Abstract:
:In a stirred culture of hybridoma cells, the effects of serum reduction from 2.5% to 0% on growth and monoclonal antibody production have been investigated. The shear sensitivity of cells from the same culture has been tested in a bubble column. Serum reduction does not greatly affect viable-cell concentrations, but cell specific monoclonal-antibody production rate shows a decreasing trend. A gradual increase in sensitivity for sparging, which is nor the result of a long-term biological effect, has been measured in a bubble column at decreasing fetal calf serum concentrations. Finally, the hypothetical killing-volume model describing the death rate of insect cells in bubble columns has now been completely validated for the pertinent hybridoma-cell line.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
van der Pol L,Bakker WA,Tramper Jdoi
10.1002/bit.260400125subject
Has Abstractpub_date
1992-06-05 00:00:00pages
179-82issue
1eissn
0006-3592issn
1097-0290journal_volume
40pub_type
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