A multi-site metastasis-on-a-chip microphysiological system for assessing metastatic preference of cancer cells.

abstract：:Olive oil hydrolysis using Chromobacterium viscosum lipase B in a reversed micellar media was investigated in a membrane reactor. The dynamic evolution of the product concentration both in the concentrate and permeate stream was analyzed using a mechanistic model previously developed by us and further modified in this...

journal_title：Biotechnology and bioengineering

authors： Prazeres DM,Lemos F,Garcia FA,Cabral JM

更新日期：1993-09-05 00:00:00

abstract：:As the therapeutic monoclonal antibody (mAb) market continues to grow, optimizing production processes is becoming more critical in improving efficiencies and reducing cost-of-goods in large-scale production. With the recent trends of increasing cell culture titers from upstream process improvements, downstream capaci...

journal_title：Biotechnology and bioengineering

authors： Dizon-Maspat J,Bourret J,D'Agostini A,Li F

更新日期：2012-04-01 00:00:00

abstract：:Modern large-scale agricultural practices that incorporate high density farming with subtherapeutic antibiotic dosing are considered a major contributor to the rise of antibiotic-resistant bacterial infections of humans with species of Salmonella being a leading agriculture-based bacterial infection. Microcin J25, a p...

journal_title：Biotechnology and bioengineering

authors： Ritter SC,Yang ML,Kaznessis YN,Hackel BJ

更新日期：2018-10-01 00:00:00

abstract：:The rate of diffusion of serum albumin (MW 6.9 x 10(4) D) out of beads of calcium alginate gels depends upon the concentration and uronic acid composition of the alginate (ManA/GulA ratio), the conditions under which the beads are produced, the pH, and the temperature. The diffusion coefficient decreases with increasi...

journal_title：Biotechnology and bioengineering

authors： Martinsen A,Storrø I,Skjårk-Braek G

更新日期：1992-01-20 00:00:00

abstract：:The possibility of biologically detoxifying a contaminated soil from an Italian dump site containing about 1500 mg/kg (in dry soil) of polychlorinated biphenyls was studied in the laboratory in this work. The soil, which contained indigenous aerobic bacteria capable of growing on biphenyl or on monochlorobenzoic acids...

journal_title：Biotechnology and bioengineering

authors： Fava F,Bertin L

更新日期：1999-07-20 00:00:00

abstract：:Growth decoupling can be used to optimize the production of biochemicals and proteins in cell factories. Inhibition of excess biomass formation allows for carbon to be utilized efficiently for product formation instead of growth, resulting in increased product yields and titers. Here, we used CRISPR interference to in...

journal_title：Biotechnology and bioengineering

authors： Landberg J,Wright NR,Wulff T,Herrgård MJ,Nielsen AT

更新日期：2020-08-18 00:00:00

abstract：:Despite wide applications of l-tyrosine in the market, microbial overproduction of l-tyrosine has been a great challenge due to the complex gene regulations involved in its biosynthetic pathway. To this end, effects of knocking out tyrR on the l-tyrosine production were further explored during the strain development. ...

journal_title：Biotechnology and bioengineering

authors： Kim B,Binkley R,Kim HU,Lee SY

更新日期：2018-10-01 00:00:00

abstract：:The effects of external calcium concentrations on biosynthesis of ginsenoside Rb1 and several calcium signal sensors were quantitatively investigated in suspension cultures of Panax notoginseng cells. It was observed that the synthesis of intracellular ginsenoside Rb1 in 3-day incubation was dependent on the medium Ca...

journal_title：Biotechnology and bioengineering

authors： Yue CJ,Zhong JJ

更新日期：2005-02-20 00:00:00

abstract：:Lipases are versatile biocatalists showing multiple applications in a wide range of biotechnological processes. The gene lipA coding for Lipase A from Bacillus subtilis was isolated by PCR amplification, cloned and expressed in Escherichia coli, Saccharomyces cerevisiae and Bacillus subtilis strains, using pBR322, YEp...

journal_title：Biotechnology and bioengineering

authors： Sánchez M,Prim N,Rández-Gil F,Pastor FI,Diaz P

更新日期：2002-05-05 00:00:00

abstract：:Cell-division-cycle, temperature-sensitive mutants of Saccharomyces cerevisiae were investigated as a means of altering the morphological characteristics and subsequent physical properties of single-cell protein (SCP). Strain 4471, harboring mutation cdc 4, formed a visible complex mass at the nonpermissive temperatur...

