A simple model describing nitrate and nitrite reduction in fluidized bed biological reactors.

abstract：:The effects of external calcium concentrations on biosynthesis of ginsenoside Rb1 and several calcium signal sensors were quantitatively investigated in suspension cultures of Panax notoginseng cells. It was observed that the synthesis of intracellular ginsenoside Rb1 in 3-day incubation was dependent on the medium Ca...

journal_title：Biotechnology and bioengineering

authors： Yue CJ,Zhong JJ

更新日期：2005-02-20 00:00:00

abstract：:Catalytic membranes, obtained by immobilizing thermophilic beta-glycosidase onto nylon supports, were used in a nonisothermal bioreactor to study the effect of temperature gradients on the rate of enzyme reaction. Two experimental approaches were carried out to explain the molecular mechanisms by which the temperature...

journal_title：Biotechnology and bioengineering

authors： Febbraio F,Portaccio M,Stellato S,Rossi S,Bencivenga U,Nucci R,Rossi M,Gaeta FS,Mita DG

更新日期：1998-07-05 00:00:00

abstract：:Static and dynamic characteristics of continuous cultures of recombinant methylotrophs, which are designed to improve the selectivity of plasmid-bearing cells and the plasmid stability, are investigated in detail. Operational regions in which coexistence (survival of plasmid-bearing and plasmid-free cells) operation i...

journal_title：Biotechnology and bioengineering

authors： Parulekar SJ,Chang YK,Modak JM,Lim HC

更新日期：1987-06-01 00:00:00

abstract：:Recent studies have demonstrated the feasibility of using ultrafiltration for the purification of plasmid DNA, but there is still little understanding of the factors governing DNA transmission. Experimental data were obtained for the transmission of a 3.0 kbp supercoiled plasmid DNA through composite regenerated cellu...

journal_title：Biotechnology and bioengineering

authors： Latulippe DR,Zydney AL

更新日期：2008-02-01 00:00:00

abstract：:Gene expression circuitries with time-delayed expression profiles regulate key events, such as oscillating systems, noise elimination, and coordinated multi-step processes, in all organisms from bacteria to mammalian cells. We present the rational synthesis of a genetic circuit displaying time-delayed expression in si...

journal_title：Biotechnology and bioengineering

authors： Weber W,Kramer BP,Fussenegger M

更新日期：2007-11-01 00:00:00

abstract：:A balance between proliferation and cell death is critical for achieving desirable high cell densities in mammalian cell culture. In this study, we evaluate a recently discovered anti-apoptotic gene, aven, and examine its effectiveness alone and in combination with a member of the Bcl-2 family, bcl-xL. The commerciall...

journal_title：Biotechnology and bioengineering

authors： Figueroa B Jr,Chen S,Oyler GA,Hardwick JM,Betenbaugh MJ

更新日期：2004-03-20 00:00:00

abstract：:The marine diatom Cyclotella cryptica was grown over a period of 13 months in a 48-m(2) shallow outdoor flume. The use of foil arrays at intervals of 1.2 m to effect systematic vertical mixing in the flume was found to significantly enhance microalgal production (p = 0.006). Average photosynthetic efficiencies (based ...

journal_title：Biotechnology and bioengineering

authors： Laws EA,Taguchi S,Hirata J,Pang L

更新日期：1988-07-05 00:00:00

abstract：:The development of new gene delivery technologies is a prerequisite towards gene therapy clinical trials. Because gene delivery mediated by viral vectors remains of limited scope due to immunological and propagation risks, the development of new non-viral gene delivery systems is of crucial importance. We have synthes...

journal_title：Biotechnology and bioengineering

authors： Byk G,Soto J,Mattler C,Frederic M,Scherman D

更新日期：1998-04-01 00:00:00

abstract：:The goal of this work was to measure and model the effect of thermal exposure on the fluorescence emission of R-phycoerythrin (R-PE). The long-term objective of our work is to assess the feasibility of encapsulating R-PE for use as the critical component of a time-temperature integrator (TTI) for ascertaining the degr...

journal_title：Biotechnology and bioengineering

authors： Vaidya S,Orta-Ramirez A,Smith DM,Ofoli RY

更新日期：2003-08-20 00:00:00

abstract：:Both k(L)a and k(L) measurements were carried out by an unsteady state technique at impeller speeds ranging from 1.6 to 5.8 s(-1) in a mechanically agitated animal cell culture vessel of working volume 1.5 L. Checks were made that the time constant of the oxygen electrode was negligible compared to the time for aerati...

