Laboratory-scale production of acetoin plus diacetyl by Enterobacter cloacae ATCC 27613.

abstract：:The problems of engineering increased flux in metabolic pathways are analyzed in terms of the understanding provided by metabolic control analysis. Over-expression of a single enzyme is unlikely to be effective unless it is known to have a high flux control coefficient, which can be used as an approximate predictive t...

journal_title：Biotechnology and bioengineering

authors： Fell DA

更新日期：1998-04-05 00:00:00

abstract：:Fast and efficient production of recombinant proteins for structural and functional studies is a crucial issue for research and for industry. To this end, we have developed an efficient system to generate in less than 2 months, starting from the cDNA, pools of CHO cells stably expressing high-level of recombinant prot...

journal_title：Biotechnology and bioengineering

authors： Gaillet B,Gilbert R,Broussau S,Pilotte A,Malenfant F,Mullick A,Garnier A,Massie B

更新日期：2010-06-01 00:00:00

abstract：:Dehydrogenase activity assay of activated sludge using the redox dye 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride (INT) was investigated. INT-dehydrogenase activity (INT-DHA) was directly proportional to INT dosage and inversely proportional to bio-mass concentration over limited ranges. INT dosages...

journal_title：Biotechnology and bioengineering

authors： Lopez JM,Koopman B,Bitton G

更新日期：1986-07-01 00:00:00

abstract：:The dissolved oxygen (DO) is an important variable in aerobic fermentations and affects the cell growth and product formation. Dissolved oxygen control is difficult in batch fermentations because of the time-varying conditions, time delays, and the probe dynamics. Modeling of the various patterns of biological activit...

journal_title：Biotechnology and bioengineering

authors： Sargantanis IG,Karim MN

更新日期：1998-10-05 00:00:00

abstract：:Non-destructive label-free continuous monitoring of in vitro tissue culture is an unmet demand in tissue engineering. Noting that different compositions of cartilage lead to different electrical tissue properties, we propose a new method to measure the electrical conductivity and its anisotropy during in vitro chondro...

journal_title：Biotechnology and bioengineering

authors： Oh TI,Kim C,Karki B,Son Y,Lee E,Woo EJ

更新日期：2015-02-01 00:00:00

abstract：:CTLA4 is a membrane receptor on cytotoxic T cells whose interaction with the B7 counterreceptor on B cells is important in alloantigen responses. Soluble recombinant human and murine CTLA4 were produced using either Chinese hamster ovary or NS-0 cell lines. Expression vectors were constructed containing the gene codin...

journal_title：Biotechnology and bioengineering

authors： Flesher AR,Marzowski J,Wang WC,Raff HV

更新日期：1995-06-05 00:00:00

abstract：:The sec-alcohol dehydrogenase activity of whole cells of Rhodococcus ruber DSM 44541 has been employed as an efficient biocatalytic redox system due to the use of acetone and 2-propanol at elevated concentrations for cofactor regeneration in the oxidation and reduction mode, respectively, and external addition of NADH...

journal_title：Biotechnology and bioengineering

authors： Stampfer W,Kosjek B,Kroutil W,Faber K

更新日期：2003-03-30 00:00:00

abstract：:In this article, we consider the impact on downstream process design resulting from the use of metabolically engineered yeast strains. We address the issue of how manipulation of cell wall permeability can improve the release and subsequent recovery of heterologous products produced in yeast. ...

journal_title：Biotechnology and bioengineering

authors： Ferrer P,Diers I,Asenjo JA,Andrews BA

更新日期：1998-04-20 00:00:00

abstract：:We show the design features of a membrane bioreactor based on pulsatile flow across dimpled membranes. Results show an enhanced mass transfer of air of at least five-fold magnitude as compared with flat membranes. An increased working volume form 20 mL to 120 mL reduced the k(L)A at a given Reynolds number because of ...

journal_title：Biotechnology and bioengineering

authors： Beeton S,Millward HR,Bellhouse BJ,Nicholson AM,Jenkins N,Knowles CJ

更新日期：1991-12-05 00:00:00

abstract：:The functional analysis of plant cells at the cellular and subcellular levels requires novel technologies for the directed manipulation of individual cells. In this report, we demonstrate the use of an infrared (1,064 nm) picosecond laser for the perforation of tobacco cell protoplasts. A single pulse was sufficient t...

