Mode of action and properties of xylanase and beta-Xylosidase from Neurospora crassa.

abstract：:In the process of producing ethanol from lignocellulosic materials such as wheat straw, compounds that can act inhibitory to enzymatic hydrolysis and to cellular growth may be generated during the pretreatment. Ethanol production was evaluated on pretreated wheat straw hydrolysate using four different recombinant Sacc...

journal_title：Biotechnology and bioengineering

authors： Panagiotou G,Olsson L

更新日期：2007-02-01 00:00:00

abstract：:The scope in improving enzyme productivities from the cellulose fermentation process is examined in laboratory-scale fermentors. The maximum productivity (30 IU/liter hr) is attained in a continuous-culture process with cell recycle using modified medium containing 0.5% cellulose. Optimum dilution rate and recycle rat...

journal_title：Biotechnology and bioengineering

authors： Ghose TK,Sahai V

更新日期：1979-02-01 00:00:00

abstract：:The effects of dilution rates on the performance of a two-stage fermentation system for a recombinant Escherichia coli culture were studied. Dilution rate determines the apparent or averaged specific growth rate of a heterogeneous population of cells in the recombinant culture. The specific growth rate affects the gen...

journal_title：Biotechnology and bioengineering

authors： Hortacsu A,Ryu DD

更新日期：1991-10-20 00:00:00

abstract：:Green fluorescent protein (GFP) was used to study the regulation of the galactose-inducible GAL1 promoter in yeast Saccharomyces cerevisiae strains. GFP was cloned into the pGAL110 vector and transformed into the yeast strains. Time course studies comparing culture fluorescence intensity and GFP concentration were con...

journal_title：Biotechnology and bioengineering

authors： Li J,Wang S,VanDusen WJ,Schultz LD,George HA,Herber WK,Chae HJ,Bentley WE,Rao G

更新日期：2000-10-20 00:00:00

abstract：:The addition of simple inorganic salts to aqueous enzyme solutions prior to lyophilization results in a dramatic activation of the dried powder in organic media relative to enzyme with no added salt. Activation of both the serine protease subtilisin Carlsberg and lipase from Mucor javanicus resulting from lyophilizati...

journal_title：Biotechnology and bioengineering

authors： Ru MT,Dordick JS,Reimer JA,Clark DS

更新日期：1999-04-20 00:00:00

abstract：:In glutamic acid fermentation, the molasses feeding policy and time of penicillin addition significantly affected glutamic acid production, and a fuzzy supervisory control system was developed for their quasi-optimal regulation.From the trend of the experimental data, production rules and membership functions of fuzzy...

journal_title：Biotechnology and bioengineering

authors： Kitsuta Y,Kishimoto M

更新日期：1994-06-05 00:00:00

abstract：:Cobalt chromium (CoCr) alloy is widely used in orthopedic implants but its functional longevity is susceptible to inflammation related complications. Reduction of the development of chronic inflammation on the biomaterial surface would enhance direct bone-implant bonding and improve implant survival and long-term resu...

journal_title：Biotechnology and bioengineering

authors： Tan HC,Poh CK,Cai Y,Soe MT,Wang W

更新日期：2013-03-01 00:00:00

abstract：:Intrinsic models, which take into account biomass volume fraction, must be formulated for adequate simulation of high-biomass-density fermentations with cell recycle. Through comparison of corresponding intrinsic and non-intrinsic models in dimensionless form, constraints for non-intrinsic model usage in terms of biok...

journal_title：Biotechnology and bioengineering

authors： Monbouquette HG

更新日期：1987-06-01 00:00:00

abstract：:We developed a self-assembling polymer based on polyallylamine (PAH) for use in DNA chips. Thioctic acid (TA) was covalently attached to PAH in sidechains to immobilize the polymer on a gold surface by self-assembly. N-hydroxysuccinimide-ester terminated probe single-stranded (ss) DNA is easily covalently immobilized ...

journal_title：Biotechnology and bioengineering

authors： Taira S,Yokoyama K

更新日期：2005-03-30 00:00:00

abstract：:Remarkable increases in enzyme catalytic stability resulting from addition of charged water-soluble polymers have recently been reported, suggesting that use of these polymers may be an attractive general strategy for enzyme stabilization. To test the proposed hypothesis that coulombic forces between water-soluble pol...