journal_title：Biotechnology and bioengineering

authors： Lavery M,Nienow AW

更新日期：1987-08-20 00:00:00

abstract：:Clostridium difficile has emerged as a major cause of infectious diarrhea in hospitalized patients, with increasing mortality rate and annual healthcare costs exceeding $3 billion. Since C. difficile infections are associated with the use of antibiotics, there is an urgent need to develop treatments that can inactivat...

journal_title：Biotechnology and bioengineering

authors： Mehta KK,Paskaleva EE,Wu X,Grover N,Mundra RV,Chen K,Zhang Y,Yang Z,Feng H,Dordick JS,Kane RS

更新日期：2016-12-01 00:00:00

abstract：:The sufficient provision of oxygen is mandatory for enzymatic oxidations in aqueous solution, however, in process optimization this still is a bottleneck that cannot be overcome with the established methods of macrobubble aeration. Providing higher mass transfer performance through microbubble aerators, inefficient ae...

journal_title：Biotechnology and bioengineering

authors： Thomas B,Ohde D,Matthes S,Engelmann C,Bubenheim P,Terasaka K,Schlüter M,Liese A

更新日期：2021-01-01 00:00:00

abstract：:Phosphorus-31 nuclear magnetic resonance ((31)P NMR) was used to compare the anaerobic metabolism of glucose by suspended and gel-entrapped Saccharomyces bayanus cells. The fermentation of glucose was carried out in a reaction system with continuous circulation through the NMR sample tube. The intracellular pH and the...

journal_title：Biotechnology and bioengineering

authors： Taipa MA,Cabral JM,Santos H

更新日期：1993-03-15 00:00:00

abstract：:The amount of ATP produced during n-alkane, fatty acid, or acetate metabolism in Candida tropicalis has been established from the P/O ratios measured on isolated mitochondria, yield on substrate and carbon balance. For these three kinds of substrates YATP value has been found to be close to 4, although Ysub on acetate...

journal_title：Biotechnology and bioengineering

authors： Gallo M,Azoulay E

更新日期：1975-12-01 00:00:00

abstract：:Steady-state simulations using our previously developed structured kinetic model of antibody synthesis and secretion by hybridoma cells are used here in conjunction with factorial design analysis to identify intracellular parameters important in determining the specific antibody secretion rate and predict the dependen...

journal_title：Biotechnology and bioengineering

authors： Bibila TA,Flickinger MC

更新日期：1992-02-05 00:00:00

abstract：:Monolith reactors combine good mass transfer characteristics with low-pressure drop, the principle factors affecting the cost effectiveness of industrial processes. Recently, these specific features of the monolith reactors have drawn the attention toward the application of the monolith reactor in multiphase reaction ...

journal_title：Biotechnology and bioengineering

authors： Ebrahimi S,Kleerebezem R,Kreutzer MT,Kapteijn F,Moulijn JA,Heijnen JJ,van Loosdrecht MC

更新日期：2006-02-05 00:00:00

abstract：:Biofiltration of air polluted by volatile organic compounds is now recognized by the industrial and research communities as an effective and viable alternative to standard environmental technologies. Whereas many studies have focused on solid/liquid/gas biofilters, there have been fewer reports on waste air treatment ...

journal_title：Biotechnology and bioengineering

authors： Erable B,Goubet I,Lamare S,Legoy MD,Maugard T

更新日期：2004-04-05 00:00:00

abstract：:The hollow fiber enzyme reactor with pulsation developed by Kim and Chang (1983) was operated in a differential mode by recycling a substrate solution, in order to assess the efficiency of ultrafiltration swing. The rates of lactose conversion by beta-galactosidase contained in the shell side of the reactor were measu...

journal_title：Biotechnology and bioengineering

authors： Park TH,Kim IH,Chang HN

更新日期：1985-08-01 00:00:00

abstract：:Regenerative medicine has emerged as an important discipline that aims to repair injury or replace damaged tissues or organs by introducing living cells or functioning tissues. Successful regenerative medicine strategies will likely depend upon a simultaneous optimization strategy for the design of biomaterials, cell-...

journal_title：Biotechnology and bioengineering

authors： Tang Q,Piard C,Lin J,Nan K,Guo T,Caccamese J,Fisher J,Chen Y

更新日期：2018-01-01 00:00:00

abstract：:Microcrystalline cellulose (10 g/L Avicel) was hydrolysed by two major cellulases, cellobiohydrolase I (CBH I) and endoglucanase II (EG II), of Trichoderma reesei. Two types of experiments were performed, and in both cases the enzymes were added alone and together, in equimolar mixtures. In time course studies the rea...

