Biotransformation of D-methionine into L-methionine in the cascade of four enzymes.

abstract：:In this study we report the isolation of four denitrifying bacteria from a batch reactor, where the progress of hydrogenotrophic denitrification was examined. Only three of the strains had the ability to use hydrogen as electron donor. In the present work, kinetic batch experiments were carried out in order to study t...

journal_title：Biotechnology and bioengineering

authors： Vasiliadou IA,Siozios S,Papadas IT,Bourtzis K,Pavlou S,Vayenas DV

更新日期：2006-10-20 00:00:00

abstract：:This article studies the operation of a new process for the production of biopolymers (polyhydroxyalkanoates, PHAs) at different applied organic load rates (OLRs). The process is based on the aerobic enrichment of activated sludge to obtain mixed cultures able to store PHAs at high rates and yields. A mixture of aceti...

journal_title：Biotechnology and bioengineering

authors： Dionisi D,Majone M,Vallini G,Di Gregorio S,Beccari M

更新日期：2006-01-05 00:00:00

abstract：:The discovery of denitrifying anaerobic methane oxidation (DAMO) processes enables the complete nitrogen removal from wastewater by utilizing the methane produced on site from anaerobic digesters. This model-based study investigated the mechanisms and operational window for efficient nitrogen removal by coupling nitri...

journal_title：Biotechnology and bioengineering

authors： Chen X,Guo J,Xie GJ,Yuan Z,Ni BJ

更新日期：2016-05-01 00:00:00

abstract：:Development of bioengineering using such biomaterials as plant tissues or genetically produced microorganisms makes it important to accurately estimate the prevailing shear rate in bioreactors because they are generally sensitive to shear. Various authors have discussed ways to estimate the average shear rate in a bub...

journal_title：Biotechnology and bioengineering

authors： Nishikawa M

更新日期：1991-03-25 00:00:00

abstract：:The high commercial values of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids have driven a strain-improvement program, aimed at increasing the content of those fatty acids in the microalga Pavlova lutheri (SMBA 60) as parent strain. After a round of mutation using UV-light as mutagenic agent, an isolate strain...

journal_title：Biotechnology and bioengineering

authors： Meireles LA,Guedes AC,Malcata FX

更新日期：2003-01-05 00:00:00

abstract：:Two attractive features of ELISA are the specificity of antibody-antigen recognition and the sensitivity achieved by enzymatic amplification. This report describes the development of a non-enzymatic molecular recognition platform adaptable to point-of-care clinical settings and field detection of biohazardous material...

journal_title：Biotechnology and bioengineering

authors： Stone GP,Mernaugh R,Haselton FR

更新日期：2005-09-20 00:00:00

abstract：:A novel reaction system was developed to maximize the catalytic efficiency of chloroperoxidase (CPO, from Caldariomyces fumago) toward the oxidation of hydrocarbons. The reaction system consisted of an organic/aqueous emulsion comprising pure substrate and aqueous buffer supplemented with the surfactant dioctyl sulfos...

journal_title：Biotechnology and bioengineering

authors： Park JB,Clark DS

更新日期：2006-05-05 00:00:00

abstract：:A new method for solid-state ethanol fermentation (the SSEF system) was experimented on for the ethanol production from solid starchy materials, where a packedbed-type fermentor was used. Both cultivation of Aspergillus saitoi and enrichment of a saccharifying enzyme were effective for hydrolysis of the starch. Ethano...

journal_title：Biotechnology and bioengineering

authors： Sato K,Nakamura K,Sato S

更新日期：1985-09-01 00:00:00

abstract：:The kinetics of the cellulase-catalyzed conversion of soluble cellulose into glucose have been studied over a range of substrate concentrations and temperatures, and at pH values ranging from 4.75 to 7.0. Lineweaver-Burk plots were linear and led to V = 6.2muM/s and K(m) = 13.1 mM at pH 5.8 and 25.0 degrees C. The pK ...

journal_title：Biotechnology and bioengineering

authors： Banerjee DK,Laidler KJ

更新日期：1983-10-01 00:00:00

abstract：:A whole cell-based optical sensing system for copper was developed based on Saccharomyces cerevisiae cells harboring plasmid pYEX-GFPuv. The basis of this system was the ability of the transcriptional activator protein Ace1 present in S. cerevisiae to control the expression of the reporter protein, GFPuv. When copper ...

