Identification and CRISPR/Cas9 Inactivation of the C1s Protease Responsible for Proteolysis of Recombinant Proteins Produced in CHO Cells.

abstract：:NS0, a nonsecreting mouse myeloma cell, is a major host line used for recombinant antibody production. These cells have a cholesterol-dependent phenotype and rely on an exogenous supply of cholesterol for their survival and growth. To better understand the physiology underlying cholesterol dependence, we compared NS0 ...

journal_title：Biotechnology and bioengineering

authors： Seth G,Philp RJ,Denoya CD,McGrath K,Stutzman-Engwall KJ,Yap M,Hu WS

更新日期：2005-06-05 00:00:00

abstract：:Light-responsive bioconjugates are synthesized by a two-step protocol calling first for cotelomerization (chain-transfer polymerization) of N-isopropylacrylamide and N-acryloxysuccinimide. The desired bioligand (biotin) is used in modified form as chain-transfer agent in this step. As a consequence, 100% of the produc...

journal_title：Biotechnology and bioengineering

authors： Desponds A,Freitag R

更新日期：2005-09-05 00:00:00

abstract：:In the last few years, an increasing number of biotechnological techniques have been applied to the restoration and conservation of works of art, paintings, old maps, and papers or books. Enzymes can solve problems that give restorers difficulties, although for many applications it is not possible to use soluble enzym...

journal_title：Biotechnology and bioengineering

authors： Moeschel K,Nouaimi M,Steinbrenner C,Bisswanger H

更新日期：2003-04-20 00:00:00

abstract：:The effect of organic solvents on the equilibrium position of lipase-catalyzed esterification of glycerol and decanoic acid has been investigated. The reaction is carried out in an aqueous-organic two-phase system. In polar solvents, high mole fractions of monoacylglycerol and low mole fractions of triacylglycerol and...

journal_title：Biotechnology and bioengineering

authors： Janseen AE,Van der Padt A,Van Sonsbeek HM,Van't Riet K

更新日期：1993-01-05 00:00:00

abstract：:Gene expression circuitries with time-delayed expression profiles regulate key events, such as oscillating systems, noise elimination, and coordinated multi-step processes, in all organisms from bacteria to mammalian cells. We present the rational synthesis of a genetic circuit displaying time-delayed expression in si...

journal_title：Biotechnology and bioengineering

authors： Weber W,Kramer BP,Fussenegger M

更新日期：2007-11-01 00:00:00

abstract：:The behavior of 20 beta-hydroxysteroid dehydrogenase in a two-phase system consisting of an emulsion of water with an immiscible organic solvent was investigated. The effect of several organic solvents on the stability, activity, and kinetic profile of the enzyme was considered. The most suitable system for carrying o...

journal_title：Biotechnology and bioengineering

authors： Cremonesi P,Carrea G,Ferrara L,Antonini E

更新日期：1975-08-01 00:00:00

abstract：:We have developed a dual-chambered bioreactor (DCB) that incorporates a membrane to study stratified 3D cell populations for skin tissue engineering. The DCB provides adjacent flow lines within a common chamber; the inclusion of the membrane regulates flow layering or mixing, which can be exploited to produce layers o...

journal_title：Biotechnology and bioengineering

authors： Navarro J,Swayambunathan J,Janes ME,Santoro M,Mikos AG,Fisher JP

更新日期：2019-12-01 00:00:00

abstract：:Multiparameter flow cytometric techniques developed in our laboratories have been used for the "at-line" study of fed-batch bacterial fermentations. These fermentations were done at two scales, production (20 m(3)) and bench (5 x 10(-3) m(3)). In addition, at the bench scale, experiments were undertaken where the diff...

journal_title：Biotechnology and bioengineering

authors： Hewitt CJ,Nebe-Von Caron G,Axelsson B,McFarlane CM,Nienow AW

更新日期：2000-11-20 00:00:00

abstract：:A MATLAB-based program was developed to simulate the distributions of states and behaviors of microbial storage product-accumulating bacteria in suspended growth systems. Currently available computer simulators of these systems predict dynamic behaviors by numerically solving differential biokinetic equations using av...

journal_title：Biotechnology and bioengineering

authors： Schuler AJ

更新日期：2005-07-05 00:00:00

abstract：:The long hydrocarbon fatty acyl chain is energy rich, making it an ideal precursor for liquid transportation fuels and high-value oleo chemicals. As Saccharomyces cerevisiae has many advantages for industrial production compared to Escherichia coli. Here, we attempted to engineer Saccharomyces cerevisiae for overprodu...

