Stratified microbial structure and activity within anode biofilm during electrochemically assisted brewery wastewater treatment.

abstract：:Microplates with integrated optical oxygen sensors are a new tool to study metabolic rates and enzyme activities. Precise measurements are possible only if oxygen exchange between the sample and the environment is known. In this study we quantify gas exchange in plastic microplates. Dissolved oxygen was detected using...

journal_title：Biotechnology and bioengineering

authors： Arain S,Weiss S,Heinzle E,John GT,Krause C,Klimant I

更新日期：2005-05-05 00:00:00

abstract：:The naturally existing microbial hosts can rarely satisfy industrial requirements, thus there has always been an intense effort in strain engineering to meet the needs of these bioprocesses. Here, in this work, we want to prove the concept that engineering global transcription factor cAMP receptor protein (CRP) of Esc...

journal_title：Biotechnology and bioengineering

authors： Zhang H,Chong H,Ching CB,Jiang R

更新日期：2012-05-01 00:00:00

abstract：:Start-up phenomena in microbial biokinetic assays are not captured by the most commonly used growth-related equations. In this study we propose a new respirometric experimental design to estimate intrinsic growth parameters that allow us to avoid these limitations without data omission, separate mathematical treatment...

journal_title：Biotechnology and bioengineering

authors： Seoane J,Sin G,Lardon L,Gernaey KV,Smets BF

更新日期：2010-01-01 00:00:00

abstract：:An experimental device was constructed to allow nearly simultaneous measurements to be made on temperature and gas composition at different depths in a solid-substrate fermentation bed. The time-dependent values of temperature, mol % O(2) and mol % CO(2) were measured at five positions in beds 6.35 cm (2.5 in.) deep. ...

journal_title：Biotechnology and bioengineering

authors： Rathbun BL,Shuler ML

更新日期：1983-04-01 00:00:00

abstract：:Hepatic steatosis is a process of abnormal lipid deposition within the liver cells, often caused by excessive alcohol uptake or obesity. A conventional in vitro model for hepatic steatosis uses a liver cell culture, treated with fatty acids and measures accumulation of lipids within the cells. This model does not reca...

journal_title：Biotechnology and bioengineering

authors： Lee SY,Sung JH

更新日期：2018-11-01 00:00:00

abstract：:The high hydrophobicity of polycyclic aromatic hydrocarbons (PAHs) strongly reduces their bioavailability in aged contaminated soils, thus limiting their bioremediation. The biodegradation of PAHs in soils can be enhanced by employing surface-active agents. However, chemical surfactants are often recalcitrant and exer...

journal_title：Biotechnology and bioengineering

authors： Fava F,Berselli S,Conte P,Piccolo A,Marchetti L

更新日期：2004-10-20 00:00:00

abstract：:Plasmid propagation in populations of unstable, binary fission recombinant organisms has been studied using a segregated, population balance mathematical model. Segregated models have the advantage of direct incorporation of basic information on mechanisms and kinetics of plasmid replication and segregation at the sin...

journal_title：Biotechnology and bioengineering

authors： Seo JH,Bailey JE

更新日期：1985-02-01 00:00:00

abstract：:The enzymology and kinetics of tyrosine phenol lyase (TPL) from Erwinia herbicola, and tyrosine decarboxylase (TDC) from Streptococcus faecalis have been investigated for potential use in a coimmobilized multienzyme biocatalytic system for the production of dopamine. In this multienzyme biotransformation using whole c...

journal_title：Biotechnology and bioengineering

authors： Anderson WA,Moo-Young M,Legge RL

更新日期：1992-03-25 00:00:00

abstract：:Mass balances were performed on an alkaline lysis operation for the primary recovery of supercoiled plasmid DNA as part of a process for plasmid gene preparation. Escherichia coli DH5alpha/pSVbeta was cultured in defined medium by fed-batch fermentation and harvested at the end of the exponential phase. Alkaline lysis...

journal_title：Biotechnology and bioengineering

authors： Ciccolini LA,Shamlou PA,Titchener-Hooker N

更新日期：2002-03-30 00:00:00

abstract：:For the commercial exploitation of lipase biocatalysis to be successful, it is essential that effective supports are selected for lipase immobilization. In this study hydrophobic controlled-pore glasses have been used as model systems for the immobilization of Rhizomucor miehei lipase. The effect of pore diameter and ...