journal_title：Biotechnology and bioengineering

authors： Shetty RS,Deo SK,Liu Y,Daunert S

更新日期：2004-12-05 00:00:00

abstract：:A model for cometabolism is verified experimentally for a defined methanotrophic mixed culture. The model includes the effects of cell growth, endogenous cell decay, product toxicity, and competitive inhibition with the assumption that cometabolic transformation rates are enhanced by reducing power obtained from oxida...

journal_title：Biotechnology and bioengineering

authors： Chang WK,Criddle CS

更新日期：1997-12-05 00:00:00

abstract：:The treatment of meta- and para-nitrobenzoic acid in an industrial wastestream by Comamonas sp. JS46 and Comamonas sp. JS47 is investigated. The most important feature of the wastestream is the constantly changing concentration ratio of the two isomers. The most extreme occurrence is considered here: the complete chan...

journal_title：Biotechnology and bioengineering

authors： Goodall JL,Thomas SM,Spain JC,Peretti SW

更新日期：1998-07-05 00:00:00

abstract：:Understanding the regulatory networks which control specific macrophage phenotypes is essential in identifying novel targets to correct macrophage mediated clinical disorders, often accompanied by inflammatory events. Since mesenchymal stromal cells (MSCs) have been shown to play key roles in regulating immune functio...

journal_title：Biotechnology and bioengineering

authors： Barminko JA,Nativ NI,Schloss R,Yarmush ML

更新日期：2014-11-01 00:00:00

abstract：:Glycerol is considered as an ideal feedstock for producing bioplastics via bacterial fermentation due to its ubiquity, low price, and high degree of reduction substrate. In this work, we study the yield and cause of limitation in poly(3-hydroxybutyrate) (PHB) production from glycerol. Compared to glucose-based PHB pro...

journal_title：Biotechnology and bioengineering

authors： Tanadchangsaeng N,Yu J

更新日期：2012-11-01 00:00:00

abstract：:The sensitivity of a conceptual model of a foam emulsion bioreactor (FEBR) used for the control of toluene vapors in air was examined. Model parametric sensitivity studies showed which parameters affect the removal of toluene (as model pollutant) in the FEBR the most significantly, and enabled definition of the limits...

journal_title：Biotechnology and bioengineering

authors： Kan E,Deshusses MA

更新日期：2009-02-15 00:00:00

abstract：:Nanotechnology is becoming increasingly important for products used in our daily lives, such as the masses of titanium dioxide nanoparticle agglomerates (TiO(2) NPs) used in the pharmaceutical industry, for cosmetic products, or for pigments. Meanwhile, a serious lack of detailed information concerning the interaction...

journal_title：Biotechnology and bioengineering

authors： Chen P,Kanehira K,Sonezaki S,Taniguchi A

更新日期：2012-12-01 00:00:00

abstract：:The success of in situ bioremediation is often limited by the inability to bring bacteria in contact with the pollutant, which they will degrade. A bench-scale model aquifer was used to evaluate the impact of chemotaxis on the migration of bacteria toward the source of a chemical pollutant. The model was packed with s...

journal_title：Biotechnology and bioengineering

authors： Strobel KL,McGowan S,Bauer RD,Griebler C,Liu J,Ford RM

更新日期：2011-09-01 00:00:00

abstract：:Pretreatment of sugar cane bagasse is essential for a simultaneous saccharification and fermentation (SSF) process which uses recombinant Klebsiella oxytoca strain P2 and Genencor Spezyme CE. Strain P2 has been genetically engineered to express Zymomonas mobilis genes encoding the ethanol pathway and retains the nativ...

journal_title：Biotechnology and bioengineering

authors： Doran JB,Aldrich HC,Ingram LO

更新日期：1994-06-20 00:00:00

abstract：:Buffered aqueous two-phase systems are effective extraction systems for separating amphoteric hydrocarbons like, for example, polypeptides from aqueous phases. The design and basic engineering of such processes requires the knowledge of the liquid-liquid equilibrium. The study presented here aims to contribute to the ...

journal_title：Biotechnology and bioengineering

authors： Grossmann C,Tintinger R,Zhu J,Maurer G

更新日期：1998-12-20 00:00:00

abstract：:We show the design features of a membrane bioreactor based on pulsatile flow across dimpled membranes. Results show an enhanced mass transfer of air of at least five-fold magnitude as compared with flat membranes. An increased working volume form 20 mL to 120 mL reduced the k(L)A at a given Reynolds number because of ...

