Cell cycle kinetics of expanding populations of neural stem and progenitor cells in vitro.

abstract：:We report the first direct images of the hydration of protein powders. Using an environmental scanning electron microscope (ESEM) we have taken a series of micrographs of a region of the enzyme (subtilisin) power whilst hydrating the sample. In addition, the sample has been viewed during exposure to toluene vapors. Th...

journal_title：Biotechnology and bioengineering

authors： Roziewski K,Russell AJ

更新日期：1992-05-01 00:00:00

abstract：:Three cell lines, that is, the human breast cancer cell line (MCF-7) and the human mammary epithelial cell line (S-1) and its malignant form (T4-2) were embedded in a reconstituted basement membrane (Matrigel) that had 20-nL pyramid-shaped silicon microstructures. The proliferative behavior of the MCF-7 cells was depe...

journal_title：Biotechnology and bioengineering

authors： Torisawa YS,Nashimoto Y,Yasukawa T,Shiku H,Matsue T

更新日期：2007-06-15 00:00:00

abstract：:A flow cytometric kinetic study of hybridoma growth and monoclonal antibody production is presented, along with the influence of glutamine on intracellular responses such as (relative) cell size, and cell RNA and total protein content. Specific findings are: (1) RNA content remained constant throughout the growth phas...

journal_title：Biotechnology and bioengineering

authors： Dalili M,Ollis DF

更新日期：1990-06-05 00:00:00

abstract：:The effect of organic solvents on the equilibrium position of lipase-catalyzed esterification of glycerol and decanoic acid has been investigated. The reaction is carried out in an aqueous-organic two-phase system. In polar solvents, high mole fractions of monoacylglycerol and low mole fractions of triacylglycerol and...

journal_title：Biotechnology and bioengineering

authors： Janseen AE,Van der Padt A,Van Sonsbeek HM,Van't Riet K

更新日期：1993-01-05 00:00:00

abstract：:The rate of celluose degradation, limited due to the inhibition by cellobiose, can be increased by the hydrolysis of cellobiose to glucose using immobilized beta-glucosidase. Production of beta-glucosidase in four yeasts was studied and a maximum activity of 1.22 IU/mg cells was obtained in cells of Pichia etchellsii ...

journal_title：Biotechnology and bioengineering

authors： Jain D,Ghose TK

更新日期：1984-04-01 00:00:00

abstract：:We report the isolation of mutant strains of the methylotrophic yeast Hansenula polymorpha that are able to efficiently oxidize ethanol to acetaldehyde in an intact cell system. The oxidation reaction is catalyzed by alcohol oxidase (AOX), a key enzyme in the methanol metabolic pathway that is typically present only i...

journal_title：Biotechnology and bioengineering

authors： Moroz OM,Gonchar MV,Sibirny AA

更新日期：2000-04-05 00:00:00

abstract：:Exchange of enzyme-bound H(2)O with T(2)O in aqueous solution followed by freeze drying provided tritiated water bound to chymotrypsin, subtilisin Carlsberg, and horseradish peroxidase. The desorption of T(2)O from these enzymes suspended in various organic solvents showed that all three enzymes lost enzyme-bound wate...

journal_title：Biotechnology and bioengineering

authors： Gorman LA,Dordick JS

更新日期：1992-02-20 00:00:00

abstract：:Water permeability (Lp), calculated from the volume variations of cells subjected to an osmotic shock, is classically used to characterize cell membrane properties. In this work, we have shown the importance of the kind of mixing reactor used to measure the Lp parameter. A mathematical model including the mixing time ...

journal_title：Biotechnology and bioengineering

authors： de Marañón IM,Gervais P,Molin P

更新日期：1997-10-05 00:00:00

abstract：:We have produced Bioglass coatings for Orthopedic implants by using a novel coating technique, CoBlast. The two resultant surfaces, designated BG and hydroxyapatite (HA)/BG, were compared with their HA counterpart, OsteoZip in terms of osteoblastic cell attachment, adhesion, proliferation, differentiation, and growth ...

journal_title：Biotechnology and bioengineering

authors： Tan F,Naciri M,Al-Rubeai M

更新日期：2011-02-01 00:00:00

abstract：:Engineering protein expression in vitro or in vivo is usually straightforward for single genes, but remains challenging for multiple genes because of the requirement of coordinated control. RNA and protein overexpression strategies often exploit T7 RNA polymerase and its natural TPhi Class I terminator. However, this ...

