Abstract:
:Oil residues arising from the Christos-Bitas spillage were found to contain 28% of oil extractable by carbon tetrachloride; the remainder consisted of water and undefined solids. Christos-Bitas mousse was added to 1.18 m(3) liquor inoculated with oil-contaminated marine mud, and aerated with a 1.5-hp vortex pump and venturi nozzle (12.5 mm) in a cylindrical tank. After 70 days, oil degradation reached 7 mg oil/L/h. About 98% of the solvent extractable oil added was degraded over 83 days. Analysis of oil residues harvested at the end of this experiment showed that there was a decreasing trend in percent degradation in the following order: aromatics > saturates > heterocyclics > asphalts. No less than 94% of any fraction analysed was degraded.In the second pilot trial, oil degradation was carried out in a cylindrical jacket tank containing 6.82 m(3) liquor inoculated with oil-contaminated marine mud from Penarth, South Wales, UK, together with pure cultures derived from the same source, and aerated with a 7.5-hp vortex pump and venturi nozzle (18 mm diameter). Mixing of the oil was inhomogeneous for the first 100-110 days. The overall degree of substrate dispersion and total oil balance was determined by sampling at different depths. Degradation by the mixed culture was achieved at the rate of 164 mg oil/L/h. After 224 days, this was equivalent to 9.6 x 10(3)/kg(-1)/yr;(214 kg/wk) for 6.82 m(3) of liquor. The degradation rate continued to rise as the feed rate was increased by means of an automatic, timed pump. A lag phase of five to six months was necessary to allow the mixed population to build up to an exploitable level.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Berwick PGdoi
10.1002/bit.260270112subject
Has Abstractpub_date
1985-01-01 00:00:00pages
94-108issue
1eissn
0006-3592issn
1097-0290journal_volume
27pub_type
杂志文章abstract::This study describes the development of a transient expression system for CHO cells based on autonomous replication and retention of transfected plasmid DNA. A transient expression system that allows extrachromosomal amplification of plasmids permits more plasmid copies to persist in the transfected cell throughout th...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20534
更新日期:2005-09-20 00:00:00
abstract::Dielectric spectroscopy was applied to two industrial high cell density culture processes and used to determine on-line the concentration of CHO cells immobilized on macroporous microcarriers in a stirred bioreactor and in a packed-bed of disk carriers. The cell concentration predicted from the spectroscopic data was ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1197
更新日期:2002-02-05 00:00:00
abstract::We report the isolation of mutant strains of the methylotrophic yeast Hansenula polymorpha that are able to efficiently oxidize ethanol to acetaldehyde in an intact cell system. The oxidation reaction is catalyzed by alcohol oxidase (AOX), a key enzyme in the methanol metabolic pathway that is typically present only i...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(20000405)68:1<44::aid-bit5
更新日期:2000-04-05 00:00:00
abstract::A chimeric Fab was expressed in Chinese hamster ovary cells under the control of the CMV promoter in a two-stage production process. Cells were first grown to 90% confluence at 37 degrees C in a proliferation phase, followed by a production phase at either 37 degrees C or 28 degrees C. Medium supplemented with serum a...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10793
更新日期:2003-11-20 00:00:00
abstract::The possibility of enhancing the biomass productivity of a continuous culture of Saccharomyces cerevisiae growing on a glucose-limited medium is addressed. An unstructured Monod-type model is first identified using steady-state data. The culture is subjected to step changes in dilution rate, and it is seen that the Mo...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260340603
更新日期:1989-09-01 00:00:00
abstract::L-Threonine, a kind of essential amino acid, has numerous applications in food, pharmaceutical, and aquaculture industries. Fermentative l-threonine production from glucose has been achieved in Escherichia coli. However, there are still several limiting factors hindering further improvement of l-threonine productivity...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26844
更新日期:2019-01-01 00:00:00
abstract::Although one of the major factors limiting the application of Bacillus subtilis as an expression host has been its production of at least eight extracellular proteases, researchers have also noticed that some proteases benefited the secretion of foreign proteins at times. Therefore, to maximize the yield of a foreign ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26992
更新日期:2019-08-01 00:00:00
abstract::This communication demonstrates that two-phase aqueous mixed (nonionic/ionic) micellar systems have the potential for improving the separation of proteins from viruses. Specifically, two separation experiments were performed to show that the addition of the anionic surfactant sodium dodecyl sulfate (SDS) to the two-ph...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10377
更新日期:2002-10-20 00:00:00
abstract::James (Jay) E. Bailey was a pioneer in biotechnology and biochemical engineering. During his 30 years in academia he made seminal contributions to many fields of chemical engineering science, including catalysis and reaction engineering, bioprocess engineering, mathematical modeling of cellular processes, recombinant ...
