Countercurrent multistage fluidized bed reactor for immobilized biocatalysts: I. Modeling and simulation.

abstract：:On-line NAD(P)H fluorescence and culture redox potential (CRP) measurements were utilized to investigate the role of Vitreoscilla hemoglobin (VHb) in perturbing oxygen metabolism of microaerobic Escherichia coli Batch cultures of a VHb-synthesizing E. coli strain and the iso-genic control under fully aerated condition...

journal_title：Biotechnology and bioengineering

authors： Tsai PS,Rao G,Bailey JE

更新日期：1995-08-05 00:00:00

abstract：:The kinetic properties of collagen-bound firefly luciferase have been investigated. Under definite hydrodynamic conditions with low agitation in the reaction medium, the observed behavior is modified compared to the enzyme free in solution: reducing the stirring rate decreases the observed enzymatic activity. But diff...

journal_title：Biotechnology and bioengineering

authors： Blum LJ,Coulet PR

更新日期：1986-08-01 00:00:00

abstract：:Recent studies have demonstrated the feasibility of using ultrafiltration for the purification of plasmid DNA, but there is still little understanding of the factors governing DNA transmission. Experimental data were obtained for the transmission of a 3.0 kbp supercoiled plasmid DNA through composite regenerated cellu...

journal_title：Biotechnology and bioengineering

authors： Latulippe DR,Zydney AL

更新日期：2008-02-01 00:00:00

abstract：:The antibiotic, difficidin, and its hydroxylated derivative oxydifficidin, were synthesized by cultures of Bacillus subtilis grown on a complex medium. Maximum titers of about 200 and 130 mg/L, respectively, were obtained. In fermentations where the dissolved oxygen tension (DOT) was controlled, the maximum specific g...

journal_title：Biotechnology and bioengineering

authors： Suphantharika M,Ison AP,Lilly MD,Buckland BC

更新日期：1994-10-01 00:00:00

abstract：:Nucleotide sugar-dependent ("Leloir") glycosyltransferases (GTs), represent a new paradigm for the application of biocatalytic glycosylations to the production of fine chemicals. However, it remains to be shown that GT processes meet the high efficiency targets of industrial biotransformations. We demonstrate in this ...

journal_title：Biotechnology and bioengineering

authors： Schmölzer K,Lemmerer M,Gutmann A,Nidetzky B

更新日期：2017-04-01 00:00:00

abstract：:The quality of cassava starch, an important trait in cassava breeding programs, determines its applications in various industries. For example, development of waxy (having a low level of amylose) cassava is in demand. Amylose is synthesized by granule-bound starch synthase I (GBSSI) in plants, and therefore, down-regu...

journal_title：Biotechnology and bioengineering

authors： Zhao SS,Dufour D,Sánchez T,Ceballos H,Zhang P

更新日期：2011-08-01 00:00:00

abstract：:Enzymatic acrylation is a process of potentially strong interest to the chemical industry. Direct esterification involving acrylic acid is unfortunately rather slow, with inhibition phenomena appearing at high acid concentrations. In the present study the acrylation of 1-octanol catalyzed by immobilized Candida antarc...

journal_title：Biotechnology and bioengineering

authors： Nordblad M,Adlercreutz P

更新日期：2008-04-15 00:00:00

abstract：:The lignin peroxidases (LIP) and manganese peroxidases (MNP) of Phanerochaete chrysosporium catalyze a wide range of lignin depolymerization reactions with lignin models and synthetic lignins in solution. However, their ability to degrade insoluble natural lignin in aqueous media has not been demonstrated. Insoluble i...

journal_title：Biotechnology and bioengineering

authors： Thompson DN,Hames BR,Reddy CA,Grethlein HE

更新日期：1998-03-20 00:00:00

abstract：:Buffered aqueous two-phase systems are effective extraction systems for separating amphoteric hydrocarbons like, for example, polypeptides from aqueous phases. The design and basic engineering of such processes requires the knowledge of the liquid-liquid equilibrium. The study presented here aims to contribute to the ...

journal_title：Biotechnology and bioengineering

authors： Grossmann C,Tintinger R,Zhu J,Maurer G

更新日期：1998-12-20 00:00:00

abstract：:Anoxic and metabolic stresses in large-scale cell culture during biopharmaceutical production can induce apoptosis. Strategies designed to ameliorate the problem of apoptosis in cell culture have focused on mRNA knockdown of pro-apoptotic proteins and over-expression of anti-apoptotic ones. Apoptosis in cell culture i...

journal_title：Biotechnology and bioengineering

authors： Cost GJ,Freyvert Y,Vafiadis A,Santiago Y,Miller JC,Rebar E,Collingwood TN,Snowden A,Gregory PD

