Application of temperature-sensitive mutants for single-cell protein production.

abstract：:Sugarcane bagasse is an abundant and sustainable resource, generated as a by-product of sugarcane milling. The cellulosic material within bagasse can be broken down into glucose molecules and fermented to produce ethanol, making it a promising feedstock for biofuel production. Mild acid pretreatment hydrolyses the hem...

journal_title：Biotechnology and bioengineering

authors： Greenwood AA,Farrell TW,O'Hara IM

更新日期：2013-12-01 00:00:00

abstract：:A generic "system on a plate" modular multicompartmental bioreactor array which enables microwell protocols to be transferred directly to the bioreactor modules, without redesign of cell culture experiments or protocols is described. The modular bioreactors are simple to assemble and use and can be easily compared wit...

journal_title：Biotechnology and bioengineering

authors： Mazzei D,Guzzardi MA,Giusti S,Ahluwalia A

更新日期：2010-05-01 00:00:00

abstract：:In this article, a kinetic model is developed and presented for biological nutrient removal (BNR) activated sludge (BNRAS) systems in general, but for external nitrification (EN) BNRAS (ENBNRAS) systems in particular. The model is based on the UCTPHO model, but includes some significant modifications, such as anoxic P...

journal_title：Biotechnology and bioengineering

authors： Hu ZR,Wentzel MC,Ekama GA

更新日期：2007-12-15 00:00:00

abstract：:Acid phosphatase thermal deactivation follows a complex path: an initial decay toward an equilibrium distribution of at least two intermediate structures, mutually at the equilibrium, followed by a final breakdown toward a completely inactive enzyme configuration. The results obtained in the presence of sorbitol have ...

journal_title：Biotechnology and bioengineering

authors： Gianfreda L,Toscano G,Pirozzi D,Greco G Jr

更新日期：1991-12-05 00:00:00

abstract：:The transesterification reaction of N-acetyl-L-phenylalanine ethyl ester with 1-propanol catalyzed by alpha-chymotrypsin was examined in the ionic liquids 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim][PF(6)]) and 1-octyl-3-methylimidazolium hexafluorophosphate ([omim][PF(6)]), and in combination with supercr...

journal_title：Biotechnology and bioengineering

authors： Laszlo JA,Compton DL

更新日期：2001-10-20 00:00:00

abstract：:In this study, omics-based analysis tools were used to explore the effect of glucose starvation and culture duration on monoclonal antibody (mAb) production in fed-batch CHO cell culture to gain better insight into how these parameters can be controlled to ensure optimal mAb productivity and quality. Titer and N-glyco...

journal_title：Biotechnology and bioengineering

authors： Fan Y,Jimenez Del Val I,Müller C,Lund AM,Sen JW,Rasmussen SK,Kontoravdi C,Baycin-Hizal D,Betenbaugh MJ,Weilguny D,Andersen MR

更新日期：2015-10-01 00:00:00

abstract：:Medium-chain-length-polyhydroxyalkanoic acids (MCL-PHAs) formed in Pseudomonas spp. have a rather broad distribution of monomer-units whose precursors are supplied via beta-oxidation degradation of MCL fatty acids fed as the carbon source and/or via PhaG enzyme catalyzing the acyl-group transfer from 3-hydroxyacyl-ACP...

journal_title：Biotechnology and bioengineering

authors： Choi MH,Xu J,Rho JK,Shim JH,Yoon SC

更新日期：2009-03-01 00:00:00

abstract：:The influence of five different N-terminal protecting groups (For, Ac, Boc, Z, and Fmoc) and reaction conditions (temperature and dimethylformamide content) on the alpha-chymotrypsin-catalyzed synthesis of the dipeptide derivative X-Phe-Leu-NH(2) was studied. Groups such as For, Ac, Boc, and Z always rendered good pep...

journal_title：Biotechnology and bioengineering

authors： Calvet S,Clapés P,Vigo JP,Xaus N,Jorba X,Mas RM,Torres JL,Valencia G,Serralheiro ML,Cabral JM,Empis JM

更新日期：1992-03-05 00:00:00

abstract：:A method is described to estimate the microbial biomass of a sample, to enumerate the cells, and to distinguish the portion of metabolically active cells in the population by measuring the binding of phenyldicarbaundecaborane (PCB(-)) to the cells. This method can also be used for the analysis of a complex population ...

journal_title：Biotechnology and bioengineering

authors： Daugelavicius R,Bakiene E,Berzinskiene J,Bamford DH

更新日期：2000-01-01 00:00:00

abstract：:In order to predict the potential benefit associated with mixing devices designed to introduce periodic light modulations in dense cultures of microalgae, it is necessary to develop a quantitative understanding of the relationship between the frequency of the modulations and the resulting photosynthetic efficiency enh...