journal_title：Biotechnology and bioengineering

authors： Miyasaka Y,Rha C,Sinskey AJ

更新日期：1980-10-01 00:00:00

abstract：:Cultures with immobilized hybridoma cells were performed in fixed bed systems. "Steady state" values for volume-specific substrate uptake and metabolite production rates were determined at various perfusion rates and superficial flow velocities of the medium within the carrier matrix. Data from fixed bed volumes betwe...

journal_title：Biotechnology and bioengineering

authors： Pörtner R,Rössing S,Koop M,Lüdemann I

更新日期：1997-08-05 00:00:00

abstract：:Recombinant Escherichia coli harboring the medium-chain-length (MCL) polyhydroxyalkanoate (PHA) synthase gene has been shown to accumulate MCL-PHAs from fatty acids when FadB is inactive. However, the enzymes in fadB mutant E. coli responsible for channeling the beta-oxidation intermediates to PHA biosynthesis have no...

journal_title：Biotechnology and bioengineering

authors： Park SJ,Yup Lee S

更新日期：2004-06-20 00:00:00

abstract：:Intracellular events that take place during influenza virus replication in animal cells are well understood qualitatively. However, to better understand the complex interaction of the virus with its host cell and to quantitatively analyze the use of cellular resources for virion formation or the overall dynamic for th...

journal_title：Biotechnology and bioengineering

authors： Sidorenko Y,Reichl U

更新日期：2004-10-05 00:00:00

abstract：:A novel chemistry has been developed for the production of capsules composed of a hydrophobic liquid core surrounded by a cross-linked polyacrylamide/alginate membrane. These liquid-core capsules may be used in capsular perstraction for the removal of inhibitory products from bioprocesses and bioconversions. They have...

journal_title：Biotechnology and bioengineering

authors： Wyss A,von Stockar U,Marison IW

更新日期：2004-06-05 00:00:00

abstract：:Generating stable, high-producing cell lines for recombinant protein production requires an understanding of the potential limitations in the cellular machinery for protein expression. In order to increase our understanding of what makes a stable high producer, we have generated a panel of 17 recombinant monoclonal an...

journal_title：Biotechnology and bioengineering

authors： Chusainow J,Yang YS,Yeo JH,Toh PC,Asvadi P,Wong NS,Yap MG

更新日期：2009-03-01 00:00:00

abstract：:Refiner mechanical pulp was biologically treated with several higher fungi in order to test their potential for increasing the strength of paper. It was among the white-rot fungi that the best results were obtained. Polyporus versicolor gave the best overall improvement in handsheet properties with no reduction in tea...

journal_title：Biotechnology and bioengineering

authors： Pilon L,Barbe MC,Desrochers M,Jurasek L,Neumann PJ

更新日期：1982-09-01 00:00:00

abstract：:The twin arginine translocation (Tat) pathway occurs naturally in E. coli and has the distinct ability to translocate folded proteins across the inner membrane of the cell. It has the potential to export commercially useful proteins that cannot be exported by the ubiquitous Sec pathway. To better understand the biopro...

journal_title：Biotechnology and bioengineering

authors： Branston SD,Matos CF,Freedman RB,Robinson C,Keshavarz-Moore E

更新日期：2012-04-01 00:00:00

abstract：:A model that portrays substrate profiles in a steady-state multispecies granular biofilm is developed and coupled with a biofilm detachment model. The model accounts for glucose, propionate, hydrogen, and acetate transformations performed by three bacterial trophic groups: acidogens, syntrophic bacterial consortia, an...

journal_title：Biotechnology and bioengineering

authors： Tartakovsky B,Guiot SR

更新日期：1997-04-20 00:00:00

abstract：:Bacterial biofilms are a major obstacle challenging the development of more effective therapies to treat implant infections. Oxygen availability to bacterial cells has been implicated in biofilm formation and planktonic cell detachment; however, there are insufficient tools available to measure oxygen concentrations w...

journal_title：Biotechnology and bioengineering

authors： Acosta MA,Velasquez M,Williams K,Ross JM,Leach JB

更新日期：2012-10-01 00:00:00

abstract：:Current human gene therapy relies on genetic modification of the patient's own cells. An alternate nonautologous approach is to use universal cell lines engineered to secrete therapeutic products. Protection with immunoisolation devices before implantation would allow the use of the same recombinant cell line for trea...

