Abstract:
:Temperature shifting was investigated as a means of improving cloned-gene product yields form a recombinant Escherichia coli containing the temperature-sensitive plasmid, pOU 140. In a series of shaker flask fermentations recombinant cells were thermally induced for different time periods. The growth, stability, and plasmid product levels were followed, and the results indicate the existence of an induction time period that maximizes product yield. A sustained thermal induction results in recombinant cell death and instability, while exposure to a runaway temperature for minimal time periods does not give sufficiently high product yields. At intermediate cycling times, however, the recombinant cells remain stable, and the plasmid replication region is activated, resulting in higher product yields.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Dipasquantonio VM,Betenbaugh MJ,Dhurjati Pdoi
10.1002/bit.260290416subject
Has Abstractpub_date
1987-03-01 00:00:00pages
513-9issue
4eissn
0006-3592issn
1097-0290journal_volume
29pub_type
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