journal_title：Biotechnology and bioengineering

authors： Terry KL

更新日期：1986-07-01 00:00:00

abstract：:The purpose of this study was to understand bacteria profile modification and its applications in subsurface biological operations such as biobarrier formation, in situ bioremediation, and microbial-enhanced oil recovery. Biomass accumulation and evolution in porous media were investigated both experimentally and theo...

journal_title：Biotechnology and bioengineering

authors： Kim DS,Fogler HS

更新日期：2000-07-05 00:00:00

abstract：:Numerous biotechnological production processes are based on the submerse cultivation of filamentous fungi. Process design, however, is often hampered by the complex growth pattern of these organisms. In the morphologic development of coagulating filamentous fungi, like Aspergillus niger, conidial aggregation is the fi...

journal_title：Biotechnology and bioengineering

authors： Lin PJ,Grimm LH,Wulkow M,Hempel DC,Krull R

更新日期：2008-02-01 00:00:00

abstract：:A systematic, kinetic study and comparison of the leaching of mixed metal sulfides by galvanic conversion and in the presence of bacteria has been carried out for the first time using both powder (-100 to -400 mesh) and larger (bulk) specimen systems. The rates of dissolution of copper from chalcopyrite and zinc from ...

journal_title：Biotechnology and bioengineering

authors： Mehta AP,Murr LE

更新日期：1982-04-01 00:00:00

abstract：:Aspergillus terreus cadA, encoding cis-aconitate decarboxylase, is an essential gene for itaconic acid (IA) biosynthesis, but it is primarily expressed as insoluble aggregates in most industrial hosts. This has been a hurdle for the development of recombinant strategies for IA production. Here, we created a library of...

journal_title：Biotechnology and bioengineering

authors： Jeon HG,Cheong DE,Han Y,Song JJ,Choi JH

更新日期：2016-07-01 00:00:00

abstract：:An immobilized enzyme reactor has been developed for the degradation of bilirubin as a potential treatment for neonatal jaundice. It utilizes the enzyme bilirubin oxidase from Myrothecium verrucaria, which in the presence of molecular oxygen converts bilirubin to biliverdin and other products that are much less toxic ...

journal_title：Biotechnology and bioengineering

authors： Sung C,Lavin A,Klibanov AM,Langer R

更新日期：1986-10-01 00:00:00

abstract：:The kinetic properties of acetate-degrading methanogenic granular sludge of different mean diameters were assessed at different up-flow velocities (V(up)). Using this approach, the influence of internal and external mass transfer could be estimated. First, the apparent Monod constant (K(S)) for each data set was calcu...

journal_title：Biotechnology and bioengineering

authors： Gonzalez-Gil G,Seghezzo L,Lettinga G,Kleerebezem R

更新日期：2001-04-20 00:00:00

abstract：:A method was developed for obtaining high signal-to-noise 13C NMR spectra of intracellular compounds in metabolically active cultured cells. The method allows TCA cycle labeling kinetics to be determined in real time without significant oxygen transport limitations. Cells were immobilized on the surface of nonporous m...

journal_title：Biotechnology and bioengineering

authors： Mancuso A,Beardsley NJ,Wehrli S,Pickup S,Matschinsky FM,Glickson JD

更新日期：2004-09-30 00:00:00

abstract：:Aryl-alcohol oxidase (AAO) plays a fundamental role in the fungal ligninolytic secretome, acting as a supplier of H2 O2 . Despite its highly selective mechanism of action, the presence of this flavooxidase in different biotechnological settings has hitherto been hampered by the lack of appropriate heterologous express...

journal_title：Biotechnology and bioengineering

authors： Viña-Gonzalez J,Elbl K,Ponte X,Valero F,Alcalde M

更新日期：2018-07-01 00:00:00

abstract：:Rapid advances in intensifying upstream processes for biologics production have left downstream processing as a bottleneck in the manufacturing scheme. Biomanufacturers are pursuing continuous downstream process development to increase efficiency and flexibility, reduce footprint and cost of goods, and improve product...

journal_title：Biotechnology and bioengineering

authors： Somasundaram B,Pleitt K,Shave E,Baker K,Lua LHL

更新日期：2018-12-01 00:00:00

abstract：:In this study, an analysis of the transition between the inactive ("closed") and active ("open") conformations of Candida rugosa lipase in solution is performed using irreversible enzyme inhibitors, cyclic saligenin phosphates. It is shown that >90% inhibition of the enzyme activity toward water-soluble substrates (es...