更新日期：2010-02-01 00:00:00

abstract：:The glycosylation of pharmaglycoproteins from recombinant cell lines can be affected by an uncontrolled accumulation of ammonium in the medium. Glucosamine-6-phosphate isomerase (GPI) has been proposed as the key enzyme responsible for elevating the intracellular UDP-N-acetylhexosamine pool (UDPGNAc) by accepting ammo...

journal_title：Biotechnology and bioengineering

authors： Cayli A,Hirschmann F,Wirth M,Hauser H,Wagner R

更新日期：1999-10-20 00:00:00

abstract：:Utilization of renewable feedstocks for the production of bio-based chemicals such as d-lactic acid by engineering metabolic pathways in the yeast Saccharomyces cerevisiae has recently become an attractive option. In this study, to realize efficient d-lactic acid production by S. cerevisiae, the expression of 12 glyco...

journal_title：Biotechnology and bioengineering

authors： Yamada R,Wakita K,Mitsui R,Ogino H

更新日期：2017-09-01 00:00:00

abstract：:Experimental observations indicate that the rates of cometabolic transformation are linked to the consumption of growth substrate during growth and to the consumption of cell mass and/or energy substrate in the absence of growth substrate. Three previously proposed models (models 1 through 3) describing the kinetics o...

journal_title：Biotechnology and bioengineering

authors： Criddle CS

更新日期：1993-05-01 00:00:00

abstract：:Five microbial inocula were evaluated in batch tests for the ability to remediate mine drainage (MD). Dairy manure (DM), anaerobic digester sludge, substrate from the Luttrell (LUTR) and Peerless Jenny King (PJK) sulfate-reducing permeable reactive zones (SR-PRZs) and material from an MD-treatment column that had been...

journal_title：Biotechnology and bioengineering

authors： Pereyra LP,Hiibel SR,Pruden A,Reardon KF

更新日期：2008-11-01 00:00:00

abstract：:We have shown previously that a sequence-specific DNA-binding protein based on the Lac repressor protein can isolate pre-purified DNA efficiently from simple buffer solution but our attempts to purify plasmids directly from crude starting materials were disappointing with impractically low DNA yields. We have optimize...

journal_title：Biotechnology and bioengineering

authors： Darby RA,Forde GM,Slater NK,Hine AV

更新日期：2007-12-01 00:00:00

abstract：:With a view toward reduction of graft loss, we explored pancreatic islet transplantation within fibrin matrices rendered pro-angiogenic by incorporation of minimal doses of vascular endothelial growth factor-A165 and platelet-derived growth factor-BB presented complexed to a fibrin-bound integrin-binding fibronectin d...

journal_title：Biotechnology and bioengineering

authors： Najjar M,Manzoli V,Abreu M,Villa C,Martino MM,Molano RD,Torrente Y,Pileggi A,Inverardi L,Ricordi C,Hubbell JA,Tomei AA

更新日期：2015-09-01 00:00:00

abstract：:Temperature shifting was investigated as a means of improving cloned-gene product yields form a recombinant Escherichia coli containing the temperature-sensitive plasmid, pOU 140. In a series of shaker flask fermentations recombinant cells were thermally induced for different time periods. The growth, stability, and p...

journal_title：Biotechnology and bioengineering

authors： Dipasquantonio VM,Betenbaugh MJ,Dhurjati P

更新日期：1987-03-01 00:00:00

abstract：:Esters were prepared by acylation of hydroxypropyl cellulose with fatty acid catalyzed by immobilized lipase from Candida antarctica in tert-butanol. The nature of the substrates used, the initial water activity of the system, and the molecular weight of the hydroxypropyl cellulose were investigated. Moreover, Fourier...

journal_title：Biotechnology and bioengineering

authors： Sereti V,Stamatis H,Pappas C,Polissiou M,Kolisis F

更新日期：2001-02-20 00:00:00

abstract：:The unicellular green alga Haematococcus pluvialis has been exploited as a cell factory to produce the high-value antioxidant astaxanthin for over two decades, due to its superior ability to synthesize astaxanthin under adverse culture conditions. However, slow vegetative growth under favorable culture conditions and ...

journal_title：Biotechnology and bioengineering

authors： Zhang Z,Wang B,Hu Q,Sommerfeld M,Li Y,Han D

更新日期：2016-10-01 00:00:00

abstract：:In the process of producing ethanol from lignocellulosic materials such as wheat straw, compounds that can act inhibitory to enzymatic hydrolysis and to cellular growth may be generated during the pretreatment. Ethanol production was evaluated on pretreated wheat straw hydrolysate using four different recombinant Sacc...

