Saccharification, fermentation, and protein recovery from low-temperature AFEX-treated coastal bermudagrass.

abstract：:Macroencapsulation technology has been an attractive topic in the field of treatment for Type 1 diabetes due to mechanical stability, versatility, and retrievability of the macro-capsule design. Macro-capsules can be categorized into extravascular and intravascular devices, in which solute transport relies either on d...

journal_title：Biotechnology and bioengineering

authors： Song S,Roy S

更新日期：2016-07-01 00:00:00

abstract：:The hydrolysis of lactose by a beta-galactosidase from the thermophilic microorganism Thermus sp. strain T2, both in solution and immobilized on a commercial silica-alumina, has been studied. The enzyme has been previously produced by Escherichia coli JM101 harboring the plasmid pBGT1, which contains the codifying gen...

journal_title：Biotechnology and bioengineering

authors： Ladero M,Perez MT,Santos A,Garcia-Ochoa F

更新日期：2003-01-20 00:00:00

abstract：:Reduction and oxidation of steroids in the human gut are catalyzed by hydroxysteroid dehydrogenases of microorganisms. For the production of 12-ketochenodeoxycholic acid (12-Keto-CDCA) from cholic acid the biocatalytic application of the 12α-hydroxysteroid dehydrogenase of Clostridium group P, strain C 48-50 (HSDH) is...

journal_title：Biotechnology and bioengineering

authors： Braun M,Link H,Liu L,Schmid RD,Weuster-Botz D

更新日期：2011-06-01 00:00:00

abstract：:Glial cell-line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor known to enhance motor nerve regeneration following its delivery. However, recent studies have determined that extended GDNF delivery to regenerating axons can entrap motor axons at the site of GDNF delivery. This entrapment leads to re...

journal_title：Biotechnology and bioengineering

authors： Wu-Fienberg Y,Moore AM,Marquardt LM,Newton P,Johnson PJ,Mackinnon SE,Sakiyama-Elbert SE,Wood MD

更新日期：2014-09-01 00:00:00

abstract：:Plasmid gene product accumulation in a cell population depends on the fraction of plasmid-containing cells and the distribution of single-cell plasmid content. These important population properties have been related to plasmid replication regulation and kinetics and to plasmid segregation rules at the single-cell leve...

journal_title：Biotechnology and bioengineering

authors： Hjortso MA,Bailey JE

更新日期：1984-05-01 00:00:00

abstract：:The influence of solution Eh on the rate of ferrous iron oxidation by Acidithiobacillus ferrooxidans is characterized. The experimental approach was based on the use of a two-chamber bioelectrochemical cell, which can determine the ferrous iron oxidation rate at controlled potential. Results enabled the formulation of...

journal_title：Biotechnology and bioengineering

authors： Meruane G,Salhe C,Wiertz J,Vargas T

更新日期：2002-11-05 00:00:00

abstract：:Reactive oxygen species (ROS), encompassing all oxygen radical or non-radical oxidizing agents, play key roles in disease progression. Controlled delivery of antioxidants is therapeutically relevant in such oxidant-stressed environments. Encapsulating small hydrophilic molecules into hydrophobic polymer microparticles...

journal_title：Biotechnology and bioengineering

authors： Murphy NP,Lampe KJ

更新日期：2018-01-01 00:00:00

abstract：:Recently, we reported that the loss of a telomeric region of chromosome 8 in Chinese Hamster Ovary (CHO) cells correlates with higher recombinant productivities. New cell lines lacking this region, called CHO-C8DEL, showed several advantages during cell line generation and for the production of recombinant proteins (R...

journal_title：Biotechnology and bioengineering

authors： Ritter A,Rauschert T,Oertli M,Piehlmaier D,Mantas P,Kuntzelmann G,Lageyre N,Brannetti B,Voedisch B,Geisse S,Jostock T,Laux H

更新日期：2016-11-01 00:00:00

abstract：:A recently discovered thermophilic bacterium, Geobacillus thermoglucosidasius M10EXG, ferments a range of C5 (e.g., xylose) and C6 sugars (e.g., glucose) and is tolerant to high ethanol concentrations (10%, v/v). We have investigated the central metabolism of this bacterium using both in vitro enzyme assays and 13C-ba...

journal_title：Biotechnology and bioengineering

authors： Tang YJ,Sapra R,Joyner D,Hazen TC,Myers S,Reichmuth D,Blanch H,Keasling JD

更新日期：2009-04-01 00:00:00

abstract：:A semiequilibrium model of chromatography has been developed to account for the elution profile of high concentration bands. The use of this model requires the prior determination of the equilibrium isotherms of the studied compounds in the chromatographic phase system. The model is applied here to a study of the isoc...