journal_title：Biotechnology and bioengineering

authors： Turner NA,Needs EC,Khan JA,Vulfson EN

更新日期：2001-01-05 00:00:00

abstract：:Intracellular events that take place during influenza virus replication in animal cells are well understood qualitatively. However, to better understand the complex interaction of the virus with its host cell and to quantitatively analyze the use of cellular resources for virion formation or the overall dynamic for th...

journal_title：Biotechnology and bioengineering

authors： Sidorenko Y,Reichl U

更新日期：2004-10-05 00:00:00

abstract：:The effects of low intensity pulsed ultrasound (LIPUS) on proliferation and chondrogenic differentiation of human mesenchymal stem cells (hMSCs) seeded on 3D printed poly-(ethylene glycol)-diacrylate (PEG-DA) scaffolds with varying pore geometries (square and hexagonal channels) were investigated. The scaffold with sq...

journal_title：Biotechnology and bioengineering

authors： Aliabouzar M,Lee SJ,Zhou X,Zhang GL,Sarkar K

更新日期：2018-02-01 00:00:00

abstract：:The G-H loop of foot-and-mouth disease virus is a disordered protrusion of the VP1 protein exposed on the virion surface. This short stretch includes an arginine-glycine-aspartic acid tripeptide, a recognized integrin-binding motif, which is responsible for cell attachment and infection. Eight copies of a peptide repr...

journal_title：Biotechnology and bioengineering

authors： Villaverde A,Feliu JX,Arís A,Harbottle RP,Benito A,Coutelle C

更新日期：1998-08-05 00:00:00

abstract：:The placement of the reference electrode (RE) in various bioelectrochemical systems is often varied to accommodate different reactor configurations. While the effect of the RE placement is well understood from a strictly electrochemistry perspective, there are impacts on exoelectrogenic biofilms in engineered systems ...

journal_title：Biotechnology and bioengineering

authors： Zhang F,Liu J,Ivanov I,Hatzell MC,Yang W,Ahn Y,Logan BE

更新日期：2014-10-01 00:00:00

abstract：:Glycolytic flux is increased and acetate production is reduced in Escherichia coli by the expression of heterologous NADH oxidase (NOX) from Streptococcus pneumoniae coupled with the deletion of the arcA gene, which encodes the ArcA regulatory protein. In this study, we examined the overproduction of a model recombina...

journal_title：Biotechnology and bioengineering

authors： Vemuri GN,Eiteman MA,Altman E

更新日期：2006-06-20 00:00:00

abstract：:A new type of liquid emulsion membrane containing reversed micelles for protein extraction is introduced. A three-step extraction mechanism is proposed including solubilization, transportation, and release of the protein. The surfactants Span80 and sodium di(2-ethylhexyl)sulfosuccinate (AOT) are used to stabilize the ...

journal_title：Biotechnology and bioengineering

authors： Stobbe H,Yunguang X,Zihao W,Jufu F

更新日期：1997-02-05 00:00:00

abstract：:As photoautotrophic prokaryotes, cyanobacteria are promising platforms for producing value-added bioproducts. However, few regulatory genetic parts and devices (e.g., inducible promoters and regulatory circuits) have been developed for these potential hosts. Furthermore, the devices that have been created respond only...

journal_title：Biotechnology and bioengineering

authors： Immethun CM,Ng KM,DeLorenzo DM,Waldron-Feinstein B,Lee YC,Moon TS

更新日期：2016-02-01 00:00:00

abstract：:An algorithm for operating a fed-batch fermentor at an optimum specific fermentation rate is proposed. It does not require on-line measurement of nutrient concentration in the culture medium. An on-line estimate of the specific fermentation rate is sufficient for implementation of this scheme. The algorithm is model i...

journal_title：Biotechnology and bioengineering

authors： Agrawal P,Koshy G,Ramseier M

更新日期：1989-01-05 00:00:00

abstract：:Saccharomyces cerevisiae is a widely used host organism for the production of heterologous proteins, often cultivated in glucose-based fed-batch processes. This production system however has many factors limiting the productivity, mainly towards the end of the fermentation. For the optimised production of a Camelid an...

journal_title：Biotechnology and bioengineering

authors： van de Laar T,Visser C,Holster M,López CG,Kreuning D,Sierkstra L,Lindner N,Verrips T

更新日期：2007-02-15 00:00:00

abstract：:A set of eight closely related plasmid constructs carrying CI857-controlled recombinant genes has been used as a model to study plasmid stability in Escherichia coli, in the absence of antibiotic selection. Plasmid loss rates and relative interdivision times of plasmid-bearing cells and plasmid-free cells have been an...

journal_title：Biotechnology and bioengineering

authors： Corchero JL,Villaverde A

更新日期：1998-06-20 00:00:00