journal_title：Biotechnology and bioengineering

authors： Panagiotou G,Olsson L

更新日期：2007-02-01 00:00:00

abstract：:The objective of this study was to characterize the dynamic adaptation of the oxidative capacity of Saccharomyces cerevisiae to an increase in the glucose supply rate and its implications for the control of a continuous culture designed to produce biomass without allowing glucose to be diverted into the reductive meta...

journal_title：Biotechnology and bioengineering

authors： Duboc P,Cascão-Pereira LG,von Stockar U

更新日期：1998-03-05 00:00:00

abstract：:The amount of ATP produced during n-alkane, fatty acid, or acetate metabolism in Candida tropicalis has been established from the P/O ratios measured on isolated mitochondria, yield on substrate and carbon balance. For these three kinds of substrates YATP value has been found to be close to 4, although Ysub on acetate...

journal_title：Biotechnology and bioengineering

authors： Gallo M,Azoulay E

更新日期：1975-12-01 00:00:00

abstract：:Stem cell self-renewal versus differentiation fate decisions are difficult to characterize and analyze due to multiple competing rate processes occurring simultaneously among heterogeneous cell subpopulations. To address this challenge, we describe a mathematical model for cell population dynamics that allows flow cyt...

journal_title：Biotechnology and bioengineering

authors： Prudhomme WA,Duggar KH,Lauffenburger DA

更新日期：2004-11-05 00:00:00

abstract：:The simultaneous growth and product formation in a microbial culture is an important feature of several laboratory, industrial, and environmental bioprocesses. Metabolic burden associated with product formation in these bioprocesses may lead to growth advantage of a nonproducing mutant leading to a loss of the produci...

journal_title：Biotechnology and bioengineering

authors： Varma A,Palsson BO

更新日期：1994-02-20 00:00:00

abstract：:Chromatography is the method of choice for the separation of proteins, at both analytical and preparative scale. Orthogonal purification strategies for industrial use can easily be implemented by combining different modes of adsorption. Nevertheless, with flexibility comes the freedom of choice and optimal conditions ...

journal_title：Biotechnology and bioengineering

authors： Dismer F,Hansen S,Oelmeier SA,Hubbuch J

更新日期：2013-03-01 00:00:00

abstract：:This study demonstrates that masking the Feb regions of a monoclonal antibody (Mab) with synthetic antigens prior to covalent immobilization efficiency. Water-soluble adducts of poly(2-methyloxazoline) polymers and a synthetic-peptide epitope for the Mab were constructed. These synthetic antigens are referred to as Fa...

journal_title：Biotechnology and bioengineering

authors： Velander WH,Subramanian A,Madurawe RD,Orthner CL

更新日期：1992-04-25 00:00:00

abstract：:Lipases are versatile biocatalists showing multiple applications in a wide range of biotechnological processes. The gene lipA coding for Lipase A from Bacillus subtilis was isolated by PCR amplification, cloned and expressed in Escherichia coli, Saccharomyces cerevisiae and Bacillus subtilis strains, using pBR322, YEp...

journal_title：Biotechnology and bioengineering

authors： Sánchez M,Prim N,Rández-Gil F,Pastor FI,Diaz P

更新日期：2002-05-05 00:00:00

abstract：:High methanol concentrations have a negative effect on the growth rate and the biomass yield of growth transients induced by methanol pulses in continuous cultures of Methylomonas L3. The physiological basis of this effect is investigated by measuring the effect of the methanol pulse on the cell energy charge (EC) and...

journal_title：Biotechnology and bioengineering

authors： Chu IM,Papoutsakis ET

更新日期：1987-01-01 00:00:00

abstract：:A novel process has been developed which uses reversed micelles to isolate denatured protein molecules from each other and allows them to refold individually. These reversed micelles are aqueous phase droplets stabilized by the surfactant AOT and suspended in isooctane. By adjusting conditions such that only one prote...

journal_title：Biotechnology and bioengineering

authors： Hagen AJ,Hatton TA,Wang DI

更新日期：1990-04-25 00:00:00

abstract：:A novel technique for immobilization of Candida rugosa lipase onto anionic colloidal gas aphrons (CGAs) is described. CGAs are spherical microbubbles (10-100 microm) composed of an inner gas core surrounded by a surfactant shell. In this initial study, greater than 80% lipase (w/w) was effectively retained on the CGAs...

journal_title：Biotechnology and bioengineering

authors： O'Connell PJ,Varley J

更新日期：2001-08-05 00:00:00