Abstract:
:The kinetics of precipitation of soya protein has been examined in a tubular flow reactor with the precipitants, ammonium sulfate, ethanol, divalent calcium, and sulfuric acid. The precipitate growth profiles obtained in all cases showed the rapid formation of a precipitate phase and the attainment of a final size within 16 s. The final mean particle diameter d(m) varied with precipitating agent used in the order: sulfuric acid (12.5 mum) > ethanol (7.5 mum) approximately calcium ion (7.2 mum) > ammonium sulfate (3.1 mum). In the case of ethanol precipitation, changes in the design of the contacting section of the reactor led to differences in the precipitate growth curve. Protein solubility curves are also presented, and with the reactor data, they provide a convenient method for assessing the effect of precipitating agent on the design of protein precipitation reactors.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Chan MY,Hoare M,Dunnill Pdoi
10.1002/bit.260280312subject
Has Abstractpub_date
1986-03-01 00:00:00pages
387-93issue
3eissn
0006-3592issn
1097-0290journal_volume
28pub_type
杂志文章abstract::For in vitro liver replacement devices, such as packed bed bioreactors, to maintain the essential functions of the liver, they must at least successfully support hepatocytes, the parenchymal cell of the liver. In vivo, the liver is a major consumer of oxygen. Hence it is unsurprising that the limited transport distanc...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21681
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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更新日期:1992-06-05 00:00:00
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journal_title:Biotechnology and bioengineering
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