journal_title：Biotechnology and bioengineering

authors： Du L,Gao R,Forster AC

更新日期：2009-12-15 00:00:00

abstract：:This paper presents a new formulation for the analysis of growth kinetics on multiple nutrients. The baseline for the theory is the concept that a noninteractive growth process occurs among perfectly substitutable nutrients if the locus of points of the substrate concentrations producing equal growth rate is linear. A...

journal_title：Biotechnology and bioengineering

authors： Haas CN

更新日期：1994-06-20 00:00:00

abstract：:A modified mixing plane approach for steady state simulation of flow field in fully baffled biological reactor is presented and discussed. Without requiring any experimental input, this approach of dividing the vessel into suitable number of connected and disconnected zones; solving steady state equation separately in...

journal_title：Biotechnology and bioengineering

authors： Dubey H,Das SK,Panda T

更新日期：2006-11-05 00:00:00

abstract：:Proteolysis associated with recombinant protein expression in Chinese Hamster Ovary (CHO) cells has hindered the development of biologics including HIV vaccines. When expressed in CHO cells, the recombinant HIV envelope protein, gp120, undergoes proteolytic clipping by a serine protease at a key epitope recognized by ...

journal_title：Biotechnology and bioengineering

authors： Li SW,Yu B,Byrne G,Wright M,O'Rourke S,Mesa K,Berman PW

更新日期：2019-09-01 00:00:00

abstract：:Biomass autoflocculation in outdoor algal cultures was found to be associated with increases of culture pH levels, due to CO(2) consumption by the algal photosynthetic activity. Under these alkaline conditions, some medium chemical ions precipitated together with the algal biomass. The chemical substances involved wit...

journal_title：Biotechnology and bioengineering

authors： Sukenik A,Shelef G

更新日期：1984-02-01 00:00:00

abstract：:Stability of heterologous protein expression during production is critical for regulatory approval of vaccine and therapeutic products. Leishmania GP63, a zinc metalloproteinase that is a potential vaccine candidate, has been expressed on the surface of Chinese hamster ovary (CHO) cells. Flow cytometry was used to fol...

journal_title：Biotechnology and bioengineering

authors： Morrison CJ,McMaster WR,Piret JM

更新日期：1997-03-20 00:00:00

abstract：:Constitutive activation of Wnt signaling plays an important role in colorectal and liver tumorigenesis. Cell-based assays using synthetic TCF/LEF (T-cell factor/lymphoid enhancer factor) reporters, as readouts of β-catenin/TCF-dependent transcriptional activity, have contributed greatly to the discovery of small molec...

journal_title：Biotechnology and bioengineering

authors： Yamaguchi K,Zhu C,Ohsugi T,Yamaguchi Y,Ikenoue T,Furukawa Y

更新日期：2017-12-01 00:00:00

abstract：:Intrinsic models, which take into account biomass volume fraction, must be formulated for adequate simulation of high-biomass-density fermentations with cell recycle. Through comparison of corresponding intrinsic and non-intrinsic models in dimensionless form, constraints for non-intrinsic model usage in terms of biok...

journal_title：Biotechnology and bioengineering

authors： Monbouquette HG

更新日期：1987-06-01 00:00:00

abstract：:Potentiometric and amperometric measurements were made with microbial fuel cells containing E. coli or yeast as the anodic reducing agent and glucose as the oxidizable substrate. The catalytic effects of thionine and resorufin on the anode reaction were investigated. Results on the potentiometry, polarization, and cou...

journal_title：Biotechnology and bioengineering

authors： Bennetto HP,Stirling JL,Tanaka K,Vega CA

更新日期：1983-02-01 00:00:00

abstract：:Mathematical modeling of animal cell growth and metabolism is essential for the understanding and improvement of the production of biopharmaceuticals. Models can explain the dynamic behavior of cell growth and product formation, support the identification of the most relevant parameters for process design, and signifi...

journal_title：Biotechnology and bioengineering

authors： Ramos JRC,Rath AG,Genzel Y,Sandig V,Reichl U

更新日期：2020-05-01 00:00:00

abstract：:Chlorella sp. strain VJ79 was isolated from a total heterotrophic count of a wastewater collector. It grows autotrophically, heterotrophically, and mixotrophically on a variety of organic substrates. Glucose and serine promote a mixotrophic growth from which the yield is higher than the sum of autotrophic and heterotr...