journal_title：Biotechnology and bioengineering

authors： Li X,Guo D,Cheng Y,Zhu F,Deng Z,Liu T

更新日期：2014-09-01 00:00:00

abstract：:Self-cycling fermentation (SCF), a cyclical, semi-continuous process that induces cell synchrony, was incorporated into a recombinant protein production scheme. Escherichia coli CY15050, a lac(-) mutant lysogenized with temperature-sensitive phage λ modified to over-express β-galactosidase, was used as a model system....

journal_title：Biotechnology and bioengineering

authors： Storms ZJ,Brown T,Sauvageau D,Cooper DG

更新日期：2012-09-01 00:00:00

abstract：:Biofilms commonly develop in flowing aqueous environments, where the flow causes the biofilm to deform. Because biofilm deformation affects the flow regime, and because biofilms behave as complex heterogeneous viscoelastic materials, few models are able to predict biofilm deformation. In this study, a phase-field (PF)...

journal_title：Biotechnology and bioengineering

authors： Li M,Matouš K,Nerenberg R

更新日期：2020-07-13 00:00:00

abstract：:The effects of low intensity pulsed ultrasound (LIPUS) on proliferation and chondrogenic differentiation of human mesenchymal stem cells (hMSCs) seeded on 3D printed poly-(ethylene glycol)-diacrylate (PEG-DA) scaffolds with varying pore geometries (square and hexagonal channels) were investigated. The scaffold with sq...

journal_title：Biotechnology and bioengineering

authors： Aliabouzar M,Lee SJ,Zhou X,Zhang GL,Sarkar K

更新日期：2018-02-01 00:00:00

abstract：:Microparticle-enhanced cultivation (MPEC) was applied as a novel method for improved biomass and product formation during cultivation of filamentous microorganisms. Exemplarily, chloroperoxidase (CPO) formation by Caldariomyces fumago was analyzed in the presence and absence of microparticles of different size. Partic...

journal_title：Biotechnology and bioengineering

authors： Kaup BA,Ehrich K,Pescheck M,Schrader J

更新日期：2008-02-15 00:00:00

abstract：:Utilising whole cells of recombinant Escherichia coli K27 (pCYP102, pGEc47) containing active cytochrome P450BM-3 monooxygenase [E.C. 1. 14.14.1], multiple oxidations of saturated and unsaturated fatty acids were performed by the enzyme under conditions of excess oxygen. The amount of oxygen dissolved in the culture m...

journal_title：Biotechnology and bioengineering

authors： Schneider S,Wubbolts MG,Oesterhelt G,Sanglard D,Witholt B

更新日期：1999-08-05 00:00:00

abstract：:Genetically transformed shooty teratomas of Atropa belladonna and a Duboisia leichhardtii x D. myoporoides hybrid were studied for biotransformation of tropane alkaloids in shake flasks and bioreactors. Although de novo synthesis of hyoscyamine and scopolamine was limited, shoots of both species were able to transloca...

journal_title：Biotechnology and bioengineering

authors： Subroto MA,Kwok KH,Hamill JD,Doran PM

更新日期：1996-03-05 00:00:00

abstract：:There are a series of examples in which phenols appear as contaminants in process streams and their selective removal is required for waste minimization. For the selective removal of a phenol from a mixture, we are exploiting the substrate specificity of the enzyme tyrosinase to convert phenols into reactive o-quinone...

journal_title：Biotechnology and bioengineering

authors： Sun WQ,Payne GF

更新日期：1996-07-05 00:00:00

abstract：:Oxygen and nutrient limitation was investigated in order to identify the origin of a lower specific ajmalicine production in Catharanthus roseus cultures at high cell densities in an induction medium. The effect of oxygen limitation was explored by comparing two identically aerated and agitated high cell density biore...

journal_title：Biotechnology and bioengineering

authors： Schlatmann JE,Moreno PR,Vinke JL,Ten Hoopen HJ,Verpoorte R,Heijnen JJ

更新日期：1994-08-05 00:00:00

abstract：:With a view toward reduction of graft loss, we explored pancreatic islet transplantation within fibrin matrices rendered pro-angiogenic by incorporation of minimal doses of vascular endothelial growth factor-A165 and platelet-derived growth factor-BB presented complexed to a fibrin-bound integrin-binding fibronectin d...

journal_title：Biotechnology and bioengineering

authors： Najjar M,Manzoli V,Abreu M,Villa C,Martino MM,Molano RD,Torrente Y,Pileggi A,Inverardi L,Ricordi C,Hubbell JA,Tomei AA

更新日期：2015-09-01 00:00:00

abstract：:Cell culture longevity in fed-batch culture of hybridomas is often limited by elevated medium osmolality caused by repeated nutrient feeding. Shotwise feeding of 10x Dulbecco's modified Eagle's medium (DMEM) concentrates elevated the osmolality of medium up to 540 mOsm/kg at the end of fed-batch culture of S3H5/gamma2...

