Abstract:
:Perfusion bioreactors have been used in different tissue engineering applications because of their consistent distribution of nutrients and flow-induced shear stress within the tissue-engineering scaffold. A widely used configuration uses a scaffold with a circular cross-section enclosed within a cylindrical chamber and inlet and outlet pipes which are connected to the chamber on either side through which media is continuously circulated. However, fluid-flow experiments and simulations have shown that the majority of the flow perfuses through the center. This pattern creates stagnant zones in the peripheral regions as well as in those of high flow rate near the inlet and outlet. This non-uniformity of flow and shear stress, owing to a circular design, results in limited cell proliferation and differentiation in these areas. The focus of this communication is to design an optimized perfusion system using computational fluid dynamics as a mathematical tool to overcome the time-consuming trial and error experimental method. We compared the flow within a circular and a rectangular bioreactor system. Flow simulations within the rectangular bioreactor are shown to overcome the limitations in the circular design. This communication challenges the circular cross-section bioreactor configuration paradigm and provides proof of the advantages of the new design over the existing one.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Hidalgo-Bastida LA,Thirunavukkarasu S,Griffiths S,Cartmell SH,Naire Sdoi
10.1002/bit.24368subject
Has Abstractpub_date
2012-04-01 00:00:00pages
1095-9issue
4eissn
0006-3592issn
1097-0290journal_volume
109pub_type
杂志文章abstract::Nutrient utilization and byproduct accumulation were monitored in Spodoptera frugiperda Sf-9 and Trichoplusia ni BTI-Tn-5B1-4 (High Five) cell lines during growth and following viral infection in suspension cultures in order to develop a better understanding of cell metabolism and to acquire information relevant to la...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19970920)55:6<909::AID-BIT
更新日期:1997-09-20 00:00:00
abstract::The end-product profile of the glucose fermentation by Propionibacterium freudenreichii ET-3 changed on an electrochemical treatment, in which the culture vessel was filled with a carbon felt anode. Acetate and propionate were produced as final end products in a molar ratio of 2:3 without any electrochemical treatment...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21914
更新日期:2008-10-15 00:00:00
abstract::The C-terminal lysine variation is commonly observed in biopharmaceutical monoclonal antibodies. This modification can be important since it is found to be sensitive to the production process. The methods commonly used to probe this charge variation, including IEF, cIEF, ion-exchange chromatography, and LC-MS, were ev...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21855
更新日期:2008-08-15 00:00:00
abstract::Protein engineering is a powerful tool for improving the properties of enzymes. However, large changes in enzyme properties are still challenging for traditional evolution strategies because they usually require multiple amino acid substitutions. In this study, a feasible evolution approach by a combination of fragmen...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27203
更新日期:2020-02-01 00:00:00
abstract::The results of an initial study of enzymatic catalysis in metastable supersaturated solutions of carbohydrates are presented. It has been shown that such solutions, formed in the presence of small amounts of water and alcohol as plasticizers, are sufficiently stable under ambient conditions to enable enzymatic transfo...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1998-10-20 00:00:00
abstract::Hemicellulose residues can be hydrolyzed into a sugar syrup using dilute mineral acids. Although this syrup represents a potential feedstock for biofuel production, toxic compounds generated during hydrolysis limit microbial metabolism. Escherichia coli LY01, an ethanologenic biocatalyst engineered to ferment the mixe...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(1999)66:4<203::aid-bit1>3.
更新日期:1999-01-01 00:00:00
abstract::This paper presents an experimental analysis of matrix bead size distribution and voidage variations with axial height in an expanded bed adsorption system. Use of a specially constructed expanded bed with side ports has enabled sampling from within the expanded bed along the vertical axis. Particles removed from with...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/1097-0290(20000920)69:6<648::aid-bit9>3.0.
更新日期:2000-09-20 00:00:00
abstract::Three-dimensional (3D) tumor models have been established in various microfluidic systems for drug delivery and resistance studies in vitro. However, one of the main drawbacks of these models is non-uniform distribution of cells, leaving regions with very low cell density within the 3D structures. As a result, molecul...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24397
更新日期:2012-05-01 00:00:00
abstract::In this work, it was discovered that the stability of mRNA in a cell-free extract could be controlled by using engineered T7 terminator sequences. Specifically, it was found that mRNA stability gradually decreased as the length of the stem structure of the T7 terminator was reduced sequentially. As a result of the con...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21884
更新日期:2008-10-01 00:00:00
abstract::Polymeric membranes are increasingly used as supports for the immobilization of enzymes in bioreactors. One of the more common reactor types employed in lipase-catalyzed hydrolysis of oils, contains modified cellulose as a membrane material. We found that this type of material is readily attacked by cellulase present ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260400714
更新日期:1992-10-05 00:00:00
abstract::Modifications are made to an earlier thermodynamic model (TEEM1) for prediction of maximum microbial yields from aerobic and anaerobic as well as heterotrophic and autotrophic growth. The revised model (TEEM2) corrects for lower yields found with aerobic oxidations of organic compounds where an oxygenase is involved a...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21250
更新日期:2007-06-01 00:00:00
abstract::The naturally existing microbial hosts can rarely satisfy industrial requirements, thus there has always been an intense effort in strain engineering to meet the needs of these bioprocesses. Here, in this work, we want to prove the concept that engineering global transcription factor cAMP receptor protein (CRP) of Esc...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24411
更新日期:2012-05-01 00:00:00
abstract::Ethanol-producing bioreactors employing cells of Zymomonas mobilis attached to glass-fiber pads were operated continuously for as long as 28 days. Ethanol production, which is related to bed-associated biomass levels, was found to occur in three distinct phases: an exponential phase, a linear phase, and a "steady-stat...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260240306
