Abstract:
:The functional analysis of plant cells at the cellular and subcellular levels requires novel technologies for the directed manipulation of individual cells. In this report, we demonstrate the use of an infrared (1,064 nm) picosecond laser for the perforation of tobacco cell protoplasts. A single pulse was sufficient to perforate the plasma membrane enabling the uptake of dye from the surrounding medium into the cytosol. Moreover, the procedure was shown to be suitable for the efficient delivery of DNA expression constructs to the nucleus, as demonstrated by the subsequent expression and correct targeting of a recombinant fluorescent protein. Single cell perforation using this novel optoporation method shows that isolated plant cells can be permeabilized without direct manipulation. This is a valuable procedure for cell-specific applications, particularly where the import of specific molecules into plant cells is required for functional analysis.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Schinkel H,Jacobs P,Schillberg S,Wehner Mdoi
10.1002/bit.21549subject
Has Abstractpub_date
2008-01-01 00:00:00pages
244-8issue
1eissn
0006-3592issn
1097-0290journal_volume
99pub_type
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