinity and maximum loading capacity individual to each cell-s...

journal_title：Biotechnology and bioengineering

authors： Protheroe RG,Cumming RH,Matchett A

更新日期：1989-10-05 00:00:00

abstract：:Screening tests of different marine algae biomas types revealed a high passive biosorptive uptake of lead up to 270 mg Pb/g of biomass in some brown marine algae. Members of the order Fucales perfomed particularly well in this descending sequence: Fucus > Ascophyllum > Sargassum. Although decreasing the swelling of we...

journal_title：Biotechnology and bioengineering

authors： Holan ZR,Volesky B

更新日期：1994-05-01 00:00:00

abstract：:Olive oil hydrolysis using Chromobacterium viscosum lipase B in a reversed micellar media was investigated in a membrane reactor. The dynamic evolution of the product concentration both in the concentrate and permeate stream was analyzed using a mechanistic model previously developed by us and further modified in this...

journal_title：Biotechnology and bioengineering

authors： Prazeres DM,Lemos F,Garcia FA,Cabral JM

更新日期：1993-09-05 00:00:00

abstract：:A flow cytometric kinetic study of hybridoma growth and monoclonal antibody production is presented, along with the influence of glutamine on intracellular responses such as (relative) cell size, and cell RNA and total protein content. Specific findings are: (1) RNA content remained constant throughout the growth phas...

journal_title：Biotechnology and bioengineering

authors： Dalili M,Ollis DF

更新日期：1990-06-05 00:00:00

abstract：:The attrition bioreactor (ABR) combines wet ball milling and enzymatic hydrolysis in one process step. It was found that the ABR did not accelerate enzyme deactivation. Interfacial forces, not shear forces, caused the most deactivation. Elimination of the air-liquid interface by covering the reactor substantially incr...

journal_title：Biotechnology and bioengineering

authors： Jones EO,Lee JM

更新日期：1988-01-01 00:00:00

abstract：:The adsorption of cellulase on cellulose and a lignacious residue was examined by using cellulase from Trichoderma reesei, hardwood pretreated by dilute sulfuric acid under high pressure, and a lignacious residue prepared by a complete enzymatic hydrolysis of the pretreated wood. A significant amount of cellulase was ...

journal_title：Biotechnology and bioengineering

authors： Ooshima H,Burns DS,Converse AO

更新日期：1990-08-20 00:00:00

abstract：:The specific uptake rate of phenol by washed cells of Pseudomonas putida grown on phenol in steady-state continuous culture at various dilution rates was studied. The Monod-Haldane-type equation was applied to fit the data and the best kinetic parameters were determined by nonlinear least-squares techniques. The value...

journal_title：Biotechnology and bioengineering

authors： Sokol W

更新日期：1987-12-05 00:00:00

abstract：:Enzymatic hydrolysis of arabinoxylan is an important prerequisite for the utilization of hemicellulose for ethanol fermentation or for making the low calorie sweetener xylitol by catalytic hydrogenation of the generated xylose. This study focus on cloning and characterization of two industrial relevant beta-xylosidase...

journal_title：Biotechnology and bioengineering

authors： Rasmussen LE,Sørensen HR,Vind J,Viksø-Nielsen A

更新日期：2006-08-05 00:00:00

abstract：:The scope in improving enzyme productivities from the cellulose fermentation process is examined in laboratory-scale fermentors. The maximum productivity (30 IU/liter hr) is attained in a continuous-culture process with cell recycle using modified medium containing 0.5% cellulose. Optimum dilution rate and recycle rat...

journal_title：Biotechnology and bioengineering

authors： Ghose TK,Sahai V

更新日期：1979-02-01 00:00:00

abstract：:Gluconic acid and sorbitol were simultaneously produced from glucose and Jerusalem artichoke using a glucose-fructose oxidoreductase of Zymomonas mobilis and inulinase. Inulinase was immobilized on chitin by cross-linking with glutaraldehyde. Cells of Z. mobilis permeabilized with toluene were coimmobilized with chiti...

journal_title：Biotechnology and bioengineering

authors： Kim DM,Kim HS

更新日期：1992-02-05 00:00:00

abstract：:In order to eliminate organic pollutants in waste gases, a biological filter bed technique has been developed, with a high self-regenerating capacity and a low pressure drop. The bed consists of an appropriate filling material (mainly peat compost), in order to let the microorganisms grow on the solid surface and to s...

journal_title：Biotechnology and bioengineering

authors： Ottengraf SP,Van Den Oever AH

更新日期：1983-12-01 00:00:00

abstract：:Insulin is an important biotherapeutic protein, and it is also a model protein used to study amyloid diseases, such as Alzheimer's and Parkinson's. The preparation of the protein can lead to small amounts of aggregate in the solution, which in turn may lead to irreproducible in vitro results. Using several pre-treatme...

journal_title：Biotechnology and bioengineering

authors： Heldt CL,Sorci M,Posada D,Hirsa A,Belfort G

更新日期：2011-01-01 00:00:00

abstract：:Intrinsic models, which take into account biomass volume fraction, must be formulated for adequate simulation of high-biomass-density fermentations with cell recycle. Through comparison of corresponding intrinsic and non-intrinsic models in dimensionless form, constraints for non-intrinsic model usage in terms of biok...

journal_title：Biotechnology and bioengineering

authors： Monbouquette HG

更新日期：1987-06-01 00:00:00