journal_title:Biotechnology and bioengineering
pub_type: 传,历史文章,杂志文章
doi:10.1002/bit.10404
更新日期:2002-09-05 00:00:00
abstract::C-terminal lysine (C-K) variants are commonly observed in therapeutic monoclonal antibodies and recombinant proteins. Heterogeneity of C-K residues is believed to result from varying degree of proteolysis by endogenous carboxypeptidase(s) during cell culture production. The achievement of batch-to-batch culture perfor...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24510
更新日期:2012-09-01 00:00:00
abstract::Highly efficient antibody immobilization is extremely crucial for the development of high-performance polymeric microdevices for enzyme-linked immunosorbent assay (ELISA). In this article, a site-selective tyrosinase (TR)-catalyzed protein A strategy for antibody immobilization was developed to enhance the sensitivity...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22136
更新日期:2009-02-15 00:00:00
abstract::Fibrous proteins, such as silk, elastin and collagen are finding broad impact in biomaterial systems for a range of biomedical and industrial applications. Some of the key advantages of biosynthetic fibrous proteins compared to synthetic polymers include the tailorability of sequence, protein size, degradation pattern...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章,评审
doi:10.1002/bit.25820
更新日期:2016-05-01 00:00:00
abstract::Yeasts capable of fermenting both D-xylose and cellobiose to ethanol were screened. Of 213 species of yeasts surveyed, Kluyveromyces cellobiovorus sp. nov., a new species belonging to genus of Kluyveromyces, was selected as the sole strain. This strain accumulated 32, 22, and 19 g/L of ethanol from 8% glucose, D-xylos...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270417
更新日期:1985-04-01 00:00:00
abstract::Bioprocesses operated in batch mode can induce adverse effects like overflow metabolism, substrate inhibition, osmotic inhibition, oxygen limitation, and catabolite repression. To avoid these adverse effects, fed-batch is the predominant operation mode in industrial production. Nevertheless, screening for optimal prod...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27077
更新日期:2019-09-01 00:00:00
abstract::Electron paramagnetic resonance spectroscopy is used to observe hydroxyl radicals produced by an atmospheric pressure nonthermal plasma device at distances greater than 1 m from the discharge. The plasma device is an indirect treatment setup with closed loop airflow and hydrogen peroxide additives that is effective in...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24853
更新日期:2013-07-01 00:00:00
abstract::Isothermal microcalorimeters (IMC) are highly sensitive instruments that allow the measurement of heat flow in the microwatt range. Due to their detection of minute thermal heat, IMC techniques have been used in numerous biological applications, including the study of fermentation processes, pharmaceutical development...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23268
更新日期:2011-12-01 00:00:00
abstract::The study reports on the development of a bioreactor for the production of alpha-keto acids from D,L- or D-amino acids using Rhodotorula gracilis D-amino acid oxidase. D-amino acid oxidase was co-immobilized with catalase on Affi-Gel 10 matrix, and the reactor was operated as a continuous-stirred tank reactor (CSTR) o...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260441104
更新日期:1994-12-01 00:00:00
abstract::Foam separation may have potential for protein recovery. However, for foam separation to be a viable protein recovery technique it is important to demonstrate, not only that high enrichments and recoveries can be achieved for single proteins, but also that high enrichments and recoveries, together with selectivity of ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990205)62:3<291::aid-bit
更新日期:1999-02-05 00:00:00
abstract::The quality of cassava starch, an important trait in cassava breeding programs, determines its applications in various industries. For example, development of waxy (having a low level of amylose) cassava is in demand. Amylose is synthesized by granule-bound starch synthase I (GBSSI) in plants, and therefore, down-regu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23120
更新日期:2011-08-01 00:00:00
