Abstract:
:Porcine pancreatic lipase and Fusarium solani cutinase were used to catalyze transesterification reactions between methyl propionate, ethyl propionate, and a series of primary alcohols at high temperatures in a continuous packed-bed gas-solid reactor, in which the solid phase is composed of the enzyme and the substrates and products are in a gaseous form. In this type of system, enzyme activity was found to depend essentially on the water activity (A(w)) of the enzyme preparation.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Parvaresh F,Robert H,Thomas D,Legoy MDdoi
10.1002/bit.260390415subject
Has Abstractpub_date
1992-02-20 00:00:00pages
467-73issue
4eissn
0006-3592issn
1097-0290journal_volume
39pub_type
杂志文章abstract::Bifidobacterium animalis ATCC 27536 represents among bifidobacteria a host-model for cloning experiments. The segregational and structural stabilities of a family of cloning vectors with different molecular weights but sharing a common core were studied in continuous fermentation of the hosting B. animalis without sel...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10737
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260300307
更新日期:1987-08-20 00:00:00
abstract::Trypsin and alpha-chymotrypsin were immobilized by gelentrapment in polyacrylamide cross-linked with N,N(1)-methylenebisacrylamide. The immobilized enzymes are catalytically efficient in suspensions of reverse micelles formed in isooctane by bis(2-ethylhexyl) sodium sulfosuccinate (AOT) and water. Both entrapped enzym...
journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26598
更新日期:2018-07-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27575
更新日期:2021-01-01 00:00:00
abstract::Recent environmental concerns have prompted a re-evaluation of conventional management strategies and refueled the search of innovative waste management practices. In this sense, the anaerobic digestion of both fat and the remaining complex organic matter present in dairy wastewaters is attractive, although the contin...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21095
更新日期:2007-02-01 00:00:00
abstract::Phosphite dehydrogenase represents a new enzymatic system for regenerating reduced nicotinamide cofactors for industrial biocatalysis. We previously engineered a variant of phosphite dehydrogenase with relaxed cofactor specificity and significantly increased activity and stability. Here we performed one round of rando...
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260430811
更新日期:1994-04-05 00:00:00
abstract::Tissue function is comprised of a complex interplay between biological and physicochemical rate processes. The design of bioreactors for tissue engineering must account for these processes simultaneously in order to obtain a bioreactor that provides a uniform environment for tissue growth and development. In the prese...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1996-06-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2020-08-01 00:00:00
abstract::Using solid-phase organic synthesis, we have prepared a 432-member (18 x 8 x 3) sample library based on the AG 490 "tyrphostin" template. By utilizing 432 reactors each equipped with a unique radiofrequency memory ID tag, the 432 products could be obtained as discrete entities (i.e., not as mixtures) via 18 + 8 + 3, o...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1998-01-01 00:00:00
abstract::The utilization and conversion of D-xylose, D-xylulose, L-arabinose, and xylitol by yeast strains have been investigated with the following results: (1) The majority of yeasts tested utilize D-xylose and produce polyols, ethanol, and organic acids. The type and amount of products formed varies with the yeast strains u...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260250108
更新日期:1983-01-01 00:00:00
abstract::Product inhibition of biological production systems is a widely observed phenomenon with prominent implications for the design and ultimately the success of biotechnological processes. In order to investigate whether such effects could limit the maximal concentrations in the production of the beta-lactamase inhibitor ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21783
更新日期:2008-06-15 00:00:00
abstract::By feeding ethanol at various high rates to low cell density cultures of Saccharomyces cerevisiae it was shown that the sharp fall in viability when ethanol is produced during rapid fermentations is in part a direct consequence of the high rate of change of extracellular ethanol concentration. Nevertheless, the fall i...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260350202
更新日期:1990-01-20 00:00:00
abstract::Experimental observations indicate that the rates of cometabolic transformation are linked to the consumption of growth substrate during growth and to the consumption of cell mass and/or energy substrate in the absence of growth substrate. Three previously proposed models (models 1 through 3) describing the kinetics o...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260411107
更新日期:1993-05-01 00:00:00
abstract::Knowledge of the aqueous phase methane concentration is critical to understanding and controlling process kinetics in methanotrophic bioreactors. Unfortunately since no dissolved methane probe is commercially available, this data must be obtained off-line by the time-consuming gas-liquid partition method. In this stud...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26829
更新日期:2018-12-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260380708
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260400115
更新日期:1992-06-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21437
更新日期:2007-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-01-20 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:1993-11-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20882
更新日期:2006-07-05 00:00:00
abstract::Ralstonia eutropha grows on and produces polyhydroxyalkanoates (PHAs) from fermentation acids. Acetic acid, one major organic acid from acidogenesis of organic wastes, has an inhibitory effect on the bacterium at slightly alkaline pH (6 g HAc/L at pH 8). The tolerance of R. eutropha to acetate, however, was increased ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1080
更新日期:2001-06-20 00:00:00
abstract::A process is described in which the baboon endogenous virus (BaEV) is produced under optimum conditions in cell culture, and concentrated by hollow-fiber ultrafiltration technology under conditions of large-scale production. This system has advantages over conventional systems in that the flow rate is increased 2.5-fo...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260220103
更新日期:1980-01-01 00:00:00
abstract::The production and specific productivity of hepatitis B surface antigen (HBsAg) in recombinant Chinese hamster ovary (CHO) cells were increased by 81% and threefold, respectively, when supplemented with 1.5% dimethyl sulfoxide (DMSO) in the culture medium. To investigate the mechanism of DMSO effect on HBsAg productio...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2007-06-15 00:00:00
abstract::Anaerobic digestion of biological organic particulates to methane has been described by a structured mathematical model based on multiple-reaction stoichiometry, conventional material balances, and liquid phase equilibrium chemistry. A general stoichiometric treatment for any set of multiple biological reactions is de...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270514
更新日期:1985-05-01 00:00:00
abstract::Direct perfusion of 3D tissue engineered constructs is known to enhance osteogenesis, which can be partly attributed to enhanced nutrient and waste transport. In addition flow mediated shear stresses are known to upregulate osteogenic differentiation and mineralization. A quantification of the hydrodynamic environment...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22277
更新日期:2009-06-15 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10540
更新日期:2003-03-30 00:00:00