Abstract:
:Water permeability (Lp), calculated from the volume variations of cells subjected to an osmotic shock, is classically used to characterize cell membrane properties. In this work, we have shown the importance of the kind of mixing reactor used to measure the Lp parameter. A mathematical model including the mixing time constant has been proposed allowing an accurate Lp estimation even though the mixing time constant is higher than the cell time constant obtained in response to a perfect shock. The estimated Lp values of human leukemia K562 cells were found to be the same whatever the mixing time constant. The Lp value of Saccharomyces cerevisiae could not be exactly estimated. However, S. cerevisiae has unexpectedly high water permeability, implying that this yeast may contain water channels in the membrane.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
de Marañón IM,Gervais P,Molin Pdoi
10.1002/(SICI)1097-0290(19971005)56:1<62::AID-BIT7subject
Has Abstractpub_date
1997-10-05 00:00:00pages
62-70issue
1eissn
0006-3592issn
1097-0290journal_volume
56pub_type
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