Abstract:
:This article describes a method for the chemical immobilization of concanavalin A (Con A) on the inside wall of a single hollow cellulose fiber for use in glucose affinity sensor. Periodate oxidation of cellulose fiber followed by a spacer for Con A attachment was deemed to be the most optimal procedure for achieving the highest sensitivity of the sensor without compromising its physical integrity. The effects of variables like the duration of periodate oxidation and its concentration and pH of the spacer coupling step and its duration have been examined. The mechanical strength of the hollow fiber as well as its permeability to the analyte (glucose) have been evaluated prior to and after Con A coupling process. It has been demonstrated that Con A bound hollow fiber prepared according to the procedure outlined here can be successfully used to construct glucose affinity sensor for operation in the physiological range of glucose concentrations.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Srinivasan KR,Mansouri S,Schultz JSdoi
10.1002/bit.260280213subject
Has Abstractpub_date
1986-02-01 00:00:00pages
233-9issue
2eissn
0006-3592issn
1097-0290journal_volume
28pub_type
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