Abstract:
:Intrinsic models, which take into account biomass volume fraction, must be formulated for adequate simulation of high-biomass-density fermentations with cell recycle. Through comparison of corresponding intrinsic and non-intrinsic models in dimensionless form, constraints for non-intrinsic model usage in terms of biokinetic and fermenter operating parameters can be identified a priori. Analysis of a simple product-inhibition model indicates that the non-intrinsic approach is suitable only when the attainable biomass volume fraction in the fermentation broth is less than about 0.10. Inappropriate application of a non-intrinsic model can lead to gross errors in calculated substrate and product concentrations, substrate conversion, and volumetric productivity.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Monbouquette HGdoi
10.1002/bit.260290906subject
Has Abstractpub_date
1987-06-01 00:00:00pages
1075-80issue
9eissn
0006-3592issn
1097-0290journal_volume
29pub_type
杂志文章abstract::The downstream processing of virus particles for vaccination or gene therapy is becoming a critical bottleneck as upstream titers keep improving. Moreover, the growing pressure to develop cost-efficient processes has brought forward new downstream trains. This review aims at analyzing the state-of-the-art in viral dow...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章,评审
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更新日期:2015-05-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24551
更新日期:2012-11-01 00:00:00
abstract::Analytical expressions describing convective flow in a continuous arteriovenous hollow fiber hemofilter were developed. In the lumen of the hollow fiber membrane, existing analytical expressions were applied to describe velocity profiles and pressure. For flow in the shell (the extracapillary space separating the fibe...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2000-04-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260370711
更新日期:1991-03-25 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260430613
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1044
更新日期:2001-04-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270407
更新日期:1985-04-01 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:2018-12-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2019-09-01 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:2003-12-05 00:00:00
abstract::Intracellular delivery of functional proteins is of great interest for basic biological research as well as for clinical applications. Transfection is the most commonly used method, however, it is not applicable to large-scale manipulation and inefficient in important cell types implicated in biomedical applications, ...
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1999-06-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260241105
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journal_title:Biotechnology and bioengineering
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更新日期:2019-01-01 00:00:00