abstract::Somatic embryogenesis is among the most promising means of large scale plant micropropagation. The development of somatic embryos is characterized by their morphological changes. Embryos in culture usually exhibit high heterogeneity and abnormality. As conventional microscopic observation is laborious and subjective, ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960405)50:1<65::AID-BIT8
更新日期:1996-04-05 00:00:00
abstract::Centrifugal adsorption technology (CAT) is a new compact, countercurrent technology for efficient adsorption from large liquid streams by using adsorbent particles in the micrometer range. CAT seems particularly suited for the recovery of macromolecules at low concentrations, because the small particle dimensions lead...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10121
更新日期:2002-05-05 00:00:00
abstract::A computer-aided methodology is developed for on-line monitoring and control of cell growth in fed-batch penicillin fermentation using a semidefined medium containing low corn steep liquor concentration (5.7 g/L). Cell growth is monitored and controlled with the use of experimental correlation and carbon-balancing equ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260250118
更新日期:1983-01-01 00:00:00
abstract::Being biosurfactants, rhamnolipids create severe foaming when produced in aerobic Pseudomonas aeruginosa fermentation. The necessary reduction of aeration causes oxygen limitation and restricts cell and product concentrations. In this study, we evaluate the new strategy of rhamnolipid production under denitrification ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/1097-0290(20010105)72:1<25::aid-bit4>3.0.c
更新日期:2001-01-05 00:00:00
abstract::The high hydrophobicity of polycyclic aromatic hydrocarbons (PAHs) strongly reduces their bioavailability in aged contaminated soils, thus limiting their bioremediation. The biodegradation of PAHs in soils can be enhanced by employing surface-active agents. However, chemical surfactants are often recalcitrant and exer...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20225
更新日期:2004-10-20 00:00:00
abstract::The Ff filamentous bacteriophages show potential as a new class of therapeutics, displaying utility in materials science as well as pharmaceutical applications. These phages are produced by the infection of E. coli, a Gram-negative bacterium which unavoidably sheds endotoxins into the extracellular space during growth...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25571
更新日期:2015-08-01 00:00:00
abstract::Geotrichum candidum was cultivated at the surface of solid model media containing peptone to simulate the composition of Camembert cheese. The surface growth of G. candidum induced the diffusion of substrates from the core to the rind and the diffusion of produced metabolites from the rind to the core. In the range of...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20100
更新日期:2004-07-05 00:00:00
abstract::Most biotherapeutic drugs are recombinant monoclonal antibodies which are mostly produced in monoclonal cell lines derived from Chinese hamster ovary (CHO) cells. Various clones expressing a monoclonal recombinant antibody were analyzed and a correlation of the antibody concentration and the relative mRNA level of cal...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24527
更新日期:2012-10-01 00:00:00
abstract::Co-Immobilization of dextransucrase (DS) and dextranase (DN) into calcium alginate includes the co-entrapment of soluble DS and adsorbed DN. DS converts sucrose into dextran, which is the substrate for DN, so that isomalto-oligosaccharides (IMOs) are follow-up products of dextran hydrolysis. The boundary conditions fo...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21810
更新日期:2008-07-01 00:00:00
abstract::The development of new gene delivery technologies is a prerequisite towards gene therapy clinical trials. Because gene delivery mediated by viral vectors remains of limited scope due to immunological and propagation risks, the development of new non-viral gene delivery systems is of crucial importance. We have synthes...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::Ultrafiltration through Carbosep M(4) mineral membrane of protein solutions of decreasing complexity (whey before and after centrifugation or clarification, beta-lactoglobulin) was studied. Mathematical models were used to explain variations in flux with time. Taking into account variations in protein retention and hy...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260340205
更新日期:1989-06-20 00:00:00