journal_title：Biotechnology and bioengineering

authors： Foreman TM,Khalil M,Meier P,Brainard JR,Vanderberg LA,Sauer NN

更新日期：2001-11-01 00:00:00

abstract：:A highly active xylose specific transporter without glucose inhibition is highly desirable in cost-effective production of biofuels from lignocellulosic biomass. However, currently available xylose specific transporters suffer from low overall activity and most are inhibited by glucose. In this study, we applied a dir...

journal_title：Biotechnology and bioengineering

authors： Wang M,Yu C,Zhao H

更新日期：2016-03-01 00:00:00

abstract：:Urea, ammonium, and free amino acid contents were quantified in biological aging of a young wine under two flor film forming yeast strains, Saccharomyces cerevisiae race capensis and S. cerevisiae race bayanus, and compared. Cell viability in the film was different for the two yeast strains. Thus, capensis maintained ...

journal_title：Biotechnology and bioengineering

authors： Mauricio JC,Ortega JM

更新日期：1997-01-20 00:00:00

abstract：:The effect of the water content on the lipase-catalyzed (Candida rugosa) interesterification (acidolysis) of menhaden oil with conjugated linoleic acid was studied for amounts of added water ranging from 0-4% (w/w). The rate of the acidolysis reaction increased with increasing water content, but the corresponding perc...

journal_title：Biotechnology and bioengineering

authors： Torres CF,Hill CG Jr

更新日期：2002-06-05 00:00:00

abstract：:We propose that transcript levels for some genes are affected by the bacterial cell division cycle and this may be an important factor to consider when designing synthetic bacterial genomes. To test this hypothesis, transcript levels of 58 genes in Escherichia coli B/r A were determined at five times during the cell d...

journal_title：Biotechnology and bioengineering

authors： Echtenkamp PL,Wilson DB,Shuler ML

更新日期：2009-02-15 00:00:00

abstract：:A novel endoglucanase gene, celM , was cloned from a thermal spring metagenome. The gene was expressed in Escherichia coli, and the protein was extracted and purified. The protein catalyzed the hydrolysis of amorphous cellulose in a wide range of temperatures, 30-95°C, with optimal activity at 80°C. It was able to tol...

journal_title：Biotechnology and bioengineering

authors： Joshi N,Kaushal G,Singh SP

更新日期：2021-01-06 00:00:00

abstract：:Nutrient utilization and byproduct accumulation were monitored in Spodoptera frugiperda Sf-9 and Trichoplusia ni BTI-Tn-5B1-4 (High Five) cell lines during growth and following viral infection in suspension cultures in order to develop a better understanding of cell metabolism and to acquire information relevant to la...

journal_title：Biotechnology and bioengineering

authors： Rhiel M,Mitchell-Logean CM,Murhammer DW

更新日期：1997-09-20 00:00:00

abstract：:Overexpression of the cyclin-dependent kinase inhibitor p27 and exposure to low temperature (30 degrees C) represent two strategies to establish controlled proliferation processes for production of therapeutic proteins using Chinese hamster ovary (CHO) cells. Here we analyze the effect of growth inhibition on the qual...

journal_title：Biotechnology and bioengineering

authors： Kaufmann H,Mazur X,Marone R,Bailey JE,Fussenegger M

更新日期：2001-03-20 00:00:00

abstract：:CTLA4 is a membrane receptor on cytotoxic T cells whose interaction with the B7 counterreceptor on B cells is important in alloantigen responses. Soluble recombinant human and murine CTLA4 were produced using either Chinese hamster ovary or NS-0 cell lines. Expression vectors were constructed containing the gene codin...

journal_title：Biotechnology and bioengineering

authors： Flesher AR,Marzowski J,Wang WC,Raff HV

更新日期：1995-06-05 00:00:00

abstract：:Aryl-alcohol oxidase (AAO) plays a fundamental role in the fungal ligninolytic secretome, acting as a supplier of H2 O2 . Despite its highly selective mechanism of action, the presence of this flavooxidase in different biotechnological settings has hitherto been hampered by the lack of appropriate heterologous express...

journal_title：Biotechnology and bioengineering

authors： Viña-Gonzalez J,Elbl K,Ponte X,Valero F,Alcalde M

更新日期：2018-07-01 00:00:00

abstract：:Glycosylation is a key critical quality attribute for monoclonal antibodies and other recombinant proteins because of its impact on effector mechanisms and half-life. In this study, a variety of compounds were evaluated for their ability to modulate glycosylation profiles of recombinant monoclonal antibodies produced ...

