Use of exogenous specialised bacteria in the biological detoxification of a dump site-polychlorobiphenyl-contaminated soil in slurry phase conditions


:The possibility of biologically detoxifying a contaminated soil from an Italian dump site containing about 1500 mg/kg (in dry soil) of polychlorinated biphenyls was studied in the laboratory in this work. The soil, which contained indigenous aerobic bacteria capable of growing on biphenyl or on monochlorobenzoic acids at concentration of about 300 CFU per g of air-dried soil, was amended with inorganic nutrients, saturated with water and treated in aerobic 3-L batch slurry reactors (soil suspension at 20% w/v). Either Pseudomonas sp. CPE1 strain, capable of cometabolising low-chlorinated biphenyls into chlorobenzoic acids, or a bacterial co-culture capable of aerobically dechlorinating polychlorobiphenyls constituted by this bacterium and the two chlorobenzoic acid degrading bacteria Pseudomonas sp. CPE2 strain and Alcaligenes sp. CPE3 strain, were used as inocula (final concentration of about 10(8) CFU/mL for each bacterium), in the absence and in the presence of biphenyl (4 g/kg of air dried soil). Significant soil polychlorobiphenyl depletions were observed in all the reactors after 119 days of treatment. The soil inoculation with the sole CPE1 was found to slightly enhance the polychlorobiphenyl depletions (about 20%) and the soil detoxification; the effect was higher in the presence of biphenyl. The use of the polychlorobiphenyl mineralising bacterial co-culture as inoculum resulted in a strong enhancement of the depletions of both the soil polychlorobiphenyls (from 50 to 65%) and of the original soil ecotoxicity. The bacterial biomass inoculated was found to implant into the soil; the higher specialised biomass availability thus reached in the inoculated soil was probably responsible of a more extensive biodegradation of polychlorobiphenyls and therefore of the higher detoxification yields observed in the inoculated reactors. The soil ecotoxicity, measured through two different soil contact assays, i.e., the Lepidium sativum germination test and the Collembola mortality test, was often found to decrease proportionally with the soil polychlorobiphenyl concentration. Copyright 1999 John Wiley & Sons, Inc.


Biotechnol Bioeng


Fava F,Bertin L




Has Abstract


1999-07-20 00:00:00














  • Dependence of biological treatment rate on species composition in activated sludge or biofilm. II: From models to theory.

    abstract::Traditional biological treatment models are "deduced" from formal chemical kinetics or dynamics of pure microorganism cultures growth. The best formal models give reasonable approximations of the biological treatment model with an ecosystem adaptation (ESA model). The model presented here explains some features of the...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Vavilin VA

    更新日期:1983-06-01 00:00:00

  • Engineering the pentose phosphate pathway to improve hydrogen yield in recombinant Escherichia coli.

    abstract::Among various routes for the biological hydrogen production, the NAD(P)H-dependent pentose phosphate (PP) pathway is the most efficient for the dark fermentation. Few studies, however, have focused on the glucose-6-phosphate 1-dehydrogenase, encoded by zwf, as a key enzyme activating the PP pathway. Although the gluco...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kim YM,Cho HS,Jung GY,Park JM

    更新日期:2011-12-01 00:00:00

  • The effect of organic solvents on the equilibrium position of enzymatic acylglycerol synthesis.

    abstract::The effect of organic solvents on the equilibrium position of lipase-catalyzed esterification of glycerol and decanoic acid has been investigated. The reaction is carried out in an aqueous-organic two-phase system. In polar solvents, high mole fractions of monoacylglycerol and low mole fractions of triacylglycerol and...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Janseen AE,Van der Padt A,Van Sonsbeek HM,Van't Riet K

    更新日期:1993-01-05 00:00:00

  • The effect of medium composition on the acetone-butanol fermentation in continuous culture.

    abstract::The effects of growing Clostridium acetobutylicum NCIB 8052 in a chemostat under conditions of glucose, NH(4) (+), PO(4) (3-), Mg(2+), and Fe(2+) limitation were examined. It was noted that limitation of any major nutrient resulted in the same fermentation pattern. Conditions where minor nutrient levels were reduced a...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: McNeil B,Kristiansen B

    更新日期:1987-02-01 00:00:00

  • Covalent immobilization of redox enzyme on electrospun nonwoven poly(acrylonitrile-co-acrylic acid) nanofiber mesh filled with carbon nanotubes: a comprehensive study.