journal_title：Biotechnology and bioengineering

authors： Katti A,Huang JX,Guiochon G

更新日期：1990-07-01 00:00:00

abstract：:By feeding ethanol at various high rates to low cell density cultures of Saccharomyces cerevisiae it was shown that the sharp fall in viability when ethanol is produced during rapid fermentations is in part a direct consequence of the high rate of change of extracellular ethanol concentration. Nevertheless, the fall i...

journal_title：Biotechnology and bioengineering

authors： Dasari G,Worth MA,Connor MA,Pamment NB

更新日期：1990-01-20 00:00:00

abstract：:A uniquely simple model is developed to describe the NO(3) (-) and NO(2) (-) concentration profiles within a dentrification fluidized bed biological reactor. This simple model is compared to experimental data, and to a more complex model similar to those previously proposed in the literature. The simple model fits the...

journal_title：Biotechnology and bioengineering

authors： Brosilow BJ,Schnitzer M,Tarre S,Green M

更新日期：1997-06-20 00:00:00

abstract：:The functional analysis of plant cells at the cellular and subcellular levels requires novel technologies for the directed manipulation of individual cells. In this report, we demonstrate the use of an infrared (1,064 nm) picosecond laser for the perforation of tobacco cell protoplasts. A single pulse was sufficient t...

journal_title：Biotechnology and bioengineering

authors： Schinkel H,Jacobs P,Schillberg S,Wehner M

更新日期：2008-01-01 00:00:00

abstract：:Numerous biotechnological production processes are based on the submerse cultivation of filamentous fungi. Process design, however, is often hampered by the complex growth pattern of these organisms. In the morphologic development of coagulating filamentous fungi, like Aspergillus niger, conidial aggregation is the fi...

journal_title：Biotechnology and bioengineering

authors： Lin PJ,Grimm LH,Wulkow M,Hempel DC,Krull R

更新日期：2008-02-01 00:00:00

abstract：:In this study, omics-based analysis tools were used to explore the effect of glucose starvation and culture duration on monoclonal antibody (mAb) production in fed-batch CHO cell culture to gain better insight into how these parameters can be controlled to ensure optimal mAb productivity and quality. Titer and N-glyco...

journal_title：Biotechnology and bioengineering

authors： Fan Y,Jimenez Del Val I,Müller C,Lund AM,Sen JW,Rasmussen SK,Kontoravdi C,Baycin-Hizal D,Betenbaugh MJ,Weilguny D,Andersen MR

更新日期：2015-10-01 00:00:00

abstract：:Nanoparticles (NPs) are able to deliver a variety of substances into eukaryotic cells. However, their usage is often hampered by a lack of specificity, leading to the undesired uptake of NPs by virtually all cell types. In contrast to this, yeast is known to be specifically taken up into immune cells after entering th...

journal_title：Biotechnology and bioengineering

authors： Kiefer R,Jurisic M,Dahlem C,Koch M,Schmitt MJ,Kiemer AK,Schneider M,Breinig F

更新日期：2020-03-01 00:00:00

abstract：:Fatty aldehydes and alcohols are valuable precursors used in the industrial manufacturing of a myriad of specialty products. Herein, we demonstrate the de novo production of odd chain-length fatty aldehydes and fatty alcohols in Saccharomyces cerevisiae by expressing a novel biosynthetic pathway involving cytosolic th...

journal_title：Biotechnology and bioengineering

authors： Jin Z,Wong A,Foo JL,Ng J,Cao YX,Chang MW,Yuan YJ

更新日期：2016-04-01 00:00:00

abstract：:Relatively conservative modifications of three proteins were carried out to alter their surface properties. The protein properties modified were hydrophobicity and charge. This was done by acylation of amino groups with anhydrides. For the hydrophobic modification experiments, two proteins (beta-lactoglobulin and bovi...

journal_title：Biotechnology and bioengineering

authors： Franco TT,Andrews AT,Asenjo JA

更新日期：1996-02-05 00:00:00

abstract：:The feasibility of NO3- removal by the synergistic action of a prevailing denitrifying anoxic methane oxidising (DAMO), and nitrate-reducing and sulfide-oxidising bacterial (NR-SOB) consortium, using CH4 and H2 S from biogas as electron donors in a biotrickling filter was investigated. The influence of NO3- concentrat...

journal_title：Biotechnology and bioengineering

authors： López JC,Porca E,Collins G,Pérez R,Rodríguez-Alija A,Muñoz R,Quijano G

更新日期：2017-03-01 00:00:00

abstract：:Highly substituted polychlorinated biphenyls (PCBs) are known to be very resistant to aerobic biodegradation, particularly the initial attack by biphenyl dioxygenase. Functional evolution of the substrate specificity of biphenyl dioxygenase was demonstrated by DNA shuffling and staggered extension process (StEP) of th...

