Abstract:
:The objective of this work was to relate macroscopically measurable on-line fermentation parameters such as dissolved oxygen, off-gas oxygen and carbon dioxide, and cell mass, to the controlled production of key intracellular enzymes under carbon limited conditions. Both batch and perturbed batch aerobic fermentations were performed using two different strains of Escherichia coli, with glucose and lactose as the sole carbon sources. The two strains differed from each other only in the lac operon region of their genome. The parent strain, E. coli 3000, was inducible for the enzyme beta-galactosidase. The other strain, E. coli 3300, was a constitutive mutant in the production of beta-galactosidase. In all experiments, off-line assays of sugars and beta-galactosidase activity were performed. It was observed that there is a clear relationship between the macroscopic on-line measurements, dissolved oxygen tension, carbon dioxide evolution rate and oxygen uptake rate, and the microscopic control phenomena of catabolite repression, catabolite inhibition, and inducer repression.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Pih N,De Bernardez E,Dhurjati Pdoi
10.1002/bit.260310406subject
Has Abstractpub_date
1988-03-01 00:00:00pages
311-20issue
4eissn
0006-3592issn
1097-0290journal_volume
31pub_type
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21080
更新日期:2006-12-05 00:00:00
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journal_title:Biotechnology and bioengineering
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doi:10.1002/bit.260370112
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260290110
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更新日期:2005-09-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260391114
更新日期:1992-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-01-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1994-06-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260290906
更新日期:1987-06-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1990-04-25 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:1985-05-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2014-04-01 00:00:00
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pub_type: 信件
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更新日期:2011-04-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1999-06-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21711
更新日期:2008-04-15 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1987-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-09-01 00:00:00
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journal_title:Biotechnology and bioengineering
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pub_type: 杂志文章
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