journal_title：Biotechnology and bioengineering

authors： Tse M,Uludag H,Sefton MV,Chang PL

更新日期：1996-08-05 00:00:00

abstract：:Bovine pancreatic trypsin was treated with ethylene glycol bis(succinic acid N-hydroxysuccinimide ester). Approximately 8 of 14 lysines per trypsin molecule were modified. This derivative (EG trypsin) was more stable than native between 30 degrees and 70 degrees C: T50 values were 59 degrees C and 46 degrees C, respec...

journal_title：Biotechnology and bioengineering

authors： Murphy A,O Fágáin C

更新日期：1998-05-20 00:00:00

abstract：:Pronounced spatial nonuniformities in cell density, physiology, and activity frequently arise within densely packed immobilized cell supports. For a more fundamental understanding of immobilized cell phenomena, we have developed high-resolution microfluorimetric procedures to analyze local variations in both immobiliz...

journal_title：Biotechnology and bioengineering

authors： Kuhn RH,Peretti SW,Ollis DF

更新日期：1991-08-05 00:00:00

abstract：:The in vitro generation of cell-based three dimensional (3D) nerve tissue is an attractive subject to improve graft survival and integration into host tissue for neural tissue regeneration or to model biological events in stem cell differentiation. Although 3D organotypic culture strategies are well established for 3D...

journal_title：Biotechnology and bioengineering

authors： Kim BC,Jun SM,Kim SY,Kwon YD,Choe SC,Kim EC,Lee JH,Kim J,Suh JF,Hwang YS

更新日期：2017-04-01 00:00:00

abstract：:The compound 5-hydroxymethylfurfural (HMF) has attracted much attention due to its versatility as an important bio-based platform chemical. Here, we engineered Raoultella ornithinolytica BF60 as a whole-cell biocatalyst for a highly efficient synthesis of 2,5-furandicarboxylic acid (FDCA) from HMF. Specifically, vario...

journal_title：Biotechnology and bioengineering

authors： Yuan H,Liu Y,Li J,Shin HD,Du G,Shi Z,Chen J,Liu L

更新日期：2018-09-01 00:00:00

abstract：:There has been a recent drive in commercial large-scale production of biotechnology products to convert current batch mode processing to continuous processing manufacturing. There have been reports of model systems capable of adapting and linking upstream and downstream technologies into a continuous manufacturing pip...

journal_title：Biotechnology and bioengineering

authors： Johnson SA,Brown MR,Lute SC,Brorson KA

更新日期：2017-01-01 00:00:00

abstract：:Pretreatment plays an important role in the efficient enzymatic hydrolysis of biomass into fermentable sugars for biofuels. A highly effective pretreatment method is reported for corn stover which combines mild alkali-extraction followed by ionic liquid (IL) dissolution of the polysaccharides and regeneration (recover...

journal_title：Biotechnology and bioengineering

authors： Geng X,Henderson WA

更新日期：2012-01-01 00:00:00

abstract：:Screening fifteen strains of white-rot fungi for their ability to decolorize combined bleached kraft effluent showed that Coriolus versicolor in liquid culture removed over 60% of the color of the effluent within six days in the presence of sucrose. Treatment of the same effluent with this fungus, immobilized in beads...

journal_title：Biotechnology and bioengineering

authors： Livernoche D,Jurasek L,Desrochers M,Dorica J,Veliky IA

更新日期：1983-08-01 00:00:00

abstract：:To investigate the effects of factors secreted by different cell lines on human monoclonal antibody (MAb) integrity, 600 mg of a human MAb, which specifically binds to human erythrocytes, were produced in a perfusion process. After purification by protein A affinity chromatography, the MAb was used for integrity testi...

journal_title：Biotechnology and bioengineering

authors： Ackermann M,Jäger V,Marx U

更新日期：1995-01-20 00:00:00

abstract：:The study was done to evaluate the efficacy of using reuterin produced by Lactobacillus reuteri to sanitize biological tissues. The microorganism tested in the study was Pseudomonas aeruginosa, a common cause of nosocomial biomaterial-related infections. The inhibitory effect of reuterin on P. aeruginosa for an inocul...

journal_title：Biotechnology and bioengineering

authors： Liang HF,Chen CN,Chang Y,Sung HW

更新日期：2003-10-20 00:00:00

abstract：:The dissociation constant, K(d) , is an important parameter for characterizing protein-protein interaction affinities. SUMOylation is one of the important protein post-translational modifications and it involves a multi-step enzymatic cascade reaction, resulting in peptide activation and substrate conjugation. Multipl...

journal_title：Biotechnology and bioengineering

authors： Song Y,Rodgers VG,Schultz JS,Liao J

更新日期：2012-11-01 00:00:00