Abstract:
:A flow cytometric kinetic study of hybridoma growth and monoclonal antibody production is presented, along with the influence of glutamine on intracellular responses such as (relative) cell size, and cell RNA and total protein content. Specific findings are: (1) RNA content remained constant throughout the growth phase, then fell drastically as the cells entered the stationary phase. Also, in stationary phase, RNA content of antibody-producing cells was higher than for those not secreting antibody. (2) The cell size was constant and maximal throughout exponential phase, and diminished monotonically during later stages. (3) Average protein and antibody cellular content declined dramatically upon glutamine exhaustion. Thus, relative RNA levels and cell size provided quantitative determinants of both cell growth state and antibody secretion conditions. These results encourage consideration of structured kinetic studies which recognize the quality of the biophase.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Dalili M,Ollis DFdoi
10.1002/bit.260360109subject
Has Abstractpub_date
1990-06-05 00:00:00pages
64-73issue
1eissn
0006-3592issn
1097-0290journal_volume
36pub_type
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