journal_title：Biotechnology and bioengineering

authors： Schinkel H,Jacobs P,Schillberg S,Wehner M

更新日期：2008-01-01 00:00:00

abstract：:Using solid-phase organic synthesis, we have prepared a 432-member (18 x 8 x 3) sample library based on the AG 490 "tyrphostin" template. By utilizing 432 reactors each equipped with a unique radiofrequency memory ID tag, the 432 products could be obtained as discrete entities (i.e., not as mixtures) via 18 + 8 + 3, o...

journal_title：Biotechnology and bioengineering

authors： Shi S,Xiao XY,Czarnik AW

更新日期：1998-01-01 00:00:00

abstract：:Fungal morphology in many filamentous fungal fermentations leads to high broth viscosity which limits oxygen mass transfer, and often results in reduced productivity. The objective in this study was to determine if a simple, fed-batch, process strategy-pulsed addition of limiting-carbon source-could be used to reduce ...

journal_title：Biotechnology and bioengineering

authors： Bhargava S,Wenger KS,Marten MR

更新日期：2003-04-05 00:00:00

abstract：:Extraction of intracellular protein from Escherichia coli is traditionally achieved by mechanical disruption. A chemical treatment that destroys the integrity of the bacterial cell wall and could provide an alternative technique is examined in this study. Treatment with a combination of the chelating agent ethylenedia...

journal_title：Biotechnology and bioengineering

authors： Falconer RJ,O'Neill BK,Middelberg AP

更新日期：1997-03-05 00:00:00

abstract：:Pseudomonads are serious candidates for siderophore production applied to toxic metal (TM) solubilization. The bioaugmentation of contaminated soils by these TM-solubilizing bacteria combined with phytoextraction is an emerging clean-up technology. Unfortunately, siderophore synthesis may be drastically reduced by sol...

journal_title：Biotechnology and bioengineering

authors： Braud A,Jézéquel K,Léger MA,Lebeau T

更新日期：2006-08-20 00:00:00

abstract：:In this study, the effects of the electric charges and fields on the viability of airborne microorganisms were investigated. The electric charges of different magnitude and polarity were imparted on airborne microbial cells by a means of induction charging. The airborne microorganisms carrying different electric charg...

journal_title：Biotechnology and bioengineering

authors： Mainelis G,Górny RL,Reponen T,Trunov M,Grinshpun SA,Baron P,Yadav J,Willeke K

更新日期：2002-07-20 00:00:00

abstract：:Water permeability (Lp), calculated from the volume variations of cells subjected to an osmotic shock, is classically used to characterize cell membrane properties. In this work, we have shown the importance of the kind of mixing reactor used to measure the Lp parameter. A mathematical model including the mixing time ...

journal_title：Biotechnology and bioengineering

authors： de Marañón IM,Gervais P,Molin P

更新日期：1997-10-05 00:00:00

abstract：:Green fluorescent protein (GFP) was used to study the regulation of the galactose-inducible GAL1 promoter in yeast Saccharomyces cerevisiae strains. GFP was cloned into the pGAL110 vector and transformed into the yeast strains. Time course studies comparing culture fluorescence intensity and GFP concentration were con...

journal_title：Biotechnology and bioengineering

authors： Li J,Wang S,VanDusen WJ,Schultz LD,George HA,Herber WK,Chae HJ,Bentley WE,Rao G

更新日期：2000-10-20 00:00:00

abstract：:Bacterial biofilms are a major obstacle challenging the development of more effective therapies to treat implant infections. Oxygen availability to bacterial cells has been implicated in biofilm formation and planktonic cell detachment; however, there are insufficient tools available to measure oxygen concentrations w...

journal_title：Biotechnology and bioengineering

authors： Acosta MA,Velasquez M,Williams K,Ross JM,Leach JB

更新日期：2012-10-01 00:00:00

abstract：:The sensitivity of a conceptual model of a foam emulsion bioreactor (FEBR) used for the control of toluene vapors in air was examined. Model parametric sensitivity studies showed which parameters affect the removal of toluene (as model pollutant) in the FEBR the most significantly, and enabled definition of the limits...

journal_title：Biotechnology and bioengineering

authors： Kan E,Deshusses MA

更新日期：2009-02-15 00:00:00

abstract：:The development of a lentiviral system to deliver genes to specific cell types could improve the safety and the efficacy of gene delivery. Previously, we have developed an efficient method to target lentivectors to specific cells via an antibody-antigen interaction in vitro and in vivo. We report herein a targeted len...

journal_title：Biotechnology and bioengineering

authors： Froelich S,Ziegler L,Stroup K,Wang P

更新日期：2009-09-01 00:00:00

abstract：:A biosensor detecting estrogens, progestogens, and androgens in complex samples and in a single step is described. Three Arxula adeninivorans yeast strains were created, each strain producing a different recombinant human hormone receptor and a different fluorescent reporter protein. These strains were then mixed to c...