    abstract::In this work, novel conductive composite nanofiber mesh possessing reactive groups was electrospun from solutions containing poly(acrylonitrile-co-acrylic acid) (PANCAA) and multi-walled carbon nanotubes (MWCNTs) for redoxase immobilization, assuming that the incorporated MWCNTs could behave as electrons transferor du...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wang ZG,Ke BB,Xu ZK

    更新日期:2007-07-01 00:00:00

  • Characteristics and role of dynamic membrane layer in anaerobic membrane bioreactors.

    abstract::A submerged anaerobic dynamic membrane bioreactor (AnDMBR) was operated for treatment of concentrated wastewater. The dynamic membrane (DM) or cake layer was characterized on its physicochemical and biological composition and the role of the DM layer in treatment and filtration performances was assessed. The results s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ersahin ME,Tao Y,Ozgun H,Spanjers H,van Lier JB

    更新日期:2016-04-01 00:00:00

  • Individual and combined effects of the rolA, B, and C genes on anthraquinone production in Rubia cordifolia transformed calli.

    abstract::It is known that the rolA, rolB, and rolC genes of Agrobacterium rhizogenes T-DNA affect processes of plant development and activate the synthesis of secondary metabolites in transformed plant cells. Although a synergistic activity of the rol genes on root formation is well-documented, little is known about their indi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Shkryl YN,Veremeichik GN,Bulgakov VP,Tchernoded GK,Mischenko NP,Fedoreyev SA,Zhuravlev YN

    更新日期:2008-05-01 00:00:00

  • Synthesis of 3-tert-butylcatechol by an engineered monooxygenase.

    abstract::Recombinant Escherichia coli JM101 was used for the in vivo biocatalytic synthesis of 3-tert-butyl- catechol. The bacterial strain synthesized the laboratory-evolved variant HbpA(T2) of 2-hydroxybiphenyl 3-monooxygenase (HbpA, EC from Pseudomonas azelaica HBP1. The mutant enzyme HbpA(T2) is able to hydroxy...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Meyer A,Held M,Schmid A,Kohler HP,Witholt B

    更新日期:2003-03-05 00:00:00

  • Immunoaffinity removal of xenoreactive antibodies using modified dialysis or microfiltration membranes.

    abstract::Hyperacute rejection following xenogeneic transplantation in primates is mediated by naturally occurring IgM antibodies, which are specifically directed to alpha-Galactosyl residues on many nonprimate mammalian cells. Current approaches to remove these anti-alphaGal IgM include plasmapheresis followed by immunoaffinit...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Karoor S,Molina J,Buchmann CR,Colton C,Logan JS,Henderson LW

    更新日期:2003-01-20 00:00:00

  • Biodegradation of PCBs in two-phase partitioning bioreactors following solid extraction from soil.

    abstract::This article demonstrates the feasibility of a novel process concept for the remediation of PCB contaminated soil. The proposed process consists of PCB extraction from soil using solid polymer beads, followed by biodegradation of the extracted PCBs in a solid-liquid two-phase partitioning bioreactor (TPPB), where PCBs...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Rehmann L,Daugulis AJ

    更新日期:2008-04-01 00:00:00

  • Theoretical distribution of truncation lengths in incremental truncation libraries.

    abstract::Incremental truncation is a method for constructing libraries of every one base pair truncation of a segment of DNA. Incremental truncation libraries can be created using a time-dependent nuclease method or through the incorporation of alpha-phosphothioate dNTPs by PCR or by primer extension (THIO(pcr) truncation and ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ostermeier M

    更新日期:2003-06-05 00:00:00

  • Enhancement of ethanol and biogas production from high-crystalline cellulose by different modes of NMO pretreatment.

    abstract::Pretreatment of high-crystalline cellulose with N-methyl-morpholine-N-oxide (NMO or NMMO) to improve bioethanol and biogas production was investigated. The pretreatments were performed at 90 and 120 degrees C for 0.5-15 h in three different modes, including dissolution (85% NMO), ballooning (79% NMO), and swelling (73...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Jeihanipour A,Karimi K,Taherzadeh MJ

    更新日期:2010-02-15 00:00:00

  • Multivariable control of alcohol concentrations in the production of polyhydroxyalkanoates (PHAs) by Paracoccus denitrificans.

    abstract::A novel multivariable control strategy is developed for alcohol (ethanol and n-pentanol) concentrations in the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(HB-co-HV), a biodegradable polymer by Paracoccus denitrificans ATCC 1774. This controller, which is developed to control the mole fraction of P(HB...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chanprateep S,Abe N,Shimizu H,Yamane T,Shioya S