journal_title：Biotechnology and bioengineering

authors： Bosley JA,Clayton JC

更新日期：1994-04-25 00:00:00

abstract：:There is strong evidence in the literature supporting the existence of significant mass transfer limitations on the kinetics of exogenous H(2) consumption by methanogens. The half saturation constant for H (2) uptake by a mixed-culture, CH(4) producing enrichment was measured using an experimental protocol that avoide...

journal_title：Biotechnology and bioengineering

authors： Giraldo-Gomez E,Goodwin S,Switzenbaum MS

更新日期：1992-10-05 00:00:00

abstract：:D-Methionine was converted to L-methionine in a reaction system where four enzymes were used. D-amino acid oxidase (D-AAO) from Arthrobacter protophormiae was used for the complete conversion of D-methionine to 2-oxo-4-methylthiobutyric acid. Catalase was added to prevent 2-oxo-4-methylthiobutyric acid decarboxylation...

journal_title：Biotechnology and bioengineering

authors： Findrik Z,Vasić-Racki D

更新日期：2007-12-01 00:00:00

abstract：:Rate and yield expressions relating to biomass and xanthan formation and to nitrogen, glucose, and oxygen consumption were established for Xanthomonas campestris batch fermentations in a bubble column. Microbial growth was described by the logistic rate equation, characterized by a maximum specific growth rate mu(M) =...

journal_title：Biotechnology and bioengineering

authors： Pons A,Dussap CG,Gros JB

更新日期：1989-01-20 00:00:00

abstract：:Co-culture of hepatocytes or hepatocyte spheroids with the supporting NIH3T3 in a 3D microcapsule formed with a hybrid natural/synthetic matrix has led to enhanced hepatocyte functions. We investigated the mechanism of the functional enhancement in co-culture with respect to the contributions of soluble factors and di...

journal_title：Biotechnology and bioengineering

authors： Chia SM,Lin PC,Yu H

更新日期：2005-03-05 00:00:00

abstract：:Lutein and zeaxanthin are dietary carotenoids reported to be protective against age-related macular degeneration. Recently, the green alga Chlamydomonas reinhardtii has received attention as a photosynthetic cell factory, but the potential of this alga for carotenoid production has not yet been evaluated. In this stud...

journal_title：Biotechnology and bioengineering

authors： Baek K,Yu J,Jeong J,Sim SJ,Bae S,Jin E

更新日期：2018-03-01 00:00:00

abstract：:A major challenge in downstream purification of monoclonal antibodies (mAb) is the removal of host cell proteins (HCPs). Previous studies have shown that cell culture conditions significantly impact the HCP content at harvest. However, it is currently unclear how process conditions affect physiological changes in the ...

journal_title：Biotechnology and bioengineering

authors： Goey CH,Tsang JMH,Bell D,Kontoravdi C

更新日期：2017-12-01 00:00:00

abstract：:10-Deacetylabaccatin III (10 DAB), an important precursor for paclitaxel semisynthesis, is enhanced in yew extracts using C10-deacetylase and C13-deacylase enzymes.(4) C10-deacetylase is an intracellular enzyme produced by the fermentation of a soil microorganism, Nocardioides luteus (SC 13912). During the fermentatio...

journal_title：Biotechnology and bioengineering

authors： Nanduri VB,Hanson RL,Laporte TL,Ko RY,Patel RN,Szarka LJ

更新日期：1995-12-05 00:00:00

abstract：:We propose an in vitro selection strategy to identify bacteriophage variants that recognize metal ions in solution. In 6 M urea, phage T7 loses 99.9% of its activity in less than 5 min. Inactivation is accelerated by gold, but slowed by zinc and magnesium. Selection of phage over five generations in the presence of go...

journal_title：Biotechnology and bioengineering

authors： Gupta K,Yin J

更新日期：1995-03-05 00:00:00

abstract：:A comprehensive study of the application of continuous zone electrophoresis to preparative separation of proteins in free solution is presented. First, the influence of electric field strength, buffer residence time in the chamber, sample flow rate, and sample concentration on separation resolution and throughput were...

journal_title：Biotechnology and bioengineering

authors： Nath S,Schütte H,Hustedt H,Deckwer WD

更新日期：1993-09-20 00:00:00

abstract：:Using phage display technology, we have isolated 12-mer peptide ligands that bind to human blood outgrowth endothelial cells (HBOEC). To avoid non-specific binding we apply negative-positive selection approach by pre-incubating the library with non-HBOEC. The selected phage clones bind to their target cell population ...

