Abstract:
:Ultrafiltration through Carbosep M(4) mineral membrane of protein solutions of decreasing complexity (whey before and after centrifugation or clarification, beta-lactoglobulin) was studied. Mathematical models were used to explain variations in flux with time. Taking into account variations in protein retention and hydraulic resistance of the membrane during ultrafiltration, proteins and lipoproteins were found to be involved not only in the polarization layer (reversible fouling leading to a difference in the osmotic pressure), but also in irreversible fouling by adsorption. Morever, the presence of particles (e.g., inorganic precipitates) in whey explains the build-up of a deposit over and within the membrane which contributes to the decline in flux after 1 h ultrafiltration. The relative importance of these phenomena was quantified.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Taddei C,Daufin G,Aimar P,Sanchez Vdoi
10.1002/bit.260340205subject
Has Abstractpub_date
1989-06-20 00:00:00pages
171-9issue
2eissn
0006-3592issn
1097-0290journal_volume
34pub_type
杂志文章abstract::The effects of dilution rates on the performance of a two-stage fermentation system for a recombinant Escherichia coli culture were studied. Dilution rate determines the apparent or averaged specific growth rate of a heterogeneous population of cells in the recombinant culture. The specific growth rate affects the gen...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260380805
更新日期:1991-10-20 00:00:00
abstract::Embryonic stem cells (ESCs) can differentiate into all somatic cell types, but the development of effective strategies to direct ESC fate is dependent upon defining environmental parameters capable of influencing cell phenotype. ESCs are commonly differentiated via cell aggregates referred to as embryoid bodies (EBs),...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22578
更新日期:2010-02-15 00:00:00
abstract::The efficiency of a versatile in vivo cascade involving a promiscuous alcohol dehydrogenase, obtained from a biodiversity search, and a Baeyer-Villiger monooxygenase was enhanced by the independent control of the production level of each enzyme to produce ε-caprolactone and 3,4-dihydrocoumarin. This goal was achieved ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27133
更新日期:2019-11-01 00:00:00
abstract::Autoflocculation and bioflocculation are considered to be the most promising means for the economical harvesting of microalgae. We have therefore studied these phenomena with cultures of Scenedesmus obliquus produced during biological tertiary wastewater treatment. The quantity of extracellular polymers produced durin...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260300707
更新日期:1987-11-01 00:00:00
abstract::Bioartificial livers (BALs) are a potentially effective countermeasure against liver failure, particularly in cases of acute or fulminant liver failure. It is hoped these devices can sustain a patient's liver function until recovery or transplant. However, no large-scale clinical trial has yet proven that BALs are par...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22765
更新日期:2010-08-15 00:00:00
abstract::We describe a novel procedure to estimate the net growth rate of biofilms on multiple substrates. The approach is based on diffusion-reaction mass balances for chemical species in a continuum biofilm model with reaction kinetics corresponding to a Double-Monod expression. This analytical model considers a heterogeneou...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25284
更新日期:2014-11-01 00:00:00
abstract::Polymers produced by horseradish-peroxidase-catalyzed coupling of phenols have been explored as potential substitutes for phenol-formaldehyde resins. To overcome low substrate solubilities and product molecular weights in water, enzymatic polymerizations in aqueous-organic mixtures have been examined. Peroxidase vigor...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260300106
更新日期:1987-07-01 00:00:00
abstract::Disulfide-rich peptides isolated from cone snails are of great interest as drug leads due to their high specificity and potency toward therapeutically relevant ion channels and receptors. They commonly contain the inhibitor cystine knot (ICK) motif comprising three disulfide bonds forming a knotted core. Here we repor...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25993
更新日期:2016-10-01 00:00:00
abstract::After periodate oxidation of its glycosidic component, invertase was covalently bound onto three types of modified solid supports: glycidyl methacrylate, styrene-divinylbenzene copolymers, and bead cellulose. Direct reaction of the invertase aldehyde groups that were formed with amino groups of the support and use of ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260261011
更新日期:1984-10-01 00:00:00
abstract::DNA/polyethylenimine (PEI) polyplexes are an important class of nonviral vectors. Although the conventional preparation method, bulk mixing, is straightforward, the formation of the DNA/PEI polyplexes is not well controlled. This work explores coaxial electrohydrodynamic spraying (EHDS) as a novel, alternative method ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22583
更新日期:2010-03-01 00:00:00
abstract::Foam separation may have potential for protein recovery. However, for foam separation to be a viable protein recovery technique it is important to demonstrate, not only that high enrichments and recoveries can be achieved for single proteins, but also that high enrichments and recoveries, together with selectivity of ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990205)62:3<291::aid-bit
更新日期:1999-02-05 00:00:00
abstract::Design of 3D scaffolds that can facilitate proper survival, proliferation, and differentiation of progenitor cells is a challenge for clinical applications involving large connective tissue defects. Cell migration within such scaffolds is a critical process governing tissue integration. Here, we examine effects of sca...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23027
更新日期:2011-05-01 00:00:00
abstract::Streamline Direct CST I is a new type of ion exchanger with multi-modal functional groups, specially designed for an expanded bed adsorption (EBA) process, which can capture directly the proteins from the high ionic strength feedstocks with a high binding capacity. In this study, an experimental study is carried out f...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20952
更新日期:2006-08-20 00:00:00
abstract::Co-culture of hepatocytes or hepatocyte spheroids with the supporting NIH3T3 in a 3D microcapsule formed with a hybrid natural/synthetic matrix has led to enhanced hepatocyte functions. We investigated the mechanism of the functional enhancement in co-culture with respect to the contributions of soluble factors and di...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20372