更新日期:1982-03-01 00:00:00
abstract::Expanded bed adsorption is an integrated technology that allows the introduction of particle-containing feedstock without the risk of blocking the bed. The biomass particles contained in the feedstock have to be treated as an integral part of the process and potential interactions between suspended biomass and the ads...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10654
更新日期:2003-07-20 00:00:00
abstract::This article describes a methodology that implements a Markov decision process (MDP) optimization technique in a real time fed-batch experiment. Biological systems can be better modeled under the stochastic framework and MDP is shown to be a suitable technique for their optimization. A nonlinear input/output model is ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19970720)55:2<317::AID-BIT
更新日期:1997-07-20 00:00:00
abstract::Genetically-engineered cells offer a solution to the cell availability problem in tissue engineering a pancreatic substitute for the treatment of insulin-dependent diabetes. These cells can be non-beta cells, such as hepatocytes or myoblasts, retrieved as a biopsy from the same patient and genetically engineered to se...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20817
更新日期:2006-04-20 00:00:00
abstract::We have studied the growth rate dependence of hepatitis B surface antigen (HBsAg) p24(s) monomer and lipoprotein particle synthesis produced in Saccharomyces cerevisiae using galactose-limited continuous culture. The hepatitis B virus S gene, which encodes the p24(s) monomer, is transcribed under the control of the GA...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960305)49:5<578::AID-BIT
更新日期:1996-03-05 00:00:00
abstract::We present an evaluation of the qualitative and quantitative effects that high concentrations of benzene and toluene have on the growth rate of several pure cultures that use these compounds as their sole carbon and energy source. The cultures employed were five widely studied environmental isolates: Pseudomonas putid...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10729
更新日期:2003-09-30 00:00:00
abstract::We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26270
更新日期:2017-08-01 00:00:00
abstract::The laminate consisted of several polymer layers, aluminium, and one cellulose-based layer containing the active enzymatic system (e.g., glucose oxidase, catalase, glucose, and CaCO3). During the industrial lamination process, the enzyme layer was exposed to three temperature spikes up to 325 degrees C without signifi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10266
更新日期:2002-07-05 00:00:00
abstract::There has been a recent drive in commercial large-scale production of biotechnology products to convert current batch mode processing to continuous processing manufacturing. There have been reports of model systems capable of adapting and linking upstream and downstream technologies into a continuous manufacturing pip...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章,评审
doi:10.1002/bit.26060
更新日期:2017-01-01 00:00:00
abstract::Stem cell self-renewal versus differentiation fate decisions are difficult to characterize and analyze due to multiple competing rate processes occurring simultaneously among heterogeneous cell subpopulations. To address this challenge, we describe a mathematical model for cell population dynamics that allows flow cyt...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20244
更新日期:2004-11-05 00:00:00
abstract::Porcine pancreatic lipase and Fusarium solani cutinase were used to catalyze transesterification reactions between methyl propionate, ethyl propionate, and a series of primary alcohols at high temperatures in a continuous packed-bed gas-solid reactor, in which the solid phase is composed of the enzyme and the substrat...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390415
更新日期:1992-02-20 00:00:00
abstract::A MATLAB-based program was developed to simulate the distributions of states and behaviors of microbial storage product-accumulating bacteria in suspended growth systems. Currently available computer simulators of these systems predict dynamic behaviors by numerically solving differential biokinetic equations using av...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20484
更新日期:2005-07-05 00:00:00
abstract::Biomanufacturing exhibits inherent variability that can lead to variation in performance attributes and batch failure. To help ensure process consistency and product quality the development of predictive models and integrated control strategies is a promising approach. In this study, a feedback controller was develope...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27015
更新日期:2019-09-01 00:00:00
abstract::Enzymatic acrylation is a process of potentially strong interest to the chemical industry. Direct esterification involving acrylic acid is unfortunately rather slow, with inhibition phenomena appearing at high acid concentrations. In the present study the acrylation of 1-octanol catalyzed by immobilized Candida antarc...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21706
更新日期:2008-04-15 00:00:00
abstract::A novel feeding strategy that prolongs rapid growth rates for Escherichia coli fermentations to moderately high cell density is presented. High-density fermentations are a common and successful means of producing biological products. However, acetate accumulation can be a substantial problem in these procedures. To av...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20369
更新日期:2005-02-20 00:00:00
abstract::Current techniques for characterizing biofilm physiology lack the signal filtering capability required for quantifying signals associated with real time biologically active transport. Though a great deal was learned from previous investigations, no results have been reported on the characterization of in vivo, real ti...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22128
更新日期:2009-02-15 00:00:00
abstract::Peroxidase-catalyzed asymmetric sulfoxidations, while synthetically attractive, suffer from relatively low reaction rates due to poor substrate solubilities in water and from appreciable spontaneous oxidation of substrates (especially aryl alkyl sulfides) with H(2)O(2). In this work, we found that both of these shortc...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/1097-0290(20001105)70:3<353::aid-bit13>3.0
更新日期:2000-11-05 00:00:00
abstract::Embryonic stem cells (ESCs) can differentiate into all somatic cell types, but the development of effective strategies to direct ESC fate is dependent upon defining environmental parameters capable of influencing cell phenotype. ESCs are commonly differentiated via cell aggregates referred to as embryoid bodies (EBs),...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22578
更新日期:2010-02-15 00:00:00