journal_title：Biotechnology and bioengineering

authors： Medve J,Karlsson J,Lee D,Tjerneld F

更新日期：1998-09-05 00:00:00

abstract：:The Model presented in this work demonstrates the combination of cell-cycle model with a model describing the growth and conversion kinetics of hybridoma cells in a steady-state continuous culture. The cell-cycle model is based upon a population balance model as described by Cazzador et al. and assumes the existence o...

journal_title：Biotechnology and bioengineering

authors： Martens DE,Sipkema EM,de Gooijer CD,Beuvery EC,Tramper J

更新日期：1995-10-05 00:00:00

abstract：:Pre-filtration using ion exchange membrane adsorbers can improve parvovirus filter throughput of monoclonal antibodies (mAbs). The membranes work by binding trace foulants, and although some antibody product also binds, yields > or =99% are easily achieved by overloading. Results show that foulant adsorption is depend...

journal_title：Biotechnology and bioengineering

authors： Brown A,Bechtel C,Bill J,Liu H,Liu J,McDonald D,Pai S,Radhamohan A,Renslow R,Thayer B,Yohe S,Dowd C

更新日期：2010-07-01 00:00:00

abstract：:We studied the resolution of racemic glycidol through esterification with butyric acid catalyzed by porcine pancreatic lipase in organic media. A screening of seven solvents (log P values between 0.49 and 3.0, P being the n-octanol-water partition coefficient of the solvent) showed that neither log P nor the logarithm...

journal_title：Biotechnology and bioengineering

authors： Martins JF,Da Ponte MN,Barreiros S

更新日期：1993-08-05 00:00:00

abstract：:A mathematical model is formulated to describe trends in biomass and penicillin formation as well as substrate consumption for fed-batch cultivations. The biomass is structured into three morphological compartments, and glucose and corn steep liquor are considered as substrates for growth. Penicillin formation is assu...

journal_title：Biotechnology and bioengineering

authors： Zangirolami TC,Johansen CL,Nielsen J,Jørgensen SB

更新日期：1997-12-20 00:00:00

abstract：:Two instruments were developed to mechanize the handling of anaerobic microorganisms for microbial mutant isolation. The instruments automatically dispense liquid or agar medium to large and small 96-well platesand petridishes. Protocols were developed for inoculating different microorganisms, and calibration curves o...

journal_title：Biotechnology and bioengineering

authors： Stieglitz B,Defelice CP

更新日期：1986-09-01 00:00:00

abstract：:Continuous propionic acid fermentations of lactate by Propionibacterium acidipropionici were studied in spiral wound fibrous bed bioreactors. Cells were imobilized by natural attachment to fiber surfaces and entrapment in the void volume within the fibrous matrix. A high cell density of approximately 37 g/L was attain...

journal_title：Biotechnology and bioengineering

authors： Lewis VP,Yang ST

更新日期：1992-08-05 00:00:00

abstract：:A model is proposed that accounts for the decreases in yield which occur in chemostat cultures of mesophilic yeasts at superoptimal growth temperatures. Two yield depressing effects were identified, one due to increased maintenance requirements by the viable fraction of the population, the other due to energy substrat...

journal_title：Biotechnology and bioengineering

authors： Van Uden N,Madeira-Lopes A

更新日期：1976-06-01 00:00:00

abstract：:Oil residues arising from the Christos-Bitas spillage were found to contain 28% of oil extractable by carbon tetrachloride; the remainder consisted of water and undefined solids. Christos-Bitas mousse was added to 1.18 m(3) liquor inoculated with oil-contaminated marine mud, and aerated with a 1.5-hp vortex pump and v...

journal_title：Biotechnology and bioengineering

authors： Berwick PG

更新日期：1985-01-01 00:00:00

abstract：:Chemotaxis has been shown to be beneficial for the migration of soil-inhabiting bacteria towards industrial chemical pollutants, which they degrade. Many studies have demonstrated the importance of this microbial property under various circumstances; however, few quantitative analyses have been undertaken to measure t...

journal_title：Biotechnology and bioengineering

authors： Wang X,Atencia J,Ford RM

更新日期：2015-05-01 00:00:00

abstract：:Animal cell cultivation in vitro has been studied for more than 40 years. However, the culture medium composition has not been designed on the basis of the stoichiometric nutritional demands for animal cell growth. In this article, a model was developed to study the stoichiometric demands for nutrients (including gluc...

journal_title：Biotechnology and bioengineering

authors： Xie L,Wang DI

更新日期：1994-05-01 00:00:00