Abstract:
:The feasibility of NO3- removal by the synergistic action of a prevailing denitrifying anoxic methane oxidising (DAMO), and nitrate-reducing and sulfide-oxidising bacterial (NR-SOB) consortium, using CH4 and H2 S from biogas as electron donors in a biotrickling filter was investigated. The influence of NO3- concentration on N2 O production during this process was also evaluated. The results showed that NO3- was removed at rates up to 2.8 g mreactor-3 h-1 using CH4 as electron donor. N2 O production rates correlated with NO3- concentration in the liquid phase, with a 10-fold increase in N2 O production as NO3- concentration increased from 50 to 200 g m-3 . The use of H2 S as co-electron donor resulted in a 13-fold increase in NO3- removal rates (∼18 gNO3- m-3 h-1 ) and complete denitrification under steady-state conditions, which was supported by higher abundances of narG, nirK, and nosZ denitrifying genes. Although the relative abundance of the DAMO population in the consortium was reduced from 60% to 13% after H2 S addition, CH4 removals were not compromised and H2 S removal efficiencies of 100% were achieved. This study confirmed (i) the feasibility of co-oxidising CH4 and H2 S with denitrification, as well as (ii) the critical need to control NO3- concentration to minimize N2 O production by anoxic denitrifiers. Biotechnol. Bioeng. 2017;114: 665-673. © 2016 Wiley Periodicals, Inc.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
López JC,Porca E,Collins G,Pérez R,Rodríguez-Alija A,Muñoz R,Quijano Gdoi
10.1002/bit.26092subject
Has Abstractpub_date
2017-03-01 00:00:00pages
665-673issue
3eissn
0006-3592issn
1097-0290journal_volume
114pub_type
杂志文章abstract::The ability to capture cell-free DNA from the gastrointestinal tract, in a minimally invasive manner, could enhance our ability to diagnose gastrointestinal disease, or gain a better understanding of the spatial mapping of the intestinal microbiota. We, therefore, sought to identify a class of capture agents that coul...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26207
更新日期:2017-04-01 00:00:00
abstract::The direct microbial conversion (DMC) process for the production of ethanol from lignocellulosic biomass is limited by low volumetric ethanol production rates due to the low cell densities of Clostridium thermosaccharolyticum which is a key organism for ethanol production in this process. Hence, this study focuses on ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260341008
更新日期:1989-12-05 00:00:00
abstract::Extractive work-up of whole-cell biotransformations generally suffers from the formation of stable gels and slimes upon addition of the organic solvent to the cell suspension and the cell-free solution, respectively. This problem has been overcome by enzymatic lysis of emulsifying agents present in the medium through ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20155
更新日期:2004-08-20 00:00:00
abstract::Current animal and 2-D cell culture models employed in metastasis research and drug discovery remain poor mimics of human cancer physiology. Here we describe a "metastasis-on-a-chip" system allowing real time tracking of fluorescent colon cancer cells migrating from hydrogel-fabricated gut constructs to downstream liv...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25950
更新日期:2016-09-01 00:00:00
abstract::An integrated approach is used to develop a rapid sampling strategy for the quantitative analysis of in vivo kinetic behavior based on measured concentrations of intracellular metabolites in Saccharomyces cerevisiae. Emphasis is laid on small sample sizes during sampling and analysis. Subsecond residence times are acc...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10048
更新日期:2001-11-20 00:00:00
abstract::During continuous lipase-catalyzed oleic acid esterification by ethanol in n-hexane, the oleic acid conversion, initially at 95%, decreases to 20% after 2 h. This decrease is caused by the accumulation of the water produced in the course of the reaction in the packed-bed reactor (PBR). In order to improve the PBR effi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1998-11-05 00:00:00
abstract::Chemostat cultivation of Aspergillus niger and other filamentous fungi is often hindered by the spontaneous appearance of morphologic mutants. Using the Variomixing bioreactor and applying different chemostat conditions we tried to optimize morphologic stability in both ammonium- and glucose-limited cultures. In most ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20046
更新日期:2004-05-05 00:00:00