journal_title：Biotechnology and bioengineering

authors： Beeton S,Millward HR,Bellhouse BJ,Nicholson AM,Jenkins N,Knowles CJ

更新日期：1991-12-05 00:00:00

abstract：:Cross-linked polyurethane (PU) was prepared for entrapping thermolysin. Using the immobilized thermolysin (IT), Z-L-aspartic acid (ZA) was reacted with -Lphenylalanine methyl ester (L-PM) in water-saturated ethyl acetate to give only alpha-Z-L-aspartylL-phenylalanine methyl ester (alpha-ZAPM). Ninety-four percent conv...

journal_title：Biotechnology and bioengineering

authors： Yang CP,Su CS

更新日期：1988-08-20 00:00:00

abstract：:Knowledge of the aqueous phase methane concentration is critical to understanding and controlling process kinetics in methanotrophic bioreactors. Unfortunately since no dissolved methane probe is commercially available, this data must be obtained off-line by the time-consuming gas-liquid partition method. In this stud...

journal_title：Biotechnology and bioengineering

authors： Yu Y,Ramsay JA,Ramsay BA

更新日期：2006-12-05 00:00:00

abstract：:Hairy roots grow quickly, reach high densities, and can produce significant amounts of secondary metabolites, yet their scale-up to bioreactors remains challenging. Artemisia annua produces a rich array of terpenoids, including the sesquiterpene, artemisinin, and transformed roots of this species provide a good model ...

journal_title：Biotechnology and bioengineering

authors： Souret FF,Kim Y,Wyslouzil BE,Wobbe KK,Weathers PJ

更新日期：2003-09-20 00:00:00

abstract：:A proteinase isolated from Thermus RT41a was immobilized to controlled pore glass beads and was used in the free and immobilized forms for peptide synthesis. The observed maximum yield was the same in both cases. a number of dipeptides were produced from amino acid esters and amides. The best acyl components, from tho...

journal_title：Biotechnology and bioengineering

authors： Wilson SA,Daniel RM,Peek K

更新日期：1994-07-01 00:00:00

abstract：:The dissolved oxygen (DO) is an important variable in aerobic fermentations and affects the cell growth and product formation. Dissolved oxygen control is difficult in batch fermentations because of the time-varying conditions, time delays, and the probe dynamics. Modeling of the various patterns of biological activit...

journal_title：Biotechnology and bioengineering

authors： Sargantanis IG,Karim MN

更新日期：1998-10-05 00:00:00

abstract：:An in vitro toxicity testing system that combines perfusion of a membrane-supported tissue model with measurement of trans-epithelial electrical resistance (TER) is described. The system is fully automated and allows for continuous TER measurements during exposure to a test agent. The utility of this system is demonst...

journal_title：Biotechnology and bioengineering

authors： Pasternak AS,Miller WM

更新日期：1996-06-05 00:00:00

abstract：:Although microalgae are considered as a promising feedstock for biofuels, the energy efficiency of the production process needs to be significantly improved. Due to their small size and low concentration in the culture medium, cost-efficient harvesting of microalgae is a major challenge. In this study, the use of elec...

journal_title：Biotechnology and bioengineering

authors： Vandamme D,Pontes SC,Goiris K,Foubert I,Pinoy LJ,Muylaert K

更新日期：2011-10-01 00:00:00

abstract：:The direct microbial conversion (DMC) process for the production of ethanol from lignocellulosic biomass is limited by low volumetric ethanol production rates due to the low cell densities of Clostridium thermosaccharolyticum which is a key organism for ethanol production in this process. Hence, this study focuses on ...

journal_title：Biotechnology and bioengineering

authors： Mistry FR,Cooney CL

更新日期：1989-12-05 00:00:00

abstract：:Kluyveromyces marxianus is a promising nonconventional yeast for biobased chemical production due to its rapid growth rate, high TCA cycle flux, and tolerance to low pH and high temperature. Unlike Saccharomyces cerevisiae, K. marxianus grows on low-cost substrates to cell densities that equal or surpass densities in ...

journal_title：Biotechnology and bioengineering

authors： McTaggart TL,Bever D,Bassett S,Da Silva NA

更新日期：2019-07-01 00:00:00

abstract：:The production of ethanol from carob pod extract by free and immobilized Saccharomyces cerevisiae cells in batch and fed-batch culture was investigated. Fed-batch culture proved to be a better fermentation system for the production of ethanol than batch culture. In fed-batch culture, both free and immobilized S. cerev...

journal_title：Biotechnology and bioengineering

authors： Roukas T

更新日期：1994-02-05 00:00:00