journal_title：Biotechnology and bioengineering

authors： Veleva AN,Cooper SL,Patterson C

更新日期：2007-09-01 00:00:00

abstract：:By investigating the effects of four operating variables-volume (V), Ultrafiltration flux (J), enzyme concentration (E), and substrate concentration (S)-on capacity (K) and conversion rate (epsilon) of a hollow fiber CSTR, the performances of the CSTR and the kinetic constants of the reaction were determined. A model ...

journal_title：Biotechnology and bioengineering

authors： Bressollier P,Petit JM,Julien R

更新日期：1988-05-01 00:00:00

abstract：:The effects of dilution rates on the performance of a two-stage fermentation system for a recombinant Escherichia coli culture were studied. Dilution rate determines the apparent or averaged specific growth rate of a heterogeneous population of cells in the recombinant culture. The specific growth rate affects the gen...

journal_title：Biotechnology and bioengineering

authors： Hortacsu A,Ryu DD

更新日期：1991-10-20 00:00:00

abstract：:Regenerating damaged tissue interfaces remains a significant clinical challenge, requiring recapitulation of the structure, composition, and function of the native enthesis. In the ligament-to-bone interface, this region transitions from ligament to fibrocartilage, to calcified cartilage and then to bone. This gradati...

journal_title：Biotechnology and bioengineering

authors： Harris E,Liu Y,Cunniffe G,Morrissey D,Carroll S,Mulhall K,Kelly DJ

更新日期：2017-10-01 00:00:00

abstract：:The goal of this work was to measure and model the effect of thermal exposure on the fluorescence emission of R-phycoerythrin (R-PE). The long-term objective of our work is to assess the feasibility of encapsulating R-PE for use as the critical component of a time-temperature integrator (TTI) for ascertaining the degr...

journal_title：Biotechnology and bioengineering

authors： Vaidya S,Orta-Ramirez A,Smith DM,Ofoli RY

更新日期：2003-08-20 00:00:00

abstract：:This article describes a method for the chemical immobilization of concanavalin A (Con A) on the inside wall of a single hollow cellulose fiber for use in glucose affinity sensor. Periodate oxidation of cellulose fiber followed by a spacer for Con A attachment was deemed to be the most optimal procedure for achieving ...

journal_title：Biotechnology and bioengineering

authors： Srinivasan KR,Mansouri S,Schultz JS

更新日期：1986-02-01 00:00:00

abstract：:Coastal bermudagrass was pretreated by a low-temperature ammonia fiber explosion (AFEX) process, which soaked the grass in liquid ammonia and then explosively released the pressure. Saccharifying enzymes were systematically applied to the AFEX-treated grass corresponding to low, medium, and high loadings of cellulase/...

journal_title：Biotechnology and bioengineering

authors： Holtzapple MT,Ripley EP,Nikolaou M

更新日期：1994-11-05 00:00:00

abstract：:An immobilized enzyme reactor has been developed to remove heparin, the anticoagulant that is required in all extracorporeal devices for patients undergoing open-heart surgery or kidney dialysis. The device uses the enzyme heparinase (EC 4.2.2.7), which is covalently linked to agarose with cyanogen bromide. A critical...

journal_title：Biotechnology and bioengineering

authors： Bernstein H,Yang VC,Langer R

更新日期：1987-08-05 00:00:00

abstract：:Targeted gene integration into site-specific loci can be achieved in Chinese hamster ovary (CHO) cells via CRISPR/Cas9 genome editing technology and the homology-directed repair (HDR) pathway. The low efficiency of HDR often requires antibiotic selection, which limits targeted integration of multiple genes at multiple...

journal_title：Biotechnology and bioengineering

authors： Lee JS,Grav LM,Pedersen LE,Lee GM,Kildegaard HF

更新日期：2016-11-01 00:00:00

abstract：:We studied the resolution of racemic glycidol through esterification with butyric acid catalyzed by porcine pancreatic lipase in organic media. A screening of seven solvents (log P values between 0.49 and 3.0, P being the n-octanol-water partition coefficient of the solvent) showed that neither log P nor the logarithm...

journal_title：Biotechnology and bioengineering

authors： Martins JF,Da Ponte MN,Barreiros S

更新日期：1993-08-05 00:00:00

abstract：:The combination of Sephadex G-15 and ion-exchange resin columns allows one-step desalting and separation of cellodextrins using water as the sole eluent. The column apparatus described in this paper has the potential of producing up to 3 g cellodextrins in one day. In addition, the columns described are stable and do ...

journal_title：Biotechnology and bioengineering

authors： Markovits A

更新日期：1978-10-01 00:00:00