Abstract:
:Ralstonia eutropha grows on and produces polyhydroxyalkanoates (PHAs) from fermentation acids. Acetic acid, one major organic acid from acidogenesis of organic wastes, has an inhibitory effect on the bacterium at slightly alkaline pH (6 g HAc/L at pH 8). The tolerance of R. eutropha to acetate, however, was increased significantly up to 15 g/L at the slightly alkaline pH level with high cell mass concentration. A metabolic cell model with five fluxes is proposed to depict the detoxification mechanism including mass transfer and acetyl-CoA formation of acetic acid and the formation of three final metabolic products, polyhydroxybutyrate (PHB), active biomass, and CO(2). The fluxes were measured under different conditions such as cell mass concentration, acetic acid concentration, and medium composition. The experimental results indicate that the acetate detoxification by high cell mass concentration is attributed to the increased fluxes at high extracellular acetate concentrations. The fluxes could be doubled to reduce and hence detoxify the accumulated intracellular acetate anions.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Yu J,Wang Jdoi
10.1002/bit.1080subject
Has Abstractpub_date
2001-06-20 00:00:00pages
458-64issue
6eissn
0006-3592issn
1097-0290pii
10.1002/bit.1080journal_volume
73pub_type
杂志文章abstract::The in vitro generation of cell-based three dimensional (3D) nerve tissue is an attractive subject to improve graft survival and integration into host tissue for neural tissue regeneration or to model biological events in stem cell differentiation. Although 3D organotypic culture strategies are well established for 3D...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26205
更新日期:2017-04-01 00:00:00
abstract::The effector functions elicited by IgG antibodies strongly depend on the carbohydrate moiety linked to the Fc region of the protein. Therefore several approaches have been developed to rationally manipulate these glycans and improve the biological functions of the antibody. Overexpression of recombinant beta1,4-N-acet...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20777
更新日期:2006-04-05 00:00:00
abstract::The transient and steady-state responses of hybridoma growth and metabolism to glutamine pulse and step changes have been examined. Metabolic quotients are reported for oxygen, glucose, lactate, ammonia, glutamine, alanine, and other amino acids. The specific glutamine consumption rate increased rapidly after all glut...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260330414
更新日期:1989-01-20 00:00:00
abstract::Surface modified bioactive glass with surface properties akin to those of the bone mineral phase is an attractive candidate for use as a microcarrier material for 3-D growth of bone-like tissue in rotating wall vessel bioreactors (RWVs). The critical surface properties of this material are the result of reaction in so...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10023
更新日期:2001-11-05 00:00:00
abstract::Relatively conservative modifications of three proteins were carried out to alter their surface properties. The protein properties modified were hydrophobicity and charge. This was done by acylation of amino groups with anhydrides. For the hydrophobic modification experiments, two proteins (beta-lactoglobulin and bovi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960205)49:3<290::AID-BIT
更新日期:1996-02-05 00:00:00
abstract::Purified rat liver phenylalanine hydroxylase [L-phenylalanine:tetrahydropteridine:oxygen oxidoreductase (4-hydroxylating), EC 1.14.16.1] was immobilized with activated thiol-Sepharose 4B via disulfide bond formation, which is expected to immobilize the enzyme in its activated form through the SH modification. This imm...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280422
更新日期:1986-04-01 00:00:00
abstract::In this study we report the isolation of four denitrifying bacteria from a batch reactor, where the progress of hydrogenotrophic denitrification was examined. Only three of the strains had the ability to use hydrogen as electron donor. In the present work, kinetic batch experiments were carried out in order to study t...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21031
更新日期:2006-10-20 00:00:00
abstract::The production of dry-grind corn ethanol results in the generation of intermediate products, thin and whole stillage, which require energy-intensive downstream processing for conversion into commercial animal feed products. Hydrothermal carbonization of thin and whole stillage coupled with anaerobic digestion was inve...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24924
更新日期:2013-10-01 00:00:00
abstract::The kinetics of cellular reproduction and the rate and extent of synthesis of extracellular polymeric substances (EPS) were investigated for P. aeruginosa growing in glucose-limited chemostats. mu(max) and K(s) estimates of 0.4 h(-1) and 2 mg glucose C/L, respectively, at 25 degrees C were obtained for this bacterium....