journal_title：Biotechnology and bioengineering

authors： Brühlmann F,Chen W

更新日期：1999-06-05 00:00:00

abstract：:Aspergillus awamori NRRL 4869 was cultured on the solid substrate, wheat bran, in a modified Rollacell apparatus to produce alpha-galactosidase and invertase. The swivel cap on the elongated bottle permits the introduction of air while the bottle rotates. Parameters of air flow rate (0.05-0.2 liter/kg/min), rpm (0.15-...

journal_title：Biotechnology and bioengineering

authors： Silman RW

更新日期：1980-02-01 00:00:00

abstract：:The cellulase system of Bacteroides cellulosolvens was subjected to both catabolite repression and feedback inhibition by cellobiose. Cellulose-solubilizing activity was 50% inhibited at a cellobiose concentration of 2.6 g/L and completely inhibited by 12 g/L. Glucose at 12 g/L (the highest concentration tested) had n...

journal_title：Biotechnology and bioengineering

authors： Murray WD

更新日期：1987-06-01 00:00:00

abstract：:Breast cancer cells experience a range of shear stresses in the tumor microenvironment (TME). However most current in vitro three-dimensional (3D) models fail to systematically probe the effects of this biophysical stimuli on cancer cell metastasis, proliferation, and chemoresistance. To investigate the roles of shear...

journal_title：Biotechnology and bioengineering

authors： Novak CM,Horst EN,Taylor CC,Liu CZ,Mehta G

更新日期：2019-11-01 00:00:00

abstract：:Effective enzyme-mediated viscosity reduction, disaggregation, or "liquefaction," is required to overcome the rheological challenges resulting from the fibrous, hygroscopic nature of lignocellulosic biomass, particularly at the high solids loadings that will be required for an economically viable process. However, the...

journal_title：Biotechnology and bioengineering

authors： van der Zwan T,Hu J,Saddler JN

更新日期：2017-11-01 00:00:00

abstract：:Installing membranes for solid-liquid separation into biological nutrient removal (BNR) activated sludge (AS) systems makes a profound difference not only in the design of the BNR system itself, but also in the design approach for the whole wastewater treatment plant (WWTP). In multizone BNR systems with membranes in ...

journal_title：Biotechnology and bioengineering

authors： Ramphao M,Wentzel MC,Merritt R,Ekama GA,Young T,Buckley CA

更新日期：2005-03-20 00:00:00

abstract：:Three cell lines, that is, the human breast cancer cell line (MCF-7) and the human mammary epithelial cell line (S-1) and its malignant form (T4-2) were embedded in a reconstituted basement membrane (Matrigel) that had 20-nL pyramid-shaped silicon microstructures. The proliferative behavior of the MCF-7 cells was depe...

journal_title：Biotechnology and bioengineering

authors： Torisawa YS,Nashimoto Y,Yasukawa T,Shiku H,Matsue T

更新日期：2007-06-15 00:00:00

abstract：:Gene deletion strategies using flux balance analysis (FBA) have improved the growth-coupled production of various compounds. However, the productivities were often below the expectation because the cells failed to adapt to these genetic perturbations. Here, we demonstrate the productivity of the succinate of the desig...

journal_title：Biotechnology and bioengineering

authors： Tokuyama K,Toya Y,Horinouchi T,Furusawa C,Matsuda F,Shimizu H

更新日期：2018-06-01 00:00:00

abstract：:Membrane morphology is compared to protein depostion under passive adsorption and ultrafiltration conditions. Solute resistance of protein deposits for membranes of varying roughness, structure, and permeability can vary dramatically with operating conditions. Using Brunauer-Emmett-Teller adsorption isotherm (BET), st...

journal_title：Biotechnology and bioengineering

authors： Chen V,Kim KJ,Fane AG

更新日期：1995-07-20 00:00:00

abstract：:The stability of immobilized preparations of xanthine oxidase and urate oxidase was studied, and optimized, because of the potential joint use of both enzymes in clinical analysis. Xanthine oxidase was immobilized on cellulose, Sepharose, hornblende, Enzacryl-TIO, and porous glass. Thehalf-lives of these preparations ...

journal_title：Biotechnology and bioengineering

authors： Johnson DB,Coughlan MP

更新日期：1978-07-01 00:00:00

abstract：:Zwittermicin A (ZwA), a hybrid polyketide- peptide antibiotic, and N-acyl -homoserine-lactonase (AHL-lactonase), a kind of quorum-quenching enzyme, have the potential to prevent the virulence of Erwinia carotovora, which is a major pathogen of soft rot. The purpose of this study was to combine the two agents in one ho...

journal_title：Biotechnology and bioengineering

authors： Zhao C,Zeng H,Yu Z,Sun M

更新日期：2008-06-15 00:00:00