journal_title：Biotechnology and bioengineering

authors： Ehret J,Zimmermann M,Eichhorn T,Zimmer A

更新日期：2019-04-01 00:00:00

abstract：:A procedure for measuring the rate of heat production from a fermentation has been developed. The method is based on measuring the rate of temperature rise of the fermentation broth resulting from metabolism, when the temperature controller is turned off. The heat accumulation measured in this manner is then corrected...

journal_title：Biotechnology and bioengineering

authors： Cooney CL,Wang DI,Mateles RI

更新日期：2000-03-20 00:00:00

abstract：:In this study, the inhibitory effects and anaerobic biotransformation of acrylic acid in computer-controlled pH-stat completely stirred tank reactors (CSTRs) with two different cultures, namely unacclimated and acrylate-acclimated acetate-enriched Methanosarcina and homogenized (crushed) granular cultures, were invest...

journal_title：Biotechnology and bioengineering

authors： Demirer GN,Speece RE

更新日期：1999-01-20 00:00:00

abstract：:Mass transfer is known to play a critical role in bioprocess performance and henceforth monitoring dissolved O2 (DO) and dissolved CO2 (dCO2 ) is of paramount importance. At bioreactor level these parameters can be monitored online and can be controlled by sparging air/oxygen or stirrer speed. However, traditional sma...

journal_title：Biotechnology and bioengineering

authors： Chopda VR,Holzberg T,Ge X,Folio B,Wong L,Tolosa M,Kostov Y,Tolosa L,Rao G

更新日期：2020-04-01 00:00:00

abstract：:We have shown previously that a sequence-specific DNA-binding protein based on the Lac repressor protein can isolate pre-purified DNA efficiently from simple buffer solution but our attempts to purify plasmids directly from crude starting materials were disappointing with impractically low DNA yields. We have optimize...

journal_title：Biotechnology and bioengineering

authors： Darby RA,Forde GM,Slater NK,Hine AV

更新日期：2007-12-01 00:00:00

abstract：:The development of renewable alternatives to diesel and jet fuels is highly desirable for the heavy transportation sector, and would offer benefits over the production and use of short-chain alcohols for personal transportation. Here, we report the development of a metabolically engineered strain of Escherichia coli t...

journal_title：Biotechnology and bioengineering

authors： Lennen RM,Braden DJ,West RA,Dumesic JA,Pfleger BF

更新日期：2010-06-01 00:00:00

abstract：:For in vitro liver replacement devices, such as packed bed bioreactors, to maintain the essential functions of the liver, they must at least successfully support hepatocytes, the parenchymal cell of the liver. In vivo, the liver is a major consumer of oxygen. Hence it is unsurprising that the limited transport distanc...

journal_title：Biotechnology and bioengineering

authors： Niu M,Clemens MG,Coger RN

更新日期：2008-04-15 00:00:00

abstract：:The first genome scale model (GSM) for Streptomyces leeuwenhoekii C34 was developed to study the biosynthesis pathways of specialized metabolites and to find metabolic engineering targets for enhancing their production. The model, iVR1007, consists of 1,722 reactions, 1,463 metabolites, and 1,007 genes, it includes th...

journal_title：Biotechnology and bioengineering

authors： Razmilic V,Castro JF,Andrews B,Asenjo JA

更新日期：2018-07-01 00:00:00

abstract：:The study was done to evaluate the efficacy of using reuterin produced by Lactobacillus reuteri to sanitize biological tissues. The microorganism tested in the study was Pseudomonas aeruginosa, a common cause of nosocomial biomaterial-related infections. The inhibitory effect of reuterin on P. aeruginosa for an inocul...

journal_title：Biotechnology and bioengineering

authors： Liang HF,Chen CN,Chang Y,Sung HW

更新日期：2003-10-20 00:00:00

abstract：:Linear relations among net conversion rates of primary metabolites in fermentation processes can be determined by using the macroscopic or pathway balancing methods. It is shown that the process observability can be improved by incorporating both the abiotic proton balance and the pH control reagent addition rate meas...

journal_title：Biotechnology and bioengineering

authors： Siano SA

更新日期：1996-12-20 00:00:00

abstract：:A metabolic shift from lactate production (LP) to net lactate consumption (LC) phenotype was observed in certain Chinese hamster ovary (CHO) cell lines during the implementation of a new chemically defined medium (CDM) formulation for antibody production. In addition, this metabolic shift typically leads to process pe...

journal_title：Biotechnology and bioengineering

authors： Luo J,Vijayasankaran N,Autsen J,Santuray R,Hudson T,Amanullah A,Li F

更新日期：2012-01-01 00:00:00