journal_title：Biotechnology and bioengineering

authors： Chamas A,Pham HTM,Jähne M,Hettwer K,Uhlig S,Simon K,Einspanier A,Baronian K,Kunze G

更新日期：2017-07-01 00:00:00

abstract：:The engineering of production cell lines to express anti-apoptotic genes has been pursued in recent years due to potential process benefits, including enhanced cell survival, increased protein expression, and improved product quality. In this study, a baby hamster kidney cell line secreting recombinant factor VIII (BH...

journal_title：Biotechnology and bioengineering

authors： Nivitchanyong T,Martinez A,Ishaque A,Murphy JE,Konstantinov K,Betenbaugh MJ,Thrift J

更新日期：2007-11-01 00:00:00

abstract：:As part of an investigation into the use of biological nitrogen fixation for fertilizer ammonia production, continuous culture studies of respiration and nitrogen fixation in the aerobic bacteria Azotobacter vinelandii under oxygen-limited conditions were conducted. Respiration and growth rates followed Monod forms wi...

journal_title：Biotechnology and bioengineering

authors： Diluccio RC,Kirwan DJ

更新日期：1984-01-01 00:00:00

abstract：:Lyophilized proteins exhibit "pH memory," i.e., their behavior in the solid form corresponds to the pH of the aqueous solution from which they were freeze dried. Herein, we directly tested whether the ionization state is "remembered" by model organic compounds containing various protein functional groups (amino, carbo...

journal_title：Biotechnology and bioengineering

authors： Costantino HR,Griebenow K,Langer R,Klibanov AM

更新日期：1997-02-05 00:00:00

abstract：:Copolymers of N-isopropylacrylamide and N-acryloyl amino acid spacers of varying chain length were synthesized. p-Aminobenzamidine (PABA) was chemically linked to the pendant carboxyl groups of these polymers to obtain thermoprecipitating affinity polymers. The inhibition constant (Ki) of these polymers for trypsin de...

journal_title：Biotechnology and bioengineering

authors： Vaidya AA,Lele BS,Kulkarni MG,Mashelkar RA

更新日期：1999-08-20 00:00:00

abstract：:The application of enantioseparation methods alone can only yield up to 50% of the desired chiral product. Thus enantioseparation becomes more attractive when accompanied by the racemization of the counter-enantiomer. Here we present first results of dynamic kinetic resolution of L-asparagine (L-Asn) via preferential ...

journal_title：Biotechnology and bioengineering

authors： Würges K,Petrusevska-Seebach K,Elsner MP,Lütz S

更新日期：2009-12-15 00:00:00

abstract：:A chimeric Fab was expressed in Chinese hamster ovary cells under the control of the CMV promoter in a two-stage production process. Cells were first grown to 90% confluence at 37 degrees C in a proliferation phase, followed by a production phase at either 37 degrees C or 28 degrees C. Medium supplemented with serum a...

journal_title：Biotechnology and bioengineering

authors： Schatz SM,Kerschbaumer RJ,Gerstenbauer G,Kral M,Dorner F,Scheiflinger F

更新日期：2003-11-20 00:00:00

abstract：:Bioartificial liver (BAL) devices have been developed to treat patients undergoing acute liver failure. One of the most important parameters to consider in designing these devices is the oxygen consumption rate of the seeded hepatocytes which are known to have oxygen consumption rates 10 times higher than most other c...

journal_title：Biotechnology and bioengineering

authors： Cho CH,Park J,Nagrath D,Tilles AW,Berthiaume F,Toner M,Yarmush ML

更新日期：2007-05-01 00:00:00

abstract：:Models for the known glucose transporters in Aspergillus niger and for simple diffusion of glucose through the hyphal membrane were prepared. The results from the models were compared with fermentation data from published studies on citric acid. It was found that the purely physical and uncontrolled process of diffusi...

journal_title：Biotechnology and bioengineering

authors： Wayman FM,Mattey M

更新日期：2000-02-20 00:00:00

abstract：:The scope in improving enzyme productivities from the cellulose fermentation process is examined in laboratory-scale fermentors. The maximum productivity (30 IU/liter hr) is attained in a continuous-culture process with cell recycle using modified medium containing 0.5% cellulose. Optimum dilution rate and recycle rat...

journal_title：Biotechnology and bioengineering

authors： Ghose TK,Sahai V

更新日期：1979-02-01 00:00:00