abstract::The addition of simple inorganic salts to aqueous enzyme solutions prior to lyophilization results in a dramatic activation of the dried powder in organic media relative to enzyme with no added salt. Activation of both the serine protease subtilisin Carlsberg and lipase from Mucor javanicus resulting from lyophilizati...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990420)63:2<233::aid-bit
更新日期:1999-04-20 00:00:00
abstract::A new method is presented to precipitate proteins and amino acids from reverse micelles by dehydrating the micelles with molecular sieves. Nearly complete precipitation is demonstrated for alpha-chymotrypsin, cytochromec, and trytophan from 2-ethylhexyl sodium sulfosuccinate (AOT)/isooctane/water reverse micelle solut...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260440708
更新日期:1994-09-20 00:00:00
abstract::The influence of biofilm areal cell density, species composition, and the presence of abiotic particles on the disinfection and removal of bacterial biofilms by monochloramine was investigated. Mono- and binary population biofilms of Pseudomonas aeruginosa and Klebsiella pneumoniae were grown on stainless-steel slides...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260460608
更新日期:1995-06-20 00:00:00
abstract::A nuclear magnetic resonance (NMR) method has been developed to monitor on-line lipase-catalyzed esterification reactions without the need to sample the reaction medium. The technique, through (1)H NMR, measures the concentrations of alcohol, ester, hydroxylic hydrogens in the organic phase, and hydroxylic hydrogens i...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960920)51:6<636::AID-BIT
更新日期:1996-09-20 00:00:00
abstract::A stimulus-response tracer technique has been used to characterize packed beds of untreated, as well as acid prehydrolyzed, and enzymatically hydrolyzed aspen wood chips. Glucose was used as the tracer. Bulk liquid phase dispersion, interphase mass transfer, and intraparticle diffusion coefficients were determined for...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260410310
更新日期:1993-02-05 00:00:00
abstract::A set of eight closely related plasmid constructs carrying CI857-controlled recombinant genes has been used as a model to study plasmid stability in Escherichia coli, in the absence of antibiotic selection. Plasmid loss rates and relative interdivision times of plasmid-bearing cells and plasmid-free cells have been an...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1998-06-20 00:00:00
abstract::Synthetic mixtures of predominant lignocellulosic hexose sugars were supplemented with separate aliquots of three inhibitory compounds (furfural, hydroxymethylfurfural (HMF), and acetic acid) in a series of concentrations and fermented by the spent sulfite liquor (SSL)-adapted yeast strain Tembec T1 and the natural is...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20838
更新日期:2006-04-20 00:00:00
abstract::The relationship between local mass transfer coefficient and fluid velocity in heterogenous biofilms was investigated by combining microelectrodes and confocal scanning laser microscopy (CSLM). The biofilms were grown for up to 7 days and consisted of cell clusters separated by interstitial channels. Mass transfer coe...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19971220)56:6<681::AID-BIT
更新日期:1997-12-20 00:00:00
abstract::The bacterial consortium MPD-M, isolated from sediment associated with Colombian mangrove roots, was effective in the treatment of hydrocarbons in water with salinities varying from 0 to 180 g L(-1). Where the salinity of the culture medium surpassed 20 g L(-1), its effectiveness increased when the cells were immobili...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10318
更新日期:2002-07-20 00:00:00
abstract::Poly lactic acid (PLA) based plastics is renewable, bio-based, and biodegradable. Although present day PLA is composed of mainly L-LA, an L- and D- LA copolymer is expected to improve the quality of PLA and expand its use. To increase the number of thermotolerant microbial biocatalysts that produce D-LA, a derivative ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26472
更新日期:2018-02-01 00:00:00
abstract::Murine hybridoma cells were grown in a bubble column that was inclined up to 45 degrees from vertical. Inclining the column by a few degrees separated the rising bubbles against the upper surface, leaving the bulk of the liquid bubble free. The liquid was circulated well by the rising bubbles, but collection of cells ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260450212
更新日期:1995-01-20 00:00:00
abstract::A low-density, porous chitosan/poly-(dl-lactide-co-glycolide) (PLGA) microparticle composite scaffold was produced by thermally induced phase separation followed by lyophilization, to provide a bicontinuous microstructure potentially suitable for tissue engineering and locally controlled drug release. PLGA particles w...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21796