    更新日期:2001-07-20 00:00:00

  • The acetone butanol fermentation on glucose and xylose. I. Regulation and kinetics in batch cultures.

    abstract::The kinetics in batch culture of the acetone butanol fermentation by Clostridium acetobutylicum is compared on glucose, xylose, and mixtures of both sugars. The fastest initial growth and transition from an acid to a solvent metabolism occurs on glucose, with a final 62 g/L glucose conversion. On xylose, an initial sl...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Fond O,Engasser JM,Matta-El-Amouri G,Petitdemange H

    更新日期:1986-02-01 00:00:00

  • Flux balance analysis of CHO cells before and after a metabolic switch from lactate production to consumption.

    abstract::Mammalian cell cultures typically exhibit an energy inefficient phenotype characterized by the consumption of large quantities of glucose and the concomitant production of large quantities of lactate. Under certain conditions, mammalian cells can switch to a more energy efficient state during which lactate is consumed...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Martínez VS,Dietmair S,Quek LE,Hodson MP,Gray P,Nielsen LK

    更新日期:2013-02-01 00:00:00

  • Subphysiological temperature induces pervasive alternative splicing in Chinese hamster ovary cells.

    abstract::RNA sequencing (RNASeq) has been widely used to associate alterations in Chinese hamster ovary (CHO) cell gene expression with bioprocess phenotypes; however, alternative messenger RNA (mRNA) splicing, has thus far, received little attention. In this study, we utilized RNASeq for transcriptomic analysis of a monoclona...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Tzani I,Monger C,Motheramgari K,Gallagher C,Hagan R,Kelly P,Costello A,Meiller J,Floris P,Zhang L,Clynes M,Bones J,Barron N,Clarke C

    更新日期:2020-08-01 00:00:00

  • A comparative study of solid and liquid non-aqueous phases for the biodegradation of hexane in two-phase partitioning bioreactors.

    abstract::A comparative study of the performance of solid and liquid non-aqueous phases (NAPs) to enhance the mass transfer and biodegradation of hexane by Pseudomonas aeruginosa in two-phase partitioning bioreactors (TPPBs) was undertaken. A preliminary NAP screening was thus carried out among the most common solid and liquid ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Hernández M,Quijano G,Thalasso F,Daugulis AJ,Villaverde S,Muñoz R

    更新日期:2010-08-01 00:00:00

  • Utilization of light for the assimilation of organic matter in Chlorella sp. VJ79.

    abstract::Chlorella sp. strain VJ79 was isolated from a total heterotrophic count of a wastewater collector. It grows autotrophically, heterotrophically, and mixotrophically on a variety of organic substrates. Glucose and serine promote a mixotrophic growth from which the yield is higher than the sum of autotrophic and heterotr...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Lalucat J,Imperial J,Parés R

    更新日期:1984-07-01 00:00:00

  • Anaerobic methanethiol degradation in upflow anaerobic sludge bed reactors at high salinity (> or =0.5 M Na(+)).

    abstract::The feasibility of anaerobic methanethiol (MT) degradation at elevated sodium concentrations was investigated in a mesophilic (30 degrees C) lab-scale upflow anaerobic sludge bed (UASB) reactor, inoculated with estuarine sediment originating from the Wadden Sea (The Netherlands). MT was almost completely degraded (>95...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: van Leerdam RC,de Bok FA,Lens PN,Stams AJ,Janssen AJ

    更新日期:2007-09-01 00:00:00

  • Design and construction of improved new vectors for Zymomonas mobilis recombinants.

    abstract::Zymomonas mobilis is a very important gram-negative bacterium having a potential application to simultaneous co-production of biofuel and other high value-added products through biorefinery process technology development. Up to now, pLOI193 has been used as the plasmid of choice for Z. mobilis strains. However, its ap...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Dong HW,Bao J,Ryu DD,Zhong JJ

    更新日期:2011-07-01 00:00:00

  • A novel purification strategy for retrovirus gene therapy vectors using heparin affinity chromatography.

    abstract::Membrane separation and chromatographic technologies are regarded as an attractive alternative to conventional academic small-scale ultracentrifugation procedures used for retrovirus purification. However, despite the increasing demands for purified retroviral vector preparations, new chromatography adsorbents with hi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Segura MM,Kamen A,Trudel P,Garnier A