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260261203
更新日期:1984-12-01 00:00:00
abstract::The conditions for the introduction of active carbonate groups into supports containing hydroxyl groups by reaction with 5-norbornene-2.3-dicarboximido carbonochloridate are described. Up to 1.5 mmol carbonate groups/g dry Sepharose 4B could be bound. In the case of glycine the reaction of the activated supports with ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260330105
更新日期:1989-01-05 00:00:00
abstract::A two-parameter deactivation model is proposed to describe the kinetics of activity stabilization for some enzymes. The single-step unimolecular mechanism exhibits non-first-order deactivation kinetics since the final enzyme state, E(1) is not completely inactivated. The usefulness of the model is demonstrated by appl...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260300604
更新日期:1987-10-20 00:00:00
abstract::Batch hydrolysis kinetics of paper birch (Betula papyrifera) xylan and its associated acetyl groups in dilute sulfuric acid have been measured for acid concentrations of between 0.04 and 0.18M and temperatures of between 100 and 170 degrees C. Only 5% of the cellulose was hydrolyzed for up to 85% xylan removal. Rate d...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270321
更新日期:1985-03-01 00:00:00
abstract::The production and specific productivity of hepatitis B surface antigen (HBsAg) in recombinant Chinese hamster ovary (CHO) cells were increased by 81% and threefold, respectively, when supplemented with 1.5% dimethyl sulfoxide (DMSO) in the culture medium. To investigate the mechanism of DMSO effect on HBsAg productio...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21262
更新日期:2007-06-15 00:00:00
abstract::In order to evaluate the sensitivity of hepatocellular cultures to variations in both substrate stiffness and bioactive ligand presentation, hepatocytes were cultured on differentially compliant polyacrylamide gel discs functionalized with varying amounts of the ECM ligand, fibronectin (FN). Subconfluent cell cultures...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20328
更新日期:2005-02-05 00:00:00
abstract::After periodate oxidation of its glycosidic component, invertase was covalently bound onto three types of modified solid supports: glycidyl methacrylate, styrene-divinylbenzene copolymers, and bead cellulose. Direct reaction of the invertase aldehyde groups that were formed with amino groups of the support and use of ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260261011
更新日期:1984-10-01 00:00:00
abstract::The flotation of Streptomyces pilosus was studied in the absence and presence of adsorbed Pb(II) ions on the cell walls and with and without using sodium dodecylsulfate (SDS) as collector. Adsorption of SDS onto the organism in the absence and presence of Pb(II) ions was also investigated. It was found that in the abs...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260371009
更新日期:1991-04-25 00:00:00
abstract::This article reports a study of the performance of membrane-attached biofilms grown in a single tube extractive membrane bioreactor (STEMS) used for the treatment of a synthetic wastewater containing a toxic VOC (1,2-dichloroethane [DCE]). Mass balances show that complete mineralization of DCE was achieved, and that t...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260470111
更新日期:1995-07-05 00:00:00
abstract::Recent studies have demonstrated the feasibility of using ultrafiltration for the purification of plasmid DNA, but there is still little understanding of the factors governing DNA transmission. Experimental data were obtained for the transmission of a 3.0 kbp supercoiled plasmid DNA through composite regenerated cellu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21575
更新日期:2008-02-01 00:00:00
abstract::Increased stability at alkaline pH should be a valuable attribute for the utilization of penicillin acylase in bioreactors employed to convert penicillins into 6-aminopenicillanic acid, a precursor of semisynthetic penicillins. In these systems, base is added for pH control, which results in local alkaline conditions ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260480208
更新日期:1995-10-20 00:00:00