更新日期:2008-07-01 00:00:00
abstract::Biosorption of elements is a little understood phenomenon exhibited by some types of even nonliving microbial biomass. A common fungus Rhizopus arrhizus has been reported to take up uranium from aqueous solutions to the extent of 180 mg U(6+)/g. The mechanism of uranium sequestering by this type of biomass was studied...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260240211
更新日期:1982-02-01 00:00:00
abstract::Adsorption of cellulases onto lignin is considered a major factor in retarding enzymatic cellulose degradation of lignocellulosic biomass. However, the adsorption mechanisms and kinetics are not well understood for individual types of cellulases. This study examines the binding affinity, kinetics of adsorption, and co...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26820
更新日期:2018-12-01 00:00:00
abstract::A mechanistically detailed single-cell model E. coli B/r-A was adapted to simulate the effects of vector presence on cell metabolism. Competition for RNA polymerase between chromosome and plasmid DNA is explicitly included. Distribution of active ribosomes among chromosome- and plasmid-derived messenger RNA, another k...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260290305
更新日期:1987-02-01 00:00:00
abstract::Application of plasmid DNA as pharmaceutical to be used in gene therapy and vaccination has been investigated intensively in recent years. To be able to provide sufficient material that is in accordance with quality of pharmaceutical grade it is mandatory to gain comprehensive process knowledge which is even requested...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23138
更新日期:2011-09-01 00:00:00
abstract::Frozen bagged seed inoculum was prepared, thawed and tested for seven cultures. Thawing techniques were developed and other key influences on thawing rate were quantified; seed bag thawing without a water bath rarely required more than 4 to 5 h and was as short as 0.5 to 1 h for lower fill volume bags. Testing include...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10323
更新日期:2002-09-20 00:00:00
abstract::The effect of organic solvents on the equilibrium position of lipase-catalyzed esterification of glycerol and decanoic acid has been investigated. The reaction is carried out in an aqueous-organic two-phase system. In polar solvents, high mole fractions of monoacylglycerol and low mole fractions of triacylglycerol and...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260410113
更新日期:1993-01-05 00:00:00
abstract::The development of renewable alternatives to diesel and jet fuels is highly desirable for the heavy transportation sector, and would offer benefits over the production and use of short-chain alcohols for personal transportation. Here, we report the development of a metabolically engineered strain of Escherichia coli t...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22660
更新日期:2010-06-01 00:00:00
abstract::Remarkable increases in enzyme catalytic stability resulting from addition of charged water-soluble polymers have recently been reported, suggesting that use of these polymers may be an attractive general strategy for enzyme stabilization. To test the proposed hypothesis that coulombic forces between water-soluble pol...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1187
更新日期:2001-11-01 00:00:00
abstract::Pancreatic islet transplantation is a promising alternative to whole-pancreas transplantation as a treatment of type 1 diabetes mellitus. This technique has been extensively developed during the past few years, with the main purpose of minimizing the complications arising from the standard protocols used in organ tran...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章,评审
doi:10.1002/bit.26821
更新日期:2018-12-01 00:00:00
abstract::Microbial degradation of trichloroethylene (TCE) has been demonstrated under aerobic conditions with propane. The primary objective of this research was to evaluate the feasibility of introducing a vapor phase form of TCE in the presence of propane to batch bioreactors containing a liquid phase suspension of Mycobacte...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260460406
更新日期:1995-05-20 00:00:00
abstract::Alkyl beta-D-glucosides were synthesized from D-glucose and alcohols by reverse hydrolysis using the commercially available almond beta-D-glucosidase in 9:1 (v/v) acetonitrile-water medium. The main characteristics of this enzyme-catalyzed glucosylation were established by using 2-hydroxybenzyl alcohol. The reaction i...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260460204
更新日期:1995-04-20 00:00:00