    更新日期:2005-05-20 00:00:00

  • Some comments on respiratory quotient (RQ) determination from the analysis of exit gas from a fermentor.

    abstract::Recently, the respiratory quotient (RQ) of microbes measured in situ in a fermentor by exit-gas analysis has been used successfully, for instance, in a fed-batch culture of baker's yeast as a criterion to control the feeding rate.(1-3) It is significant here to keep RQ values close to unity throughout; any deviations ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Aiba S,Furuse H

    更新日期:1990-08-20 00:00:00

  • Use of the abiotic proton balance for determining linear relations among net conversion rates of primary metabolites in fermentation processes.

    abstract::Linear relations among net conversion rates of primary metabolites in fermentation processes can be determined by using the macroscopic or pathway balancing methods. It is shown that the process observability can be improved by incorporating both the abiotic proton balance and the pH control reagent addition rate meas...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Siano SA

    更新日期:1996-12-20 00:00:00

  • Modeling lipolysis in a reversed micellar system: part II-membrane reactor.

    abstract::Olive oil hydrolysis using Chromobacterium viscosum lipase B in a reversed micellar media was investigated in a membrane reactor. The dynamic evolution of the product concentration both in the concentrate and permeate stream was analyzed using a mechanistic model previously developed by us and further modified in this...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Prazeres DM,Lemos F,Garcia FA,Cabral JM

    更新日期:1993-09-05 00:00:00

  • Recovery of insect cells using hollow fiber microfiltration.

    abstract::An efficient method was developed for media separation and cell collection for eukaryotic cells growing in suspension. The method is based on tangential flow microfiltration using an open channel arrangement in a hollow fiber configuration. Best results (highest processing flux rate) for polysulfone hollow fibers were...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Trinh L,Shiloach J

    更新日期:1995-11-20 00:00:00

  • Oil and eicosapentaenoic acid production by the diatom Phaeodactylum tricornutum cultivated outdoors in Green Wall Panel (GWP®) reactors.

    abstract::Phaeodactylum tricornutum is a widely studied diatom and has been proposed as a source of oil and polyunsaturated fatty acids (PUFA), particularly eicosapentaenoic acid (EPA). Recent studies indicate that lipid accumulation occurs under nutritional stress. Aim of this research was to determine how changes in nitrogen ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Rodolfi L,Biondi N,Guccione A,Bassi N,D'Ottavio M,Arganaraz G,Tredici MR

    更新日期:2017-10-01 00:00:00

  • Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection.

    abstract::Transfection with polyethylenimine (PEI) was evaluated as a method for the generation of recombinant Chinese hamster ovary (CHO DG44) cell lines by direct comparison with calcium phosphate-DNA coprecipitation (CaPO4) using both green fluorescent protein (GFP) and a monoclonal antibody as reporter proteins. Following t...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chenuet S,Martinet D,Besuchet-Schmutz N,Wicht M,Jaccard N,Bon AC,Derouazi M,Hacker DL,Beckmann JS,Wurm FM

    更新日期:2008-12-01 00:00:00

  • Monitoring of adherent live cells morphology using the undecimated wavelet transform multivariate image analysis (UWT-MIA).

    abstract::Cell morphology is an important macroscopic indicator of cellular physiology and is increasingly used as a mean of probing culture state in vitro. Phase contrast microscopy (PCM) is a valuable tool for observing live cells morphology over long periods of time with minimal culture artifact. Two general approaches are c...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Juneau PM,Garnier A,Duchesne C

    更新日期:2017-01-01 00:00:00

  • Insect larval expression process is optimized by generating fusions with green fluorescent protein.

    abstract::The insect larvae/baculovirus protein production process was dramatically simplified by expressing fusion proteins containing green fluorescent protein (GFP) and the product-of-interest. In this case, human interleukin-2 (hIL-2) and chloramphenicol acetyl-transferase (CAT) were model products. Specifically, our fusion...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Cha HJ,Dalal NG,Pham MQ,Vakharia VN,Rao G,Bentley WE

    更新日期:1999-11-05 00:00:00

  • Suggestions for the operation of radial flow cells in cell adhesion and biofouling studies.

    abstract::An analysis of the radial flow cell is presented to show that the assumption of creeping laminar flow should be used cautiously. Simple models which account for the influence of fluid inertial forces over most of the width of the plate are reviewed. A modified Reynolds number is introduced which may be used to test th...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Fryer PJ,Slater NK,Duddridge JE

    更新日期:1985-04-01 00:00:00