abstract::The effect of formate and hydrogen on isomerization and syntrophic degradation of butyrate and isobutyrate was investigated using a defined methanogenic culture, consisting of syntrophic isobutyrate-butyrate degrader strain IB, Methanobacterium formicicum strain T1N, and Methanosarcina mazeii strain T18. Formate and h...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19961105)52:3<404::AID-BIT
更新日期:1996-11-05 00:00:00
abstract::Ion-exchange (IEX) chromatography steps are widely applied in protein purification processes because of their high capacity, selectivity, robust operation, and well-understood principles. Optimization of IEX steps typically involves resin screening and selection of the pH and counterion concentrations of the load, was...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21905
更新日期:2008-08-01 00:00:00
abstract::Being biosurfactants, rhamnolipids create severe foaming when produced in aerobic Pseudomonas aeruginosa fermentation. The necessary reduction of aeration causes oxygen limitation and restricts cell and product concentrations. In this study, we evaluate the new strategy of rhamnolipid production under denitrification ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/1097-0290(20010105)72:1<25::aid-bit4>3.0.c
更新日期:2001-01-05 00:00:00
abstract::The budding yeast Saccharomyces cerevisiae is equipped with G protein-coupled receptors (GPCR). Because the yeast GPCR signaling mechanism is partly similar to that of the mammalian system, S. cerevisiae can be used for a host of mammalian GPCR expression and ligand-mediated activation assays. However, currently avail...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26255
更新日期:2017-06-01 00:00:00
abstract::Oxygen and nutrient limitation was investigated in order to identify the origin of a lower specific ajmalicine production in Catharanthus roseus cultures at high cell densities in an induction medium. The effect of oxygen limitation was explored by comparing two identically aerated and agitated high cell density biore...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260440409
更新日期:1994-08-05 00:00:00
abstract::Knowledge of the aqueous phase methane concentration is critical to understanding and controlling process kinetics in methanotrophic bioreactors. Unfortunately since no dissolved methane probe is commercially available, this data must be obtained off-line by the time-consuming gas-liquid partition method. In this stud...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21050
更新日期:2006-12-05 00:00:00
abstract::Based upon the general characteristics of the optimal feed rate profiles presented in an earlier article, efficient computational algorithms have been developed for fed-batch fermentation processes described by four or less mass balance equations. These algorithms make computations of optimal substrate feed rate profi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280915
更新日期:1986-09-01 00:00:00
abstract::The immobilization of dextransucrase in Ca-alginate beads relies on the close association between dextran polymer and dextransucrase. However, high amounts of dextran in the enzyme preparation drastically limit the specific activity of the immobilized enzyme (4 U/mL of alginate beads). Moreover, even in the absence of...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1183
更新日期:2001-11-05 00:00:00
abstract::A drug-releasing model compound based on photosensitive acrylated ortho-nitrobenzylether (o-NBE) moiety and fluorescein was synthesized to demonstrate photolysis as a mechanism for drug release. Release of this model compound from a hydrogel network can be controlled with light intensity (5-20 mW/cm(2)), exposure dura...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22882
更新日期:2010-12-15 00:00:00
abstract::The rationale behind KISR's involvement in SCP is explained and emphasis towards the use of aerobic thermophilic/thermotolerant methanol-utilizing bacterial cultures is stressed. An attempt is made to correlate different pilot plant harvesting procedures for biomass recovery with product quality. The latter is express...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260260911
更新日期:1984-09-01 00:00:00
abstract::Clostridium difficile has emerged as a major cause of infectious diarrhea in hospitalized patients, with increasing mortality rate and annual healthcare costs exceeding $3 billion. Since C. difficile infections are associated with the use of antibiotics, there is an urgent need to develop treatments that can inactivat...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26029
更新日期:2016-12-01 00:00:00