journal_title：Biotechnology and bioengineering

authors： Ryu JS,Lee GM

更新日期：1999-01-05 00:00:00

abstract：:A novel strategy for the maximum production of a biodegradable copolymer, poly(3-hydroxybutyric-co-hydroxyvaleric) acid, P(HB-co-HV), was developed, based on the kinetic parameters obtained from fed-batch culture experiments of Alcaligenes eutrophus. The effects of various culture conditions such as mole ratio of carb...

journal_title：Biotechnology and bioengineering

authors： Shimizu H,Kozaki Y,Kodama H,Shioya S

更新日期：1999-03-05 00:00:00

abstract：:The glycosylation of pharmaglycoproteins from recombinant cell lines can be affected by an uncontrolled accumulation of ammonium in the medium. Glucosamine-6-phosphate isomerase (GPI) has been proposed as the key enzyme responsible for elevating the intracellular UDP-N-acetylhexosamine pool (UDPGNAc) by accepting ammo...

journal_title：Biotechnology and bioengineering

authors： Cayli A,Hirschmann F,Wirth M,Hauser H,Wagner R

更新日期：1999-10-20 00:00:00

abstract：:The stoichiometry and kinetics of the spontaneous, chemical reaction between pyrite and ferric iron was studied at 30, 45, and 70 degrees C in shake flasks at pH 1.5 by monitoring the ferrous iron, total iron, elemental sulfur, and sulfate concentration profiles in time. It was found that the sulfur moiety of pyrite w...

journal_title：Biotechnology and bioengineering

authors： Boogerd FC,van den Beemd C,Stoelwinder T,Bos P,Kuenen JG

更新日期：1991-06-20 00:00:00

abstract：:Using phage display technology, we have isolated 12-mer peptide ligands that bind to human blood outgrowth endothelial cells (HBOEC). To avoid non-specific binding we apply negative-positive selection approach by pre-incubating the library with non-HBOEC. The selected phage clones bind to their target cell population ...

journal_title：Biotechnology and bioengineering

authors： Veleva AN,Cooper SL,Patterson C

更新日期：2007-09-01 00:00:00

abstract：:We developed a kinetic model that describes a heterogeneous reaction system for the production of D-p-hydroxyphenylglycine from D,L-p-hydroxyphenyl-hydantoin using D-hydantoinase of Bacillus stearothermophilus SD1 and N-carbamoylase of Agrobacterium tumefaciens NRRL B11291. As a biocatalyst, whole cells with separatel...

journal_title：Biotechnology and bioengineering

authors： Park JH,Oh KH,Lee DC,Kim HS

更新日期：2002-06-30 00:00:00

abstract：:Two attractive features of ELISA are the specificity of antibody-antigen recognition and the sensitivity achieved by enzymatic amplification. This report describes the development of a non-enzymatic molecular recognition platform adaptable to point-of-care clinical settings and field detection of biohazardous material...

journal_title：Biotechnology and bioengineering

authors： Stone GP,Mernaugh R,Haselton FR

更新日期：2005-09-20 00:00:00

abstract：:Engineered human T-cells are a promising therapeutic modality for cancer immunotherapy. T-cells expressing chimeric antigen receptors combined with additional genes to enhance T-cell proliferation, survival, or tumor targeting may further improve efficacy but require multiple stable gene transfer events. Methods are t...

journal_title：Biotechnology and bioengineering

authors： Kacherovsky N,Liu GW,Jensen MC,Pun SH

更新日期：2015-07-01 00:00:00

abstract：:This study deals with the use of an upward gas/liquid slug flow to reduce tubular mineral membrane fouling. The injection of air into the feedstream is designed to create hydrodynamic conditions that destabilize the cake layer over the membrane surface inside the filtration module complex. Experimental study was carri...

journal_title：Biotechnology and bioengineering

authors： Mercier M,Maranges C,Fonade C,Lafforgue-Delorme C

更新日期：1998-04-05 00:00:00

abstract：:Anaerobic digestion of biological organic particulates to methane has been described by a structured mathematical model based on multiple-reaction stoichiometry, conventional material balances, and liquid phase equilibrium chemistry. A general stoichiometric treatment for any set of multiple biological reactions is de...

journal_title：Biotechnology and bioengineering

authors： Bryers JD

更新日期：1985-05-01 00:00:00

abstract：:Point and 95% confidence interval estimates of the true growth yield and maintenance coefficient are reported for four pure cultures andtwo mixed cultures of Lactobacillus bulgaricus and Streptococcus thermophilus grown anaerobically on 3% nonfat dry milk using batch followed by fed batch culture. An improved method o...

journal_title：Biotechnology and bioengineering

authors： Oner MD,Erickson LE,Yang SS

更新日期：1986-06-01 00:00:00