更新日期:2005-03-05 00:00:00
abstract::Two empirical models for the enzymatic hydrolysis of cellulose are used to analyze the same set of experimental data to determine if one model is superior to the other. Both models adequately describe the data. The parameters in both models may be correlated to the hydrolysis conditions of enzyme, substrate, and produ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260260818
更新日期:1984-08-01 00:00:00
abstract::The possibility of enhancing the biomass productivity of a continuous culture of Saccharomyces cerevisiae growing on a glucose-limited medium is addressed. An unstructured Monod-type model is first identified using steady-state data. The culture is subjected to step changes in dilution rate, and it is seen that the Mo...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260340603
更新日期:1989-09-01 00:00:00
abstract::This paper describes construction of a nanosensory device for amplified detection of biologically important amines as chemical signals. The device was inspired by a biological signal transduction system, and was fabricated on an artificial cell membrane through self-organization of the molecular components, such as a ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22521
更新日期:2010-01-01 00:00:00
abstract::The study was done to evaluate the efficacy of using reuterin produced by Lactobacillus reuteri to sanitize biological tissues. The microorganism tested in the study was Pseudomonas aeruginosa, a common cause of nosocomial biomaterial-related infections. The inhibitory effect of reuterin on P. aeruginosa for an inocul...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10764
更新日期:2003-10-20 00:00:00
abstract::In this study, omics-based analysis tools were used to explore the effect of glucose starvation and culture duration on monoclonal antibody (mAb) production in fed-batch CHO cell culture to gain better insight into how these parameters can be controlled to ensure optimal mAb productivity and quality. Titer and N-glyco...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25620
更新日期:2015-10-01 00:00:00
abstract::This article describes a methodology that implements a Markov decision process (MDP) optimization technique in a real time fed-batch experiment. Biological systems can be better modeled under the stochastic framework and MDP is shown to be a suitable technique for their optimization. A nonlinear input/output model is ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19970720)55:2<317::AID-BIT
更新日期:1997-07-20 00:00:00
abstract::A mixed culture of sulfate-reducing bacteria containing the species Desulfovibrio desulfuricans was used to study sulfate-reduction stoichiometry and kinetics using ethanol as the carbon source. Growth yield was lower, and kinetics were slower, for ethanol compared to lactate. Ethanol was converted into acetate and no...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:2000-12-05 00:00:00
abstract::A technology has been developed to achieve optimal attachment of adhesion-independent lymphocytes to microcarrier beads. The activation of T-lymphocytes by concanavalin A was tested under microgravity conditions in an experiment carried out in space during the first Spacelab Life Science Mission. Activation, measure...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260400815
更新日期:1992-10-20 00:00:00
abstract::The desired product of bioprocesses is often produced in particulate form, either as an inclusion body (IB) or as a crystal. Particle harvesting is then a crucial and attractive form of product recovery. Because the liquid phase often contains other bioparticles, such as cell debris, whole cells, particulate biocataly...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20885
更新日期:2006-07-05 00:00:00
abstract::Two instruments were developed to mechanize the handling of anaerobic microorganisms for microbial mutant isolation. The instruments automatically dispense liquid or agar medium to large and small 96-well platesand petridishes. Protocols were developed for inoculating different microorganisms, and calibration curves o...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280905
更新日期:1986-09-01 00:00:00
abstract::The mouse mammary tumor virus (MMTV) promoter is induced by the addition of a glucocorticoid hormone or analog such as dexamethasone. The hormone binds to its specific transcription factor, glucocorticoid receptor (GR), and the activated complex then binds to the glucocorticoid response elements (GREs) in the enhancer...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:2000-01-20 00:00:00
abstract::The application of enantioseparation methods alone can only yield up to 50% of the desired chiral product. Thus enantioseparation becomes more attractive when accompanied by the racemization of the counter-enantiomer. Here we present first results of dynamic kinetic resolution of L-asparagine (L-Asn) via preferential ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22498
更新日期:2009-12-15 00:00:00
abstract::Retroviral-derived biopharmaceuticals (RV) target numerous therapeutic applications, from gene therapy to virus-like particle (rVLP)-based vaccines. During particle formation, beside the pseudotyped envelope proteins, RV can incorporate proteins derived from the virus producer cells (VPC). This may be detrimental by r...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23231
更新日期:2011-11-01 00:00:00
abstract::Neural stem cells (NSCs) are undifferentiated, primitive cells with important potential applications including the replacement of neural tissue lost due to neurodegenerative diseases, including Parkinson's disease, as well as brain and spinal cord injuries, including stroke. We have developed methods to rapidly expand...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20246
更新日期:2004-11-05 00:00:00
abstract::Gene deletion strategies using flux balance analysis (FBA) have improved the growth-coupled production of various compounds. However, the productivities were often below the expectation because the cells failed to adapt to these genetic perturbations. Here, we demonstrate the productivity of the succinate of the desig...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26568
更新日期:2018-06-01 00:00:00
abstract::The sufficient provision of oxygen is mandatory for enzymatic oxidations in aqueous solution, however, in process optimization this still is a bottleneck that cannot be overcome with the established methods of macrobubble aeration. Providing higher mass transfer performance through microbubble aerators, inefficient ae...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27556
更新日期:2021-01-01 00:00:00