journal_title：Biotechnology and bioengineering

authors： Lalucat J,Imperial J,Parés R

更新日期：1984-07-01 00:00:00

abstract：:The production of Bacillus thuringiensis spores was investigated in a bioreactor incorporating a ceramic membrane filter to improve spore concentration and volumetric productivity. Two cultivation methods were used in this study: a total cell retention culture (TCRC), and a two-stage continuous culture with partial ce...

journal_title：Biotechnology and bioengineering

authors： Kang BC,Lee SY,Chang HN

更新日期：1993-11-05 00:00:00

abstract：:In macroscopic dynamic models of fermentation processes, elementary modes (EM) derived from metabolic networks are often used to describe the reaction stoichiometry in a simplified manner and to build predictive models by parameterizing kinetic rate equations for the EM. In this procedure, the selection of a set of EM...

journal_title：Biotechnology and bioengineering

authors： Hebing L,Neymann T,Engell S

更新日期：2020-07-01 00:00:00

abstract：:A kinetic model for anaerobic digestion of insoluble solid-state substrates was developed. Rate equations for cell growth and substrate consumption were derived based on the assumption that the microorganisms assimilate the substrate mainly at the point of contact where they grow. The model emphasizes effects of subst...

journal_title：Biotechnology and bioengineering

authors： Huang SY,Chou MS

更新日期：1990-03-15 00:00:00

abstract：:PHA, a naturally occurring biopolymer produced by a wide range of microorganisms, is known for its applications as bioplastic. In recent years the use of agro-industrial wastewater as substrate for PHA production by bacterial enrichments has attracted considerable research attention. Crude glycerol as generated during...

journal_title：Biotechnology and bioengineering

authors： Moralejo-Gárate H,Palmeiro-Sánchez T,Kleerebezem R,Mosquera-Corral A,Campos JL,van Loosdrecht MC

更新日期：2013-12-01 00:00:00

abstract：:In a stirred culture of hybridoma cells, the effects of serum reduction from 2.5% to 0% on growth and monoclonal antibody production have been investigated. The shear sensitivity of cells from the same culture has been tested in a bubble column. Serum reduction does not greatly affect viable-cell concentrations, but c...

journal_title：Biotechnology and bioengineering

authors： van der Pol L,Bakker WA,Tramper J

更新日期：1992-06-05 00:00:00

abstract：:We have shown previously that a sequence-specific DNA-binding protein based on the Lac repressor protein can isolate pre-purified DNA efficiently from simple buffer solution but our attempts to purify plasmids directly from crude starting materials were disappointing with impractically low DNA yields. We have optimize...

journal_title：Biotechnology and bioengineering

authors： Darby RA,Forde GM,Slater NK,Hine AV

更新日期：2007-12-01 00:00:00

abstract：:The present study evaluated the influence of water activity and lactose concentration on the synthesis of galactooligosaccharides (GOS), by means of a hyperthermophilic beta-glycosidase in an organic system. The production of GOS gradually grew as water activity increased in the reaction system; later, their synthesis...

journal_title：Biotechnology and bioengineering

authors： Cruz-Guerrero AE,Gómez-Ruiz L,Viniegra-González G,Bárzana E,García-Garibay M

更新日期：2006-04-20 00:00:00

abstract：:Direct perfusion of 3D tissue engineered constructs is known to enhance osteogenesis, which can be partly attributed to enhanced nutrient and waste transport. In addition flow mediated shear stresses are known to upregulate osteogenic differentiation and mineralization. A quantification of the hydrodynamic environment...

journal_title：Biotechnology and bioengineering

authors： Maes F,Van Ransbeeck P,Van Oosterwyck H,Verdonck P

更新日期：2009-06-15 00:00:00

abstract：:The objective of this work was to relate macroscopically measurable on-line fermentation parameters such as dissolved oxygen, off-gas oxygen and carbon dioxide, and cell mass, to the controlled production of key intracellular enzymes under carbon limited conditions. Both batch and perturbed batch aerobic fermentations...

journal_title：Biotechnology and bioengineering

authors： Pih N,De Bernardez E,Dhurjati P

更新日期：1988-03-01 00:00:00

abstract：:Real-time polymerase chain reaction (PCR) is one of the most sensitive and accurate methods for quantifying transcript levels especially for those expressed at low abundance. The selective amplification of target DNA over multiple cycles allows its initial concentration to be determined. The amplification rate is a co...

journal_title：Biotechnology and bioengineering

authors： Mehra S,Hu WS

更新日期：2005-09-30 00:00:00